Abstract:
:The investigation of papillomavirus chromatin has been hampered by the unavailability of a tissue culture system for vegetative growth of these viruses. We have used, therefore, bovine papillomavirus type 1-transformed hamster embryo fibroblasts containing 200 to 250 episomal genome equivalents per cell as a source of viral chromatin. The selectively isolated chromatin was shown to be slightly larger (80S) than the mature simian virus 40 chromatin, which was cosedimented in a sucrose density gradient. Both Fo I and Fo II were present in the bovine papillomavirus type 1 chromatin. A fast-sedimenting fraction, whose structure is still unknown, also contained oligomeric bovine papillomavirus type 1 DNA. By in situ DNase digestion of isolated nuclei and subsequent cleavage of the bovine papillomavirus type 1 DNA with various restriction endonucleases, a major DNase-hypersensitive region was detected in the chromatin. This region, comprising approximately 320 base pairs, is located between the relative physical map positions 0.88 and 0.92.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rösl F,Waldeck W,Sauer Gdoi
10.1128/JVI.46.2.567-574.1983subject
Has Abstractpub_date
1983-05-01 00:00:00pages
567-74issue
2eissn
0022-538Xissn
1098-5514journal_volume
46pub_type
杂志文章abstract::Human immunodeficiency virus type 1 (HIV-1) specifically incorporates the host cell peptidyl-prolyl isomerase cyclophilin A into virions via contacts with the capsid (CA) domain of the Gag polyprotein Pr55gag. The immunosuppressant drug cyclosporin A and the nonimmunosuppressive cyclosporin A analog SDZ NIM 811 bind t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5751-5757.1996
更新日期:1996-09-01 00:00:00
abstract::We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3168-3177.1997
更新日期:1997-04-01 00:00:00
abstract::An adeno-associated virus (AAV) genome with a Lys-to-His (K340H) mutation in the consensus nucleotide triphosphate binding site of the rep gene has a dominant-negative DNA replication phenotype in vivo. We expressed both wild-type (Rep78) and mutant (Rep78NTP) proteins in two helper-free expression systems consisting ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1236-1240.1992
更新日期:1992-02-01 00:00:00
abstract::In vitro translation of the three single-stranded RNAs transcribed in vitro by bacteriophage phi 6 RNA polymerase revealed that the large RNA codes for phage proteins P1, P2, P4, and P7, the medium RNA codes for P3, P6, and P10, and the smaller RNA for P5, P8, and P9. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.1.249-251.1980
更新日期:1980-07-01 00:00:00
abstract::An unusual set of 3' coterminal, spliced mRNAs transcribed through the BamHI A fragment have been previously identified in nasopharyngeal carcinoma (NPC) tissues. These RNAs have also been detected at low levels in Burkitt's lymphoma (BL) cell lines and Epstein-Barr virus (EBV)-transformed lymphocytes. Sequence analys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.2765-2771.1997
更新日期:1997-04-01 00:00:00
abstract::Two types of empty capsid particles that differed with respect to the presence of the two outer shell proteins were isolated from MA-104 cells infected with bovine rotavirus V1005. Three previously uncharacterized polypeptides, I, II, and III, migrating between VP2 and VP6, were detected in empty capsids but not in si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3635-3642.1990
更新日期:1990-08-01 00:00:00
abstract::Bacterial lipopolysaccharide (LPS) induces interferon (IFN) secretion and an antiviral state in murine peritoneal macrophages (PM). These cells secrete predominantly IFN-beta, as shown by neutralization assays with monoclonal antibodies. Secretion of IFN-beta is also induced in PM by IFN-gamma. LPS and IFN-gamma syner...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2785-2789.1989
更新日期:1989-06-01 00:00:00
abstract::A tumorigenic line of mouse cells transformed by the nononcogenic murine K papovavirus was established and characterized. Tumor-bearing animals produced antibody specific for K virus-transformed cells; the K virus T antiserum did not react with polyomavirus-transformed cells. Immunoprecipitation of the T antigen in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.954-956.1984
更新日期:1984-06-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a human herpesvirus capable of establishing a latent state in B lymphocytes. EBV's BZLF1 gene product plays a central role in regulating the switch from latency to productive infection. Here, we identify a sequence element, 5'-CAGGTA-3', called ZV, located at nucleotides -17 to -12 relative...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.867-877.2001
更新日期:2001-01-01 00:00:00
abstract::Enveloped virus vectors are used in a wide variety of applications. We have discovered that treatment of cultured cells with phosphatidylserine (PS) liposomes can increase virus vector infection by up to 20-fold. This effect does not abrogate virus receptor requirements, is specific to PS compared to other phospholipi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11496-11500.2005
更新日期:2005-09-01 00:00:00
abstract::The genomic RNA 3' noncoding region is believed to be a major cis-acting molecular genetic determinant for regulating picornavirus negative-strand RNA synthesis by promoting replication complex recognition. We report the replication of two picornavirus RNAs harboring complete deletions of the genomic RNA 3' noncoding ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8868-8874.1997
更新日期:1997-11-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae, is a pathogen of cloven-hoofed animals and causes a disease of major economic importance. Picornavirus-infected cells show changes in cell morphology and rearrangement of cytoplasmic membranes, which are a consequence of virus replication. We show he...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00907-08
更新日期:2008-11-01 00:00:00
abstract::Cytomegalovirus (CMV) retinitis is an important ocular complication in human immunodeficiency virus-infected individuals and the leading cause of blindness in those not undergoing highly active antiretroviral therapy. Murine CMV (MCMV) infection of mice has been shown to be a useful small-animal model for the study of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9165-9175.2002
更新日期:2002-09-01 00:00:00
abstract::Herpes simplex virus (HSV) has often been suggested for development as a vector, particularly for the nervous system. Considerable evidence has shown that for use of HSV as a vector, immediate-early (IE) gene expression must be minimized or abolished, otherwise such vectors are likely to be highly cytotoxic. Mutations...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7399-7409.1999
更新日期:1999-09-01 00:00:00
abstract::Respiratory syncytial virus (RSV) G protein deletion mutants replicate effectively in vitro but have not been detected in nature. Subtyping of RSV strains in hospitalized children in South Africa identified G protein PCR amplicons significantly reduced in size in 2 out of 209 clinical specimens screened over 4 years. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00674-11
更新日期:2011-08-01 00:00:00
abstract::This paper is concerned with the physiological role(s) of T4 phage-coded dihydrofolate reductase, which functions both in DNA precursor metabolism and as a virion protein. (i) We have detected enzyme activity in noninfectious particles produced under restrictive conditions by gene 11 mutants. This supports the conclus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.645-658.1977
更新日期:1977-09-01 00:00:00
abstract::Evidence is presented by use of radiolabeling and pancreatic and T1 ribonuclease digestion that some of the ribonucleic acid specified by herpes simplex virus contains polyadenylic acid sequences. The polyadenylic sequences are not transcribed from viral DNA. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.4.875-879.1972
更新日期:1972-10-01 00:00:00
abstract::Betapapillomavirus replication and transcription have not been studied in detail because of a lack of suitable cellular systems supporting human papillomavirus (HPV) genome replication. We have recently shown that the human osteosarcoma cell line U2OS provides a useful environment for the genome replication of many di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01841-13
更新日期:2014-01-01 00:00:00
abstract::The human cytomegalovirus (HCMV) major immediate-early (MIE) enhancer contains five functional cyclic AMP (cAMP) response elements (CRE). Because the CRE in their native context do not contribute appreciably to MIE enhancer/promoter activity in lytically infected human fibroblasts and NTera2 (NT2)-derived neurons, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01524-06
更新日期:2007-06-01 00:00:00
abstract::Autophagy and the effects of its inhibition or induction were investigated during the entire infectious cycle of varicella-zoster virus (VZV), a human herpesvirus. As a baseline, we first enumerated the number of autophagosomes per cell after VZV infection compared with the number after induction of autophagy followin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02646-13
更新日期:2014-01-01 00:00:00
abstract::The trans-activator Tat proteins coded by human immunodeficiency virus type 1 (HIV-1) and HIV-2 appear to be similar in structure and function. However, the Tat protein of HIV-2 (Tat2) activates the HIV-1 long terminal repeat (LTR) less efficiently than Tat1 (M. Emerman, M. Guyader, L. Montagnier, D. Baltimore, and M....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2031-2036.1992
更新日期:1992-04-01 00:00:00
abstract:UNLABELLED:The majority of currently circulating influenza A(H1N1) viruses are antigenically similar to the virus that caused the 2009 influenza pandemic. However, antigenic variants are expected to emerge as population immunity increases. Amino acid substitutions in the hemagglutinin protein can result in escape from ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02962-14
更新日期:2015-04-01 00:00:00
abstract::Engineered alphavirus vectors expressing reporters of infection have been used for a number of years due to their relatively low costs for analysis of virus replication and the capacity to utilize imaging systems for longitudinal measurements of growth within single animals. In general, these vectors have been derived...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02990-13
更新日期:2014-02-01 00:00:00
abstract::The current therapy for hepatitis B and C is based on systemic administration of recombinant human alpha interferon (r-hIFN-alpha). However, systemic delivery of r-hIFN-alpha is associated with severe side effects, but more importantly, it is effective in only a small percentage of patients. In an effort to maximize I...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4816-4823.2000
更新日期:2000-05-01 00:00:00
abstract::Mice were infected with lymphocytic choriomeningitis virus and injected once 24 h later with a monoclonal antibody directed against gamma interferon. In comparison with controls, the increase of numbers of CD8+ T cells and the generation of virus-specific cytotoxic T lymphocytes in spleens and virus clearance from org...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1521-1526.1996
更新日期:1996-03-01 00:00:00
abstract::The Epstein-Barr virus (EBV) BMRF1 protein is a DNA polymerase processivity factor. We have deleted the BMRF1 open reading frame from the EBV genome and assessed the DeltaBMRF1 EBV phenotype. DeltaBMRF1 viruses were replication deficient, but the wild-type phenotype could be restored by BMRF1 trans-complementation. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.10.5078-5081.2006
更新日期:2006-05-01 00:00:00
abstract::Reovirus encephalitis in mice was used as a model system to investigate astrocyte activation (astrogliosis) following viral infection of the brain. Reovirus infection resulted in astrogliosis, as evidenced by increased expression of glial fibrillary acidic protein (GFAP), and the upregulation of genes that have been p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02027-18
更新日期:2019-05-01 00:00:00
abstract::Attempts to elicit broadly neutralizing antibody responses by human immunodeficiency virus type 1 (HIV-1) vaccine antigens have been met with limited success. To better understand the requirements for cross-neutralization of HIV-1, we have characterized the neutralizing antibody specificities present in the sera of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02749-06
更新日期:2007-06-01 00:00:00
abstract::Elite controllers (EC) of human immunodeficiency virus type 1 (HIV-1) maintain viremia below the limit of detection without antiretroviral treatment. Virus-specific cytotoxic CD8(+) T lymphocytes are believed to play a crucial role in viral containment, but the degree of immune imprinting and compensatory mutations in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02459-08
更新日期:2009-04-01 00:00:00
abstract::The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3645-3647.1987
更新日期:1987-11-01 00:00:00