Abstract:
:Nonstructural protein 1 (NS1) of yellow fever virus (YF) is a glycoprotein localized to extracytoplasmic compartments within infected cells. We have previously shown that NS1 can be supplied in trans and is required for viral RNA replication, a process thought to occur in membrane-bound cytoplasmic complexes. Here we report that the NS1 gene from a related virus, dengue virus (DEN), is unable to function in the process of YF RNA replication. This virus-specific incompatibility leads to a lack of initial minus-strand accumulation, suggesting that DEN NS1 is unable to productively interact with the YF replicase. Based on a YF deletion mutant that requires NS1 in trans, a genetic screen for suppressor mutants was used to select virus variants able to utilize DEN NS1. In three independent selections, a single mutation was mapped to the NS4A gene, which encodes a putative transmembrane replicase component. This mutation, as well as several additional mutations, was engineered into the NS1-deficient genome and confirmed a genetic interaction between NS1 and NS4A. These findings suggest a potential mechanism for integrating NS1 into the cytoplasmic process of RNA replication.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lindenbach BD,Rice CMdoi
10.1128/JVI.73.6.4611-4621.1999subject
Has Abstractpub_date
1999-06-01 00:00:00pages
4611-21issue
6eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.3.352-358.1971
更新日期:1971-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.263-272.1972
更新日期:1972-02-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.14.1.133-138.1974
更新日期:1974-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01347-06
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abstract::The hepatitis B virus (HBV) X protein (HBx) is a multifunctional regulator of cellular signal transduction and transcription pathways and has a critical role in HBV replication. Much of the cytoplasmic signal transduction activity associated with HBx expression and its stimulation of viral replication is attributable ...
journal_title:Journal of virology
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doi:10.1128/JVI.80.9.4406-4414.2006
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.2.318-327.1986
更新日期:1986-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01495-20
更新日期:2020-12-09 00:00:00
abstract::The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-05
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abstract::A distinctive feature of chronic human immunodeficiency virus type 1 (HIV-1) infection is the presence of multiple coexisting genetic variants, or subpopulations, that comprise the HIV-1 population detected in the peripheral blood. Analysis of HIV-1 RNA decay dynamics during the initiation of highly active antiretrovi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02486-08
更新日期:2009-05-01 00:00:00
abstract::A fibrosarcoma was induced in a hamster by bovine papilloma virus type 2 (BPV2). The content of BPV2 DNA sequences was measured by DNA-DNA and cRNA-DNA hybridizations. The tumor contained approximately 300 BPV2 genome equivalents per cell. Southern blot hybridization indicated that the viral DNA was in free form, the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.945-949.1981
更新日期:1981-09-01 00:00:00
abstract::Human adenovirus type 12 was propagated on human embryonic kidney cells, and the specific infectivities of intra- and extracellular virus particles were compared between 48 and 104 h after infection. Released virions exhibited a specific infectivity of up to 10 times higher than that of intracellular particles. The in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.1.117-125.1985
更新日期:1985-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) clade C causes >50% of all HIV infections worldwide, and an estimated 90% of all transmissions occur mucosally with R5 strains. A pathogenic R5 simian-human immunodeficiency virus (SHIV) encoding HIV clade C env is highly desirable to evaluate candidate AIDS vaccines in nonh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00558-06
更新日期:2006-09-01 00:00:00
abstract::The human polyomavirus JC virus (JCV) establishes persistent infections in most individuals and is the etiologic agent of progressive multifocal leukoencephalopathy. In this report, we describe the establishment of a soluble cell-free system that is capable of replicating exogenous plasmid DNA containing the JCV origi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7421-7428.1997
更新日期:1997-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.76.1.432-435.2002
更新日期:2002-01-01 00:00:00
abstract::Friend murine leukemia virus (F-MuLV) is a replication-competent, ecotropic, NB-tropic retrovirus which produces a rapidly fatal erythroleukemia in susceptible strains of mice. We previously molecularly cloned the entire F-MuLV genome. Transfection of this cloned DNA into NIH 3T3 mouse fibroblasts produces a virus wit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.718-725.1983
更新日期:1983-06-01 00:00:00
abstract::The immune response to viral glycoproteins is often directed against conformation- and/or glycosylation-dependent structures; synthetic peptides and bacterially expressed proteins are inadequate probes for the mapping of such epitopes. This report describes a retroviral vector system that presents such native epitopes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.400-410.1994
更新日期:1994-01-01 00:00:00
abstract:UNLABELLED:Oncogenesis is frequently accompanied by the activation of specific metabolic pathways. One such pathway is fatty acid biosynthesis, whose induction is observed upon transformation of a wide variety of cell types. Here, we explored how defined oncogenic alleles, specifically the simian virus 40 (SV40) T anti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03671-14
更新日期:2015-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4722-4731.1993
更新日期:1993-08-01 00:00:00
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/jvi.74.5.2393-2405.2000
更新日期:2000-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.124-130.1986
更新日期:1986-10-01 00:00:00
abstract::Neurotropic coronavirus induces acute encephalomyelitis and demyelination in mice. Infection of BALB/c (H-2(d)) mice expressing a dominant negative gamma interferon (IFN-gamma) receptor specifically in oligodendrocytes was examined to determine the influence of IFN-gamma signaling on pathogenesis. Inhibition of IFN-ga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02373-09
更新日期:2010-03-01 00:00:00
abstract::Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.299-305.1978
更新日期:1978-05-01 00:00:00
abstract::Adult T-cell leukemia (ATL) is a fatal malignancy of CD4+ T cells infected with human T-cell leukemia virus type 1 (HTLV-1). ATL cells often exhibit random gross chromosomal rearrangements that are associated with the induction and improper repair of double-stranded DNA breaks (DSBs). The viral oncoprotein Tax has bee...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00672-18
更新日期:2018-07-17 00:00:00
abstract::Autographa californica nuclear polyhedrosis virus late and very late mRNAs are transcribed by an RNA polymerase consisting of four virus-encoded polypeptides: LEF-8, LEF-9, LEF-4, and p47. The 464-amino-acid LEF-4 subunit contains the signature motifs of GTP:RNA guanylyltransferases (capping enzymes). Here, we show th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10020-10028.1998
更新日期:1998-12-01 00:00:00
abstract::The human herpes simplex virus (HSV) protein VP16 induces formation of a transcriptional regulatory complex with two cellular factors-the POU homeodomain transcription factor Oct-1 and the cell proliferation factor HCF-1-to activate viral immediate-early-gene transcription. Although the cellular role of Oct-1 in trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.12402-12411.2001
更新日期:2001-12-01 00:00:00
abstract::Despite the long-standing observation that herpes simplex virus (HSV) latency-associated transcript (LAT) promoter deletion viruses show impaired recurrence phenotypes in relevant animal models, the mechanism by which these sequences exert this phenotypic effect is unknown. We constructed and evaluated four mutant HSV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00642-18
更新日期:2018-06-29 00:00:00
abstract:UNLABELLED:Although the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) epidemic was controlled by nonvaccine measures, coronaviruses remain a major threat to human health. The design of optimal coronavirus vaccines therefore remains a priority. Such vaccines present major challenges: coronavirus im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02980-14
更新日期:2015-03-01 00:00:00
abstract::The hepatitis B virus (HBV) X protein (pX) is implicated in hepatocarcinogenesis by an unknown mechanism. pX variants encoded by HBV genomes found integrated in genomic DNA from liver tumors of patients with hepatocellular carcinoma (HCC) generally lack amino acids 134 to 154. Since deregulation of mitogenic pathways ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00661-06
更新日期:2006-11-01 00:00:00