Abstract:
:Tegument is a unique structure of herpesvirus, which surrounds the capsid and interacts with the envelope. Morphogenesis of gammaherpesvirus is poorly understood due to lack of efficient lytic replication for Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8, which are etiologically associated with several types of human malignancies. Murine gammaherpesvirus 68 (MHV-68) is genetically related to the human gammaherpesviruses and presents an excellent model for studying de novo lytic replication of gammaherpesviruses. MHV-68 open reading frame 33 (ORF33) is conserved among Alpha-, Beta-, and Gammaherpesvirinae subfamilies. However, the specific role of ORF33 in gammaherpesvirus replication has not yet been characterized. We describe here that ORF33 is a true late gene and encodes a tegument protein. By constructing an ORF33-null MHV-68 mutant, we demonstrated that ORF33 is not required for viral DNA replication, early and late gene expression, viral DNA packaging or capsid assembly but is required for virion morphogenesis and egress. Although the ORF33-null virus was deficient in release of infectious virions, partially tegumented capsids produced by the ORF33-null mutant accumulated in the cytoplasm, containing conserved capsid proteins, ORF52 tegument protein, but virtually no ORF45 tegument protein and the 65-kDa glycoprotein B. Finally, we found that the defect of ORF33-null MHV-68 could be rescued by providing ORF33 in trans or in an ORF33-null revertant virus. Taken together, our results indicate that ORF33 is a tegument protein required for viral lytic replication and functions in virion morphogenesis and egress.
journal_name
J Viroljournal_title
Journal of virologyauthors
Guo H,Wang L,Peng L,Zhou ZH,Deng Hdoi
10.1128/JVI.00497-09subject
Has Abstractpub_date
2009-10-01 00:00:00pages
10582-95issue
20eissn
0022-538Xissn
1098-5514pii
JVI.00497-09journal_volume
83pub_type
杂志文章abstract::The Group B Staphylococcus phage P11-M15 is shown to be 51% protein and 49% deoxyribonucleic acid (DNA). The intact virion has a molecular weight of 66.7 x 10(6) daltons. The purified viral DNA has a molecular weight of 32.7 x 10(6) daltons. The intact virion is shown to be composed of a polyhedral head which is attac...
journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.6233-6236.1992
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4867-4874.1993
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6585-6594.2004
更新日期:2004-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1986-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1977-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.591-597.1984
更新日期:1984-11-01 00:00:00