Abstract:
:Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus related to rhabdoviruses and paramyxoviruses. Unlike animal viruses of these two families, BDV transcribes RNAs in the nuclei of infected cells and produces high levels of transcripts containing multiple open reading frames. Previous Northern blot analysis of RNA from BDV-infected rat brain tissue has shown that two viral transcripts, a 6.1-kb RNA and a 1.5-kb RNA, lack regions that are internal to two otherwise identical transcripts, the 7.1-kb RNA and the 2.8-kb RNA, respectively (T. Briese, A. Schneemann, A. Lewis, Y. Park, S. Kim, H. Ludwig, and W. I. Lipkin, Proc. Natl. Acad. Sci. USA 91:4362-4366, 1994). To determine the precise location of this deletion, we performed reverse transcription PCR analysis using total RNA from BDV-infected rat brain tissue. This investigation resulted in the identification of two introns in the 7.1- and 2.8-kb RNAs, which can be alternatively spliced to yield additional RNA species, including the 6.1- and 1.5-kb RNAs. Transient transfection of COS-7 cells with a cDNA clone of the 2.8-kb RNA resulted in the production of both the 2.8-kb RNA and the 1.5-kb RNA, confirming the theory that the 2.8-kb RNA is a sufficient substrate for splicing in mammalian cells. Splicing has not previously been observed in nonsegmented, negative-strand RNA viruses and presumably serves as a mechanism by which expression of BDV proteins is regulated in infected cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Schneider PA,Schneemann A,Lipkin WIdoi
10.1128/JVI.68.8.5007-5012.1994subject
Has Abstractpub_date
1994-08-01 00:00:00pages
5007-12issue
8eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::To investigate the genetic stability of vaccinia virus DNA, we have tested whether alterations occurred in the polypeptide composition of this complex virus during persistent infections. We found that variants isolated at various passages in Friend erythroleukemia cells persistently infected with vaccinia virus contai...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2642-2647.1987
更新日期:1987-08-01 00:00:00
abstract::A systematic analysis of immune responses on a population level is critical for a human immunodeficiency virus type 1 (HIV-1) vaccine design. Our studies in Botswana on (i) molecular analysis of the HIV-1 subtype C (HIV-1C) epidemic, (ii) frequencies of major histocompatibility complex class I HLA types, and (iii) cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10155-10168.2002
更新日期:2002-10-01 00:00:00
abstract::It has previously been shown that: (i) during infection of its host, the DNA bacteriophage T7 assembles a DNA-free procapsid (capsid I), a capsid with an envelope differing physically and chemically from the capsid of the mature bacteriophage, and (ii) capsid I converts to a capsid (capsid II) with a bacteriophage-lik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.2.583-594.1982
更新日期:1982-05-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) particles typically contain small amounts of the surface envelope protein (SU), and this is widely believed to be due to shedding of SU from mature virions. We purified proteins from HIV-1 and SIV isolates using procedures which allow ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5315-5325.2002
更新日期:2002-06-01 00:00:00
abstract::The temporal regulation of DNA replication is thought to be important for chromosome organization and genome stability. We show here that Epstein-Barr virus (EBV) genomes replicate in mid- to late S phase and that agents that accelerate replication timing of EBV reduce viral genome stability. Hydroxyurea (HU) treatmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02115-08
更新日期:2009-03-01 00:00:00
abstract::Type I interferons induce a complex transcriptional program that leads to a generalized antiviral response against a large panel of viruses, including human immunodeficiency virus type 1 (HIV-1). However, despite the fact that interferons negatively regulate HIV-1 ex vivo, a chronic interferon state is linked to the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02553-12
更新日期:2013-03-01 00:00:00
abstract::Hepatitis A virus (HAV) has previously been reported to bind to human red blood cells through interaction with glycophorin A. Residue K221 of VP1 and the surrounding VP3 residues are involved in such an interaction. This capsid region is specifically recognized by the monoclonal antibody H7C27. A monoclonal antibody-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00707-12
更新日期:2012-08-01 00:00:00
abstract::A retrovirus vector has been developed that selects for instances in which the provirus integrates in close proximity to cellular promoters. Coding sequences for a selectable marker (histidinol dehydrogenase) were inserted into the 3' long terminal repeat (LTR) of an enhancerless Molony murine leukemia virus. Although...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.8.3227-3233.1989
更新日期:1989-08-01 00:00:00
abstract::Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the viral DNA was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.295-302.1977
更新日期:1977-10-01 00:00:00
abstract::The coding capacity of human cytomegalovirus (HCMV) for glycoproteins by far exceeds that of other herpesviruses. Few of these proteins have been characterized so far. We have investigated the gene product of reading frame UL132. The putative protein product of UL132 is a glycoprotein with a theoretical mass of 29.8 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::Vpu is a 16-kDa membrane-associated phosphoprotein that is expressed from the same, singly spliced message as the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein precursor, gp160. Previous studies suggest that Vpu functions in the late stages of viral replication, possibly in virus egression from the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4190-4194.1993
更新日期:1993-07-01 00:00:00
abstract::Coinfection with HIV-1 and Kaposi's sarcoma-associated herpesvirus (KSHV) often leads to AIDS-related malignancies, including Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). The interaction between HIV and KSHV plays a pivotal role in the progression of these malignancies. We have previously demonstrated th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00580-18
更新日期:2018-08-29 00:00:00
abstract::The time course and extent of mengovirus production were the same in metaphase-arrested and interphase L-cells, yet the expression of cytopathology was delayed several hours in metaphase cells. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.2.262-263.1970
更新日期:1970-02-01 00:00:00
abstract::The vaccinia virus H2R gene (VACWR 100) is conserved in all sequenced members of the poxvirus family and encodes a protein with a predicted transmembrane domain and four invariant cysteines. A recombinant vaccinia virus, in which expression of the H2 protein is stringently regulated, was unable to replicate without in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.4744-4754.2005
更新日期:2005-04-01 00:00:00
abstract::The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.131-138.1977
更新日期:1977-01-01 00:00:00
abstract::The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1394-1402.1998
更新日期:1998-02-01 00:00:00
abstract::In order to analyze the function of VPg amplification in aphthoviruses, we have undertaken the first mutational analysis of the repetitive VPg-coding region using an improved foot-and-mouth disease virus (FMDV) cDNA clone from which infective viral RNA was synthesized. A set of VPg mutants was constructed by site-dire...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2251-2260.1992
更新日期:1992-04-01 00:00:00
abstract::Transcriptionally active replication complexes bound to smooth membrane vesicles were isolated from poliovirus-infected cells. In electron microscopic, negatively stained preparations, the replication complex appeared as an irregularly shaped, oblong structure attached to several virus-induced vesicles of a rosettelik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1156-1163.1990
更新日期:1990-03-01 00:00:00
abstract::Simian virus 40 large T antigen (large T) in the early and the late phases of infection differs significantly in its sequence-specific DNA-binding and ATPase activities, indicating that different large-T populations participate in virus-specific events at various stages of the infectious cycle. To further characterize...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2308-2316.1989
更新日期:1989-05-01 00:00:00
abstract::A consensus binding site for the human papillomavirus (HPV) E2 protein was determined from an unbiased set of degenerate oligonucleotides using cyclic amplification and selection of targets (CASTing). Detectable DNA-protein complexes were formed after six to nine cycles of CASTing. A population of selected binding sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1722-1735.2001
更新日期:2001-02-01 00:00:00
abstract::Arenaviruses are rodent-borne viruses with a bisegmented RNA genome. A genetically unique arenavirus, Lujo virus, was recently discovered as the causal agent of a nosocomial outbreak of acute febrile illness with hemorrhagic manifestations in Zambia and South Africa. The outbreak had a case fatality rate of 80%. A rev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01144-12
更新日期:2012-10-01 00:00:00
abstract::The natural life cycle of alphaviruses, a group of plus-strand RNA viruses, involves transmission to vertebrate hosts via mosquitoes. Chronic infections are established in mosquitoes (and usually in mosquito cell cultures), but infection of susceptible vertebrate cells typically results in rapid shutoff of host mRNA t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.3854-3865.1999
更新日期:1999-05-01 00:00:00
abstract::The proteins of purified avian oncoviruses were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and isoelectric focusing. Certain members of the avian leukosis-sarcoma viruses (ALSV) had group-specific antigens with altered electrophoretic properties. (i) The p27 protein of Rous-associated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.3.850-864.1977
更新日期:1977-12-01 00:00:00
abstract::The XC rat cell line was found to support the replication of a strain of the Moloney murine sarcoma-leukemia virus. In growth curve experiments cytopathology was paralleled by the production of murine sarcoma virus and leukemia virus progeny having the biologic, antigenic, and biophysical properties of the infecting v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.1.146-149.1973
更新日期:1973-01-01 00:00:00
abstract::The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4206-4212.1995
更新日期:1995-07-01 00:00:00
abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe acute respiratory infection with as yet unclear epidemiology. We previously showed that MERS-CoV counteracts parts of the innate immune response in human bronchiolar cells. Here we analyzed accessory proteins 3, 4a, 4b, and 5 for their abilities to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01845-13
更新日期:2013-11-01 00:00:00
abstract::Latency-associated nuclear antigen 1 (LANA1) of Kaposi's sarcoma-associated herpesvirus (KSHV) is implicated in the persistence of the viral genome during latent infection. It has been suggested that LANA1 tethers the viral genome to the host chromosome and also participates actively in DNA replication from the termin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7248-7256.2004
更新日期:2004-07-01 00:00:00
abstract::Human CD4(+) T-helper 1 cell responses to Epstein-Barr virus (EBV) infection are likely to be important in the maintenance of virus-specific CD8(+) memory and/or as antiviral effectors in their own right. The present work has used overlapping peptides as stimulators of gamma interferon release (i) to identify CD4(+) e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8649-8659.2001
更新日期:2001-09-01 00:00:00
abstract::We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
abstract::We are using site-directed mutagenesis of single viral genes to identify and analyze the genetic determinants of human and simian immunodeficiency virus pathogenicity. In a first approach, we have constructed a series of simian immunodeficiency virus SIVmac nef mutants by partial deletion and insertions in the nef gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5237-5243.1991
更新日期:1991-10-01 00:00:00