Abstract:
:The early proteins of simian virus 40 (SV40) large T and small t antigen (T/t antigen) can each cause the transcriptional activation of a variety of cellular and viral promoters. We showed previously that simian cellular DNA-binding factors (the Band A factors) bind to sequences within the SV40 late promoter which are important for transcriptional activation in the presence of the SV40 early proteins. Band A factors isolated from simian cells which produce T/t antigen (COS cells or SV40-infected CV-1 cells) have altered binding properties in comparison with the factors from normal simian cells (CV-1). This suggests that the transcriptional activation mediated by T/t antigen may be due to either modification of existing factors or induction of new members of a family of factors. We have purified the Band A factors from both COS and CV-1 cells and have determined the binding site by methylation interference and DNase protection footprinting. The COS cell factors have altered chromatographic properties on ion-exchange columns and have higher-molecular-weight forms than the CV-1 cell factors. Major forms of the CV-1 factors migrate between 20 and 24 kilodaltons, while the COS factors migrate between 20 and 28 kilodaltons. The binding sites for the factors from CV-1 and COS cells are similar, covering a rather broad region within the 72-base-pair repeat comprising the AP-1 site and the two-octamer binding protein (OBP100/Oct 1) sites, OBP I and OBP II. Specific binding competition analyses indicate that the two general regions within the binding site (the AP-1-OBP II site and the OBP I site) each retain partial binding ability; however, the factors bind best when the two regions are adjacent in a relatively specific spatial arrangement. The binding site for the Band A factors corresponds very well to sequences necessary for the activation of the late promoter as defined by deletion and base substitution mutagenesis studies (J. M. Keller and J. C. Alwine, Mol. Cell. Biol. 5:1859-1869, 1985; E. May, F. Omilli, M. Emoult-Lange, M. Zenke, and P. Chambon, Nucleic Acids Res. 15:2445-2461, 1987). These data, in combination with the data showing that the Band A factors are modified or induced in the presence of T/t antigen, strongly suggest that T/t antigen mediates its transcriptional activation function, at least in part, through the Band A factors.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gallo GJ,Gruda MC,Manuppello JR,Alwine JCdoi
10.1128/JVI.64.1.173-184.1990subject
Has Abstractpub_date
1990-01-01 00:00:00pages
173-84issue
1eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The small defective T particle of vesicular stomatitis virus (VSV-111) has no detectable enzyme activity when assayed at 31 C, although qualitatively it possesses all the virion proteins found in complete VSV-1 virions. With VSV-1 transcription product ribonucleic acid (RNA), it is shown that the VSV-111 RNA is identi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.946-955.1972
更新日期:1972-06-01 00:00:00
abstract::By means of both electron microscopy and plaque assay techniques, 10(-3)m Zn(2+) has been shown to reduce the adsorption rate of male-specific filamentous deoxyribonucleic acid bacteriophages to the tips of F pili. In contrast, 10(-3)m Zn(2+) did not affect the adsorption of male-specific ribonucleic acid phages to th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.869-871.1972
更新日期:1972-10-01 00:00:00
abstract::The processes that facilitate transport of integral membrane proteins though the secretory pathway and subsequently target them to particular cellular membranes are relevant to almost every field of biology. These transport processes involve integration of proteins into the membrane of the endoplasmic reticulum (ER), ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12528-12535.2005
更新日期:2005-10-01 00:00:00
abstract:UNLABELLED:Kaposi's sarcoma-associated herpesvirus (KSHV) typically displays two different phases in its life cycle, the default latent phase and the lytic phase. There is a short period of lytic gene expression in the early stage of KSHV primary infection. The factors involved in the shutdown process of lytic gene exp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00596-14
更新日期:2014-07-01 00:00:00
abstract::Since HIV-1 replication is modulated at multiple stages by host cell factors, identification and characterization of those host cell factors are expected to contribute to the development of novel anti-HIV therapeutics. Previous studies showed that a C-terminally truncated cytosolic form of cleavage and polyadenylation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00124-13
更新日期:2013-07-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential protein. Previous studies have shown that the UL25 gene product is a virion component (M. A. Ali et al., Virology 216:278-283, 1996) involved in virus penetration and capsid assembly (C. Addison e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
abstract::The nucleotide sequence corresponding to the P3 region of the hepatitis A virus (HAV) polyprotein genome was determined from cloned cDNA and translated into an amino acid sequence. Comparison of the amino acid sequences of the genome-linked proteins (VPgs) of other picornaviruses with the predicted amino acid sequence...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.124-130.1986
更新日期:1986-10-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen affecting an estimated 3% of the world's population. Recent advances have enabled in vitro propagation of the virus and allow assembly and egress to be investigated for the first time. As a component of the virion, the HCV core protein likely functions primarily i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00793-07
更新日期:2007-10-01 00:00:00
abstract::3-O-(3',3'-dimethylsuccinyl)betulinic acid (PA-457 or bevirimat) potently inhibits human immunodeficiency virus type 1 (HIV-1) maturation by blocking a late step in the Gag processing pathway, specifically the cleavage of SP1 from the C terminus of capsid (CA). To gain insights into the mechanism(s) by which HIV-1 cou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01369-06
更新日期:2006-11-01 00:00:00
abstract::Paramyxoviruses initiate entry through the concerted action of the tetrameric attachment glycoprotein (HN, H, or G) and the trimeric fusion glycoprotein (F). The ectodomains of HN/H/G contain a stalk region important for oligomeric stability and for the F triggering resulting in membrane fusion. Paramyxovirus HN, H, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00076-12
更新日期:2012-06-01 00:00:00
abstract::Viruses cause about 15% of the cancers that are still the leading causes of human mortality. The discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. However, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. The present study has a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01392-10
更新日期:2011-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 uses ribosomal frameshifting for translation of the Gag-Pol polyprotein. Frameshift activities are thought to be tightly regulated. Analysis of gag p1 sequences from 270 plasma virions identified in 64% of the samples the occurrence of polymorphism that could lead to changes in ther...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7868-7873.2002
更新日期:2002-08-01 00:00:00
abstract::A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12232-12242.2003
更新日期:2003-11-01 00:00:00
abstract::Encephalomyocarditis (EMC) virus (K-2 strain) was separated into two distinct peaks of optical density by chromatography on calcium phosphate, eluting at 0.17 and 0.31 m, respectively. The virus in these peaks could not be distinguished by infectivity (plaque-forming units), hemagglutinin activity, electron microscopi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.1.2.308-316.1967
更新日期:1967-04-01 00:00:00
abstract::Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines express...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.8.3732-3742.2006
更新日期:2006-04-01 00:00:00
abstract::One common attribute of herpesviruses is the ability to establish latent, life-long infections. The role of virus-virus interaction in viral reactivation between or among herpesviruses has not been studied. Preliminary experiments in our laboratory had indicated that infection of Epstein-Barr virus (EBV) genome-positi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6768-6777.1993
更新日期:1993-11-01 00:00:00
abstract::The involvement of mouse mammary tumor virus (MTV) in the development of mammary tumors of nonviral etiology in BALB/c mice was studied by measuring the levels of MTV RNA, MTV DNA, and MTV proteins in spontaneously arising and hormonally, chemically, and/or physically induced mammary tumors of BALB/c females. The foll...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.1.63-72.1979
更新日期:1979-07-01 00:00:00
abstract::Five minority populations of aberrant, varicella-zoster virus (VZV)-derived genomes were identified among the encapsidated DNAs obtained from the nuclear and cytoplasmic fractions of an in vitro infection initiated with a lyophilized sample of the BIKEN VZV vaccine (strain Oka). These were (i) VZV genomes, present wit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.92-101.1985
更新日期:1985-10-01 00:00:00
abstract::Replication of the genomic RNA of severe acute respiratory syndrome coronavirus (SARS-CoV) is mediated by replicase polyproteins that are processed by two viral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro). Previously, we showed that SARS-CoV PLpro processes the replicase polyprotein at three ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15189-15198.2005
更新日期:2005-12-01 00:00:00
abstract::Thanks to new technologies which enable rapid and unbiased screening for viral nucleic acids in clinical specimens, an impressive number of previously unknown viruses have recently been discovered. Two research groups independently identified a novel negative-strand RNA virus, now designated avian bornavirus (ABV), in...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.02567-09
更新日期:2010-07-01 00:00:00
abstract::Adeno-associated virus type 2 (AAV-2) gene expression is tightly controlled by functions of the helper virus as well as by the products of its own viral rep gene. Double-immunofluorescence studies of Rep and VP protein expression in cells coinfected with AAV-2 and adenovirus type 2 showed that a large proportion of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8437-8447.1997
更新日期:1997-11-01 00:00:00
abstract:UNLABELLED:The ORF45 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus-specific immediate-early tegument protein. Our previous studies have revealed its crucial roles in both early and late stages of KSHV infection. In this study, we surveyed the interactome of ORF45 using a panel of monoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02925-14
更新日期:2015-05-01 00:00:00
abstract::A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis of Sendai virus was incubated with the precursor to Escherichia coli tRNA Tyr. The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU. Like...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.274-284.1978
更新日期:1978-01-01 00:00:00
abstract::The E8 open reading frame of bovine papillomavirus type 4 encodes a small hydrophobic polypeptide which contributes to cell transformation by conferring anchorage-independent growth. Using an in vitro translation system, we show that the E8 polypeptide binds to ductin, the 16-kDa proteolipid that forms transmembrane c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.9041-9045.1996
更新日期:1996-12-01 00:00:00
abstract::The structural instability of inactivated foot-and-mouth disease virus (FMDV) hinders the development of vaccine industry. Here we found that some transition metal ions like Cu2+ and Ni2+ could specifically bind to FMDV capsids at capacities about 7089 and 3448 metal ions per capsid, respectively. These values are abo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02431-20
更新日期:2021-01-13 00:00:00
abstract::The phylogeny of reptilian herpesviruses (HVs) relative to mammalian and avian HVs was investigated by using available gene sequences and by alignment of encoded amino acid sequences and derivation of trees by maximum-likelihood and Bayesian methods. Phylogenetic loci were obtained for green turtle HV (GTHV) primarily...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.725-731.2005
更新日期:2005-01-01 00:00:00
abstract::The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.151-160.1975
更新日期:1975-01-01 00:00:00
abstract::We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.90-96.1986
更新日期:1986-10-01 00:00:00
abstract::Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3779-3788.1988
更新日期:1988-10-01 00:00:00
abstract::Posttranscriptional gene silencing (PTGS), a homology-dependent RNA degradation system, has a role in defending against virus infection in plants, but plant viruses encode a suppressor to combat PTGS. Using transgenic tobacco in which the expression of green fluorescent protein (GFP) is posttranscriptionally silenced,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.11016-11026.2003
更新日期:2003-10-01 00:00:00