Spacers increase the accessibility of peptide ligands linked to the carboxyl terminus of adenovirus minor capsid protein IX.

Abstract:

:The efficiency and specificity of gene transfer with human adenovirus (hAd)-derived gene transfer vectors would be improved if the native viral tropism could be modified. Here, we demonstrate that the minor capsid protein IX (pIX), which is present in 240 copies in the Ad capsid, can be exploited as an anchor for heterologous polypeptides. Protein IX-deleted hAd5 vectors were propagated in hAd5 helper cells expressing pIX variants, with heterologous carboxyl-terminal extensions of up to 113 amino acids in length. The extensions evaluated consist of alpha-helical spacers up to 75 A in length and to which peptide ligands were fused. The pIX variants were efficiently incorporated into the capsids of Ad particles. On intact particles, the MYC-tagged-pIX molecules were readily accessible to anti-MYC antibodies, as demonstrated by electron microscopic analyses of immunogold-labeled virus particles. The labeling efficiency improved with increasing spacer length, suggesting that the spacers lift and expose the ligand at the capsid surface. Furthermore, we found that the addition of an integrin-binding RGD motif to the pIX markedly stimulated the transduction of coxsackievirus group B and hAd receptor-deficient endothelioma cells, demonstrating the utility of pIX modification in gene transfer. Our data demonstrate that the minor capsid protein IX can be used as an anchor for the addition of polypeptide ligands to Ad particles.

journal_name

J Virol

journal_title

Journal of virology

authors

Vellinga J,Rabelink MJ,Cramer SJ,van den Wollenberg DJ,Van der Meulen H,Leppard KN,Fallaux FJ,Hoeben RC

doi

10.1128/jvi.78.7.3470-3479.2004

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

3470-9

issue

7

eissn

0022-538X

issn

1098-5514

journal_volume

78

pub_type

杂志文章
  • 5'-terminal nucleotide sequences of mammalian type C helper viruses are conserved in the genomes of replication-defective mammalian transforming viruses.

    abstract::The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.26.3.570-576.1978

    authors: Tronick SR,Cabradilla CD,Aaronson SA,Haseltine WA

    更新日期:1978-06-01 00:00:00

  • Influenza Virus Neuraminidase Engages CD83 and Promotes Pulmonary Injury.

    abstract::Influenza A viruses cause severe respiratory illnesses in humans and animals. Overreaction of the innate immune response to influenza virus infection results in hypercytokinemia, which is responsible for mortality and morbidity. However, the mechanism by which influenza induces hypercytokinemia is not fully understood...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01753-20

    authors: Ma N,Li X,Jiang H,Dai Y,Xu G,Zhang Z

    更新日期:2021-01-13 00:00:00

  • Characterization of bovine viral diarrhea virus proteins.

    abstract::Virus-specific proteins were examined in cultured cells infected with bovine viral diarrhea virus. By using antisera obtained from virus-infected animals, three major virus-specific polypeptides with molecular weights of 115,000 (115K), 80K, and 55K were observed. Minor proteins of 45,000 and 38,000 daltons were also ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.50.2.666-669.1984

    authors: Purchio AF,Larson R,Collett MS

    更新日期:1984-05-01 00:00:00

  • Inducible MicroRNA-3570 Feedback Inhibits the RIG-I-Dependent Innate Immune Response to Rhabdovirus in Teleost Fish by Targeting MAVS/IPS-1.

    abstract::Effectively recognizing invading viruses and subsequently inducing innate antiviral immunity are essential for host antiviral defense. Although these processes are closely regulated by the host to maintain immune balance, viruses have evolved the ability to downregulate or upregulate these processes for their survival...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01594-17

    authors: Xu T,Chu Q,Cui J,Bi D

    更新日期:2018-01-02 00:00:00

  • Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins.

    abstract::The recent finding that the E1 glycoproteins of murine coronaviruses contain only O-linked oligosaccharides suggested that this unusual modification might be a distinguishing feature of coronaviruses and might play an essential role in the life cycle of this family of viruses. To examine these possibilities, we analyz...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.44.3.804-812.1982

    authors: Stern DF,Sefton BM

    更新日期:1982-12-01 00:00:00

  • Inhibition by interleukin-4 of constitutive beta interferon synthesis in mouse macrophages.

    abstract::We have found that interleukin-4 (IL-4) abrogated constitutive beta interferon (IFN-beta) synthesis in mouse macrophages. Analysis of endogenous IFN mRNA by reverse transcription-PCR clearly documented the presence of IFN-beta but not of IFN-alpha transcripts in RNA from bone marrow-derived macrophages (BMM). Culture ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.10.6763-6766.1994

    authors: Nickolaus P,Zawatzky R

    更新日期:1994-10-01 00:00:00

  • Properties of noninfectious and transforming viruses released by Murine sarcoma virus-induced hamster tumor cells.

    abstract::The cell culture lines HTG2 and HTG3 were established from a transplantable hamster tumor induced by a murine sarcoma virus (strain Gz-MSV) after 17 and 60 in vivo passages, respectively. The viruses released by these two cell lines markedly differ in morphology, antigenic composition, infectivity, transforming abilit...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.12.4.931-936.1973

    authors: Gazdar AF,Russell E,Sarma PS,Sarin PS,Hall W,Chopra HC

    更新日期:1973-10-01 00:00:00

  • Control of simian immunodeficiency virus SIVmac239 is not predicted by inheritance of Mamu-B*17-containing haplotypes.

    abstract::It is well established that host genetics, especially major histocompatibility complex (MHC) genes, are important determinants of human immunodeficiency virus disease progression. Studies with simian immunodeficiency virus (SIV)-infected Indian rhesus macaques have associated Mamu-B*17 with control of virus replicatio...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01636-06

    authors: Wojcechowskyj JA,Yant LJ,Wiseman RW,O'Connor SL,O'Connor DH

    更新日期:2007-01-01 00:00:00

  • G2/M cell cycle arrest correlates with primate lentiviral Vpr interaction with the SLX4 complex.

    abstract:UNLABELLED:The accessory gene vpr, common to all primate lentiviruses, induces potent G2/M arrest in cycling cells. A recent study showed that human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) mediates this through activation of the SLX4/MUS81/EME1 exonuclease complex that forms part of the Fanconi anem...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02307-14

    authors: Berger G,Lawrence M,Hué S,Neil SJ

    更新日期:2015-01-01 00:00:00

  • Conserved epitopes on the hemagglutinin-neuraminidase proteins of human and bovine parainfluenza type 3 viruses: nucleotide sequence analysis of variants selected with monoclonal antibodies.

    abstract::We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infec...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.60.1.90-96.1986

    authors: Coelingh KJ,Winter CC,Murphy BR,Rice JM,Kimball PC,Olmsted RA,Collins PL

    更新日期:1986-10-01 00:00:00

  • Dengue virus-induced modifications of host cell membranes.

    abstract::Enzymatic markers and electron microscopy were utilized to determine the cellular origin of the membrane types isolated from type 2 dengue virus-infected BHK cells by discontinuous sucrose gradient centrifugation. The results showed an apparent separation of plasma membrane, smooth and rough endoplasmic reticulum with...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.16.4.1017-1026.1975

    authors: Stohlman SA,Wisseman CL Jr,Eylar OR,Silverman DJ

    更新日期:1975-10-01 00:00:00

  • The collagen repeat sequence is a determinant of the degree of herpesvirus saimiri STP transforming activity.

    abstract::Herpesvirus saimiri (HVS) is divided into three subgroups, A, B, and C, based on sequence divergence at the left end of genomic DNA in which the saimiri transforming protein (STP) resides. Subgroup A and C strains transform primary common marmoset lymphocytes to interleukin-2-independent growth, whereas subgroup B str...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.17.8102-8110.2000

    authors: Choi JK,Ishido S,Jung JU

    更新日期:2000-09-01 00:00:00

  • Inter- and intragenus structural variations in caliciviruses and their functional implications.

    abstract::The family Caliciviridae is divided into four genera and consists of single-stranded RNA viruses with hosts ranging from humans to a wide variety of animals. Human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses w...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.78.12.6469-6479.2004

    authors: Chen R,Neill JD,Noel JS,Hutson AM,Glass RI,Estes MK,Prasad BV

    更新日期:2004-06-01 00:00:00

  • Initiation and regulation of simian virus 40 early transcription in vitro.

    abstract::We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.41.2.449-461.1982

    authors: Lebowitz P,Ghosh PK

    更新日期:1982-02-01 00:00:00

  • Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.

    abstract::Human immunodeficiency virus type 1 (HIV-1) Vif recruits a cellular ubiquitin ligase complex to degrade antiviral APOBEC3 enzymes (APOBEC3C-H) and PP2A phosphatase regulators (PPP2R5A to PPP2R5E). While APOBEC3 antagonism is the canonical function of HIV-1 Vif, this viral accessory protein is also known to trigger G2/...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00631-20

    authors: Salamango DJ,McCann JL,Demir Ö,Becker JT,Wang J,Lingappa JR,Temiz NA,Brown WL,Amaro RE,Harris RS

    更新日期:2020-10-14 00:00:00

  • HLA class I-mediated control of HIV-1 in the Japanese population, in which the protective HLA-B*57 and HLA-B*27 alleles are absent.

    abstract::We investigated the effect of HLA class I alleles on clinical parameters for HIV-1 disease progression in the Japanese population, where two strongly protective alleles, HLA-B*57 and HLA-B*27, are virtually nonexistent. HLA-B alleles showed a dominant role, primarily through HLA-B*67:01 and the HLA-B*52:01-C*12:02 hap...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00689-12

    authors: Naruto T,Gatanaga H,Nelson G,Sakai K,Carrington M,Oka S,Takiguchi M

    更新日期:2012-10-01 00:00:00

  • Deoxyribonucleotide pools and deoxyribonucleic acid synthesis in mouse embryo cells infected with three classes of polyoma virus particles.

    abstract::Polyoma virus particles were purified by equilibrium centrifugation in CsCl. Particles from three regions of the density gradient were examined for infectivity, for their ability to induce expanded pools of deoxyribonucleic acid (DNA) precursors, and for their ability to stimulate the synthesis of DNA. The most infect...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.6.1.28-32.1970

    authors: Skoog L,Nordenskjöld BA,Lindberg U

    更新日期:1970-07-01 00:00:00

  • Insertion of capsid proteins from nonenveloped viruses into the retroviral budding pathway.

    abstract::Retroviral Gag proteins direct the assembly and release of virus particles from the plasma membrane. The budding machinery consists of three small domains, the M (membrane-binding), I (interaction), and L (late or "pinching-off") domains. In addition, Gag proteins contain sequences that control particle size. For Rous...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.14.6527-6536.2001

    authors: Krishna NK,Wills JW

    更新日期:2001-07-01 00:00:00

  • The S protein of bovine coronavirus is a hemagglutinin recognizing 9-O-acetylated sialic acid as a receptor determinant.

    abstract::The S protein of bovine coronavirus (BCV) has been isolated from the viral membrane and purified by gradient centrifugation. Purified S protein was identified as a viral hemagglutinin. Inactivation of the cellular receptors by sialate 9-O-acetylesterase and generation of receptors by sialylation of erythrocytes with N...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.11.6232-6237.1991

    authors: Schultze B,Gross HJ,Brossmer R,Herrler G

    更新日期:1991-11-01 00:00:00

  • Nucleotide sequence of the Akv env gene.

    abstract::The sequence of 2,191 nucleotides encoding the env gene of murine retrovirus Akv was determined by using a molecular clone of the Akv provirus. Deduction of the encoded amino acid sequence showed that a single open reading frame encodes a 638-amino acid precursor to gp70 and p15E. In addition, there is a typical leade...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.42.2.519-529.1982

    authors: Lenz J,Crowther R,Straceski A,Haseltine W

    更新日期:1982-05-01 00:00:00

  • The N-terminal region of the luteovirus readthrough domain determines virus binding to Buchnera GroEL and is essential for virus persistence in the aphid.

    abstract::Luteoviruses and the luteovirus-like pea enation mosaic virus (PEMV; genus Enamovirus) are transmitted by aphids in a circulative, nonreplicative manner. Acquired virus particles persist for several weeks in the aphid hemolymph, in which a GroEL homolog, produced by the primary endosymbiont of the aphid, is abundantly...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.10.7258-7265.1997

    authors: van den Heuvel JF,Bruyère A,Hogenhout SA,Ziegler-Graff V,Brault V,Verbeek M,van der Wilk F,Richards K

    更新日期:1997-10-01 00:00:00

  • Strain variation in an emerging iridovirus of warm-water fishes.

    abstract::Although iridoviruses vary widely within and among genera with respect to their host range and virulence, variation within iridovirus species has been less extensively characterized. This study explores the nature and extent of intraspecific variation within an emerging iridovirus of North American warm-water fishes, ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.16.8812-8818.2003

    authors: Goldberg TL,Coleman DA,Grant EC,Inendino KR,Philipp DP

    更新日期:2003-08-01 00:00:00

  • Association of 4S ribonucleic acid with oncornavirus ribonucleic acids.

    abstract::Oncornavirus 60 to 70S ribonucleic acids (RNA), such as those from avian myeloblastosis virus, Schmidt-Ruppin virus, or mouse sarcoma-mouse leukemia viruses, isolated by conventional techniques, contain 4S transferlike RNA molecules that are released upon dissociation of the 60 to 70S RNA with heat. The 4S RNA represe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.8.2.254-256.1971

    authors: Erikson E,Erikson RL

    更新日期:1971-08-01 00:00:00

  • Cloning and characterization of herpes simplex virus type 1 oriL: comparison of replication and protein-DNA complex formation by oriL and oriS.

    abstract::The herpes simplex virus type 1 genome contains three origins of DNA replication: two copies of oriS and one copy of oriL. Although oriS has been characterized extensively, characterization of oriL has been severely limited by the inability to amplify oriL sequences in an undeleted form in Escherichia coli. We report ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.3.1377-1388.1995

    authors: Hardwicke MA,Schaffer PA

    更新日期:1995-03-01 00:00:00

  • Reticuloendotheliosis type C and primate type D oncoretroviruses are members of the same receptor interference group.

    abstract::The reticuloendotheliosis viruses (REVs), originally isolated from avian species, constitute a group of retroviruses which are more closely related to mammalian retroviruses than to other avian retroviruses. The envelope glycoproteins of members of the REV group display a striking amino acid sequence identity with a g...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.6.3448-3454.1992

    authors: Koo HM,Gu J,Varela-Echavarria A,Ron Y,Dougherty JP

    更新日期:1992-06-01 00:00:00

  • Epstein-Barr virus type 2 latently infects T cells, inducing an atypical activation characterized by expression of lymphotactic cytokines.

    abstract:UNLABELLED:Epstein-Barr virus (EBV) is a well-established B-cell-tropic virus associated with various lymphoproliferative diseases of both B-cell and non-B-cell origin. EBV is associated with a number of T-cell lymphomas; however, in vitro studies utilizing prototypical EBV type 1 (EBV-1) laboratory strains have genera...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03001-14

    authors: Coleman CB,Wohlford EM,Smith NA,King CA,Ritchie JA,Baresel PC,Kimura H,Rochford R

    更新日期:2015-02-01 00:00:00

  • HveA (herpesvirus entry mediator A), a coreceptor for herpes simplex virus entry, also participates in virus-induced cell fusion.

    abstract::The purpose of this study was to determine whether a cell surface protein that can serve as coreceptor for herpes simplex virus type 1 (HSV-1) entry, herpesvirus entry mediator (previously designated HVEM but renamed HveA), also mediates HSV-1-induced cell-cell fusion. We found that transfection of DNA from KOS-804, a...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.7.5802-5810.1998

    authors: Terry-Allison T,Montgomery RI,Whitbeck JC,Xu R,Cohen GH,Eisenberg RJ,Spear PG

    更新日期:1998-07-01 00:00:00

  • Specific and common alterations in host gene transcript accumulation following infection of the chestnut blight fungus by mild and severe hypoviruses.

    abstract::We report the use of a cDNA microarray to monitor global transcriptional responses of the chestnut blight fungus, Cryphonectria parasitica, to infection by mild and severe isolates of virulence-attenuating hypoviruses that share 87 to 93% and 90 to 98% identity at the nucleotide and amino acid levels, respectively. In...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.78.8.4145-4155.2004

    authors: Allen TD,Nuss DL

    更新日期:2004-04-01 00:00:00

  • A mutation in the latency-related gene of bovine herpesvirus 1 inhibits protein expression from open reading frame 2 and an adjacent reading frame during productive infection.

    abstract::The latency-related (LR) gene of bovine herpesvirus 1 (BHV-1) is abundantly expressed during latency. A mutant BHV-1 strain that contains three stop codons at the 5' terminus of the LR gene (LR mutant) does not reactivate from latency. This study demonstrates that the LR mutant does not express open reading frame 2 or...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.78.6.3184-3189.2004

    authors: Jiang Y,Inman M,Zhang Y,Posadas NA,Jones C

    更新日期:2004-03-01 00:00:00

  • Widespread endogenization of densoviruses and parvoviruses in animal and human genomes.

    abstract::Parvoviruses infect humans and a broad range of animals, from mammals to crustaceans, and generally are associated with a variety of acute and chronic diseases. However, many others cause persistent infections and are not known to be associated with any disease. Viral persistence is likely related to the ability to in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00828-11

    authors: Liu H,Fu Y,Xie J,Cheng J,Ghabrial SA,Li G,Peng Y,Yi X,Jiang D

    更新日期:2011-10-01 00:00:00