Maintenance of an intact human immunodeficiency virus type 1 vpr gene following mother-to-infant transmission.


:The vpr sequences from six human immunodeficiency virus type 1 (HIV-1)-infected mother-infant pairs following perinatal transmission were analyzed. We found that 153 of the 166 clones analyzed from uncultured peripheral blood mononuclear cell DNA samples showed a 92.17% frequency of intact vpr open reading frames. There was a low degree of heterogeneity of vpr genes within mothers, within infants, and between epidemiologically linked mother-infant pairs. The distances between vpr sequences were greater in epidemiologically unlinked individuals than in epidemiologically linked mother-infant pairs. Moreover, the infants' sequences displayed patterns similar to those seen in their mothers. The functional domains essential for Vpr activity, including virion incorporation, nuclear import, and cell cycle arrest and differentiation were highly conserved in most of the sequences. Phylogenetic analyses of 166 mother-infant pairs and 195 other available vpr sequences from HIV databases formed distinct clusters for each mother-infant pair and for other vpr sequences and grouped the six mother-infant pairs' sequences with subtype B sequences. A high degree of conservation of intact and functional vpr supports the notion that vpr plays an important role in HIV-1 infection and replication in mother-infant isolates that are involved in perinatal transmission.


J Virol


Journal of virology


Yedavalli VR,Chappey C,Ahmad N




Has Abstract


1998-08-01 00:00:00












  • Disrupting surfaces of nef required for downregulation of CD4 and for enhancement of virion infectivity attenuates simian immunodeficiency virus replication in vivo.

    abstract::The multifunctional simian and human immunodeficiency virus (SIV and HIV) Nef proteins are important for virulence. We studied the importance of selected Nef functions using an SIV Nef with mutations in two regions that are required for CD4 downregulation. This Nef mutant is defective for downregulating CD4 and, in ad...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Iafrate AJ,Carl S,Bronson S,Stahl-Hennig C,Swigut T,Skowronski J,Kirchhoff F

    更新日期:2000-11-01 00:00:00

  • Rapid viral decay in simian immunodeficiency virus-infected macaques receiving quadruple antiretroviral therapy.

    abstract::The viral dynamics in human immunodeficiency virus type 1 (HIV-1) infection have been studied extensively using mathematical modeling, but data from other primate lentivirus systems are scarce. This study was initiated to increase the understanding of the differences and similarities between the different primate lent...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Brandin E,Thorstensson R,Bonhoeffer S,Albert J

    更新日期:2006-10-01 00:00:00

  • Selectable recombinant herpesvirus saimiri is capable of persisting in a human T-cell line.

    abstract::Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cell...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Grassmann R,Fleckenstein B

    更新日期:1989-04-01 00:00:00

  • Synergistic neutralization of simian-human immunodeficiency virus SHIV-vpu+ by triple and quadruple combinations of human monoclonal antibodies and high-titer anti-human immunodeficiency virus type 1 immunoglobulins.

    abstract::We have tested triple and quadruple combinations of human monoclonal antibodies (MAbs), which are directed against various epitopes on human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins, and a high-titer anti-HIV-1 human immunoglobulin (HIVIG) preparation for their abilities to neutralize a chimeric si...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Li A,Katinger H,Posner MR,Cavacini L,Zolla-Pazner S,Gorny MK,Sodroski J,Chou TC,Baba TW,Ruprecht RM

    更新日期:1998-04-01 00:00:00

  • Poorly lytic bacteriophage from Dactylosporangium thailandensis (Actinomycetales).

    abstract::Dactylosporangiophage A1 has a polygonal head (75 nm) with spherical capsomeres (3 nm) and a noncontractile tail (200 by 10 nm) with cross-striations which is terminated with at least three prongs which are used for attachment. It contains double-stranded deoxyribonucleic acid and produces very little lysis. Intracell...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Higgins ML,Lechevalier MP

    更新日期:1969-02-01 00:00:00

  • Chromosomal localization of the Moloney sarcoma virus mouse cellular (c-mos) sequence.

    abstract::The Moloney sarcoma virus-specific onc gene, referred to as v-mos, was used as probe to hybridize to restricted DNAs from various mouse-Chinese hamster hybrid cell lines. These hybrid cells contain, in addition to all of the Chinese hamster chromosomes, various numbers (less than a full complement) of mouse chromosome...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Swan D,Oskarsson M,Keithley D,Ruddle FH,D'Eustachio P,Vande Woude GF

    更新日期:1982-11-01 00:00:00

  • Autonomous parvovirus LuIII encapsidates equal amounts of plus and minus DNA strands.

    abstract::Autonomous parvoviruses are thought to uniquely encapsidate single-stranded DNA of minus polarity. In contrast, the defective adeno-associated viruses separately encapsidate equal amounts of plus and minus DNA strands. We reexamined the uniqueness of minus strand encapsidation for the autonomous parvoviruses. Although...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bates RC,Snyder CE,Banerjee PT,Mitra S

    更新日期:1984-02-01 00:00:00

  • Naive T-cell depletion related to infection by X4 human immunodeficiency virus type 1 in poor immunological responders to highly active antiretroviral therapy.

    abstract::The reasons for poor CD4+ T-cell recovery in some human immunodeficiency virus (HIV)-infected subjects despite effective highly active antiretroviral therapy (HAART) remain unclear. We recently reported that CXCR4-using (X4) HIV-1 could be gradually selected in cellular reservoirs during sustained HAART. Because of th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Delobel P,Nugeyre MT,Cazabat M,Sandres-Sauné K,Pasquier C,Cuzin L,Marchou B,Massip P,Cheynier R,Barré-Sinoussi F,Izopet J,Israël N

    更新日期:2006-10-01 00:00:00

  • Identification of amino acid residues in CD81 critical for interaction with hepatitis C virus envelope glycoprotein E2.

    abstract::Human CD81 has been previously identified as the putative receptor for the hepatitis C virus envelope glycoprotein E2. The large extracellular loop (LEL) of human CD81 differs in four amino acid residues from that of the African green monkey (AGM), which does not bind E2. We mutated each of the four positions in human...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Higginbottom A,Quinn ER,Kuo CC,Flint M,Wilson LH,Bianchi E,Nicosia A,Monk PN,McKeating JA,Levy S

    更新日期:2000-04-01 00:00:00

  • Ineffectual targeting of HIV-1 Nef by cytotoxic T lymphocytes in acute infection results in no functional impairment or viremia reduction.

    abstract::The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef is heavily targeted by CD8(+) T lymphocytes (CTLs) during acute infection and therefore is included in many candidate vaccines. We investigated whether CTL targeting of Nef during acute infection contributes to immune control by disrupting the funct...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: De La Cruz J,Vollbrecht T,Frohnen P,Ng HL,Daar ES,Yang OO,Lewis MJ

    更新日期:2014-07-01 00:00:00

  • Influenza A (H15N4) virus isolation in Western Siberia, Russia.

    abstract::The rarely identified influenza A viruses of the H15 hemagglutinin subtype have been isolated exclusively in Australia. Here we report the isolation of an H15N4 influenza A virus (A/teal/Chany/7119/2008) in Western Siberia, Russia. Phylogenetic analysis demonstrated that the internal genes of the A/teal/Chany/7119/200...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sivay MV,Baranovich T,Marchenko VY,Sharshov KA,Govorkova EA,Shestopalov AM,Webby RJ

    更新日期:2013-03-01 00:00:00

  • Assignment of the large oligonucleotides of vesicular stomatitis virus to the N, NS, M, G, and L genes and oligonucleotide gene ordering within the L gene.

    abstract::Analyses of prototype vesicular stomatitis (VSV, Indiana serotype) mRNA-32P-labeled viral RNA duplexes have established the assignments of 65 of the 72 large oligonucleotides that are recovered by two-dimensional electrophoresis of RNase T1 digests of the viral RNA. Fifty of the oligonucleotides are recovered in the L...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Clewley JP,Bishop DH

    更新日期:1979-04-01 00:00:00

  • Zinc finger domain of murine leukemia virus nucleocapsid protein enhances the rate of viral DNA synthesis in vivo.

    abstract::In vitro studies have indicated that retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor template. Increasing the rate of DNA synthesis is expected to reduce the frequency of RT template switching, whereas annealing the nascen...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zhang WH,Hwang CK,Hu WS,Gorelick RJ,Pathak VK

    更新日期:2002-08-01 00:00:00

  • Expression of polyomavirus large T antigen by using a baculovirus vector.

    abstract::A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect c...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rice WC,Lorimer HE,Prives C,Miller LK

    更新日期:1987-05-01 00:00:00

  • Recombinant human Metapneumovirus lacking the small hydrophobic SH and/or attachment G glycoprotein: deletion of G yields a promising vaccine candidate.

    abstract::Human metapneumovirus (HMPV) has recently been identified as a significant cause of serious respiratory tract disease in humans. In particular, the emerging information on the contribution of HMPV to pediatric respiratory tract disease suggests that it will be important to develop a vaccine against this virus for use ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Biacchesi S,Skiadopoulos MH,Yang L,Lamirande EW,Tran KC,Murphy BR,Collins PL,Buchholz UJ

    更新日期:2004-12-01 00:00:00

  • SATB1-binding sequences and Alu-like motifs define a unique chromatin context in the vicinity of human immunodeficiency virus type 1 integration sites.

    abstract::Retroviral integration has recently been shown to be nonrandom, favoring transcriptionally active regions of chromatin. However, the mechanism for integration site selection by retroviruses is not clear. We show here the occurrence of Alu-like motifs in the sequences flanking the reported viral integration sites that ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kumar PP,Mehta S,Purbey PK,Notani D,Jayani RS,Purohit HJ,Raje DV,Ravi DS,Bhonde RR,Mitra D,Galande S

    更新日期:2007-06-01 00:00:00

  • Nipah virus V and W proteins have a common STAT1-binding domain yet inhibit STAT1 activation from the cytoplasmic and nuclear compartments, respectively.

    abstract::In previous reports it was demonstrated that the Nipah virus V and W proteins have interferon (IFN) antagonist activity due to their ability to block signaling from the IFN-alpha/beta receptor (J. J. Rodriguez, J. P. Parisien, and C. M. Horvath, J. Virol. 76:11476-11483, 2002; M. S. Park et al., J. Virol. 77:1501-1511...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Shaw ML,García-Sastre A,Palese P,Basler CF

    更新日期:2004-06-01 00:00:00

  • Phenotypic characterization of insertion mutants of the human immunodeficiency virus type 1 Gag precursor expressed in recombinant baculovirus-infected cells.

    abstract::A panel of 28 insertion mutants of the human immunodeficiency virus type 1 (HIV-1) Gag precursor (Pr55Gag) was constructed by linker-insertion mutagenesis and expressed in recombinant baculovirus-infected insect cells. One set of 14 mutants carried the normal N-myristylation signal; the other set constituted their non...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Chazal N,Carrière C,Gay B,Boulanger P

    更新日期:1994-01-01 00:00:00

  • Antiviral activity of polyacrylic and polymethacrylic acids. II. Mode of action in vivo.

    abstract::A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: De Somer P,De Clercq E,Billiau A,Schonne E,Claesen M

    更新日期:1968-09-01 00:00:00

  • Interactions of murine leukemia virus core components: characterization of reverse transcriptase packaged in the absence of 70S genomic RNA.

    abstract::Virions produced by cells in the presence of actinomycin D (Act D virions) contain reverse transcriptase but are deficient in 70S genomic RNA. To assess the role of genomic RNA in encapsidation of a functional reverse transcriptase and to study the interaction of the enzyme and its template in the cores of intact viri...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gerwin BI,Levin JG

    更新日期:1977-11-01 00:00:00

  • In vivo genetic variability of the human immunodeficiency virus type 2 V3 region.

    abstract::The principal neutralizing epitope of the human immunodeficiency virus type 1 (HIV-1) lies between two invariant cysteines in the third variable region (V3) of the viral envelope (gp120), and its amino acid sequence varies among different HIV-1 isolates. HIV-2 carries an analogous amino acid sequence between two cyste...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Boeri E,Giri A,Lillo F,Ferrari G,Varnier OE,Ferro A,Sabbatani S,Saxinger WC,Franchini G

    更新日期:1992-07-01 00:00:00

  • Effect of vesicular stomatitis virus infection on the histocompatibility antigen of L cells.

    abstract::When mouse L cells are infected for 22 hr with vesicular stomatitis virus (VSV), a ribonucleic acid-containing enveloped virus, greater than 70% of the major histocompatibility antigen (H-2), is no longer detectable by the method of inhibition of immune cytolysis. Infected cells prelabeled with (14)C-glucosamine also ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hecht TT,Summers DF

    更新日期:1972-10-01 00:00:00

  • Proteins of the group B arbovirus Kunjin.

    abstract::Purified Kunjin virus was disrupted with sodium dodecyl sulfate, urea, and mercaptoethanol or acetic acid. Electrophoresis on 7.5% polyacrylamide gel separated four proteins of high (120,000), intermediate (65,000) and low (18,000 and 13,000) molecular weights. A "core" particle was obtained by degradation of the viri...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Westaway EG,Reedman BM

    更新日期:1969-11-01 00:00:00

  • Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy.

    abstract::We have constructed new murine leukemia virus (MLV)-based vectors (TIN vectors) which, following integration, contain human immunodeficiency virus (HIV) type 1 U3 and R sequences in place of the MLV U3 and R regions. This provides, for the first time, single transcriptional unit retroviral vectors under the control of...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cannon PM,Kim N,Kingsman SM,Kingsman AJ

    更新日期:1996-11-01 00:00:00

  • Induction and maintenance of autonomous flock house virus RNA1 replication.

    abstract::The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Johnson KL,Ball LA

    更新日期:1999-10-01 00:00:00

  • Assembly of the herpes simplex virus capsid: preformed triplexes bind to the nascent capsid.

    abstract::The herpes simplex virus type 1 (HSV-1) capsid is a T=16 icosahedral shell that forms in the nuclei of infected cells. Capsid assembly also occurs in vitro in reaction mixtures created from insect cell extracts containing recombinant baculovirus-expressed HSV-1 capsid proteins. During capsid formation, the major capsi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Spencer JV,Newcomb WW,Thomsen DR,Homa FL,Brown JC

    更新日期:1998-05-01 00:00:00

  • Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5.

    abstract::Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced host cell apoptosis has been established, the underlying molecular mechanism is still unclear. We report here that IBDV viral protein 5 (VP5) is a major apoptosis induce...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Li Z,Wang Y,Xue Y,Li X,Cao H,Zheng SJ

    更新日期:2012-02-01 00:00:00

  • Stress Hormones Epinephrine and Corticosterone Selectively Modulate Herpes Simplex Virus 1 (HSV-1) and HSV-2 Productive Infections in Adult Sympathetic, but Not Sensory, Neurons.

    abstract::Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) infect and establish latency in peripheral neurons, from which they can reactivate to cause recurrent disease throughout the life of the host. Stress is associated with the exacerbation of clinical symptoms and the induction of recurrences in humans and animal models. T...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ives AM,Bertke AS

    更新日期:2017-06-09 00:00:00

  • Human endogenous retrovirus K (HML-2) elements in the plasma of people with lymphoma and breast cancer.

    abstract::Actively replicating endogenous retroviruses entered the human genome millions of years ago and became a stable part of the inherited genetic material. They subsequently acquired multiple mutations, leading to the assumption that these viruses no longer replicate. However, certain human tumor cell lines have been show...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Contreras-Galindo R,Kaplan MH,Leissner P,Verjat T,Ferlenghi I,Bagnoli F,Giusti F,Dosik MH,Hayes DF,Gitlin SD,Markovitz DM

    更新日期:2008-10-01 00:00:00

  • Characterization of the 3' Termini of the RNAs of Cowpea Mosaic Virus.

    abstract::A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Steele KP,Frist RH

    更新日期:1978-05-01 00:00:00