Abstract:
:RIG-I is a major cytoplasmic sensor of viral pathogen-associated molecular pattern (PAMP) RNA and induces type I interferon (IFN) production upon viral infection. A double-stranded RNA (dsRNA)-binding protein, PACT, plays an important role in potentiating RIG-I function. We have shown previously that arenaviral nucleoproteins (NPs) suppress type I IFN production via their RNase activity to degrade PAMP RNA. We report here that NPs of arenaviruses block the PACT-induced enhancement of RIG-I function to mediate type I IFN production and that this inhibition is dependent on the RNase function of NPs, which is different from that of a known mechanism of other viral proteins to abolish the interaction between PACT and RIG-I. To understand the biological roles of PACT and RIG-I in authentic arenavirus infection, we analyze growth kinetics of recombinant Pichinde virus (PICV), a prototypical arenavirus, in RIG-I knockout (KO) and PACT KO mouse embryonic fibroblast (MEF) cells. Wild-type (WT) PICV grew at higher titers in both KO MEF lines than in normal MEFs, suggesting the important roles of these cellular proteins in restricting virus replication. PICV carrying the NP RNase catalytically inactive mutation could not grow in normal MEFs but could replicate to some extent in both KO MEF lines. The level of virus growth was inversely correlated with the amount of type I IFNs produced. These results suggest that PACT plays an important role in potentiating RIG-I function to produce type I IFNs in order to restrict arenavirus replication and that viral NP RNase activity is essential for optimal viral replication by suppressing PACT-induced RIG-I activation.IMPORTANCE We report here a new role of the nucleoproteins of arenaviruses that can block type I IFN production via their specific inhibition of the cellular protein sensors of virus infection (RIG-I and PACT). Our results suggest that PACT plays an important role in potentiating RIG-I function to produce type I IFNs in order to restrict arenavirus replication. This new knowledge can be exploited for the development of novel antiviral treatments and/or vaccines against some arenaviruses that can cause severe and lethal hemorrhagic fever diseases in humans.
journal_name
J Viroljournal_title
Journal of virologyauthors
Shao J,Huang Q,Liu X,Di D,Liang Y,Ly Hdoi
10.1128/JVI.00482-18subject
Has Abstractpub_date
2018-06-13 00:00:00issue
13eissn
0022-538Xissn
1098-5514pii
JVI.00482-18journal_volume
92pub_type
杂志文章abstract::The human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates the HTLV-1 long terminal repeat and key regulatory proteins involved in inflammation, activation, and proliferation and may induce cell transformation. Tax is also the immunodominant target antigen for cytotoxic T cells in HTLV-1 infection. We found...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.11106-11115.2001
更新日期:2001-11-01 00:00:00
abstract::During ϕX174 morphogenesis, 240 copies of the external scaffolding protein D organize 12 pentameric assembly intermediates into procapsids, a reaction reconstituted in vitro In previous studies, ϕX174 strains resistant to exogenously expressed dominant lethal D genes were experimentally evolved. Resistance was achieve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01878-16
更新日期:2016-12-16 00:00:00
abstract::Ebola virus is the etiologic agent of a lethal hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Previous studies with Zaire Ebola virus (ZEBOV), mouse-adapted virus (MA-ZEBOV), and mutant viruses (ZEBOV-NP(ma), ZEBOV-VP24(ma), and ZEBOV-NP/VP24(ma)) allowed us to identify the mutati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00659-11
更新日期:2011-09-01 00:00:00
abstract::The roles of the capsid protein (CP) and the CP coding sequence of tobacco etch potyvirus (TEV) in genome amplification were analyzed. A series of frameshift-stop codon mutations that interrupted translation of the CP coding sequence at various positions were introduced into the TEV genome. A series of 3' deletion mut...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4370-4379.1996
更新日期:1996-07-01 00:00:00
abstract::A unique feature of the large hepadnavirus envelope protein (L) is its mixed transmembrane topology, resulting from partial posttranslational translocation of the pre-S domain. Using protease protection analysis, we demonstrate for duck hepatitis B virus an essential role for the small envelope protein (S) in this pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2455-2458.2000
更新日期:2000-03-01 00:00:00
abstract::Adenovirus infections cause significant morbidity and mortality in immunocompromised patients, yet little is known about the immune response to adenovirus infections. We established a system for the generation of a cytotoxic immune response to adenovirus in vitro. Cytotoxic T cells (CTLs) were derived from normal dono...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6733-6740.1996
更新日期:1996-10-01 00:00:00
abstract:UNLABELLED:Type I IFN signaling, which is initiated through activation of the alpha interferon receptor (IFNAR), regulates the expression of proteins that are crucial contributors to immune responses. Paramyxoviruses, including human metapneumovirus (HMPV), have evolved mechanisms to inhibit IFNAR signaling, but the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03275-14
更新日期:2015-04-01 00:00:00
abstract::Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.585-590.1986
更新日期:1986-02-01 00:00:00
abstract::The development of neoplasia frequently involves inactivation of the p53 and retinoblastoma (Rb) tumor suppressor pathways and disruption of cell cycle checkpoints that monitor the integrity of replication and cell division. The human papillomavirus type 16 (HPV-16) oncoproteins, E6 and E7, have been shown to bind p53...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.975-985.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2765-2771.1997
更新日期:1997-04-01 00:00:00
abstract::Mutants of polyomavirus with altered host specificities were isolated in undifferentiated C2 myoblast cells (L. Ricci, R. Maione, C. Passananti, A. Felsani, and P. Amati, J. Virol. 66:7153-7158, 1992). The mutations responsible for this phenotype belonged to either of the two classes: a large duplication of the enhanc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1196-1199.1994
更新日期:1994-02-01 00:00:00
abstract::The relationship between virus-specific CD8(+)-T-cell responses and viral persistence was studied in mice by using Hantaan virus (HTNV). We first established a simple method for measuring levels of virus-specific CD8(+) T cells by flow cytometry. Next, to produce a mouse model of persistent HTNV infection, newborn mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8408-8417.2003
更新日期:2003-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.120-126.1988
更新日期:1988-01-01 00:00:00
abstract::Viruses in the family Coronaviridae, within the order Nidovirales, are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans. These viruses encode conserved replicase and structural proteins as well as more diverse accessory proteins, encoded in the 3' ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02201-16
更新日期:2017-02-14 00:00:00
abstract::Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.3512-3526.1994
更新日期:1994-06-01 00:00:00
abstract::Herpesviruses use a cascade of interactions with different cell surface molecules to gain entry into cells. In many cases, this involves binding to abundant glycosaminoglycans or integrins followed by interactions with more limited cell surface proteins, leading to fusion with cellular membranes. Human cytomegalovirus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01623-08
更新日期:2008-12-01 00:00:00
abstract::Vaccine-induced memory T cells localized at mucosal sites can provide rapid protection from viral infection. All-trans-retinoic acid (ATRA) has been shown to act physiologically to induce the expression of gut-homing receptors on lymphocytes. We tested whether the administration of exogenous ATRA during a systemic vac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00781-11
更新日期:2011-08-01 00:00:00
abstract::Major histocompatibility complex E (MHC-E) is a highly conserved nonclassical MHC-Ib molecule that tightly binds peptides derived from leader sequences of classical MHC-Ia molecules for presentation to natural killer cells. However, MHC-E also binds diverse foreign and neoplastic self-peptide antigens for presentation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00592-19
更新日期:2019-09-12 00:00:00
abstract::Simian immunodeficiency virus (SIV) of macaques isolate SIVmac239 is highly resistant to neutralization by polyclonal antisera or monoclonal antibodies, a property that it shares with most primary isolates of human immunodeficiency virus type 1 (HIV-1). This resistance is important for the ability of the virus to pers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9993-10003.2003
更新日期:2003-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.5414-5422.2004
更新日期:2004-05-01 00:00:00
abstract::Transfection of an Epstein-Barr virus (EBV)-encoded plasmid containing EBER caused a substantial decrease in the level of plasmid containing EBV in Akata and Mutu Burkitt's lymphoma (BL) lines, but failed to do so in other BL lines. The results suggest that EBER could replace the role of EBV, but other EBV products al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9977-9982.2001
更新日期:2001-10-01 00:00:00
abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
abstract::A panel of murine monoclonal antibodies (MAbs) to the human immunodeficiency virus type 1 trans-activator tat protein were characterized. The anti-tat MAbs were mapped to the different domains of the tat protein by Western blot (immunoblot) and Pepscan analyses. One-half of the MAbs tested mapped to the amino-terminal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.962-965.1990
更新日期:1990-02-01 00:00:00
abstract:UNLABELLED:During the chronic phase of HIV-1 infection, polyfunctional CD8+ T cell responses, which are characterized by a high frequency of cells able to secrete multiple cytokines simultaneously, are associated with lower virus loads and slower disease progression. This relationship may arise for different reasons. P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00647-14
更新日期:2014-09-01 00:00:00
abstract::In various positive-sense single-stranded RNA viruses, a low-fidelity viral RNA-dependent RNA polymerase (RdRp) confers attenuated phenotypes by increasing the mutation frequency. We report a negative-sense single-stranded RNA virus RdRp mutant strain with a mutator phenotype. Based on structural data of RdRp, rationa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00834-19
更新日期:2019-10-29 00:00:00
abstract::A novel species of 30S RNA has been detected in a variety of mouse cell lines. The 30S RNA is specifically packaged by helper-independent type C viruses propagated in such cells. Nucleic acid hybridization detects no homology between the 30SRNA and the genomic RNA of helper-independent mouse type C viruses. The proper...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.115-123.1978
更新日期:1978-01-01 00:00:00
abstract::Each influenza season, a set of wild-type viruses, representing one H1N1, one H3N2, and one to two influenza B isolates, are selected for inclusion in the annual seasonal influenza vaccine. In order to develop broadly reactive subtype-specific influenza vaccines, a methodology called computationally optimized broadly ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01581-17
更新日期:2017-11-30 00:00:00
abstract::A procedure incorporating the use of heparin was developed to purify Herpesvirus ovis. The viral DNA has a buoyant density of 1.706 +/- 0.001 g/cm3, and the sedimentation constant was estimated to be 47.5 +/- 1.5S; from the latter, the molecular weight was calculated as 67.3 +/- 5.4 X 10(6). Estimates of the guanine-p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.705-709.1979
更新日期:1979-12-01 00:00:00
abstract::Binding of human immunodeficiency virus type 1 (HIV-1) to CD4 receptors induces multiple cellular signaling pathways, including the MEK/ERK cascade. While the interaction of X4 HIV-1 with CXCR4 does not seem to activate this pathway, viruses using CCR5 for entry efficiently activate MEK/ERK kinases (W. Popik, J. E. He...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2558-2566.2000
更新日期:2000-03-01 00:00:00
abstract::Phosphorylation of the murine mammary tumor virus (MuMTV) structural proteins was studied in an MuMTV-infected epithelial cell line derived from a BALB/cf C3H mouse mammary tumor. Immunoprecipitation of 32P-labeled cell extracts with monospecific anti-p27 serum revealed that phosphorylation occurred at the stage of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.241-247.1979
更新日期:1979-04-01 00:00:00