Abstract:
:Differentiating infected from vaccinated animals (DIVA) strategies have been central enabling techniques in several successful viral disease elimination programs. However, owing to their long and uncertain development process, no DIVA-compatible vaccines are available for many important diseases. We report herein a new DIVA strategy based on hybrid protein-peptide microarrays which can theoretically work with any vaccine. Leading from our findings from peste des petits ruminants (PPR) virus, we found 4 epitope-containing short peptides (ECSPs) which have distinct IgG serodynamics: anti-ECSP IgGs only exist for 10 to 60 days postvaccination (dpv), while anti-protein IgGs remained at high levels for >1,000 dpv. These data enabled the design of a DIVA diagnostic microarray containing 4 ECSPs and 3 proteins, which, unlike competitive enzyme-linked immunosorbent assay (cELISA) and virus neutralization tests (VNTs), enables ongoing monitoring of serological differences between vaccinated individuals and individuals exposed to the pathogen. For 25 goats after 60 dpv, 13 were detected with positive anti-ECSP IgGs, indicating recent infections in vaccinated goat herds. These DIVA diagnostic microarrays will almost certainly facilitate eradication programs for (re)emerging pathogens and zoonoses.IMPORTANCE Outbreaks of infectious diseases caused by viruses, such as pseudorabies (PR), foot-and-mouth disease (FMD), and PPR viruses, led to economic losses reaching billions of dollars. Both PR and FMD were eliminated in several countries via large-scale vaccination programs using DIVA-compatible vaccines, which lack the gE protein and nonstructural proteins, respectively. However, there are still extensive challenges facing the development and deployment of DIVA-compatible vaccines because they are time-consuming and full of uncertainty. Further, the negative marker strategy used for DIVA-compatible vaccines is no longer functional for live-attenuated vaccines. To avoid these disadvantageous scenarios, a new strategy is desired. Here, we made the exciting discovery that different IgG serodynamics can be monitored when using protein-based assays versus arrays comprising ECSPs. This DIVA microarray strategy should, in theory, work for any vaccine.
journal_name
J Viroljournal_title
Journal of virologyauthors
Xue Q,Xu H,Liu H,Pan J,Yang J,Sun M,Chen Y,Xu W,Cai X,Ma Hdoi
10.1128/JVI.01573-19subject
Has Abstractpub_date
2020-02-28 00:00:00issue
6eissn
0022-538Xissn
1098-5514pii
JVI.01573-19journal_volume
94pub_type
杂志文章abstract::The genus β human papillomavirus (HPV) type 8 is associated with nonmelanoma skin cancer in patients with epidermodysplasia verruciformis, and evidence for its protumorigenic potential in the general population increases. To date, strategies to suppress genus β HPV infections are limited. Interferon regulatory factors...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00998-10
更新日期:2011-01-01 00:00:00
abstract::Although adeno-associated virus type 2 (AAV) has gained attention as a potentially useful vector for human gene therapy, the transduction efficiencies of AAV vectors vary greatly in different cells and tissues in vitro and in vivo. We have documented that a cellular tyrosine phosphoprotein, designated the single-stran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.8968-8976.2001
更新日期:2001-10-01 00:00:00
abstract::Highly pathogenic influenza A viruses (IAV) from avian hosts were first reported to directly infect humans 20 years ago. However, such infections are rare events, and our understanding of factors promoting or restricting zoonotic transmission is still limited. One accessory protein of IAV, PB1-F2, was associated with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00425-18
更新日期:2018-05-14 00:00:00
abstract::Retroviruses have been isolated from the tissues of human leukemia patients. Previous studies have shown that these isolates share some antigenic determinants with the family of viruses isolated from the woolly monkey and gibbon ape and that they exhibit partial nuclei acid homology with this same group of viruses. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.2.390-401.1980
更新日期:1980-05-01 00:00:00
abstract::Among the members of the paramyxovirus family, the transcription process and the components involved have been studied under in vitro conditions thus far. Here, we reexamined the function of the viral RNA-dependent RNA polymerase through infection studies with Sendai virus (SeV) N and P deletion (Delta) mutants. To el...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00914-07
更新日期:2007-12-01 00:00:00
abstract::A phylogenetic analysis of 52 published and 37 new nucleoprotein (NP) gene sequences addressed the evolution and origin of human and swine influenza A viruses. H1N1 human and classical swine viruses (i.e., those related to Swine/Iowa/15/30) share a single common ancestor, which was estimated to have occurred in 1912 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3704-3714.1991
更新日期:1991-07-01 00:00:00
abstract::Four genetic loci were tested for linkage with loci that control genetic resistance to lethal ectromelia virus infection in mice. Three of the loci were selected because of concordance with genotypes assigned to recombinant inbred (RI) strains of mice derived from resistant C57BL/6 and susceptible DBA/2 (BXD) mice on ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1946-1951.1991
更新日期:1991-04-01 00:00:00
abstract::Clones of virogenic simian virus 40 (SV40)-transformed hamster kidney cells were exposed to medium deficient in the essential amino acids leucine, arginine, or methionine. Infectious virus was induced after deprivation periods of from 24 to 32 hr. The highest yields of infectious SV40 were obtained from cultures depri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.3.448-453.1972
更新日期:1972-03-01 00:00:00
abstract::Paired immunoglobulin (Ig)-like type 2 receptor alpha (PILRalpha) and PILRbeta are paired receptors that are highly homologous to each other. When engaged by ligand, PILRalpha is inhibitory whereas PILRbeta is activating. PILRalpha is a newly identified herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) receptor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01039-10
更新日期:2010-09-01 00:00:00
abstract::Mouse mammary tumor virus (MTV) was isolated from the milk of RIII mice by density-gradient centrifugation in Ficoll. The homogeneity of the preparation was demonstrated in electron micrographs. The nucleic acid was extracted with phenol in the presence of Pronase. Its viral origin was attested by failure of ribo-nucl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.2.230-236.1970
更新日期:1970-02-01 00:00:00
abstract::Interferon (IFN) signal transduction involves interferon regulatory factors (IRF). Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four IRF homologues: viral IRF 1 (vIRF-1) to vIRF-4. Previous functional studies revealed that the first exon of vIRF-2 inhibited alpha/beta interferon (IFN-alpha/beta) signaling. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.6.3092-3097.2006
更新日期:2006-03-01 00:00:00
abstract::Marek's disease virus (MDV) is a herpesvirus of chickens that induces T lymphomas and tumors within 4 to 5 weeks of infection. Although the ability of MDV to induce tumors was demonstrated many years ago and although a number of viral oncogenic proteins have been identified, the mechanism by which the MDV is implicate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5985-5996.2003
更新日期:2003-05-01 00:00:00
abstract:UNLABELLED:Human immunodeficiency virus type 1 (HIV-1) replication in dendritic cells (DCs) is restricted by SAMHD1. This factor is counteracted by the viral protein Vpx; Vpx is found in HIV-2 and simian immunodeficiency virus (SIV) from sooty mangabeys (SIVsm) or from macaques (SIVmac) but is absent from HIV-1. We pre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03057-13
更新日期:2014-05-01 00:00:00
abstract::Generating durable humoral immunity through vaccination depends upon effective interactions of follicular helper T (Tfh) cells with germinal center (GC) B cells. Th1 polarization of Tfh cells is an important process shaping the success of Tfh-GC B cell interactions by influencing costimulatory and cytokine-dependent T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01737-19
更新日期:2020-02-28 00:00:00
abstract::The unexpectedly low efficacy of influenza vaccine during school outbreaks of influenza B virus in the spring of 1987 in Japan was probably attributable to a poor antibody response of vaccinees to the epidemic viruses. An antigenic analysis of the causative B viruses isolated in 1987 and 1988 showed much variation in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
更新日期:1990-06-01 00:00:00
abstract::We observed two patterns of chemokine expression in the lungs of mice infected with murine gammaherpesvirus 68: peaks of chemokine expression correlated with or occurred after the peak of viral gene expression. Chemokine expression remained elevated through 29 days postinfection. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10518-10523.2002
更新日期:2002-10-01 00:00:00
abstract::Like other herpesviruses, Kaposi's sarcoma-associated herpesvirus (KSHV, also designated human herpesvirus 8) can establish a latent infection in the infected host. During latency a small number of genes are expressed. One of those genes encodes latency-associated nuclear antigen (LANA), which is constitutively expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6585-6594.2004
更新日期:2004-06-01 00:00:00
abstract::The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named Botrytis porri RNA virus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri. The BpRV1 genome comprises t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00292-12
更新日期:2012-06-01 00:00:00
abstract::The Epstein-Barr virus (EBV) immediate-early protein BZLF1 mediates the switch between the latent and lytic forms of EBV infection and has been previously shown to induce a G(1)/S block in cell cycle progression in some cell types. To examine the effect of BZLF1 on cellular gene expression, we performed microarray ana...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12543-12552.2002
更新日期:2002-12-01 00:00:00
abstract::To investigate the genetic stability of vaccinia virus DNA, we have tested whether alterations occurred in the polypeptide composition of this complex virus during persistent infections. We found that variants isolated at various passages in Friend erythroleukemia cells persistently infected with vaccinia virus contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2642-2647.1987
更新日期:1987-08-01 00:00:00
abstract::A characterization of the antigenic determinants (epitopes) of the glycoprotein (G) of infectious hematopoietic necrosis virus was made by expressing different regions of the G gene in Escherichia coli. A cDNA copy of the G gene was divided into four fragments by TaqI digestion, and the fragments were subcloned into p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1611-1615.1991
更新日期:1991-03-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::Active proteinase 3C of hepatitis A virus (HAV) was expressed in bacteria either as a mature enzyme or as a protein fused to the entire polymerase 3D or to a part of it, and their identities were shown by immunoblot analysis. Intermolecular cleavage activity was demonstrated by incubating in vitro-translated and radio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6794-6796.1992
更新日期:1992-11-01 00:00:00
abstract::Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the Podoviridae family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02727-12
更新日期:2012-12-01 00:00:00
abstract::Reassortant viruses which possessed the hemagglutinin and neuraminidase genes of wild-type human influenza A viruses and the remaining six RNA segments (internal genes) of the avian A/Pintail/Alberta/119/79 (H4N6) virus were previously found to be attenuated in humans. To study the genetic basis of this attenuation, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2857-2863.1987
更新日期:1987-09-01 00:00:00
abstract::Papillomavirus disease poses a special challenge to people with compromised immune systems. Appropriate models to study infections in these individuals are lacking. We report here the development of a model that will help to address these deficiencies. The MmuPV1 genome was synthesized and used successfully to produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00777-13
更新日期:2013-08-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) establishes a lifelong latent infection in host peripheral neurons, including the neurons of the trigeminal ganglia (TG). HSV-1 can reactivate from neurons to cause recurrent infection. During latency, the insulator protein CTCF occupies DNA binding sites on the HSV-1 genome, and these s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00173-18
更新日期:2018-05-14 00:00:00
abstract::Human metapneumovirus (HMPV) has recently been identified as a significant cause of serious respiratory tract disease in humans. In particular, the emerging information on the contribution of HMPV to pediatric respiratory tract disease suggests that it will be important to develop a vaccine against this virus for use ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.12877-12887.2004
更新日期:2004-12-01 00:00:00
abstract:UNLABELLED:Human metapneumovirus (HMPV), a recently discovered paramyxovirus, infects nearly 100% of the world population and causes severe respiratory disease in infants, the elderly, and immunocompromised patients. We previously showed that HMPV binds heparan sulfate proteoglycans (HSPGs) and that HMPV binding requir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01362-16
更新日期:2016-09-29 00:00:00
abstract::Human cytomegalovirus (HCMV) infection of monocyte-derived macrophages (MDM) results in delayed and nonlytic productive viral growth. During late stages of replication, infectious virus remains cell associated in cytoplasmic vacuoles. In order to understand HCMV survival and persistence in MDM, we examined mechanisms ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1855-1862.1996
更新日期:1996-03-01 00:00:00