Abstract:
:The S1 gene nucleotide sequences of 10 type 3 (T3) reovirus strains were determined and compared with the T3 prototype Dearing strain in order to study sequence diversity in strains of a single reovirus serotype and to learn more about structure-function relationships of the two S1 translation products, sigma 1 and sigma 1s. Analysis of phylogenetic trees constructed from variation in the sigma 1-encoding S1 nucleotide sequences indicated that there is no pattern of S1 gene relatedness in these strains based on host species, geographic site, or date of isolation. This suggests that reovirus strains are transmitted rapidly between host species and that T3 strains with markedly different S1 sequences circulate simultaneously. Comparison of the deduced sigma 1 amino acid sequences of the 11 T3 strains was notable for the identification of conserved and variable regions of sequence that correlate with the proposed domain organization of sigma 1 (M.L. Nibert, T.S. Dermody, and B. N. Fields, J. Virol. 64:2976-2989, 1990). Repeat patterns of apolar residues thought to be important for sigma 1 structure were conserved in all strains examined. The deduced sigma 1s amino acid sequences of the strains were more heterogeneous than the sigma 1 sequences; however, a cluster of basic residues near the amino terminus of sigma 1s was conserved. This analysis has allowed us to investigate molecular epidemiology of T3 reovirus strains and to identify conserved and variable sequence motifs in the S1 translation products, sigma 1 or sigma 1s.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dermody TS,Nibert ML,Bassel-Duby R,Fields BNdoi
10.1128/JVI.64.10.4842-4850.1990subject
Has Abstractpub_date
1990-10-01 00:00:00pages
4842-50issue
10eissn
0022-538Xissn
1098-5514journal_volume
64pub_type
杂志文章abstract::The second of three short upstream open reading frames (uORF2) in the transcript leader of the human cytomegalovirus gp48 (gpUL4) virion glycoprotein gene inhibits downstream translation approximately 10-fold. Remarkably, this inhibition depends on the amino acid coding information of uORF2. In the current studies we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1030-1036.1995
更新日期:1995-02-01 00:00:00
abstract::The N-terminal domain of the bovine papillomavirus type 1 E2 protein is important for viral DNA replication, for transcriptional transactivation, and for interaction with the E1 protein. To determine which residues of this 200-amino-acid domain are important for these activities, single conservative amino acid substit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.23-29.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01689-10
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8382-8391.2004
更新日期:2004-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.963-970.1981
更新日期:1981-12-01 00:00:00
abstract::The DNA product of the endogenously instructed RNA-dependent DNA polymerase reaction of murine sarcoma virus continued to be synthesized for as long as 64 h in the presence of 0.008% Triton X-100. Higher detergent concentrations and actinomycin D inhibited DNA product synthesis. The DNA product from long-term polymera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.5.1049-1064.1973
更新日期:1973-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3025-3032.1990
更新日期:1990-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01279-19
更新日期:2020-01-06 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01957-19
更新日期:2020-03-31 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.243-248.1978
更新日期:1978-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03565-13
更新日期:2014-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5627-5637.2001
更新日期:2001-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3811-3818.2001
更新日期:2001-04-01 00:00:00
abstract::Recently, we showed that the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both virion types, budded virus (BV) and occlusion-derived virus (ODV). Deletion of the vp80 gene did not affect assembly of nucleocapsids. However, these nucleocapsids were not ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00035-11
更新日期:2011-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.5974-5984.2002
更新日期:2002-06-01 00:00:00
abstract::The immediate-early gene ie2 of murine cytomegalovirus was characterized. The 1.75-kb ie2 transcript is spliced from three exons, of 78, 124, and 1,283 nucleotides, which are separated by introns of 1,245 and 364 nucleotides. An ATG codon located in the third exon leads into an open reading frame of 391 codons. Immedi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1638-1643.1991
更新日期:1991-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02181-17
更新日期:2018-04-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1781-1786.2000
更新日期:2000-02-01 00:00:00
abstract::Autographa californica nuclear polyhedrosis virus (AcMNPV) is a double-stranded DNA virus that expresses several immediate-early genes under the control of different promoters. The expression of one of these transcription units, IE-N, is shown here, by a transient expression assay, to be regulated by both cis- and tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.945-951.1991
更新日期:1991-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.528-533.1995
更新日期:1995-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02201-08
更新日期:2009-04-01 00:00:00
abstract::Recently, novel H7N9 influenza viruses have caused an unprecedented outbreak in humans. Pigs are an important intermediate host for influenza; thus, we assessed the replication ability of three human H7N9 viruses (A/Anhui/1/2013, A/Shanghai/1/2013, A/Shanghai/2/2013) in swine tissue explants. All viruses tested replic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02499-13
更新日期:2013-11-01 00:00:00
abstract::Retroviral DNA integration requires the activity of at least one viral protein, the integrase (IN) protein. We cloned and expressed the integrase gene of feline immunodeficiency virus (FIV) in Escherichia coli as a fusion to the malE gene and purified the IN fusion protein by affinity chromatography. The protein is ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1468-1474.1994
更新日期:1994-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.686-691.1984
更新日期:1984-03-01 00:00:00
abstract::The polyomavirus proteins VP1, VP2, and VP3 move from their cytoplasmic site of synthesis into the nucleus, where virus assembly occurs. To identify cellular or viral components which might control this process, we determined the distribution of VP1, VP2, and VP3 in a soluble fraction, a cytoplasmic cytoskeleton fract...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.516-525.1987
更新日期:1987-02-01 00:00:00
abstract::Poliovirus-specific T lymphocytes were isolated from virus-immunized mice of different H-2 haplotypes. Immunological characterization of this population indicates that the effector population involved in the observed poliovirus-specific proliferative response was that of CD4-positive T-helper cells. Proliferative resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.3042-3047.1992
更新日期:1992-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2018-09-12 00:00:00
abstract::YY1 is a zinc finger transcription factor which acts as either a repressor or an activator dependent on the promoter context. YY1 is a potent activator of the genuine human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) in HeLa cells, which are known for high-level expression of the HPV-18 early gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.1-12.1995
更新日期:1995-01-01 00:00:00
abstract::Bacteriophage with linear, double-stranded DNA genomes package DNA into preassembled protein shells called procapsids. Located at one vertex in the procapsid is a portal complex composed of a ring of 12 subunits of portal protein. The portal complex serves as a docking site for the DNA packaging enzymes, a conduit for...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
abstract::High-risk human papillomavirus type 16 (HPV-16) and HPV-18 are associated with the majority of human cervical carcinomas, and two viral genes, HPV E6 and E7, are commonly found to be expressed in these cancers. The presence of HPV-16 E7 is sufficient to induce epidermal hyperplasia and epithelial tumors in transgenic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.8.5905-5914.1997
更新日期:1997-08-01 00:00:00