Abstract:
:The cytolytic T-lymphocyte (CTL) response to respiratory infection with equine herpesvirus 1 (EHV-1) in CBA (H-2(k)) mice was investigated. Intranasal (i.n.) inoculation of mice with the attenuated EHV-1 strain KyA resulted in the generation of a primary virus-specific CTL response in the draining mediastinal lymph nodes 5 days following infection. EHV-1-specific CTL could be restimulated from the spleen up to 26 weeks after the resolution of infection, indicating that a long-lived memory CTL population was generated. Depletion of CD8+ T cells by treatment with antibody and complement prior to assay eliminated CTL activity from both primary and memory populations, indicating that cytolytic activity in this model was mediated by class I major histocompatibility complex-restricted, CD8+ T cells. A single i.n. inoculation with KyA induced protective immunity against infection with the pathogenic EHV-1 strain, RacL11. The adoptive transfer of splenocytes from KyA-immune donors into sublethally irradiated recipients resulted in a greater than 250-fold reduction in RacL11 in the lung. The elimination of both CD4+ and CD8+ T cells from the transferred cells abrogated clearance of RacL11, while the selective depletion of either subpopulation alone had little effect. These results suggested that both lymphocyte subpopulations contribute to viral clearance, with either subpopulation alone being sufficient.
journal_name
J Viroljournal_title
Journal of virologyauthors
Smith PM,Zhang Y,Jennings SR,O'Callaghan DJdoi
10.1128/JVI.72.7.5366-5372.1998subject
Has Abstractpub_date
1998-07-01 00:00:00pages
5366-72issue
7eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::JC virus (JCV), along with other members of the polyomavirus family, encodes a class of highly conserved proteins, T antigens, that are capable of inducing aneuploidy in cultured cells. We have previously isolated T-antigen DNA variants of JCV from both colon cancer tissues and the corresponding nonneoplastic gastroin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1996-2001.2001
更新日期:2001-02-01 00:00:00
abstract::Recent environmental and metagenomic studies have considerably increased the repertoire of archaeal viruses and suggested that they play important roles in nutrient cycling in the biosphere. However, very little is known about how they regulate their life cycles and interact with their hosts. Here, we report that the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00841-20
更新日期:2020-07-30 00:00:00
abstract::We investigated the use of infectious cDNA for the production of poliovirus type 1-type 3 recombinants. One such recombinant virus was produced, but a second construct involving the transfer of part of the capsid protein region was not infectious. Our results suggest that the approach may prove valuable but that not a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1187-1190.1986
更新日期:1986-03-01 00:00:00
abstract::To date, no vaccine that is safe and effective against herpes simplex virus 2 (HSV-2) disease has been licensed. In this study, we evaluated a DNA prime-formalin-inactivated-HSV-2 (FI-HSV2) boost vaccine approach in the guinea pig model of acute and recurrent HSV-2 genital disease. Five groups of guinea pigs were immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02521-10
更新日期:2011-04-01 00:00:00
abstract::During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.988-1001.1994
更新日期:1994-02-01 00:00:00
abstract::Interleukin-1 receptor antagonist (IL-1ra) gene polymorphisms in 83 human immunodeficiency virus (HIV)-seropositive women were evaluated. Fourteen of the subjects (16.9%) were homozygous for IL-1ra allele 2 (IL-1RN*2). These women had a lower median level of HIV RNA than did women homozygous for allele 1 (IL-1RN*1) (P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
abstract::The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.995-998.2004
更新日期:2004-01-01 00:00:00
abstract::Proteasome inhibitors (e.g., bortezomib, MG132) are known to enhance adeno-associated virus (AAV) transduction; however, whether this results from pleotropic proteasome inhibition or off-target serine and/or cysteine protease inhibition remains unresolved. Here, we examined recombinant AAV (rAAV) effects of a new prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01826-13
更新日期:2013-12-01 00:00:00
abstract::V3-glycan-targeting broadly neutralizing antibodies (bNAbs) are a focus of HIV-1 vaccine development. Understanding the viral dynamics that stimulate the development of these antibodies can provide insights for immunogen design. We used a deep-sequencing approach, together with neutralization phenotyping, to investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00828-17
更新日期:2017-08-24 00:00:00
abstract::The pattern of induced protein species of the prototype strain of the parvovirus minute virus of mice was determined in permissive A9 mouse fibroblast cells by high-resolution two-dimensional gel electrophoresis. Identities of the viral proteins in the gels were assigned by probing two-dimensional blots with antisera ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5126-5138.1993
更新日期:1993-09-01 00:00:00
abstract::The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein la...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00870-14
更新日期:2014-09-01 00:00:00
abstract::Reassortant viruses which possessed the hemagglutinin and neuraminidase genes of wild-type human influenza A viruses and the remaining six RNA segments (internal genes) of the avian A/Pintail/Alberta/119/79 (H4N6) virus were previously found to be attenuated in humans. To study the genetic basis of this attenuation, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2857-2863.1987
更新日期:1987-09-01 00:00:00
abstract::The use of endocytic pathways by viral glycoproteins is thought to play various functions during viral infection. We previously showed in transfection assays that herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is transported from the cell surface back to the trans-Golgi network (TGN) and that two motifs of gB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-07
更新日期:2007-12-01 00:00:00
abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.812-823.1980
更新日期:1980-09-01 00:00:00
abstract::DNA vaccines have recently emerged at the forefront of approaches to harness the immune system in the prevention and treatment of viral infections, as well as the prevention and treatment of cancers. However, these vaccines suffer from limited efficacy since they often fail to produce significant antigen-specific CD8(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01954-09
更新日期:2010-03-01 00:00:00
abstract::Woodchuck hepatitis virus (WHV) mutants with core internal deletions (CID) occur naturally in chronically WHV-infected woodchucks, as do hepatitis B virus mutants in humans. We studied the replication of WHV deletion mutants in primary woodchuck hepatocyte cultures and in vivo after transmission to naive woodchucks. B...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3811-3818.2001
更新日期:2001-04-01 00:00:00
abstract::BCX 2798 (4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid) effectively inhibited the activities of the hemagglutinin-neuraminidase (HN) of human parainfluenza viruses (hPIV) in vitro and protected mice from lethal infection with a recombinant Sendai virus whose HN ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00474-08
更新日期:2008-09-01 00:00:00
abstract::By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.131-134.1982
更新日期:1982-04-01 00:00:00
abstract:UNLABELLED:Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus in the Secoviridae family. Its RNA1 encodes proteins required for genome replication, whereas RNA2 primarily encodes proteins needed for virion assembly and cell-to-cell movement. However, the function of a 58-kDa protein (P58) e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03301-13
更新日期:2014-03-01 00:00:00
abstract::To study the function of the varicella-zoster virus (VZV) gB cytoplasmic domain during viral infection, we produced a VZV recombinant virus that expresses a truncated form of gB lacking the C-terminal 36 amino acids of its cytoplasmic domain (VZV gB-36). VZV gB-36 replicates in noncomplementing cells and grows at a ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.2.591-599.2002
更新日期:2002-01-01 00:00:00
abstract::Vaccinia virus replication is inhibited by etoposide and mitoxantrone even though poxviruses do not encode the type II topoisomerases that are the specific targets of these drugs. Furthermore, one can isolate drug-resistant virus carrying mutations in the viral DNA ligase and yet the ligase is not known to exhibit sen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02723-07
更新日期:2008-06-01 00:00:00
abstract::The Epstein-Barr virus latent membrane protein (LMP) is an integral membrane protein that is expressed in cells latently infected with the virus. LMP is believed to play an important role in Epstein-Barr virus transformation and has been shown to induce expression of several cellular proteins. We performed a series of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6496-6501.1992
更新日期:1992-11-01 00:00:00
abstract::Budding of retroviruses from cell membranes requires ubiquitination of Gag and recruitment of cellular proteins involved in endosome sorting, including endosome sorting complex required for transport III (ESCRT-III) protein complex and vacuolar protein sorting 4 (VPS4) and its ATPase. In response to infection, a cellu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02610-10
更新日期:2011-07-01 00:00:00
abstract::Primary RNA transcripts from the human immunodeficiency virus type 1 (HIV-1) are processed into mature mRNA by a complex series of splicing events. Viral structural proteins and reverse transcriptase are translated from unspliced or singly spliced transcripts. Proteins which control virus replication, including tat, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3391-3398.1990
更新日期:1990-07-01 00:00:00
abstract::Replication of all positive-strand RNA viruses is intimately associated with membranes. Here we utilize electron tomography and other methods to investigate the remodeling of membranes in poliovirus-infected cells. We found that the viral replication structures previously described as "vesicles" are in fact convoluted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05937-11
更新日期:2012-01-01 00:00:00
abstract::The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.151-160.1975
更新日期:1975-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1)-infected macrophages damage mature neurons in the brain, although their effect on neuronal development has not been clarified. In this study, we show that HIV-1-infected macrophages produce factors that impair the development of neuronal precursor cells and that soluble vira...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02094-07
更新日期:2008-03-01 00:00:00
abstract::The development of vaccines against bluetongue, a prevalent livestock disease, has been focused on surface antigens that induce strong neutralizing antibody responses. Because of their antigenic variability, these vaccines are usually serotype restricted. We now show that a single highly conserved nonstructural protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00938-18
更新日期:2018-07-31 00:00:00
abstract::Varicella-zoster virus (VZV) causes varicella and establishes latency in sensory nerve ganglia, but the characteristics of VZV latency are not well defined. Immunohistochemical detection of the VZV immediate-early 63 (IE63) protein in ganglion neurons has been described, but there are significant discrepancies in esti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02416-09
更新日期:2010-04-01 00:00:00
abstract:UNLABELLED:Venezuelan and western equine encephalitis viruses (VEEV and WEEV; Alphavirus; Togaviridae) are mosquito-borne pathogens causing central nervous system (CNS) disease in humans and equids. Adult CD-1 mice also develop CNS disease after infection with VEEV and WEEV. Adult CD-1 mice infected by the intranasal (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03219-15
更新日期:2016-05-27 00:00:00