Abstract:
:The genome of lymphocytic choriomeningitis virus (LCMV) consists of two negative-sense single-stranded RNA segments, designated L and S. Both segments contain two viral genes in an ambisense coding strategy, with the genes being separated by an intergenic region (IGR). We have developed a reverse genetic system that allows the investigation of cis-acting signals and trans-acting factors involved in transcription and replication of LCMV. To this end, we constructed an LCMV S minigenome consisting of a negative-sense copy of the chloramphenicol acetyltransferase (CAT) reporter gene flanked upstream by the S 5' untranslated region (UTR) and IGR and downstream by the S 3' UTR. CAT expression was detected in LCMV-infected cells transfected with the minigenome RNA. Intracellular coexpression of the LCMV minigenome and LCMV L and NP proteins supplied from cotransfected plasmids driven by the T7 RNA polymerase provided by the recombinant vaccinia virus vTF7-3 resulted in high levels of CAT activity and synthesis of subgenomic CAT mRNA and antiminigenome RNA species. Thus, L and NP represent the minimal viral trans-acting factors required for efficient RNA synthesis mediated by LCMV polymerase.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lee KJ,Novella IS,Teng MN,Oldstone MB,de La Torre JCdoi
10.1128/jvi.74.8.3470-3477.2000subject
Has Abstractpub_date
2000-04-01 00:00:00pages
3470-7issue
8eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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doi:10.1128/JVI.72.2.1060-1070.1998
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pub_type: 杂志文章
doi:10.1128/JVI.61.4.1244-1247.1987
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doi:10.1128/JVI.71.4.2765-2771.1997
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pub_type: 杂志文章
doi:10.1128/JVI.28.1.28-33.1978
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4485-4494.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.6263-6269.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4856-4866.1993
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2409-2414.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.105-112.1977
更新日期:1977-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.16.8712-8718.2003
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.233-240.1971
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2336-2347.2004
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5703-5710.2001
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doi:10.1128/JVI.00214-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7248-7256.2004
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abstract::A number of full-length cDNA clones of Kunjin virus (KUN) were previously prepared; it was shown that two of them, pAKUN and FLSDX, differed in specific infectivities of corresponding in vitro transcribed RNAs by approximately 100,000-fold (A. A. Khromykh et al., J. Virol. 72:7270-7279, 1998). In this study, we analyz...
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.7900-7908.1994
更新日期:1994-12-01 00:00:00
abstract::Koi herpesvirus (KHV) is the causative agent of a lethal disease in koi and common carp. In the present study, we describe the cloning of the KHV genome as a stable and infectious bacterial artificial chromosome (BAC) clone that can be used to produce KHV recombinant strains. This goal was achieved by the insertion of...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.6070-6075.2003
更新日期:2003-05-01 00:00:00