Abstract:
:The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide sets from heterologous subtype A, B, and C viruses. The CON6-derived vaccine was immunogenic and induced a greater number of T-cell epitope responses than any single wild-type subtype A, B, and C env immunogen and similar T-cell responses to a polyvalent vaccine. The responses were comparable to within-clade responses but significantly more than between-clade responses. The magnitude of the T-cell responses induced by CON6 (measured by individual epitope peptides) was also greater than the magnitude of responses induced by individual wild-type env immunogens. Though the limited major histocompatibility complex repertoire in inbred mice does not necessarily predict responses in nonhuman primates and humans, these results suggest that synthetic centralized env immunogens represent a promising approach for HIV-1 vaccine design that merits further characterization.
journal_name
J Viroljournal_title
Journal of virologyauthors
Weaver EA,Lu Z,Camacho ZT,Moukdar F,Liao HX,Ma BJ,Muldoon M,Theiler J,Nabel GJ,Letvin NL,Korber BT,Hahn BH,Haynes BF,Gao Fdoi
10.1128/JVI.02484-05subject
Has Abstractpub_date
2006-07-01 00:00:00pages
6745-56issue
14eissn
0022-538Xissn
1098-5514pii
80/14/6745journal_volume
80pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.61.7.2311-2315.1987
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9317-9321.2000
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pub_type: 杂志文章
doi:10.1128/JVI.72.11.8765-8771.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.1.307-311.1985
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00