Abstract:
:Oligomer formation of the gB glycoprotein of herpes simplex virus type 1 was studied by sedimentation analysis of radioactively labeled infected cell and virion lysates. Fractions from sucrose gradients were precipitated with a pool of gB-specific monoclonal antibodies and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Pulse-labeled gB from infected cell was synthesized as monomers and converted to oligomers posttranslationally. The oligomers from infected cells and from virions sedimented as dimers, and there was no evidence of higher-molecular-weight forms. To identify amino acid sequences of gB that contribute to oligomer formation, pairs of mutant plasmids were transfected into Vero cells and superinfected with a gB-null mutant virus to stimulate plasmid-specified gene expression. Radioactively labeled lysates were precipitated with antibodies and examined by SDS-PAGE. Polypeptides from cotransfections were precipitated with an antibody that recognized amino acid sequences present in only one of the two polypeptides. A coprecipitated polypeptide lacking the antibody target epitope was presumed to contain the sequences necessary for oligomer formation. Using this technique, two noncontiguous sites for oligomer formation were detected. An upstream site was localized between residues 93 and 282, and a downstream site was localized between residues 596 and 711. Oligomer formation resulted from molecular interactions between two upstream sites, between two downstream sites, and between an upstream and a downstream site. A schematic diagram of a gB oligomer is presented that is consistent with these data.
journal_name
J Viroljournal_title
Journal of virologyauthors
Highlander SL,Goins WF,Person S,Holland TC,Levine M,Glorioso JCdoi
10.1128/JVI.65.8.4275-4283.1991subject
Has Abstractpub_date
1991-08-01 00:00:00pages
4275-83issue
8eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3330-3338.1992
更新日期:1992-06-01 00:00:00
abstract::The fate of polyomavirus genomes in stable transformants was examined in experiments in which rat or hamster cells were infected with mixed viral populations containing either two distinguishable wild types or a wild type plus a transformation-deficient mutant. The results demonstrate that in 90% of the cases in eithe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1304-1313.1990
更新日期:1990-03-01 00:00:00
abstract::Intracisternal-A-particle-related envelope-encoding (IAPE) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of IAPEs as infectious agents. To test IAPE mobility and potential transmission in mice, we have analyzed the distribution of IAPE elements in the gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6450-6454.1996
更新日期:1996-09-01 00:00:00
abstract::In two cases of parenteral transmission of human immunodeficiency virus type 1 (HIV-1) syncitium-inducing (SI) variants, we previously observed selection for macrophagetropic variants. Although infection of macrophages is generally mediated via CCR5, we found no selection for SI variants that could use CCR5 as corecep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8848-8853.2001
更新日期:2001-09-01 00:00:00
abstract::The tempo and intensity of retroviral neuropathogenesis are dependent on the capacity of the virus to invade the central nervous system. For murine leukemia viruses, an important determinant of neuroinvasiveness is the virus-encoded protein glycosylated Gag, the function of which in the virus life cycle is not known. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5619-5625.1998
更新日期:1998-07-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::The severe acute respiratory syndrome coronavirus (SARS-CoV) was recently identified as the etiology of SARS. The virus particle consists of four structural proteins: spike (S), small envelope (E), membrane (M), and nucleocapsid (N). Recognition of a specific sequence, termed the packaging signal (PS), by a virus N pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.13848-13855.2005
更新日期:2005-11-01 00:00:00
abstract::Treatment of cells with lectins specific for N-acetylglucosamine (GlcNAc) blocked infection by mouse cytomegalovirus (MCMV), and GlcNAc pretreatment of the lectin blocked this effect. MCMV failed to infect N-acetylglucosaminidase (GlcNAcase)-treated mouse embryo fibroblasts (MEF). GlcNAc and GlcNAc-containing syntheti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5430-5440.1990
更新日期:1990-11-01 00:00:00
abstract::Recombinant vaccinia viruses expressing C-terminally truncated E's that ranged in length from 9 to 99% of the N-terminal sequence were constructed. The overall antigenicity of the E products was analyzed by radioimmunoprecipitation, using dengue virus hyperimmune mouse ascitic fluid (HMAF) or an anti-E peptide serum. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1400-1407.1991
更新日期:1991-03-01 00:00:00
abstract::Extracts made from human respiratory syncytial virus (RSV)-infected Hep-2 cells synthesized mRNAs encoded by all known viral genes. In contrast, RSV ribonucleoproteins purified from infected cells failed to transcribe in vitro; transcription was restored by addition of a cytoplasmic extract of uninfected Hep-2 cells, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6813-6818.1992
更新日期:1992-11-01 00:00:00
abstract::Viruses can induce progressive neurologic disorders associated with diverse pathological manifestations, and therefore, viral infection of the brain can impair differentiated neural functions, depending on the initial viral tropism. We have previously reported that canine distemper virus (CDV) targets certain mouse br...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7317-7327.1999
更新日期:1999-09-01 00:00:00
abstract::The contributions of the glycoproteins gI (ORF67) and gE (ORF68) to varicella-zoster virus (VZV) replication were investigated in deletion mutants made by using cosmids with VZV DNA derived from the Oka strain. Deletion of both gI and gE prevented virus replication. Complete deletion of gI or deletions of 60% of the N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8279-8288.1997
更新日期:1997-11-01 00:00:00
abstract::The duck hepatitis B virus (DHBV) pregenomic RNA is a bicistronic mRNA encoding the core and polymerase proteins. Thirteen AUGs (C2 to C14) and 10 stop codons (S1 to S10) are located between the C1 AUG for the core protein and the P1 AUG that initiates polymerase translation. We previously found that the translation o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01263-08
更新日期:2009-02-01 00:00:00
abstract::The sequence relations between Kirsten murine sarcoma virus (Ki-SV), Harvey murine sarcoma virus (Ha-SV), and a rat endogenous 30S RNA were studied by electron microscope heteroduplex analysis. The sequence relationships between the sarcoma viruses and their respective parental murine leukemia viruses (Kirsten and Mol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.752-760.1979
更新日期:1979-09-01 00:00:00
abstract::Necroptosis, a regulated form of necrotic cell death, requires the activation of the RIP3 kinase. Here, we identify that infection of host cells with reovirus can result in necroptosis. We find that necroptosis requires sensing of the genomic RNA within incoming virus particles via cytoplasmic RNA sensors to produce t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02404-16
更新日期:2017-02-28 00:00:00
abstract::Infection of human dermal microvascular endothelial (HMVEC-d) cells and human foreskin fibroblast (HFF) cells in vitro by Kaposi's sarcoma-associated herpesvirus (KSHV) provides an excellent in vitro model system to study viral latency. KSHV infection is characterized by the induction of preexisting host signal cascad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00231-06
更新日期:2006-07-01 00:00:00
abstract::Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02259-16
更新日期:2017-05-12 00:00:00
abstract::It is generally accepted that the fitness cost of resistance mutations plays a role in the persistence of transmitted drug-resistant human immunodeficiency virus type 1 and that mutations that confer a high fitness cost are less able to persist in the absence of drug pressure. Here, we show that the fitness cost of re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02712-06
更新日期:2007-03-01 00:00:00
abstract::Polyomavirus large T antigen has an N-terminal domain of approximately 260 amino acids which can immortalize primary cells but lacks sequences known to be required for DNA binding and replication. Treatment of full-length large T with either V8 protease or chymotrypsin yields an N-terminal fragment of 36 to 40 kDa and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.668-673.1994
更新日期:1994-02-01 00:00:00
abstract:UNLABELLED:Venezuelan and western equine encephalitis viruses (VEEV and WEEV; Alphavirus; Togaviridae) are mosquito-borne pathogens causing central nervous system (CNS) disease in humans and equids. Adult CD-1 mice also develop CNS disease after infection with VEEV and WEEV. Adult CD-1 mice infected by the intranasal (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03219-15
更新日期:2016-05-27 00:00:00
abstract::The efficiency and specificity of gene transfer with human adenovirus (hAd)-derived gene transfer vectors would be improved if the native viral tropism could be modified. Here, we demonstrate that the minor capsid protein IX (pIX), which is present in 240 copies in the Ad capsid, can be exploited as an anchor for hete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.7.3470-3479.2004
更新日期:2004-04-01 00:00:00
abstract::Three fish retroviruses infecting walleyes constitute the recently recognized genus called epsilonretrovirus. The founding member of this group, walleye dermal sarcoma virus (WDSV), induces benign skin tumors in the infected fish and replicates near 4 degrees C. While the viral genomic sequence is known, biochemical c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4341-4349.2002
更新日期:2002-05-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::When human adenovirus type 2 or 12 infects cells, either productively or non-productively, three classes of viral deoxyribonucleic acid (DNA) are found within the cells: (i) viral DNA which cosediments with DNA extracted from infectious adenovirions at 31.3S for adenovirus type 2 and at 29.0S for adenovirus type 12, (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.707-719.1971
更新日期:1971-06-01 00:00:00
abstract::Human papillomaviruses (HPV) link their life cycles to epithelial differentiation and induce productive replication of viral DNA in suprabasal cells. Viral-DNA amplification requires cells to remain active in the cell cycle upon differentiation. This is in contrast to normal cells, which lose proliferative capability ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00078-10
更新日期:2010-05-01 00:00:00
abstract::A large percentage of the repetitive elements in mammalian genomes are retroelements, which have been moved primarily by LINE-1 retrotransposons and endogenous retroviruses. Although LINE-1 elements have remained active throughout the mammalian radiation, specific groups of endogenous retroviruses generally remain act...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14698-14707.2005
更新日期:2005-12-01 00:00:00
abstract::We have synthesized 32P-labeled hybridization probes from a hyperdivergent region (nucleotides 51 to 392) of the rotavirus gene encoding the VP7 glycoprotein by using the polymerase chain reaction method. Both RNA (after an initial reverse transcription step) and cloned cDNA from human rotavirus serotypes 1 through 4 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.4021-4024.1990
更新日期:1990-08-01 00:00:00
abstract::A cDNA fragment coding for poliovirus capsid polypeptide VP1 was inserted into a simian virus 40 (SV40) genome in the place of the SV40 VP1 gene and fused in phase to the 3' end of the VP2-VP3 genes. Simian cells were infected with the resulting hybrid virus in the presence of an early SV40 mutant used as a helper. In...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3862-3869.1987
更新日期:1987-12-01 00:00:00
abstract::The matrix (M1) protein of influenza virus is a major structural component, involved in regulation of viral ribonucleoprotein transport into and out of the nucleus. Early in infection, M1 is distributed in the nucleus, whereas later, it is localized predominantly in the cytoplasm. Using immunofluorescence microscopy a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.439-445.1995
更新日期:1995-01-01 00:00:00
abstract::Two strains of minute virus of mice (MVM) show different host cell specificities. The prototype strain MVM(p) grows in fibroblasts, whereas the immunosuppressive variant MVM(i) grows in T lymphocytes. In this study, we have mapped on the viral genome a cell type-specific determinant: it is located between 69 and 85 ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.552-557.1988
更新日期:1988-02-01 00:00:00