Abstract:
:The human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates the HTLV-1 long terminal repeat and key regulatory proteins involved in inflammation, activation, and proliferation and may induce cell transformation. Tax is also the immunodominant target antigen for cytotoxic T cells in HTLV-1 infection. We found that Tax bound to assembled nuclear proteasomes, but Tax could not be detected in the cytoplasm. Confocal microscopy revealed a partial colocalization of Tax with nuclear proteasomes. As Tax translocated into the nucleus very quickly after synthesis, this process probably takes place prior to and independent of proteasome association. Tax mutants revealed that both the Tax N and C termini play a role in proteasome binding. We also found that proteasomes from Tax-transfected cells had enhanced proteolytic activity on prototypic peptide substrates. This effect was not due to the induction of the LMP2 and LMP7 proteasome subunits. Furthermore, Tax appeared to be a long-lived protein, with a half-life of around 15 h. These data suggest that the association of Tax with the proteasome and the enhanced proteolytic activity do not target Tax for rapid degradation and may not determine its immunodominance.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hemelaar J,Bex F,Booth B,Cerundolo V,McMichael A,Daenke Sdoi
10.1128/JVI.75.22.11106-11115.2001subject
Has Abstractpub_date
2001-11-01 00:00:00pages
11106-15issue
22eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
杂志文章abstract::Interleukin-1 receptor antagonist (IL-1ra) gene polymorphisms in 83 human immunodeficiency virus (HIV)-seropositive women were evaluated. Fourteen of the subjects (16.9%) were homozygous for IL-1ra allele 2 (IL-1RN*2). These women had a lower median level of HIV RNA than did women homozygous for allele 1 (IL-1RN*1) (P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
abstract::Bluetongue virus (BTV), an insect-vectored emerging pathogen of both wild ruminants and livestock, has had a severe economic impact in agriculture in many parts of the world. The investigation of BTV replication and pathogenesis has been hampered by the lack of a reverse genetics system. Recovery of infectious BTV is ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-08
更新日期:2008-09-01 00:00:00
abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1134-1142.1974
更新日期:1974-05-01 00:00:00
abstract::Wild-type herpes simplex virus 1 (HSV-1) multiplies, spreads, and rapidly destroys cells of the murine central nervous system (CNS). In contrast, mutants lacking both copies of the gamma(1)34.5- gene have been shown to be virtually lacking in virulence even after direct inoculation of high-titered virus into the CNS o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5560-5569.1997
更新日期:1997-07-01 00:00:00
abstract::Hepadnaviruses contain a relaxed circular DNA genome (RC-DNA) with discontinuities in both strands. Upon infectious entry into a host cell, this genome is converted into a covalently closed superhelical form (CCC-DNA), which later serves as the template for transcription. Here we examined whether the viral polymerase ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4867-4874.1993
更新日期:1993-08-01 00:00:00
abstract::The influenza A virus NS1 protein contains a conserved 4-amino-acid-residue PDZ-ligand binding motif (PBM) at the carboxyl terminus that can function as a virulence determinant by targeting cellular PDZ proteins. The NS1 proteins from avian and human viral isolates have consensus PBM sequences ESEV and RSKV, respectiv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05070-11
更新日期:2011-10-01 00:00:00
abstract::Influenza pandemic preparedness has focused on influenza virus H5 and H7 subtypes. However, it is not possible to predict with certainty which subtype of avian influenza virus will cause the next pandemic, and it is prudent to include other avian influenza virus subtypes in pandemic preparedness efforts. An H6 influen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01206-08
更新日期:2008-11-01 00:00:00
abstract::Only a few archaeal viruses have been subjected to detailed structural analyses. Major obstacles have been the extreme conditions such as high salinity or temperature needed for the propagation of these viruses. In addition, unusual morphotypes of many archaeal viruses have made it difficult to obtain further informat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01347-09
更新日期:2010-01-01 00:00:00
abstract::Infection of monkey cells with human adenovirus (Ad) is abortive, but the infection can be enhanced by coinfecting with simian virus 40 (SV40). However, in the coinfected monkey cells, Ad interferes strongly with SV40 DNA biosynthesis. This interference was found to be a reproducible, delicately controlled phenomenon ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.275-287.1978
更新日期:1978-08-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) subtype C viruses have been found to almost exclusively use the chemokine receptor CCR5 as a coreceptor for entry, even in patients with advanced AIDS. We have characterized subtype C virus isolates from 28 patients from Harare, Zimbabwe, 20 of whom were receiving antiretrov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7682-7688.2003
更新日期:2003-07-01 00:00:00
abstract::Misfolded alpha-synuclein (αS) may exhibit a number of characteristics similar to those of the prion protein, including the apparent ability to spread along neuroanatomical connections. The demonstration for this mechanism of spread is largely based on the intracerebral injections of preaggregated αS seeds in mice, in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00586-18
更新日期:2018-08-29 00:00:00
abstract::In polarized epithelial cells, the vesicular stomatitis virus glycoprotein is segregated to the basolateral plasma membrane, where budding of the virus takes place. We have generated recombinant viruses expressing mutant glycoproteins without the basolateral-membrane-targeting signal in the cytoplasmic domain. Though ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4103-4107.2002
更新日期:2002-04-01 00:00:00
abstract::The Ad2+ND4 virus is an adenovirus type 2 (Ad2)-simian virus 40 (SV40) recombination. The Ad2 genome of this recombinant has a rearrangement within early region 3; Ad2 DNA sequences between map positions 81.3 and 85.5 have been deleted, and the SV40 DNA sequences between map positions 0.11 and 0.626 have been inserted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.344-353.1983
更新日期:1983-08-01 00:00:00
abstract::Therapeutic vaccination has the potential to boost immune responses and enhance viral control during chronic infections. However, many therapeutic vaccination approaches have fallen short of expectations, and effective boosting of antiviral T-cell responses is not always observed. To examine these issues, we studied t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.14.8960-8968.2005
更新日期:2005-07-01 00:00:00
abstract::In most human immunodeficiency virus type 1 (HIV-1)-infected individuals who achieve viral loads of <50 copies/ml during highly active antiretroviral therapy (HAART), low levels of plasma virus remain detectable for years by ultrasensitive methods. The relative contributions of ongoing virus replication and virus prod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00570-09
更新日期:2009-10-01 00:00:00
abstract:UNLABELLED:Intron-containing mRNAs are subject to restricted nuclear export in higher eukaryotes. Retroviral replication requires the nucleocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretroviruses are poorly characterized. Here, we report the involvement of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03584-13
更新日期:2014-04-01 00:00:00
abstract::We determined the capacity of microcapsules formed by the combination of sodium alginate, an aqueous anionic polymer, and spermine hydrochloride, an aqueous cationic amine, to enhance protection against rotavirus challenge in mice. Adult BALB/c mice were orally inoculated with either free or microencapsulated rotaviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3859-3862.1998
更新日期:1998-05-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen associated with chronic liver disease. Recently, based on a genotype 2a isolate, tissue culture systems supporting complete replication and infectious virus production have been developed. In this study, we used cell culture-produced infectious HCV to analyze the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1734-1741.2006
更新日期:2006-02-01 00:00:00
abstract::Heterosubtypic immunity (HSI) is defined as cross-protection to infection with an influenza A virus serotype other than the one used for primary infection. Although HSI has been thought to be mediated by serotype cross-reactive cytotoxic T lymphocytes (CTL) that recognize conserved epitopes of structural proteins, rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00751-07
更新日期:2007-09-01 00:00:00
abstract::The mechanism of heparan sulfate (HS)-mediated human immunodeficiency virus type 1 (HIV-1) binding to and infection of T cells was investigated with a clone (H9h) of the T-cell line H9 selected on the basis of its high level of cell surface CD4 expression. Semiquantitative PCR analysis revealed that enzymatic removal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2233-2239.1995
更新日期:1995-04-01 00:00:00
abstract::Because immunologic classification of human immunodeficiency virus type 1 (HIV) might be more relevant than genotypic classification for designing polyvalent vaccines, studies were undertaken to determine whether immunologically defined groups of HIV ("immunotypes") could be identified. For these experiments, the V3 r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4042-4051.1999
更新日期:1999-05-01 00:00:00
abstract::The recent advent of genome sequences as the only source available to classify many newly discovered viruses challenges the development of virus taxonomy by expert virologists who traditionally rely on extensive virus characterization. In this proof-of-principle study, we address this issue by presenting a computation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07173-11
更新日期:2012-04-01 00:00:00
abstract::Among prion infections, two scenarios of prion spread are generally observed: (i) early lymphoid tissue replication or (ii) direct neuroinvasion without substantial antecedent lymphoid amplification. In nature, cervids are infected with chronic wasting disease (CWD) prions by oral and nasal mucosal exposure, and studi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-17
更新日期:2017-04-28 00:00:00
abstract:UNLABELLED:The HIV-1 envelope protein (Env) is heavily glycosylated, with approximately 50% of the Env molecular mass being contributed by N-glycans. HIV-1 Env N-glycans shield the protein backbone and have been shown to play key roles in determining Env structure, surface exposure, and, consequently, antigenicity, inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02164-14
更新日期:2014-12-01 00:00:00
abstract::Several point mutations in the simian virus 40 (SV40) small-t antigen have been analyzed for their effects on protein stability, transformation, transactivation, and binding of two cellular proteins. All mutations which affected cysteine residues in two cysteine clusters produced highly unstable small-t antigens. Four...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2895-2900.1990
更新日期:1990-06-01 00:00:00
abstract::Live attenuated RNA viruses make highly efficient vaccines. Among them, measles virus (MV) vaccine has been given to a very large number of children and has been shown to be highly efficacious and safe. Therefore, this vaccine might be a very promising vector to immunize children against both measles and other infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11546-11554.2003
更新日期:2003-11-01 00:00:00
abstract::It is generally accepted that the fitness cost of resistance mutations plays a role in the persistence of transmitted drug-resistant human immunodeficiency virus type 1 and that mutations that confer a high fitness cost are less able to persist in the absence of drug pressure. Here, we show that the fitness cost of re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02712-06
更新日期:2007-03-01 00:00:00
abstract::The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-05
更新日期:2006-07-01 00:00:00
abstract::Several viral determinants were shown to play a role in the ability of human immunodeficiency virus type 1 (HIV-1) to infect nondividing cells. In particular, Vpr and Gag matrix (MA) were recognized to be involved in the nuclear transport of the viral preintegration complex. The goal of the present study was to evalua...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4104-4115.1998
更新日期:1998-05-01 00:00:00
abstract::In order to study retroviral variation, selection, and viral correlates of in vivo pathogenicity, we documented the evolution of feline leukemia virus (FeLV) variants in cats that died with thymic lymphoma after infection with molecularly cloned subgroup A FeLV. Using genomic DNA from cat necropsy samples, we employed...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2458-2467.1994
更新日期:1994-04-01 00:00:00