Abstract:
:In vitro studies have suggested an important role for the minor capsid protein (VP1) unique region and the junction between VP1 and the major capsid protein (VP2) in the neutralizing immune response to B19 parvovirus. We investigated the role of the NH2-terminal region of the major structural protein in capsid structure by expressing progressively more truncated versions of the VP2 gene followed by analysis using immunoblotting and electron microscopy of density gradient-purified particles. Deletion of the first 25 amino acids (aa) of VP2 did not affect capsid assembly. Altered VP2 with truncations to aa 26 to 30, including a single amino acid deletion at position 25, failed to self-assemble but did participate with normal VP2 in the capsid structure. The altered region corresponds to the beginning of the beta A antiparallel strand. Truncations beyond aa 30 were incompatible with either self-assembly or coassembly, probably because of deletion of the beta B strand, which helps to form the core structure of the virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kawase M,Momoeda M,Young NS,Kajigaya Sdoi
10.1128/JVI.69.10.6567-6571.1995subject
Has Abstractpub_date
1995-10-01 00:00:00pages
6567-71issue
10eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.51.3.650-655.1984
更新日期:1984-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.68.10.6684-6689.1994
更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.64.9.4232-4241.1990
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00748-16
更新日期:2016-09-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4381-4389.1995
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pub_type: 杂志文章
doi:10.1128/JVI.4.5.567-573.1969
更新日期:1969-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00072-10
更新日期:2010-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.137-142.1990
更新日期:1990-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.133-144.1985
更新日期:1985-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2000-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.70.9.5827-5831.1996
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pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4534-4539.1990
更新日期:1990-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2007-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3257-3266.2002
更新日期:2002-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.66.9.5224-5231.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02170-14
更新日期:2015-01-01 00:00:00
abstract::Herpes simplex virus establishes latent infections in the nuclei of sensory neurons. These infections are characterized by the abundant expression of a series of 5' coterminal transcripts termed the latency-associated transcripts (LATs). Available evidence indicates that LAT expression is specifically regulated in lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7424-7432.1996
更新日期:1996-11-01 00:00:00
abstract::The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.3.534-539.1975
更新日期:1975-03-01 00:00:00