Abstract:
:Four chimeric human immunodeficiency virus type 1 (HIV-1) env genes were constructed which encoded the extracellular domain of either the wild-type or a cleavage-defective HIV-1 envelope glycoprotein (gp160) fused at one of two different positions in env to a C-terminal glycosyl-phosphatidylinositol (GPI) attachment signal from the mouse Thy-1.1 glycoprotein. All four of the constructs encoded glycoproteins that were efficiently expressed when Rev was supplied in trans, and the two cleavable forms were processed normally to gp120 and a chimeric "gp41." The chimeric glycoproteins, in contrast to the wild-type glycoprotein, could be cleaved from the surface of transfected cells by treatment with phosphatidylinositol-specific phospholipase C, indicating that they were anchored in the plasma membrane by a GPI moiety. These GPI-anchored glycoproteins were transported intracellularly at a rate only slightly lower than that of the full-length HIV-1 glycoprotein and were present on the cell surface in equivalent amounts. Nevertheless, all four glycoproteins were defective in mediating both cell-cell and virus-cell fusion as determined by syncytium formation in COS-1-HeLa-T4 cell mixtures and trans complementation of an env-defective HIV-1 genome.
journal_name
J Viroljournal_title
Journal of virologyauthors
Salzwedel K,Johnston PB,Roberts SJ,Dubay JW,Hunter Edoi
10.1128/JVI.67.9.5279-5288.1993subject
Has Abstractpub_date
1993-09-01 00:00:00pages
5279-88issue
9eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::Herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) blocks complement activation, and glycoprotein E (gE) interferes with IgG Fc-mediated activities. While evaluating gC- and gE-mediated immune evasion in human immunodeficiency virus (HIV)-HSV-1-coinfected subjects, we noted that antibody alone was more effective ...
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.749-752.1971
更新日期:1971-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.1984-1991.1997
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更新日期:1969-11-01 00:00:00
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更新日期:1970-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
abstract::V2/V3 conformational epitope antibodies that broadly neutralize HIV-1 (PG9 and PG16) have been recently described. Since an elicitation of previously known broadly neutralizing antibodies has proven elusive, the induction of antibodies with such specificity is an important goal for HIV-1 vaccine development. A critica...
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doi:10.1128/JVI.05045-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.1683-1687.1997
更新日期:1997-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01693-09
更新日期:2010-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 共识发展会议,杂志文章
doi:10.1128/JVI.01251-15
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abstract::The existence of novel endogenous retrovirus elements in the chicken genome, designated EAV-HP, with close sequence identity to the env gene of avian leukosis virus (ALV) subgroup J has been reported (L. M. Smith, A. A. Toye, K. Howes, N. Bumstead, L. N. Payne, and K. Venugopal, J. Gen. Virol. 80:261-268, 1999). To re...
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pub_type: 杂志文章
doi:10.1128/jvi.74.3.1296-1306.2000
更新日期:2000-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.5.401-408.1968
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.299-305.1978
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journal_title:Journal of virology
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更新日期:1977-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1988-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3269-3279.1996
更新日期:1996-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6398-6405.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1983-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
abstract::The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyac...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1979-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00887-19
更新日期:2019-08-13 00:00:00