Strain specificity and binding affinity requirements of neutralizing monoclonal antibodies to the C4 domain of gp120 from human immunodeficiency virus type 1.

Abstract:

:The binding properties of seven CD4-blocking monoclonal antibodies raised against recombinant gp120 of human immunodeficiency virus type 1 strain MN (HIV-1MN) and two CD4-blocking monoclonal antibodies to recombinant envelope glycoproteins gp120 and gp160 of substrain IIIB of HIVLAI were analyzed. With a panel of recombinant gp120s from seven diverse HIV-1 isolates, eight of the nine antibodies were found to be strain specific and one was broadly cross-reactive. Epitope mapping revealed that all nine antibodies bound to epitopes located in the fourth conserved domain (C4) of gp120. Within this region, three distinct epitopes could be identified: two were polymorphic between HIV-1 strains, and one was highly conserved. Studies with synthetic peptides demonstrated that the conserved epitope, recognized by antibody 13H8, was located between residues 431 and 439. Site-directed mutagenesis of gp120 demonstrated that residue 429 and/or 432 was critical for the binding of the seven antibodies to gp120 from HIV-1MN. Similarly, residues 423 and 429 were essential for the binding of monoclonal antibody 5C2 raised against gp120 from HIV-1IIIB. The amino acids located at positions 423 and 429 were found to vary between strains of HIV-1 as well as between molecular clones derived from the MN and LAI isolates of HIV-1. Polymorphism at these positions prevented the binding of virus-neutralizing monoclonal antibodies and raised the possibility that HIV-1 neutralization serotypes may be defined on the basis of C4 domain sequences. Analysis of the binding characteristics of the CD4-blocking antibodies demonstrated that their virus-neutralizing activity was directly proportional to their gp120-binding affinity. These studies account for the strain specificity of antibodies to the C4 domain of gp120 and demonstrate for the first time that antibodies to this region can be as effective as those directed to the principal neutralizing determinant (V3 domain) in neutralizing HIV-1 infectivity.

journal_name

J Virol

journal_title

Journal of virology

authors

Nakamura GR,Byrn R,Wilkes DM,Fox JA,Hobbs MR,Hastings R,Wessling HC,Norcross MA,Fendly BM,Berman PW

doi

10.1128/JVI.67.10.6179-6191.1993

subject

Has Abstract

pub_date

1993-10-01 00:00:00

pages

6179-91

issue

10

eissn

0022-538X

issn

1098-5514

journal_volume

67

pub_type

杂志文章
  • Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.

    abstract::Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.61.4.1248-1252.1987

    authors: Neary K,Horwitz BH,DiMaio D

    更新日期:1987-04-01 00:00:00

  • The amphotropic murine leukemia virus receptor gene encodes a 71-kilodalton protein that is induced by phosphate depletion.

    abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.6.4564-4570.1997

    authors: Chien ML,Foster JL,Douglas JL,Garcia JV

    更新日期:1997-06-01 00:00:00

  • Measles virus attenuation associated with transcriptional impediment and a few amino acid changes in the polymerase and accessory proteins.

    abstract::Measles virus (MV) isolated in B95a cells, a marmoset B-cell line, retains full pathogenicity for cynomolgus monkeys, while its derivative obtained by adaptation to the growth in Vero cells, a monkey kidney cell line, loses the pathogenic potential (F. Kobune, H. Sakata, and A. Sugiura, J. Virol. 64:700-705, 1990). He...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.11.8690-8696.1998

    authors: Takeda M,Kato A,Kobune F,Sakata H,Li Y,Shioda T,Sakai Y,Asakawa M,Nagai Y

    更新日期:1998-11-01 00:00:00

  • Maturation of the cellular and humoral immune responses to persistent infection in horses by equine infectious anemia virus is a complex and lengthy process.

    abstract::Equine infectious anemia virus (EIAV) provides a natural model system by which immunological control of lentivirus infections may be studied. To date, no detailed study addressing in parallel both the humoral and cellular immune responses induced in horses upon infection by EIAV has been conducted. Therefore, we initi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.5.3840-3852.1997

    authors: Hammond SA,Cook SJ,Lichtenstein DL,Issel CJ,Montelaro RC

    更新日期:1997-05-01 00:00:00

  • Crystal structure of Marburg virus VP24.

    abstract::The VP24 protein plays an essential, albeit poorly understood role in the filovirus life cycle. VP24 is only 30% identical between Marburg virus and the ebolaviruses. Furthermore, VP24 from the ebolaviruses is immunosuppressive, while that of Marburg virus is not. The crystal structure of Marburg virus VP24, presented...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03565-13

    authors: Zhang AP,Bornholdt ZA,Abelson DM,Saphire EO

    更新日期:2014-05-01 00:00:00

  • Transfection of Escherichia coli spheroplasts. I. General facilitation of double-stranded deoxyribonucleic acid infectivity by protamine sulfate.

    abstract::The addition of 25 mug of protamine sulfate per ml to lysozyme-ethylenediamine-tetraacetic acid spheroplasts of Escherichia coli stimulates transfection not only for T1 phage deoxyribonucleic acid (DNA; Hotz and Mauser, 1969) but also for the following phage DNA species: lambda, 10,000-fold to an efficiency of 10(-3) ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.7.5.646-650.1971

    authors: Benzinger R,Kleber I,Huskey R

    更新日期:1971-05-01 00:00:00

  • Cell penetration and trafficking of polyomavirus.

    abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.4.2615-2622.2003

    authors: Gilbert JM,Goldberg IG,Benjamin TL

    更新日期:2003-02-01 00:00:00

  • In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.

    abstract::An adeno-associated virus (AAV) genome with a Lys-to-His (K340H) mutation in the consensus nucleotide triphosphate binding site of the rep gene has a dominant-negative DNA replication phenotype in vivo. We expressed both wild-type (Rep78) and mutant (Rep78NTP) proteins in two helper-free expression systems consisting ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.2.1236-1240.1992

    authors: Owens RA,Carter BJ

    更新日期:1992-02-01 00:00:00

  • The herpes simplex virus type 1 latency-associated transcript promoter is activated through Ras and Raf by nerve growth factor and sodium butyrate in PC12 cells.

    abstract::Herpes simplex virus establishes latent infections in the nuclei of sensory neurons. These infections are characterized by the abundant expression of a series of 5' coterminal transcripts termed the latency-associated transcripts (LATs). Available evidence indicates that LAT expression is specifically regulated in lat...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.11.7424-7432.1996

    authors: Frazier DP,Cox D,Godshalk EM,Schaffer PA

    更新日期:1996-11-01 00:00:00

  • Implication of a central cysteine residue and the HHCC domain of Moloney murine leukemia virus integrase protein in functional multimerization.

    abstract::Moloney murine leukemia virus (M-MuLV) IN-IN protein interactions important for catalysis of strand transfer and unimolecular and bimolecular disintegration reactions were investigated by using a panel of chemically modified M-MuLV IN proteins. Functional complementation of an HHCC-deleted protein (Ndelta105) by an in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.2.1691-1698.1998

    authors: Donzella GA,Leon O,Roth MJ

    更新日期:1998-02-01 00:00:00

  • Inhibition of Histone Deacetylase Activity Aggravates Coxsackievirus B3-Induced Myocarditis by Promoting Viral Replication and Myocardial Apoptosis.

    abstract:UNLABELLED:Viral myocarditis, which is most prevalently caused by coxsackievirus B3 (CVB3), is a serious clinical condition characterized by excessive myocardial inflammation. Recent studies suggest that regulation of protein acetylation levels by inhibiting histone deacetylase (HDAC) activity modulates inflammatory re...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01028-15

    authors: Zhou L,He X,Gao B,Xiong S

    更新日期:2015-10-01 00:00:00

  • Vaccinia virus DNA ligase recruits cellular topoisomerase II to sites of viral replication and assembly.

    abstract::Vaccinia virus replication is inhibited by etoposide and mitoxantrone even though poxviruses do not encode the type II topoisomerases that are the specific targets of these drugs. Furthermore, one can isolate drug-resistant virus carrying mutations in the viral DNA ligase and yet the ligase is not known to exhibit sen...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02723-07

    authors: Lin YC,Li J,Irwin CR,Jenkins H,DeLange L,Evans DH

    更新日期:2008-06-01 00:00:00

  • Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells.

    abstract::The natural life cycle of alphaviruses, a group of plus-strand RNA viruses, involves transmission to vertebrate hosts via mosquitoes. Chronic infections are established in mosquitoes (and usually in mosquito cell cultures), but infection of susceptible vertebrate cells typically results in rapid shutoff of host mRNA t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.5.3854-3865.1999

    authors: Frolov I,Agapov E,Hoffman TA Jr,Prágai BM,Lippa M,Schlesinger S,Rice CM

    更新日期:1999-05-01 00:00:00

  • In vitro selection and characterization of human immunodeficiency virus type 1 variants with increased resistance to ABT-378, a novel protease inhibitor.

    abstract::ABT-378, a new human immunodeficiency virus type 1 (HIV-1) protease inhibitor which is significantly more active than ritonavir in cell culture, is currently under investigation for the treatment of AIDS. Development of viral resistance to ABT-378 in vitro was studied by serial passage of HIV-1 (pNL4-3) in MT-4 cells....

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.9.7532-7541.1998

    authors: Carrillo A,Stewart KD,Sham HL,Norbeck DW,Kohlbrenner WE,Leonard JM,Kempf DJ,Molla A

    更新日期:1998-09-01 00:00:00

  • Conserved GXXXG- and S/T-like motifs in the transmembrane domains of NS4B protein are required for hepatitis C virus replication.

    abstract::Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02298-10

    authors: Han Q,Aligo J,Manna D,Belton K,Chintapalli SV,Hong Y,Patterson RL,van Rossum DB,Konan KV

    更新日期:2011-07-01 00:00:00

  • Regulation of protein synthesis in vesicular stomatitis virus-infected mouse L-929 cells by decreased protein synthesis initiation factor 2 activity.

    abstract::Infection of mouse L-cell spinner cultures by vesicular stomatitis virus (VSV) effected the selective translation of viral mRNA by 4h after viral adsorption. Cell-free systems prepared from mock- and VSV-infected cells reflected this phenomenon; protein synthesis was reduced in the virus-infected cell lysate by approx...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.41.3.781-791.1982

    authors: Centrella M,Lucas-Lenard J

    更新日期:1982-03-01 00:00:00

  • Stabilization but not the transcriptional activity of herpes simplex virus VP16-induced complexes is evolutionarily conserved among HCF family members.

    abstract::The human herpes simplex virus (HSV) protein VP16 induces formation of a transcriptional regulatory complex with two cellular factors-the POU homeodomain transcription factor Oct-1 and the cell proliferation factor HCF-1-to activate viral immediate-early-gene transcription. Although the cellular role of Oct-1 in trans...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.24.12402-12411.2001

    authors: Lee S,Herr W

    更新日期:2001-12-01 00:00:00

  • Intermonomer disulfide bonds impair the fusion activity of influenza virus hemagglutinin.

    abstract::At a low pH, the influenza virus hemagglutinin (HA) undergoes conformational changes that promote membrane fusion. While the critical role of fusion peptide release from the trimer interface has been demonstrated previously, the role of globular head dissociation in the overall fusion mechanism remains unclear. To inv...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.8.4940-4950.1992

    authors: Kemble GW,Bodian DL,Rosé J,Wilson IA,White JM

    更新日期:1992-08-01 00:00:00

  • Exploring the Human-Nipah Virus Protein-Protein Interactome.

    abstract::Nipah virus is an emerging, highly pathogenic, zoonotic virus of the Paramyxoviridae family. Human transmission occurs by close contact with infected animals, the consumption of contaminated food, or, occasionally, via other infected individuals. Currently, we lack therapeutic or prophylactic treatments for Nipah viru...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01461-17

    authors: Martinez-Gil L,Vera-Velasco NM,Mingarro I

    更新日期:2017-11-14 00:00:00

  • Biarsenical labeling of vesicular stomatitis virus encoding tetracysteine-tagged m protein allows dynamic imaging of m protein and virus uncoating in infected cells.

    abstract::A recombinant vesicular stomatitis virus (VSV-PeGFP-M-MmRFP) encoding enhanced green fluorescent protein fused in frame with P (PeGFP) in place of P and a fusion matrix protein (monomeric red fluorescent protein fused in frame at the carboxy terminus of M [MmRFP]) at the G-L gene junction, in addition to wild-type (wt...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01668-08

    authors: Das SC,Panda D,Nayak D,Pattnaik AK

    更新日期:2009-03-01 00:00:00

  • Herpes simplex virus inhibits apoptosis through the action of two genes, Us5 and Us3.

    abstract::Apoptosis of virus-infected cells occurs either as a direct response to viral infection or upon recognition of infection by the host immune response. Apoptosis reduces production of new virus from these cells, and therefore viruses have evolved inhibitory mechanisms. We previously showed that laboratory strains of her...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.11.8950-8957.1999

    authors: Jerome KR,Fox R,Chen Z,Sears AE,Lee Hy,Corey L

    更新日期:1999-11-01 00:00:00

  • Arrangement of late RNAs transcribed from a 7.1-kilobase EcoRI vaccinia virus DNA fragment.

    abstract::Three late transcripts have been mapped to the vaccinia virus 7.1-kilobase (kb) EcoRI F fragment, which is located approximately 85 kb from the left end of the viral genome. Hybrid selection and translation of RNA have demonstrated that these mRNAs encode three polypeptides with apparent molecular weights of 32,000 (3...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.49.2.510-520.1984

    authors: Mahr A,Roberts BE

    更新日期:1984-02-01 00:00:00

  • bcl-2 alters influenza virus yield, spread, and hemagglutinin glycosylation.

    abstract::We previously demonstrated that expression of bcl-2 in Madin-Darby canine kidney (MDCK) cells blocks influenza virus-induced apoptosis and DNA fragmentation. We show here that expression of bcl-2 also reduces the level of infectious virus production and the spread of virus in MDCK cell cultures infected at a low multi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.1.663-666.1996

    authors: Olsen CW,Kehren JC,Dybdahl-Sissoko NR,Hinshaw VS

    更新日期:1996-01-01 00:00:00

  • hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain.

    abstract::The major inducible 70-kDa heat shock protein (hsp70) is host protective in a mouse model of measles virus (MeV) brain infection. Transgenic constitutive expression of hsp70 in neurons, the primary target of MeV infection, abrogates neurovirulence in neonatal H-2(d) congenic C57BL/6 mice. A significant level of protec...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02710-12

    authors: Kim MY,Shu Y,Carsillo T,Zhang J,Yu L,Peterson C,Longhi S,Girod S,Niewiesk S,Oglesbee M

    更新日期:2013-01-01 00:00:00

  • Similar patterns of infection with bovine foamy virus in experimentally inoculated calves and sheep.

    abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02447-12

    authors: Materniak M,Hechler T,Löchelt M,Kuzmak J

    更新日期:2013-03-01 00:00:00

  • Host Determinants of Prion Strain Diversity Independent of Prion Protein Genotype.

    abstract:UNLABELLED:Phenotypic diversity in prion diseases can be specified by prion strains in which biological traits are propagated through an epigenetic mechanism mediated by distinct PrP(Sc) conformations. We investigated the role of host-dependent factors on phenotypic diversity of chronic wasting disease (CWD) in differe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01586-15

    authors: Crowell J,Hughson A,Caughey B,Bessen RA

    更新日期:2015-10-01 00:00:00

  • A lysine-to-arginine change found in natural alleles of the human T-cell lymphotropic/leukemia virus type 1 p12(I) protein greatly influences its stability.

    abstract::The HTLV-1 singly spliced open reading frame I protein, p12(I), is highly unstable and appears to be necessary for persistent infection in rabbits. Here we demonstrate that p12(I) forms dimers through two putative leucine zipper domains and that its stability is augmented by specific proteasome inhibitors. p12(I) is u...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.8.6460-6467.1999

    authors: Trovato R,Mulloy JC,Johnson JM,Takemoto S,de Oliveira MP,Franchini G

    更新日期:1999-08-01 00:00:00

  • Transcription factor PRDII-BF1 activates human immunodeficiency virus type 1 gene expression.

    abstract::Gene expression of human immunodeficiency virus (HIV) is modulated by both cellular transcription factors, which bind to cis-acting regulatory elements in the HIV-1 long terminal repeat (LTR) and the viral transactivator, tat. The enhancer element in the HIV-1 LTR which extends from -103 to -82 is critical for gene ex...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.2.1002-1009.1994

    authors: Seeler JS,Muchardt C,Suessle A,Gaynor RB

    更新日期:1994-02-01 00:00:00

  • Human cytomegalovirus infection alters the expression of cellular microRNA species that affect its replication.

    abstract::The human genome encodes over 500 microRNAs (miRNAs), small RNAs (19 to 26 nucleotides [nt]) that regulate the expressions of diverse cellular genes. Many cellular processes are altered through a variety of mechanisms by human cytomegalovirus (HCMV) infection. We asked whether HCMV infection leads to changes in the ex...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00961-08

    authors: Wang FZ,Weber F,Croce C,Liu CG,Liao X,Pellett PE

    更新日期:2008-09-01 00:00:00

  • Amino acid 226 in the hemagglutinin of H9N2 influenza viruses determines cell tropism and replication in human airway epithelial cells.

    abstract::Influenza A viruses of the H9N2 subtype are endemic in poultry in many Eurasian countries and have occasionally caused clinical respiratory diseases in humans. While some avian H9N2 viruses have glutamine (Q) at amino acid position 226 of the hemagglutinin (HA) receptor-binding site, an increasing number of isolates h...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02827-06

    authors: Wan H,Perez DR

    更新日期:2007-05-01 00:00:00