Abstract:
:Posttranscriptional gene silencing (PTGS), a homology-dependent RNA degradation system, has a role in defending against virus infection in plants, but plant viruses encode a suppressor to combat PTGS. Using transgenic tobacco in which the expression of green fluorescent protein (GFP) is posttranscriptionally silenced, we investigated a tomato mosaic virus (ToMV)-encoded PTGS suppressor. Infection with wild-type ToMV (L strain) interrupted GFP silencing in tobacco, coincident with visible symptoms, whereas some attenuated strains of ToMV (L(11) and L(11)A strains) failed to suppress GFP silencing. Analyses of recombinant viruses containing the L and L(11)A strains revealed that a single base change in the replicase gene, which causes an amino acid substitution, is responsible for the symptomless and suppressor-defective phenotypes of the attenuated strains. An agroinfiltration assay indicated that the 130K replication protein acts as a PTGS suppressor. Small interfering RNAs (siRNAs) of 21 to 25 nucleotides accumulated during ToMV infection, suggesting that the major target of the ToMV-encoded suppressor is downstream from the production of siRNAs in the PTGS pathway. Analysis with GFP-tagged recombinant viruses revealed that the suppressor inhibits the establishment of the ToMV-targeted PTGS system in the inoculated leaves but does not detectably suppress the activity of the preexisting, sequence-specific PTGS machinery there. Taken together, these results indicate that it is likely that the ToMV-encoded suppressor, the 130K replication protein, blocks the utilization of silencing-associated small RNAs, so that a homology-dependent RNA degradation machinery is not newly formed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kubota K,Tsuda S,Tamai A,Meshi Tdoi
10.1128/jvi.77.20.11016-11026.2003subject
Has Abstractpub_date
2003-10-01 00:00:00pages
11016-26issue
20eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract::Herpes simplex virus establishes latent infections in the nuclei of sensory neurons. These infections are characterized by the abundant expression of a series of 5' coterminal transcripts termed the latency-associated transcripts (LATs). Available evidence indicates that LAT expression is specifically regulated in lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7424-7432.1996
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abstract::The nonpathogenic human defective parvovirus adeno-associated virus (AAV) type 2 induced differentiation-associated antigens in cells of the human leukemia cell line HL60 (CD 67), as well as in two different lines of immortalized human keratinocytes, HaCaT and HPK Ia cells (involucrin and cytokeratin 10). Simultaneous...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4191-4200.1992
更新日期:1992-07-01 00:00:00
abstract:UNLABELLED:Lifelong antiretroviral therapy (ART) for HIV-1 does not diminish the established latent reservoir. A possible cure approach is to reactivate the quiescent genome from latency and utilize immune responses to eliminate cells harboring reactivated HIV-1. It is not known whether antibodies within HIV-1-infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02717-15
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.194-200.1985
更新日期:1985-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5133-5141.1989
更新日期:1989-12-01 00:00:00
abstract::An in vitro system for production of hepatitis B virus (HBV) was established by infection of human hepatoma (HepG2) cells. HBV particles obtained from the serum of a chronic hepatitis B surface antigen (subtype ad) carrier were used to inoculate HepG2 cells. HBV envelope and core proteins were synthesized de novo by t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3025-3032.1990
更新日期:1990-06-01 00:00:00
abstract::In this report, we describe some phenotypic properties of a temperature-sensitive mutant of herpes simplex type 1 (HSV-1) and present data concerning the physical location and nucleotide sequence of the genomic region harboring the mutation. The effect of shifts from the permissive to the nonpermissive temperature on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2169-2179.1989
更新日期:1989-05-01 00:00:00
abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus which requires adenovirus as a helper for productive infection. We studied whether intracellular AAV DNA in KB cells was present in a chromatin-like structure by digesting infected cell nuclei with micrococcal nuclease. Virus DNA was detected by agarose gel e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.1.79-87.1983
更新日期:1983-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00650-12
更新日期:2012-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01748-14
更新日期:2014-09-01 00:00:00
abstract::The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 eleme...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01719-08
更新日期:2009-01-01 00:00:00
abstract::The presence of porcine endogenous retroviruses presents a potential risk of transmission of infectious diseases (xenozoonosis) if tissues and organs from genetically modified pigs are to be used in xenotransplantation. Here, we report that intracellular expression of a llama single-domain antibody against p15, the ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.22.12132-12139.2003
更新日期:2003-11-01 00:00:00
abstract::The G glycoprotein of human respiratory syncytial virus (RSV) was identified previously as the viral attachment protein. Although we and others recently showed that G is not essential for replication in vitro, it does affect the efficiency of replication in a cell type-dependent fashion and is required for efficient r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6164-6171.2002
更新日期:2002-06-01 00:00:00
abstract::Due to the biological significance of the carbohydrate component of the human immunodeficiency virus type 1 (HIV-1) glycoproteins in viral pathogenesis, the glycosylation step constitutes an attractive target for anti-HIV therapy. Cyanovirin N (CV-N), which specifically targets the high-mannose (HM) glycans on gp120, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7777-7784.2005
更新日期:2005-06-01 00:00:00
abstract::Prior immunity to influenza A virus (IAV) in mice changes the outcome to a subsequent lymphocytic choriomeningitis virus (LCMV) infection and can result in severe lung pathology, similar to that observed in patients that died of the 1918 H1N1 pandemic. This pathology is induced by IAV-specific memory CD8(+) T cells cr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00936-13
更新日期:2013-12-01 00:00:00
abstract::A number of herpes simplex virus (HSV) glycoproteins are found in oligomeric states: glycoprotein E (gE)-gI and gH-gL form heterodimers, and both gB and gC have been detected as homodimers. We have further explored the organization of glycoproteins in the virion envelope by using both purified virions to quantitate gl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6067-6070.1996
更新日期:1996-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.23.11880-11888.2002
更新日期:2002-12-01 00:00:00
abstract::Among Moloney murine leukemia viruses (Mo-MuLVs) having stop codons other than UAG at the gag-pol junction, Mo-MuLV with UAA, but not with UGA, had a replication disadvantage. Mo-MuLV with a glutamine codon (CAG) at the junction did not replicate. A revertant of this virus consisted of the original virus and a virus w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6376-6379.1991
更新日期:1991-11-01 00:00:00
abstract::Arenaviruses are negative-strand RNA viruses that cause human diseases such as lymphocytic choriomeningitis, Bolivian hemorrhagic fever, and Lassa hemorrhagic fever. No licensed vaccines exist, and current treatment is limited to ribavirin. The prototypic arenavirus, lymphocytic choriomeningitis virus (LCMV), is a mod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02081-10
更新日期:2011-04-01 00:00:00
abstract::Human bocavirus 1 (HBoV1) is a human parvovirus that causes acute respiratory tract infections in young children. In this study, we confirmed that, when polarized/well-differentiated human airway epithelia are infected with HBoV1 in vitro, they develop damage characterized by barrier function disruption and cell hypot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01533-17
更新日期:2017-11-30 00:00:00
abstract::Infection of microglia and other cells of the macrophage/monocyte lineage in the central nervous system (CNS) by human immunodeficiency virus type I (HIV-1) underlies the development of giant cell encephalitis (GCE). It is currently unknown whether GCE depends on the emergence of virus populations specifically adapted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11686-11699.2001
更新日期:2001-12-01 00:00:00
abstract::Following the initial detection of viral infection, innate immune responses trigger the induction of numerous interferon-stimulated genes (ISGs) to inhibit virus replication and dissemination. One such ISG encodes cholesterol-25-hydroxylase (CH25H), an enzyme that catalyzes the oxidation of cholesterol to form a solub...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01047-18
更新日期:2018-08-29 00:00:00
abstract::Herpes simplex virus virions contain one or more functions which mediate shutoff of host protein synthesis, disaggregation of host polyribosomes, and degradation of host mRNA. We studied aspects of the host shutoff mechanism by using herpes simplex virus type 1 mutants deficient in virion-induced shutoff of host prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2198-2207.1987
更新日期:1987-07-01 00:00:00
abstract::We characterized a number of important features of the structure of the cohesive overlap region of the DNA genome of duck hepatitis B virus. The 5'-terminal nucleotide of minus-strand DNA was localized to nucleotide 2537, a G residue within the 12-base repeat sequence DR1. This G residue was shown to be the site of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
abstract::In December 2016, a low-pathogenic avian influenza (LPAI) A(H7N2) virus was identified to be the causative source of an outbreak in a cat shelter in New York City, which subsequently spread to multiple shelters in the states of New York and Pennsylvania. One person with occupational exposure to infected cats became in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-17
更新日期:2017-07-12 00:00:00
abstract::H9N2 avian influenza virus (AIV) has an extended host range, but the molecular basis underlying H9N2 AIV transmission to mammals remains unclear. We isolated more than 900 H9N2 AIVs in our 3-year surveillance in live bird markets in China from 2009 to 2012. Thirty-seven representative isolates were selected for furthe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01141-16
更新日期:2016-10-14 00:00:00
abstract::Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A gene can be introduced by either infection or transfection, and either of the two major E1A proteins, 289R or 243R, can in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1236-1244.1991
更新日期:1991-03-01 00:00:00
abstract::The phosphoprotein pp65 (ppUL83) of human cytomegalovirus (HCMV) is abundantly synthesized during lytic infection in cultured fibroblasts. As a major constituent of extracellular particles, it gains entry to infected cells immediately after adsorption and subsequently translocates to the cell nucleus. This efficient t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.5959-5968.1995
更新日期:1995-10-01 00:00:00
abstract::At mucosal sites, papillomavirus genomes can persist in the epithelial basal layer following immune-mediated regression. Subsequent T-cell depletion stimulates a 3- to 5-log increase in the viral copy number, to levels associated with productive infection. Reappearance of microlesions was rare within the short time fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02589-13
更新日期:2014-01-01 00:00:00
abstract::We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5300-5305.1994
更新日期:1994-08-01 00:00:00