Abstract:
:We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the Gag polyproteins tested, only those encoded by closely related retroviruses formed heteromultimers. To determine the primary sequence requirements for human immunodeficiency virus type 1 Gag polyprotein multimerization, we studied the effects on multimerization of deletion and linker insertion mutations. Sequences necessary for this process were located between the C-terminal one-third of the capsid domain and the C terminus of the nucleocapsid domain.
journal_name
J Viroljournal_title
Journal of virologyauthors
Franke EK,Yuan HE,Bossolt KL,Goff SP,Luban Jdoi
10.1128/JVI.68.8.5300-5305.1994subject
Has Abstractpub_date
1994-08-01 00:00:00pages
5300-5issue
8eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
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