Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli.

abstract：:Primary human embryonic kidney (HEK) cells were transformed by a focus assay with BK virus (BKV) DNA molecularly cloned at its unique EcoRI site. Both viral DNA sequences and viral tumor antigens were present and expressed in all the foci that we examined. However, cells isolated from foci were incapable of growth in ...

journal_title：Journal of virology

authors： Pater A,Pater MM

更新日期：1986-05-01 00:00:00

abstract：:Several C/EBP binding sites within the Rous sarcoma virus (RSV) long terminal repeat (LTR) and gag enhancers were mutated, and the effect of these mutations on viral gene expression was assessed. Minimal site-specific mutations in each of three adjacent C/EBP binding sites in the LTR reduced steady-state viral RNA lev...

journal_title：Journal of virology

authors： Ryden TA,de Mars M,Beemon K

更新日期：1993-05-01 00:00:00

abstract：:Sequence analysis of the short unique (Us) segment of the bovine herpesvirus 1 (BHV-1) genome predicted that the Us open reading frame (ORF) 4 encodes a protein with homology to glycoprotein G (gG) of other alpha-herpesviruses (P. Leung-Tack, J.-C. Audonnet, and M. Riviere, Virology 199:409-421, 1994). RNA analysis sh...

journal_title：Journal of virology

authors： Keil GM,Engelhardt T,Karger A,Enz M

更新日期：1996-05-01 00:00:00

abstract：:Analysis of purified simian rotavirus has shown that it contains fewer structural polypeptide classes than previously reported. Two polypeptides (molecular weights, 62,000 and 28,000) commonly found in purified rotaviruses were, in fact, produced by cleavage of a larger structural polypeptide (molecular weight, about ...

journal_title：Journal of virology

authors： Espejo RT,López S,Arias C

更新日期：1981-01-01 00:00:00

abstract：:In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant...

journal_title：Journal of virology

authors： Tekes G,Hofmann-Lehmann R,Stallkamp I,Thiel V,Thiel HJ

更新日期：2008-02-01 00:00:00

abstract：:During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....

journal_title：Journal of virology

authors： Rice SA,Long MC,Lam V,Spencer CA

更新日期：1994-02-01 00:00:00

abstract：:We investigated the cis-acting sequences involved in termination of vesicular stomatis virus mRNA synthesis by using bicistronic genomic analogs. All of the cis-acting signals necessary for termination reside within the first 13 nucleotides of the 23-nucleotide conserved gene junction. This 13-nucleotide termination s...

journal_title：Journal of virology

authors： Barr JN,Whelan SP,Wertz GW

更新日期：1997-11-01 00:00:00

abstract：:Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a w...

journal_title：Journal of virology

authors： Kumar S,Nayak B,Collins PL,Samal SK

更新日期：2011-07-01 00:00:00

abstract：:Thirteen point mutations targeting predicted domains conserved in homologous protein kinases were introduced into the UL97 coding region of the human cytomegalovirus. All mutagenized proteins were expressed in cells infected with recombinant vaccinia viruses (rVV). Several mutations drastically reduced ganciclovir (GC...

journal_title：Journal of virology

authors： Michel D,Kramer S,Höhn S,Schaarschmidt P,Wunderlich K,Mertens T

更新日期：1999-10-01 00:00:00

abstract：:The reasons for poor CD4+ T-cell recovery in some human immunodeficiency virus (HIV)-infected subjects despite effective highly active antiretroviral therapy (HAART) remain unclear. We recently reported that CXCR4-using (X4) HIV-1 could be gradually selected in cellular reservoirs during sustained HAART. Because of th...

journal_title：Journal of virology

authors： Delobel P,Nugeyre MT,Cazabat M,Sandres-Sauné K,Pasquier C,Cuzin L,Marchou B,Massip P,Cheynier R,Barré-Sinoussi F,Izopet J,Israël N

更新日期：2006-10-01 00:00:00

abstract：:We have developed a complementation assay, using transiently transfected COS cells, to facilitate a molecular analysis of the herpes simplex virus type 1 glycoprotein gH. When infected by a gH-null syncytial virus, COS cells expressing wild-type gH generate infectious progeny virions and form a syncytium with neighbor...

journal_title：Journal of virology

authors： Wilson DW,Davis-Poynter N,Minson AC

更新日期：1994-11-01 00:00:00

abstract：:Infectious pancreatic necrosis virus of fish, infectious bursal disease virus of chickens, Tellina virus and oyster virus of bivalve molluscs, and drosophila X virus of Drosophila melanogaster are naked icosahedral viruses with an electron microscopic diameter of 58 to 60 nm. The genome of each of these viruses consis...

journal_title：Journal of virology

authors： Dobos P,Hill BJ,Hallett R,Kells DT,Becht H,Teninges D

更新日期：1979-11-01 00:00:00

abstract：:Cells latently infected with herpes simplex virus (HSV) contain nucleosomal DNA similar to that of host cell chromatin. Recent studies have demonstrated that histones in the latency-associated transcript (LAT) promoter and intron regions contain histone modifications permissive for transcription. However, those histon...

journal_title：Journal of virology

authors： Chen Q,Lin L,Smith S,Huang J,Berger SL,Zhou J

更新日期：2007-05-01 00:00:00

abstract：:Disruption of Epstein-Barr virus (EBV) latency is mediated by ZEBRA, the protein product of the immediate-early EBV gene, BZLF1. In vitro, phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), induces reactivation of EBV. However, the physiological stimuli responsible for the disruption ...

journal_title：Journal of virology

authors： Fahmi H,Cochet C,Hmama Z,Opolon P,Joab I

更新日期：2000-07-01 00:00:00

abstract：:A cell model of primary macrophages isolated from the peritoneal cavity of flavivirus-susceptible and congenic resistant mice has been used to study the extent and kinetics of antiviral effects against West Nile virus upon priming with alpha/beta interferon (IFN-alpha/beta) or poly(I-C) (pIC). The pattern of flaviviru...

journal_title：Journal of virology

authors： Pantelic L,Sivakumaran H,Urosevic N

更新日期：2005-02-01 00:00:00

abstract：UNLABELLED:Replication-deficient rabies virus (RABV)-based vaccines induce rapid and potent antibody responses via T cell-independent and T cell-dependent mechanisms. To further investigate early events in vaccine-induced antibody responses against RABV infections, we studied the role of macrophages as mediators of RAB...

journal_title：Journal of virology

authors： Lytle AG,Shen S,McGettigan JP

更新日期：2015-03-01 00:00:00

abstract：:Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...

journal_title：Journal of virology

authors： Meyer PR,Matsuura SE,Zonarich D,Chopra RR,Pendarvis E,Bazmi HZ,Mellors JW,Scott WA

更新日期：2003-06-01 00:00:00

abstract：:In cell-free systems, 2-5A [ppp(A2'p)nA, n = 2 to greater than or equal to 4] activates a latent endoribonuclease, the 2-5A-dependent RNase, which cleaves rRNA in intact ribosomes into discrete and characteristic products (D. H. Wreschner et al., Nucleic Acids Res. 9:1571-1581, 1981). Here we present Northern blots wh...

journal_title：Journal of virology

authors： Silverman RH,Skehel JJ,James TC,Wreschner DH,Kerr IM

更新日期：1983-06-01 00:00:00

abstract：:Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (Delta NS1/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary hum...

journal_title：Journal of virology

authors： Spann KM,Tran KC,Chi B,Rabin RL,Collins PL

更新日期：2004-04-01 00:00:00

abstract：:The four major Epstein-Barr virion envelope components were separated by column chromatography and reconstituted into artificial liposomes. These liposomes were tested for their ability to bind selectively to Epstein-Barr virus receptor-positive cells. Only when the two high-molecular-weight glycoproteins, VE1 and VE2...

journal_title：Journal of virology

authors： Wells A,Koide N,Klein G

更新日期：1982-01-01 00:00:00

abstract：:Hamster cells are completely nonpermissive for the replication of human adenovirus type 12 (Ad12), whereas types 2 and 5 can replicate in hamster cells. The Ad5-transformed hamster cell line BHK297-C131, which carries the left terminal 18.7% of the Ad5 genome and expresses at least the viral E1A region, can somehow co...

journal_title：Journal of virology

authors： Schiedner G,Schmitz B,Doerfler W

更新日期：1994-09-01 00:00:00

abstract：:The gene coding for the S protein, the smaller of the two envelope antigens of the duck hepatitis B virus (DHBV), is transcribed from a TATA-less promoter. In this study, we localized the promoter to a 245-bp segment of the genome that was capable of efficiently driving expression of a linked reporter gene upon transi...

journal_title：Journal of virology

authors： Welsheimer T,Newbold JE

更新日期：1996-12-01 00:00:00

abstract：:We have previously shown that expression of the adenovirus E1A 12S or 13S products in NIH 3T3 fibroblasts induces susceptibility to the cytotoxic actions of tumor necrosis factor alpha (TNF alpha). A large number of studies have mapped the multiple biological functions of the 12S and 13S products to three highly conse...

journal_title：Journal of virology

authors： Ames RS,Holskin B,Mitcho M,Shalloway D,Chen MJ

更新日期：1990-09-01 00:00:00

abstract：:In order to study cell tropism and attenuation of hepatitis A virus (HAV), the genome of HAV wild-type GBM and two cell culture-adapted variants, GBM/FRhK and GBM/HFS, were cloned and sequenced after amplification by reverse transcriptase-PCR. During virus cultivation, the HAV variant GBM/FRhK had a strict host range ...

journal_title：Journal of virology

authors： Graff J,Normann A,Feinstone SM,Flehmig B

更新日期：1994-01-01 00:00:00

abstract：UNLABELLED:Enterovirus 71 (EV71) recruits various cellular factors to assist in the replication and translation of its genome. Identification of the host factors involved in the EV71 life cycle not only will enable a better understanding of the infection mechanism but also has the potential to be of use in the developm...

journal_title：Journal of virology

authors： Zhang H,Song L,Cong H,Tien P

更新日期：2015-10-01 00:00:00

abstract：:Several transgenic mouse models have been developed which facilitate the transmission of chronic wasting disease (CWD) of cervids and allow prion strain discrimination. The present study was designed to assess the susceptibility of the prototypic mouse line, Tg(CerPrP)1536(+/-), to bovine spongiform encephalopathy (BS...

journal_title：Journal of virology

authors： Vickery CM,Lockey R,Holder TM,Thorne L,Beck KE,Wilson C,Denyer M,Sheehan J,Marsh S,Webb PR,Dexter I,Norman A,Popescu E,Schneider A,Holden P,Griffiths PC,Plater JM,Dagleish MP,Martin S,Telling GC,Simmons MM,Spiro

更新日期：2014-02-01 00:00:00

abstract：:Recombinant DNA clones of a viral clone of spleen necrosis virus, an avian retrovirus, were found to have long terminal repeats of different sizes. The variation was in the U3 region of the long terminal repeats, and any one clone had U3 of the same size in both long terminal repeats. The U3 regions in the 5' and 3' l...

journal_title：Journal of virology

authors： Shimotohno K,Temin HM

更新日期：1982-01-01 00:00:00

abstract：:Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have examined the prM/M and a portion of the E protein for a panel of 38 wild strains of YF virus from Africa representing ...

journal_title：Journal of virology

authors： Mutebi JP,Wang H,Li L,Bryant JE,Barrett AD

更新日期：2001-08-01 00:00:00

abstract：:This article has been retracted. ...

journal_title：Journal of virology

authors： He CQ,Ding NZ,Han GZ,Liu W

更新日期：2008-01-23 00:00:00

abstract：:Grasshoppers, Melanoplus sanguinipes (F.), infected with the grasshopper inclusion body virus (GIBV) showed a general torpor, took longer to develop, and had abnormally high rates of mortality. Infection was found only in the fat body, and developing viruses and inclusion bodies were observed in the nuclei and cytopla...

journal_title：Journal of virology

authors： Henry JE,Nelson BP,Jutila JW

更新日期：1969-06-01 00:00:00