Mutational analysis of open reading frame E4 of bovine papillomavirus type 1.

Abstract:

:Open reading frame (ORF) E4 is a 353-base-pair ORF of bovine papillomavirus type 1. To determine the biological activities of this ORF in mouse C127 cells, we analyzed the effects of two constructed mutations which are predicted to prevent synthesis of ORF E4 proteins while leaving the amino acid sequence encoded by the overlapping ORF E2 unchanged. Neither mutation interfered with the abilities of the mutants to efficiently induce focus formation, induce growth in soft agarose, or transactivate an inducible bovine papillomavirus type 1 enhancer. Also, neither mutation prevented establishment of the viral DNA as an extrachromosomal plasmid in transformed cells. These results suggest that ORF E4 proteins are not required for these biological activities, and they are consistent with the observation of others (J. Doorbar, D. Campbell, R. J. A. Grand, and P. H. Gallimore, EMBO J. 5:355-362, 1986) that the ORF E4 protein of a human papillomavirus is associated with late gene expression during papilloma formation.

journal_name

J Virol

journal_title

Journal of virology

authors

Neary K,Horwitz BH,DiMaio D

doi

10.1128/JVI.61.4.1248-1252.1987

subject

Has Abstract

pub_date

1987-04-01 00:00:00

pages

1248-52

issue

4

eissn

0022-538X

issn

1098-5514

journal_volume

61

pub_type

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