Abstract:
:A panel of monoclonal antibodies that recognize the two major glycoproteins of bovine viral diarrhea virus (BDV) was used to evaluate the antigenic relationship between cytopathic (CP) and noncytopathic (NCP) viruses isolated from cattle dead or dying from fatal BDV infections. Various unrelated BDV isolates were initially screened by indirect immunofluorescence with monoclonal antibodies directed against the 56- to 58- and 48-kilodalton glycoproteins of the virus. A wide spectrum of reactivity that was independent of biotype was found. Biological clones of the same isolate showed only minor variations from the parental isolate, as did isolates taken from different animals located on the same farm. A similar analysis was repeated with pairs of CP and NCP viruses isolated from 16 unrelated clinical cases of BDV infection resulting in fatal disease. The reactivity patterns within individual pairs of isolates taken from the same animals were in most instances very similar and in some cases indistinguishable from one another. The results demonstrate that antigenic similarity between biotypes is a consistent finding in animals dying from fatal BDV infections. In view of the wide degree between biotypes is a consistent finding in animals dying from fatal BDV infections. In view of the wide degree of variation in reactivity patterns between unrelated BDV isolates, the close antigenic similarity of CP BDV to the homologous NCP BDV of a given pair strongly suggests that CP BDV arises by mutation from NCP BDV.
journal_name
J Viroljournal_title
Journal of virologyauthors
Corapi WV,Donis RO,Dubovi EJdoi
10.1128/JVI.62.8.2823-2827.1988subject
Has Abstractpub_date
1988-08-01 00:00:00pages
2823-7issue
8eissn
0022-538Xissn
1098-5514journal_volume
62pub_type
杂志文章abstract::The U(S)3 open reading frame of herpes simplex virus 1 (HSV-1) was reported to encode two mRNAs each directing the synthesis of the same protein. We report that the U(S)3 gene encodes two proteins. The predominant U(S)3 protein is made in wild-type HSV-1-infected cells. The truncated mRNA and a truncated protein desig...
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.65.5.2290-2298.1991
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pub_type: 杂志文章
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doi:10.1128/JVI.44.3.877-881.1982
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1974-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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pub_type: 杂志文章
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