Abstract：
:Sequences from the 5' end of type 1 human immunodeficiency virus RNA dimerize spontaneously in vitro in a reaction thought to mimic the initial step of genomic dimerization in vivo. Dimer initiation has been proposed to occur through a "kissing-loop" interaction involving a specific RNA stem-loop element designated SL1: the RNA strands first interact by base pairing through a six-base GC-rich palindrome in the loop of SL1, whose stems then isomerize to form a longer interstrand duplex. We now report a mutational analysis aimed at defining the features of SL1 RNA sequence and secondary structure required for in vitro dimer formation. Our results confirm that mutations which destroy complementarity in the SL1 loop abolish homodimer formation, but that certain complementary loop mutants can heterodimerize. However, complementarity was not sufficient to ensure dimerization, even between GC-rich loops, implying that specific loop sequences may be needed to maintain a conformation that is competent for initial dimer contact; the central GC pair of the loop palindrome appeared critical in this regard, as did two or three A residues which normally flank the palindrome. Neither the four-base bulge normally found in the SL1 stem nor the specific sequence of the stem itself was essential for the interaction; however, the stem structure was required, because interstrand complementarity alone did not support dimer formation. Electron microscopic analysis indicated that the RNA dimers formed in vitro morphologically resembled those isolated previously from retroviral particles. These results fully support the kissing-loop model and may provide a framework for systematically manipulating genomic dimerization in type 1 human immunodeficiency virus virions.
journal_name
J Viroljournal_title
Journal of virologyauthors
Clever JL,Wong ML,Parslow TGdoi
10.1128/JVI.70.9.5902-5908.1996subject
Has Abstractpub_date
1996-09-01 00:00:00pages
5902-8issue
9eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract：:Simian virus 40 tumor (T) antigen, an established viral oncoprotein, causes alterations in cell growth control through interacting with, and altering the function of, cellular proteins. To examine the effects of T antigen on cell growth control, and to identify the cellular proteins with which it may functionally inte...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.2.756-763.1995
更新日期：1995-02-01 00:00:00
abstract：:Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that produces an acute, lethal infection in rainbow trout (Oncorhynchus mykiss). Fish that survive infection cease to produce detectable infectious virus at approximately 46 days after infection, yet there is evidence that survivor fish continue to harbor...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.73.1.843-849.1999
更新日期：1999-01-01 00:00:00
abstract：:Recent studies have revealed that innate immunity is involved in the development of adaptive immune responses; however, its role in protection is not clear. In order to elucidate the exact role of Toll-like receptor (TLR) or RIG-I-like receptor (RLR) signaling on immunogenicity and protective efficacy against influenz...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01675-10
更新日期：2010-12-01 00:00:00
abstract：:Ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.16.2.447-452.1975
更新日期：1975-08-01 00:00:00
abstract：:The 86-kDa IE-2 protein of human cytomegalovirus is able to autoregulate its own expression via a short nucleotide sequence, termed the cis repression signal (CRS), that is located between the TATA box and the cap site of the IE-1/2 enhancer-promoter. Here we report that the 86-kDa IE-2 protein can interact directly w...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.67.1.323-331.1993
更新日期：1993-01-01 00:00:00
abstract：:Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the lef...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.1.320-329.1998
更新日期：1998-01-01 00:00:00
abstract：:Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orienta...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.59.2.494-499.1986
更新日期：1986-08-01 00:00:00
abstract：:The adenovirus type 5 mutant dl1520 was engineered previously to be completely defective for E1B-55K functions. Recently, this mutant (also known as ONYX-015) has been suggested to replicate preferentially in p53(-) and some p53(+) tumor cell lines but to be attenuated in primary cultured cells (C. Heise, A. Sampson-J...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.73.7.5333-5344.1999
更新日期：1999-07-01 00:00:00
abstract：:The EICP0 protein of equine herpesvirus 1 (EHV-1) is an early, viral regulatory protein that independently trans-activates EHV-1 immediate-early (IE), early, gamma1 late, and gamma2 late promoters. To assess whether this powerful trans-activator functions in conjunction with three other EHV-1 regulatory proteins to ac...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.3.1200-1208.2000
更新日期：2000-02-01 00:00:00
abstract：:The human immunodeficiency virus type 1 (HIV-1) entry inhibitor eCD4-Ig is a fusion of CD4-Ig and a coreceptor-mimetic peptide. eCD4-Ig is markedly more potent than CD4-Ig, with neutralization efficiencies approaching those of HIV-1 broadly neutralizing antibodies (bNAbs). However, unlike bNAbs, eCD4-Ig neutralized al...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02011-17
更新日期：2018-05-29 00:00:00
abstract：:There is a high degree of intraisolate sequence heterogeneity in the tax gene of human T-cell leukemia virus type I (HTLV-I), although the sequence variation between patients is small compared with that of human immunodeficiency virus type 1. In the present study, we investigated whether naturally occurring amino acid...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.4.2649-2653.1995
更新日期：1995-04-01 00:00:00
abstract：:Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.19.2.709-716.1976
更新日期：1976-08-01 00:00:00
abstract：:Bats have been identified as the natural reservoir of severe acute respiratory syndrome (SARS)-like and SARS coronaviruses (SLCoV and SCoV). However, previous studies suggested that none of the currently sampled bat SLCoVs is the descendant of the direct ancestor of SCoV, based on their relatively distant phylogenetic...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01926-07
更新日期：2008-02-01 00:00:00
abstract：:The retroviral restriction factor TRIMCyp, derived from the TRIM5 gene, blocks replication at a postentry step. TRIMCyp has so far been found in four species of Asian macaques, Macaca fascicularis, M. mulatta, M. nemestrina, and M. leonina. M. fascicularis is commonly used as a model for AIDS research, but TRIMCyp has...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00097-11
更新日期：2011-10-01 00:00:00
abstract：:Influenza A viruses (IAV) are lytic viruses that have recently been found to activate necroptosis in many of the cell types they infect. Necroptotic cell death is potently immunogenic and limits IAV spread by directly eliminating infected cells and by mobilizing both innate and adaptive immune responses. The benefits ...
journal_title：Journal of virology
pub_type： 杂志文章,评审
doi：10.1128/JVI.01101-19
更新日期：2020-04-16 00:00:00
abstract：:Evidence is presented that the herpes simplex virus type 2 glycoprotein previously designated gF is antigenically related to herpes simplex virus type 1 gC (gC-1). An antiserum prepared against type 1 virion envelope proteins immunoprecipitated gF of type 2 (gF-2), and competition experiments revealed that the anti-gC...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.47.3.553-562.1983
更新日期：1983-09-01 00:00:00
abstract：:The immediate-early gene ie2 of murine cytomegalovirus was characterized. The 1.75-kb ie2 transcript is spliced from three exons, of 78, 124, and 1,283 nucleotides, which are separated by introns of 1,245 and 364 nucleotides. An ATG codon located in the third exon leads into an open reading frame of 391 codons. Immedi...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.3.1638-1643.1991
更新日期：1991-03-01 00:00:00
abstract：:Zyxin, a focal adhesion molecule, interacts specifically with the E6 protein from human papillomavirus (HPV) type 6 in a yeast two-hybrid screen of a cDNA library prepared from human keratinocytes. Zyxin does not interact significantly with E6 proteins from HPV types 11, 16, or 18. The interaction was confirmed by in ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.75.23.11791-11802.2001
更新日期：2001-12-01 00:00:00
abstract：:We previously reported that the cellular transcription factor hypoxia-inducible factor 1α (HIF-1α) binds a hypoxia response element (HRE) located within the promoter of Epstein-Barr virus's (EBV's) latent-lytic switch BZLF1 gene, Zp, inducing viral reactivation. In this study, EBV-infected cell lines derived from gast...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00722-20
更新日期：2020-08-31 00:00:00
abstract：:MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identify miRNAs encoded by herpes simplex virus 1 (HSV-1), we applied a computational method to screen the complete genome of HSV-1 for seque...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00200-06
更新日期：2006-06-01 00:00:00
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journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.71.2.1443-1452.1997
更新日期：1997-02-01 00:00:00
abstract：:In vitro studies have suggested an important role for the minor capsid protein (VP1) unique region and the junction between VP1 and the major capsid protein (VP2) in the neutralizing immune response to B19 parvovirus. We investigated the role of the NH2-terminal region of the major structural protein in capsid structu...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.10.6567-6571.1995
更新日期：1995-10-01 00:00:00
abstract：:The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.2.1107-1114.1995
更新日期：1995-02-01 00:00:00
abstract：:Ribonucleic acid extracted from the virions of two human rhinoviruses is infective and is similar in size to poliovirus ribonucleic acid. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.7.2.278-280.1971
更新日期：1971-02-01 00:00:00
abstract：:By preannealing a radioactive, representative Moloney murine leukemia virus (M-MuLV) cDNA with large excesses of AKR 70S viral RNA, an M-MuLV-specific cDNA has been prepared. When hybridized to restriction enzyme fragments of M-MuLV-infected mouse cell DNA, the preannealed probe recognizes integrated M-MuLV DNA and do...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.33.3.1074-1082.1980
更新日期：1980-03-01 00:00:00
abstract：UNLABELLED:Inhibitor-of-apoptosis (IAP) proteins are key regulators of the innate antiviral response by virtue of their capacity to respond to signals affecting cell survival. In insects, wherein the host IAP provides a primary restriction to apoptosis, diverse viruses trigger rapid IAP depletion that initiates caspase...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.03659-14
更新日期：2015-04-01 00:00:00
abstract：:Three hypervariable regions were identified in a central portion of open reading frame 1 of TT virus DNA, which codes for a putative capsid protein of 770 amino acids. TT virus circulates as quasispecies, with many amino acid substitutions in hypervariable regions, to evade immune surveillance of the hosts and to esta...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.73.11.9604-9608.1999
更新日期：1999-11-01 00:00:00
abstract：:Cellular responses to DNA damage are crucial for maintaining genome integrity, virus infection, and preventing the development of cancer. Hepatitis C virus (HCV) infection and the expression of the HCV nonstructural protein NS3 and core protein have been proposed as factors involved in the induction of double-stranded...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00351-08
更新日期：2008-10-01 00:00:00
abstract：:This article has been retracted. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01824-07
更新日期：2008-01-23 00:00:00
abstract：:During the course of experiments designed to isolate deletion mutants of herpes simplex virus type 1 in the gene encoding the major DNA-binding protein, ICP8, a mutant, d61, that grew efficiently in ICP8-expressing Vero cells but not in normal Vero cells was isolated (P. K. Orberg and P. A. Schaffer, J. Virol. 61:1136...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.61.11.3528-3535.1987
更新日期：1987-11-01 00:00:00
