Abstract:
:Regulation of immediate-early gene expression in human cytomegalovirus is subject to complex controls. The major immediate-early (mIE) gene is regulated by both positive and negative regulatory signals, including autoregulation mediated by a cis-repressive sequence. A second immediate-early gene, the US3 gene, is transcribed with kinetic similar to those of the mIE gene. I have identified an element present in the US3 gene located from -1 to -13 (relative to the start site of transcription) that mediates a decrease in US3 transcription. The US3 element resembles the cis-repressive element of the mIE gene in sequence, position, and function. The common theme of negative regulation of immediate-early genes shortly after infection suggests that a decrease in the level of immediate-early proteins may be critical for viral replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Biegalke BJdoi
10.1128/JVI.69.9.5362-5367.1995subject
Has Abstractpub_date
1995-09-01 00:00:00pages
5362-7issue
9eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::The γ134.5 gene of herpes simplex virus 1 (HSV-1) encodes a virulence factor that promotes viral pathogenesis. Although it perturbs TANK-binding kinase 1 (TBK1) in the complex network of innate immune pathways, the underlying mechanism is obscure. Here we report that HSV-1 γ134.5 targets stimulator of interferon genes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01015-18
更新日期:2018-09-26 00:00:00
abstract::Varicella-zoster virus (VZV), an alphaherpesvirus restricted to humans, infects differentiated cells in vivo, including T lymphocytes, keratinocytes, and neurons, and spreads rapidly in confluent cultured dermal fibroblasts (HFFs). In VZV-infected HFFs, atypical expression of cyclins D3 and B1 occurs along with the in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00153-08
更新日期:2008-12-01 00:00:00
abstract::LA3382 is a temperature-sensitive replication-defective mutant of Rous sarcoma virus that contains four active mutations. In this study we performed experiments to determine the biochemical defect that blocks the synthesis of infections virus late in the replication cycle. At the nonpermissive temperature (41 degrees ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.752-761.1981
更新日期:1981-12-01 00:00:00
abstract::The alphaherpesvirus proteins UL31 and UL34 and their homologues in other herpesvirus subfamilies cooperate at the nuclear membrane in the export of nascent herpesvirus capsids. We studied the respective betaherpesvirus proteins M53 and M50 in mouse cytomegalovirus (MCMV). Recently, we established a random approach to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00681-10
更新日期:2010-09-01 00:00:00
abstract::Influenza A virus matrix protein M1 is involved in multiple stages of the viral infectious cycle. Despite its functional importance, our present understanding of this essential viral protein is limited. The roles of a small subset of specific amino acids have been reported, but a more comprehensive understanding of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00161-19
更新日期:2019-06-14 00:00:00
abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::Storage of the dimeric (alphabeta) form of avian myeloblastosis virus (AMV) DNA polymerase in glycerol resulted in the release of the smaller alpha subunit, as detected by glycerol gradient sedimentation. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of enzyme stored in glycerol showed the conc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.904-910.1976
更新日期:1976-06-01 00:00:00
abstract::Latent HIV proviruses are silenced as the result of deacetylation and methylation of histones located at the viral long terminal repeat (LTR). Inhibition of histone deacetylases (HDACs) leads to the reemergence of HIV-1 from latency, but the contribution of histone lysine methyltransferases (HKMTs) to maintaining HIV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00836-11
更新日期:2011-09-01 00:00:00
abstract::The combination of an amino acid deletion at codon 67 (delta 67) and Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is associated with high-level resistance to multiple RT inhibitors. To determine the relative contributions of the delta 67 and T69G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.10958-10964.2000
更新日期:2000-12-01 00:00:00
abstract::Both exponentially growing and serum-arrested subcloned CV-1 cell cultures were infected with simian virus 40 (SV40). By 24 h after infection 96% of the nuclei of these permissive cells contained SV40 T-antigen. Analysis of the average DNA content per cell at various times after infection indicated that by 24 h most o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.1.76-83.1979
更新日期:1979-04-01 00:00:00
abstract::Heterosubtypic immunity (HSI) is defined as cross-protection to infection with an influenza A virus serotype other than the one used for primary infection. Although HSI has been thought to be mediated by serotype cross-reactive cytotoxic T lymphocytes (CTL) that recognize conserved epitopes of structural proteins, rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00751-07
更新日期:2007-09-01 00:00:00
abstract::The mechanisms involved in the emergence of virulent mammalian viruses were investigated in the adult immunodeficient SCID mouse infected by the attenuated prototype strain of the parvovirus Minute Virus of Mice (MVMp). Cloned MVMp intravenously inoculated in mice consistently evolved during weeks of subclinical infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11280-11290.2005
更新日期:2005-09-01 00:00:00
abstract::Since HIV-1 replication is modulated at multiple stages by host cell factors, identification and characterization of those host cell factors are expected to contribute to the development of novel anti-HIV therapeutics. Previous studies showed that a C-terminally truncated cytosolic form of cleavage and polyadenylation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00124-13
更新日期:2013-07-01 00:00:00
abstract::The host cell-specific factor 1 gene (hcf-1) of the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) is required for the efficient replication and/or stability of reporter plasmids carrying an AcMNPV-derived origin of DNA replication in a cell-specific manner; hcf-1 is required for reporter plasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5123-5130.1996
更新日期:1996-08-01 00:00:00
abstract:UNLABELLED:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retrov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03587-13
更新日期:2014-05-01 00:00:00
abstract::It is shown that the plasmid prophage P1 can be displaced by a superinfecting P1 phage in Escherichia coli recA(P1) lysogens. Six widely separated phage markers were used to distinguish between residual recombination and total substitution. It is further shown that superinfection of recA lysogens can lead to loss of b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.2.277-280.1979
更新日期:1979-08-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) gene was altered so that it encoded a truncated glycoprotein lacking a cytoplasmic domain but retaining 20 of 23 amino acids of the transmembrane domain. No additional amino acid residues were introduced into the glycoprotein encoded by the altered gene. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1753-1761.1988
更新日期:1988-05-01 00:00:00
abstract:UNLABELLED:Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and characterized by a high rate of abortion in ruminants and hemorrhagic fever, encephalitis, or blindness in humans. RVF is caused by Rift Valley fever virus (RVFV; family Bunyaviridae, genus Phlebovirus), which has a tripartite...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00135-15
更新日期:2015-07-01 00:00:00
abstract::We have examined the exposure and conservation of antigenic epitopes on the surface envelope glycoproteins (gp120 and gp41) of 26 intact, native, primary human immunodeficiency virus type 1 (HIV-1) group M virions of clades A to H. For this, 47 monoclonal antibodies (MAbs) derived from HIV-1-infected patients were use...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7096-7107.2000
更新日期:2000-08-01 00:00:00
abstract::RNA packaging signals (psi) from the 5' ends of murine and avian retroviral genomes have previously been shown to direct encapsidation of heterologous mRNA into the retroviral virion. The avian 5' packaging region has now been further characterized, and we have defined a 270-nucleotide sequence, A psi, which is suffic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.178-188.1993
更新日期:1993-01-01 00:00:00
abstract::Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identifie...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00735-09
更新日期:2009-08-01 00:00:00
abstract::The retroviral primary transcription product is a multifunctional RNA that is utilized as pre-mRNA, mRNA, and genomic RNA. The relationship between human immunodeficiency virus type 1 (HIV-1) unspliced transcripts used as mRNA for viral protein synthesis and as virion precursor RNA (vpRNA) for encapsidation remains an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11531-11537.2000
更新日期:2000-12-01 00:00:00
abstract::For paramyxoviruses, entry requires a receptor-binding protein (hemagglutinin-neuraminidase [HN], H, or G) and a fusion protein (F). Like other class I viral fusion proteins, F is expressed as a prefusion metastable protein that undergoes a refolding event to induce fusion. HN binding to its receptor triggers F refold...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01191-09
更新日期:2009-11-01 00:00:00
abstract::Human adenovirus type 12 was propagated on human embryonic kidney cells, and the specific infectivities of intra- and extracellular virus particles were compared between 48 and 104 h after infection. Released virions exhibited a specific infectivity of up to 10 times higher than that of intracellular particles. The in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.1.117-125.1985
更新日期:1985-07-01 00:00:00
abstract::An mRNA-ribonucleoprotein particle (mRNP) was found in vesicular stomatitis virus (VSV)-infected Chinese hamster ovary cells. The particle was present 3 and 4.5 h after infection but was barely discernible at 2 h. The mRNP (buoyant density, 1.56 g/cm3), which cosedimented with viral nucleocapsid in a sucrose density g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.932-938.1982
更新日期:1982-12-01 00:00:00
abstract::In varicella-zoster virus (VZV)-infected primary human brain vascular adventitial fibroblasts (BRAFs), levels of beta interferon (IFN-β,) STAT1, and STAT2 transcripts as well as STAT1 and STAT2 protein were decreased. IFN-α transcript levels were increased but not secreted IFN-α protein levels. Compared to IFN-α-treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01945-14
更新日期:2014-10-01 00:00:00
abstract::The sheep genome harbors approximately 20 endogenous retroviruses (enJSRVs) highly related to the exogenous Jaagsiekte sheep retrovirus (JSRV). One of the enJSRV loci, enJS56A1, acts as a unique restriction factor by blocking JSRV in a transdominant fashion at a late stage of the retroviral cycle. To better understand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01859-06
更新日期:2007-02-01 00:00:00
abstract::The Epstein-Barr virus (EBV) nuclear antigen 3C (EBNA-3C) protein is a transcriptional regulator of viral and cellular genes that is essential for EBV-mediated immortalization of B lymphocytes in vitro. EBNA-3C can inhibit transcription through an association with the cellular DNA-binding protein Jkappa, a function sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5151-5160.2000
更新日期:2000-06-01 00:00:00
abstract::HIV-1 Nef promotes virus spread and disease progression by altering host cell transport and signaling processes through interaction with multiple host cell proteins. The N-terminal region in HIV-1 Nef encompassing residues 12 to 39 has been implicated in many Nef activities, including disruption of CD4 T lymphocyte po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01398-19
更新日期:2019-12-12 00:00:00
abstract::Multimerization of the Hantaan virus nucleocapsid protein (NP) in Hantaan virus-infected Vero E6 cells was observed in a competitive enzyme-linked immunosorbent assay (ELISA). Recombinant and truncated NPs of Hantaan, Seoul, and Dobrava viruses lacking the N-terminal 49 amino acids were also detected as multimers. Alt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.943-952.2003
更新日期:2003-01-01 00:00:00