Abstract:
:Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Other nucleoside triphosphates could be substituted for ATP, but none of the common nucleoside triphosphates was as effective as ATP in promoting in vitro encapsulation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Masker WEdoi
10.1128/JVI.43.1.365-367.1982subject
Has Abstractpub_date
1982-07-01 00:00:00pages
365-7issue
1eissn
0022-538Xissn
1098-5514journal_volume
43pub_type
杂志文章abstract::Vaccinia virus does not grow in Chinese hamster ovary (CHO-K1) cells in the absence of a viral host range factor, cowpox protein CP77. In this study, CP77 was fused to the C terminus of green fluorescence protein (GFP-CP77) and a series of nested deletion mutants of GFP-CP77 was constructed for insertion into a vaccin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00207-06
更新日期:2006-08-01 00:00:00
abstract::We have previously described a nonconditional mutant of avian sarcoma virus (SE21Q1b) which fails to package viral RNA (Gallis et al., Virology 94:146-161, 1979; Linial et al., Cell 15:1371-1381, 1978). Quail cells transformed by SE21Q1b contain normal amounts of intracellular viral mRNA's for src, env, and gag-pol an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.2.450-456.1980
更新日期:1980-11-01 00:00:00
abstract::The assembly of hepatitis C virus (HCV) is poorly understood, largely due to the lack of mammalian cell culture systems that are easily manipulated and produce high titers of virus. This problem is highlighted by the inability of the recently established HCV replicon systems to support HCV capsid assembly despite high...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9257-9269.2004
更新日期:2004-09-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) vpr inhibits the replication of tumor cell lines and peripheral blood mononuclear cells. Here it is demonstrated that expression of vpr, either in the context of a provirus or from an independent genetic element, induces a discrete cell cycle arrest, with cells containing 4N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
更新日期:1995-11-01 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::The promoter P7535 of human papillomavirus type 8 and the promoter P7185 of bovine papillomavirus type 1 are negatively regulated by viral E2 proteins via the promoter proximal binding sites P2 and BS1, respectively. Mutations of these E2 binding sites can reduce basal promoter activity. This suggests binding of a tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.8029-8034.1997
更新日期:1997-10-01 00:00:00
abstract::Innate immunity provides an immediate defense against infection after host cells sense danger signals from microbes. Endoplasmic reticulum (ER) stress arises from accumulation of misfolded/unfolded proteins when protein load overwhelms the ER folding capacity, which activates the unfolded protein response (UPR) to res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01690-17
更新日期:2017-12-14 00:00:00
abstract::A novel circular DNA virus sequence is reported from grapevine. The corresponding genomic organization, coding potential, and conserved origin of replication are similar to those of members of the family Geminiviridae, but the genome of 3,206 nucleotides is 4% larger than the largest reported geminiviral genome and sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00943-12
更新日期:2012-07-01 00:00:00
abstract::Kinetic curves for neutralization of adenovirus type 2 with anti-hexon serum revealed no lag periods even when the serum was highly diluted or when the temperature was lowered to 4 degrees C, thus indicating a single-hit mechanism. Multiplicity curves determined with anti-hexon serum displayed a linear correlation bet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.7.2321-2328.1988
更新日期:1988-07-01 00:00:00
abstract::Two of 25 healthy pet sooty mangabey (SM) monkeys (Cercocebus atys) living in West Africa were seropositive by immunoblot when surveyed for antibody to simian immunodeficiency virus of macaques (SIVmac). SIVsmLIB1 was isolated from one of the pet sooty mangabeys. Nucleotide sequence data showed that this isolate is a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4480-4485.1991
更新日期:1991-08-01 00:00:00
abstract::Nef, an accessory protein of human and simian immunodeficiency viruses, is a critical determinant of pathogenesis that promotes the progression from infection to AIDS. The pathogenic effects of Nef are in large part dependent on its ability to downregulate the macrophage and T-cell coreceptor, CD4. It has been propose...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02725-06
更新日期:2007-04-01 00:00:00
abstract::Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A gene can be introduced by either infection or transfection, and either of the two major E1A proteins, 289R or 243R, can in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1236-1244.1991
更新日期:1991-03-01 00:00:00
abstract::The sequence of the human immunodeficiency virus type 2 circle junction was determined. The most common sequence found between the conserved CA and TG dinucleotides at the ends of the integrated provirus was five bases long (GGTAC). This suggests that the integration of human immunodeficiency virus type 2 DNA is accom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3906-3910.1991
更新日期:1991-07-01 00:00:00
abstract::Mutations in several subgenomic regions of hepatitis C virus (HCV) have been implicated in influencing the response to interferon (IFN) therapy. Sequences within HCV NS5A (PKR binding domain [PKRBD], IFN sensitivity-determining region [ISDR], and variable region 3 [V3]) were analyzed for the pretreatment serum samples...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02231-07
更新日期:2008-07-01 00:00:00
abstract::Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the lef...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.320-329.1998
更新日期:1998-01-01 00:00:00
abstract::By using a series of deletion mutants, we have shown that the stimulation of fibroblast growth by E26 requires the cooperation of the two oncogenes, v-myb and v-ets, fused in the nuclear viral product. Of the two DNA-binding domains, only one must be present to promote anchorage-independent growth, whereas that of v-m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3928-3931.1991
更新日期:1991-07-01 00:00:00
abstract::Due to increasing concerns about human infection by various H7 influenza viruses, including recent H7N9 viruses, we evaluated the genetic relationships and cross-protective efficacies of three different Eurasian H7 avian influenza viruses. Phylogenic and molecular analyses revealed that recent Eurasian H7 viruses can ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02259-16
更新日期:2017-05-12 00:00:00
abstract::Several independent cell lines transformed by simian virus 40 carry a species of viral RNA of 900,000 to 1,000,000 daltons. A viral RNA species of similar size is found early in the lytic cycle. Late in the viral lytic cycle, two prominent viral RNA species of about 600,000 and 900,000 daltons are seen. The larger lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.6.1263-1273.1974
更新日期:1974-06-01 00:00:00
abstract::Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3330-3338.1992
更新日期:1992-06-01 00:00:00
abstract::Differentiating infected from vaccinated animals (DIVA) strategies have been central enabling techniques in several successful viral disease elimination programs. However, owing to their long and uncertain development process, no DIVA-compatible vaccines are available for many important diseases. We report herein a ne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01573-19
更新日期:2020-02-28 00:00:00
abstract::The activity of uracil-DNA glycosylase in Escherichia coli decreases dramatically to less than 10% of its original level after infection of the cells by phage T5. Phage-induced protein synthesis is required for this inhibition to occur, and the inhibition is induced by a mutant capable of injecting only the first 8% o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.535-538.1980
更新日期:1980-01-01 00:00:00
abstract::Following subcutaneous inoculation of newborn Wistar-Furth rats with human adenovirus type 9 (Ad9), 16 of 16 female and 0 of 11 male rats developed mammary tumors. Tumor-positive animals usually developed tumors in multiple glands. Histopathological analyses indicated that three general categories of tumor could be id...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3192-3202.1991
更新日期:1991-06-01 00:00:00
abstract::Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1248-1252.1987
更新日期:1987-04-01 00:00:00
abstract::The major histocompatibility complex (MHC) class I region of humans, chimpanzees (Pan troglodytes), and bonobos (Pan paniscus) is highly similar, and orthologues of HLA-A, -B, and -C are present in both Pan species. Based on functional characteristics, the different HLA-A allotypes are classified into different supert...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01960-19
更新日期:2020-04-16 00:00:00
abstract::The human immunodeficiency virus rev gene product regulates the expression of viral structural genes. It was recently shown that Rev regulates the export of viral structural mRNAs from the nucleus to the cytoplasm. Analysis of Rev subcellular localization reveals marked accumulation in the nucleolus, suggesting a role...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.881-885.1990
更新日期:1990-02-01 00:00:00
abstract::The DNA of lymphomas from 12 AKXD recombinant inbred mouse strains was analyzed to determine the presence of somatically acquired ecotropic and mink cell focus-forming proviruses. Mink cell focus-forming proviruses were associated primarily with T-cell lymphomas, whereas ecotropic proviruses were associated with lymph...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2929-2933.1987
更新日期:1987-09-01 00:00:00
abstract::Lassa fever (LF) is a potentially lethal human disease that is caused by the arenavirus Lassa virus (LASV). Annually, around 300,000 infections with up to 10,000 deaths occur in regions of Lassa fever endemicity in West Africa. Here we demonstrate that mice lacking a functional STAT1 pathway are highly susceptible to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01433-13
更新日期:2013-10-01 00:00:00
abstract::Previous studies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of intermediate and late cDNA products. To further characterize this effect, we evaluated the topology of the 5' end of the HIV-1 RNA genome during early...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01377-17
更新日期:2017-11-14 00:00:00
abstract::BCX 2798 (4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid) effectively inhibited the activities of the hemagglutinin-neuraminidase (HN) of human parainfluenza viruses (hPIV) in vitro and protected mice from lethal infection with a recombinant Sendai virus whose HN ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00474-08
更新日期:2008-09-01 00:00:00
abstract::The primary pathophysiologic finding of the viral disease known as Korean hemorrhagic fever, the etiological agent of which is Hantaan virus (HTV), is vascular instability. To investigate whether HTV was able to infect cells derived from human vascular tissue and alter their behavior, we infected in vitro primary adul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5929-5936.1992
更新日期:1992-10-01 00:00:00