Abstract:
:Tryptic digests of four polypeptides found in Kunjin virus-infected Vero cells, NV5, NV4, V3, and NV3, were compared by peptide mapping. The polypeptides to be analyzed were labeled with radioactive methionine and separated by electrophoresis through polyacrylamide gels containing sodium dodecyl sulfate. Because infection of Vero cells by Kunjin virus does not inhibit host cell protein synthesis, radioactively labeled viral polypeptides prepared from infected cells migrate coincidentally during sodium dodecyl sulfate-gel electrophoresis with some of the labeled host proteins. Thus, the genuine viral methionine-containing peptides in tryptic digests of viral proteins have been identified by co-analyzing polypeptides from [3H]methionine-labeled uninfected cells and [35S]methionine-labeled infected cells and determining the 35S/3H ratio in the peptides resolved in two dimensions on thin-layer chromatography plates. The peptide map of NV3 demonstrated that it is host coded, whereas NV5, NV4, and V3 have unique peptide maps and, therefore, account for approximately one-half of the coding potential of Kunjin virus RNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wright PJ,Bowden DS,Westaway EGdoi
10.1128/JVI.24.2.651-661.1977subject
Has Abstractpub_date
1977-11-01 00:00:00pages
651-61issue
2eissn
0022-538Xissn
1098-5514journal_volume
24pub_type
杂志文章abstract::The identification of tumor-inducing genes is a driving force for elucidating the molecular mechanisms underlying cancer. Many retroviruses induce tumors by insertion of viral DNA adjacent to cellular oncogenes, resulting in altered expression and/or structure of the encoded proteins. The availability of the mouse gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2056-2062.2003
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00152-06
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
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journal_title:Journal of virology
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journal_title:Journal of virology
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doi:10.1128/JVI.72.1.57-64.1998
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journal_title:Journal of virology
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更新日期:1974-07-01 00:00:00
abstract:UNLABELLED:The HIV-1 glycoprotein 41 promotes fusion of the viral membrane with that of the target cell. Structural, biochemical, and biophysical studies suggest that its membrane-proximal external region (MPER) may interact with the HIV-1 membrane and induce its disruption and/or deformation during the process. Howeve...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.163-171.1982
更新日期:1982-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1980-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00