Abstract:
:Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by the overlapping ORF E2 unchanged. Neither mutation interfered with the abilities of the mutants to efficiently induce focus formation, induce growth in soft agarose, or transactivate an inducible bovine papillomavirus type 1 enhancer. Also, neither mutation prevented establishment of the viral DNA as an extrachromosomal plasmid in transformed cells. These results suggest that ORF E4 proteins are not required for these biological activities, and they are consistent with the observation of others (J. Doorbar, D. Campbell, R. J. A. Grand, and P. H. Gallimore, EMBO J. 5:355-362, 1986) that the ORF E4 protein of a human papillomavirus is associated with late gene expression during papilloma formation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Neary K,Horwitz BH,DiMaio Ddoi
10.1128/JVI.61.4.1248-1252.1987subject
Has Abstractpub_date
1987-04-01 00:00:00pages
1248-52issue
4eissn
0022-538Xissn
1098-5514journal_volume
61pub_type
杂志文章abstract::A major challenge for the development of an effective HIV vaccine is to elicit neutralizing antibodies against a broad array of primary isolates. Monomeric gp120-based vaccine approaches have not been successful in inducing this type of response, prompting a number of approaches designed to recreate the native glycopr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14804-14814.2005
更新日期:2005-12-01 00:00:00
abstract::Systemic application of first-generation adenovirus induces pathogenic effects in the liver. To begin unraveling the mechanisms underlying early liver toxicity after adenovirus infusion, particularly the role of macrophage activation and expression of viral genes in transduced target cells, first-generation adenovirus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8798-8807.1997
更新日期:1997-11-01 00:00:00
abstract::Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10448-10455.2003
更新日期:2003-10-01 00:00:00
abstract::The polypeptides of three paramyxoviruses (simian virus 5, Newcastle disease virus, and Sendai virus) were separated by polyacrylamide gel electrophoresis. Glycoproteins were identified by the use of radioactive glucosamine as a carbohydrate precursor. The protein patterns reveal similarities among the three viruses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.1.47-52.1971
更新日期:1971-01-01 00:00:00
abstract::Inoculation of the leafhopper cell line AC-20 with wound tumor virus resulted in a productive noncytopathic infection with no detectable alteration of cellular protein synthesis. Virus-specific polypeptide synthesis, detectable by 8 h postinoculation, increased in a linear fashion, reaching a peak (approximately 10 to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.620-624.1985
更新日期:1985-11-01 00:00:00
abstract::Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), that is clinically indistinguishable from foot-and-mouth disease (FMD), in pigs. Notably, SVA RNA has been detected in lymphoid tissues of infected animals several weeks following resolution of the clinical disease, suggesting that the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00819-19
更新日期:2019-10-15 00:00:00
abstract::To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.886-893.1993
更新日期:1993-02-01 00:00:00
abstract::Determinants of glycosylation site usage were explored by using the hemagglutinin-neuraminidase (HN) glycoprotein of the paramyxovirus Newcastle disease virus. The amino acid sequence of the HN protein, a type II glycoprotein, has six N-linked glycosylation addition sites, G1 to G6, two of which, G5 and G6, are not us...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3083-3089.1997
更新日期:1997-04-01 00:00:00
abstract::In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
abstract::Precursor polyproteins containing translational products of the gag gene of Moloney murine leukemia virus were purified by gel electrophoresis and cleaved into large fragments by hydroxylamine, mild acid hydrolysis, or cyanogen bromide. The hydroxylamine cleavage method (specific for asparagine-glycine bonds) was adap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.3.841-859.1983
更新日期:1983-06-01 00:00:00
abstract::Type I interferons induce a complex transcriptional program that leads to a generalized antiviral response against a large panel of viruses, including human immunodeficiency virus type 1 (HIV-1). However, despite the fact that interferons negatively regulate HIV-1 ex vivo, a chronic interferon state is linked to the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02553-12
更新日期:2013-03-01 00:00:00
abstract::Vaccinia virus (VACV) stimulates long-term immunity against highly pathogenic orthopoxvirus infection of humans (smallpox) and mice (mousepox [ectromelia virus {ECTV}]) despite the lack of a natural host-pathogen relationship with either of these species. Previous research revealed that VACV is able to induce polyfunc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00038-12
更新日期:2012-07-01 00:00:00
abstract::The Duplan strain of murine leukemia virus induces murine AIDS in C57BL/6 mice. When spleen cells from C57BL/6 mice infected with the virus were transplanted into nude mice, subcutaneous solid tumors at the transplanted sites were formed and splenomegaly and lymphadenopathy were induced. These transplantable cells wer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5691-5695.1992
更新日期:1992-09-01 00:00:00
abstract::The envelope glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) is posttranslationally cleaved into two subunits. We show here that this endoproteolytic processing is not required for transport to the cell surface but is essential for LCMV GP to mediate infectivity of pseudotyped retroviral vectors. By sys...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.2866-2872.2003
更新日期:2003-03-01 00:00:00
abstract::The functions of the two rotavirus glycoproteins were investigated by using tunicamycin and a variant of SA11 rotavirus having nonglycosylated VP7. Results showed that glycosylation of VP7 is not required for normal viral morphogenesis and infectivity and suggested that the nonstructural glycoprotein is involved in as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.270-274.1983
更新日期:1983-04-01 00:00:00
abstract:UNLABELLED:Virus-like particles (VLPs) built on the Newcastle disease virus (NDV) core proteins, NP and M, and containing two chimeric proteins, F/F and H/G, composed of respiratory syncytial virus (RSV) fusion protein (F) and glycoprotein (G) ectodomains fused to the transmembrane and cytoplasmic domains of the NDV F ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00384-15
更新日期:2015-07-01 00:00:00
abstract::HeLa cells infected with human rhinovirus type 2 synthesize a mixture of single-and double-stranded ribonucleic acid (RNA). The RNA synthesized by the membrane-bound RNA polymerase complex in vitro is also a mixture of single- and double-stranded RNA, whereas the deoxycholate-treated RNA polymerase complex synthesized...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.1.93-98.1972
更新日期:1972-07-01 00:00:00
abstract:UNLABELLED:Gammaherpesviruses establish persistent, systemic infections and cause cancers. Murid herpesvirus 4 (MuHV-4) provides a unique window into the early events of host colonization. It spreads via lymph nodes. While dendritic cells (DC) pass MuHV-4 to lymph node B cells, subcapsular sinus macrophages (SSM), whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01108-16
更新日期:2016-09-29 00:00:00
abstract::Data showing that an apoptotic reaction (the exit into the cytoplasm and nucleolytic internucleosomal degradation of chromosomal DNA, compaction and fragmentation of chromatin, cellular shrinkage, and cytoplasmic blebbing) developed in a subline of HeLa-S3 cells upon nonpermissive poliovirus infection with either a gu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1181-1189.1995
更新日期:1995-02-01 00:00:00
abstract::Coevolution of virus and host is a process that emerges in persistent virus infections. Here we studied the coevolutionary development of coxsackievirus B3 (CVB3) and cardiac myocytes representing the major target cells of CVB3 in the heart in a newly established persistently CVB3-infected murine cardiac myocyte cell ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00621-11
更新日期:2011-12-01 00:00:00
abstract::The involvement of two bacteriophage T4 gene products in the initiation of T4 tail tube and sheath polymerization on mature baseplates has been studied by radioautography of acrylamide gels of various partially completed tail structures. The products of genes 48 and 54 (P48[the nomenclature P48 refers to the protein p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1669-1677.1975
更新日期:1975-12-01 00:00:00
abstract::Hepatitis C virus (HCV) is a single-stranded positive-sense RNA hepatotropic virus. Despite cellular defenses, HCV is able to replicate in hepatocytes and to establish a chronic infection that could lead to severe complications and hepatocellular carcinoma. An important player in subverting the host response to HCV in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00880-17
更新日期:2017-08-24 00:00:00
abstract::Cytomegalovirus (CMV) retinitis is an important ocular complication in human immunodeficiency virus-infected individuals and the leading cause of blindness in those not undergoing highly active antiretroviral therapy. Murine CMV (MCMV) infection of mice has been shown to be a useful small-animal model for the study of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9165-9175.2002
更新日期:2002-09-01 00:00:00
abstract::We have previously described several human immunodeficiency virus type 1 (HIV-1) mutants that are characterized by an excessive-RNA-splicing phenotype and reduced virus particle production. In one of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice donor site. This splice sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02295-07
更新日期:2008-02-01 00:00:00
abstract::Bluetongue virus (BTV), an insect-vectored emerging pathogen of both wild ruminants and livestock, has had a severe economic impact in agriculture in many parts of the world. The investigation of BTV replication and pathogenesis has been hampered by the lack of a reverse genetics system. Recovery of infectious BTV is ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-08
更新日期:2008-09-01 00:00:00
abstract::Adenovirus infection and expression of E1A induces both proliferation and apoptosis, the latter of which is blocked by the adenovirus Bcl-2 homologue E1B 19K. The mechanism of apoptosis induction and the role that it plays in productive infection are not known. Unlike apoptosis mediated by death receptors, infection w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4547-4558.2002
更新日期:2002-05-01 00:00:00
abstract::The VP24 protein plays an essential, albeit poorly understood role in the filovirus life cycle. VP24 is only 30% identical between Marburg virus and the ebolaviruses. Furthermore, VP24 from the ebolaviruses is immunosuppressive, while that of Marburg virus is not. The crystal structure of Marburg virus VP24, presented...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03565-13
更新日期:2014-05-01 00:00:00
abstract::Viruses display a wide range of genomic profiles and, consequently, a variety of gene expression strategies. Specific sequences associated with transcriptional processes have been described in viruses, and putative promoter motifs have been elucidated for some nucleocytoplasmic large DNA viruses (NCLDV). Among NCLDV, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01088-17
更新日期:2017-10-13 00:00:00
abstract::The insertion of a particular phi X DNA sequence in the plasmid pACYC177 strongly decreased the capacity of Escherichia coli cells containing such a plasmid to propagate bacteriophage phi X174. The smallest DNA sequence tested that showed the effect was the HindII fragment R4. This fragment does not code for a complet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.1-11.1982
更新日期:1982-04-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen associated with chronic liver disease. Recently, based on a genotype 2a isolate, tissue culture systems supporting complete replication and infectious virus production have been developed. In this study, we used cell culture-produced infectious HCV to analyze the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1734-1741.2006
更新日期:2006-02-01 00:00:00