Abstract:
:Live attenuated human immunodeficiency virus type 1 (HIV-1) vaccines are considered unsafe because faster-replicating pathogenic virus variants may evolve after vaccination. We previously presented a conditional-live HIV-1 variant of which replication can be switched off as an alternative vaccination strategy. To improve the safety of such a vaccine, we constructed a new HIV-1 variant that depends not only on doxycycline for gene expression but also on the T20 peptide for cell entry. Replication of this virus can be limited to the level required to induce the immune system by transient administration of doxycycline and T20. Subsequent withdrawal of these inducers efficiently blocks viral replication and evolution.
journal_name
J Viroljournal_title
Journal of virologyauthors
Das AT,Baldwin CE,Vink M,Berkhout Bdoi
10.1128/JVI.79.6.3855-3858.2005subject
Has Abstractpub_date
2005-03-01 00:00:00pages
3855-8issue
6eissn
0022-538Xissn
1098-5514pii
79/6/3855journal_volume
79pub_type
杂志文章abstract::A large number of polyoma virus-transformed cells of rat, mouse, and hamster origin were examined for presence of T-antigen species. The results showed that all lines of cells contained middle and small T antigens, but not all contained a full-sized large T antigen, in some cell lines large T antigen was absent, where...
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00