Abstract:
:The synthesis of minus-strand RNA was studied in cell cultures infected with the heat-resistant strain of Sindbis virus and with temperature-sensitive (ts) belonging to complementation groups A, B, F, and G, all of which exhibited an RNA-negative (RNA-) phenotype when infection was initiated and maintained at 39 degrees C, the nonpermissive temperature. When infected cultures were shifted from 28 degrees C (the permissive temperature) to 39 degrees C at 3 h postinfection, the synthesis of viral minus-strand RNA ceased in cultures infected with ts mutants of complementation groups B and F, but continued in cultures infected with the parental virus and mutans of complementation groups A and G. In cultures infected with ts11 of complementation group B, the synthesis of viral minus-strand RNA ceased, whereas the synthesis of 42S and 26S plus-strand RNAs continued for at least 5 h after the shift to 39 degrees C. However, when ts11-infected cultures were returned to 28 degrees C 1 h after the shift to 39 degrees C, the synthesis of viral minus-strand RNA resumed, and the rate of viral RNA synthesis increased. The recovery of minus-strand synthesis translation of new proteins. We conclude that at least one viral function is required for alphavirus minus-strand synthesis that is not required for plus-strand synthesis. In cultures infected with ts6 of complementation group F, the syntheses of both viral plus-strand and minus-strand RNAs were drastically reduced after the shift to 39 degrees C. Since ts6 failed to synthesize both plus-strand and minus-strand RNAs after the shift to 39 degrees C, at least one common viral component appears to be required for the synthesis of both minus-strand and plus-strand RNAs.
journal_name
J Viroljournal_title
Journal of virologyauthors
Sawicki DL,Sawicki SG,Keränen S,Kääriäinen Ldoi
10.1128/JVI.39.2.348-358.1981subject
Has Abstractpub_date
1981-08-01 00:00:00pages
348-58issue
2eissn
0022-538Xissn
1098-5514journal_volume
39pub_type
杂志文章abstract:UNLABELLED:JC polyomavirus (JCPyV) infection of immunocompromised individuals results in the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML). The viral capsid of JCPyV is composed primarily of the major capsid protein virus protein 1 (VP1), and pentameric arrangement of VP1 monomers results...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00089-15
更新日期:2015-04-01 00:00:00
abstract::Recently, self-replicating and self-limiting RNA vaccines (RNA replicons) have emerged as an important form of nucleic acid vaccines. Self-replicating RNA eventually causes lysis of transfected cells and does not raise the concern associated with naked DNA vaccines of integration into the host genome. This is particul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2368-2376.2001
更新日期:2001-03-01 00:00:00
abstract::Skin fibroblast cultures were established from eight individuals. These cell cultures, together with WI-38 cells, were examined for susceptibility to transformation by SV40 virus. Four transformation-susceptible cell lines (TS), established from patients with Down's syndrome, were found to be three to four times more ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.3.293-298.1970
更新日期:1970-03-01 00:00:00
abstract::We have found that interleukin-4 (IL-4) abrogated constitutive beta interferon (IFN-beta) synthesis in mouse macrophages. Analysis of endogenous IFN mRNA by reverse transcription-PCR clearly documented the presence of IFN-beta but not of IFN-alpha transcripts in RNA from bone marrow-derived macrophages (BMM). Culture ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6763-6766.1994
更新日期:1994-10-01 00:00:00
abstract::Woodchuck hepatitis virus (WHV) and hepatitis B virus (HBV) are closely similar with respect to genomic organization, host antiviral responses, and pathobiology of the infection. T-cell immunity against viral nucleocapsid (HBcAg or WHcAg) has been shown to play a critical role in viral clearance and protection against...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9068-9076.2001
更新日期:2001-10-01 00:00:00
abstract::Beta interferon (IFN-beta) expression is triggered by double-stranded RNA, a common intermediate in the replication of many viruses including hepatitis C virus (HCV). The recent development of cell culture-grown HCV allowed us to analyze the IFN signaling pathway following virus infection. In this study, we have exami...
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pub_type: 杂志文章
doi:10.1128/JVI.01695-07
更新日期:2007-11-01 00:00:00
abstract::The genetic basis for the distinctive capacity of influenza A/WSN/33 (H0N1) virus (WSN virus) to produce plaques on bovine kidney (MDBK) cells was found to be related to virus neuraminidase. Recombinant viruses that derived only the neuraminidase of WSN virus were capable of producing plaques, whereas recombinant viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.1.170-176.1977
更新日期:1977-10-01 00:00:00
abstract::In the search for effective immunologic interventions to prevent and treat HIV-1 infection, standardized reference reagents are a cost-effective way to maintain robustness and reproducibility among immunological assays. To support planned and ongoing studies where clade C predominates, here we describe three virus pan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00991-17
更新日期:2017-09-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02158-18
更新日期:2019-03-05 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10338-10345.2002
更新日期:2002-10-01 00:00:00
abstract::Vaccinia virus DNA fragments that have been denatured by alkali and then neutralized contain a fraction that rapidly reforms duplex structures. The fraction is enriched by fractionating on hydroxyapatite columns and serves as as substrate for digestion by two restriction endonucleases isolated from Hemophilus parainfl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.227-238.1976
更新日期:1975-01-01 00:00:00
abstract::Nonglucosylated and fragmented T4 DNA shows a gene-specific variation in transformation efficiency. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.397-400.1973
更新日期:1973-08-01 00:00:00
abstract::tsAF8 cells are temperature-sensitive (ts) mutants of BHK-21 cells that arrest at the nonpermissive temperature in the G1 phase of the cell cycle. When made quiescent by serum restriction, they can be stimulated to enter the S phase by 10% serum at 34 degrees C, but not at 40.6 degrees C. Infection by adenovirus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.982-986.1981
更新日期:1981-06-01 00:00:00
abstract::The filoviruses Ebola Zaire virus and Marburg virus are believed to infect target cells through endocytic vesicles, but the details of this pathway are unknown. We used a pseudotyping strategy to investigate the cell biology of filovirus entry. We observed that specific inhibitors of the caveola system, including chol...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5266-5270.2002
更新日期:2002-05-01 00:00:00
abstract::We characterized a number of important features of the structure of the cohesive overlap region of the DNA genome of duck hepatitis B virus. The 5'-terminal nucleotide of minus-strand DNA was localized to nucleotide 2537, a G residue within the 12-base repeat sequence DR1. This G residue was shown to be the site of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
abstract::ZEBRA is an Epstein-Barr virus (EBV) transcriptional activator that mediates a genetic switch between the latent and lytic states of the virus by binding to the promoters of genes involved in lytic DNA replication and activating their transcription. A computer survey revealed that 9 of 23 potential or known ZEBRA-resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4803-4813.1992
更新日期:1992-08-01 00:00:00
abstract::An active transcriptive complex was isolated from purified virions of Newcastle disease virus. After disruption with Triton X-100 and high salt, soluble and particulate fractions were separated by density gradient centrifugation. The transcriptive complex, recovered at a density of 1.275 g/cm3, appeared as a nucleocap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.3.1080-1089.1976
更新日期:1976-09-01 00:00:00
abstract::Q beta phage RNAs with inactivating insertion (8-base) or deletion (17-base) mutations within their replicase genes were prepared from modified Q beta cDNAs and transfected into Escherichia coli spheroplasts containing Q beta replicase provided in trans by a resident plasmid. Replicase-defective (Rep-) Q beta phage pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.4.2435-2442.1992
更新日期:1992-04-01 00:00:00
abstract::The herpes simplex virus type 1 gamma(1)34.5 gene product precludes the host-mediated protein shutoff response induced by activated protein kinase R (PKR). Earlier studies demonstrated that recombinant viruses lacking the gamma(1)34.5 gene (Deltagamma(1)34.5) developed secondary mutations that allowed earlier U(S)11 e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.5.2029-2035.2002
更新日期:2002-03-01 00:00:00
abstract:UNLABELLED:Pattern recognition receptors (PRR) sense certain molecular patterns uniquely expressed by pathogens. Retinoic-acid-inducible gene I (RIG-I) is a cytosolic PRR that senses viral nucleic acids and induces innate immune activation and secretion of type I interferons (IFNs). Here, using influenza vaccine antige...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02273-14
更新日期:2014-12-01 00:00:00
abstract::The proline-rich tandem repeat domain of human mucin MUC1 forms an extended structure containing large repeating loops that are crested by a turn. We show that the repeating-loop structure of MUC1 can be replaced by an antibody complementarity-determining region loop of a human immunodeficiency virus type 1 (HIV-1)-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6557-6562.1996
更新日期:1996-10-01 00:00:00
abstract::To determine the role of amino acid sequences of the hemagglutinin-neuraminidase (HN) cytoplasmic tail in Newcastle disease virus (NDV) replication and pathogenicity, we generated recombinant NDVs with a deletion or point mutation in the N-terminal cytoplasmic tail. The first 2-amino-acid deletion in the cytoplasmic t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01038-09
更新日期:2009-10-01 00:00:00
abstract::We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
abstract::By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.131-134.1982
更新日期:1982-04-01 00:00:00
abstract::Interferon-induced transmembrane protein 3 (IFITM3) is a cellular factor that reduces HIV-1 infectivity by an incompletely understood mechanism. We show here that viruses differing only in the envelope glycoprotein (Env) expressed on their surface have different sensitivities to IFITM3. Measurements of the sensitivity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01994-20
更新日期:2020-12-09 00:00:00
abstract::Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. In the present study, we have investigat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1764-1771.2003
更新日期:2003-02-01 00:00:00
abstract::The Kansas strain of bovine parainfluenza virus type 3 (BPIV3) is 100- to 1,000-fold restricted in replication in the respiratory tracts of nonhuman primates compared to human PIV3 (HPIV3), an important pathogen of infants and young children. BPIV3 is also restricted in replication in human infants and children, yet i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1141-1148.2003
更新日期:2003-01-01 00:00:00
abstract::Analysis of large and small plaque mutants of simian virus 40 using antisera prepared against each has revealed quantitative and possibly qualitative antigenic differences for each plaque type. A sensitive micro radioisotope precipitation test permitted evaluation of immunochemical similarities and differences of caps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.1.17-24.1969
更新日期:1969-01-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) epidemic in Southeast Asia has been largely due to the emergence of clade E (HIV-1E). It has been suggested that HIV-1E is derived from a recombinant lineage of subtype A (HIV-1A) and subtype E, with multiple breakpoints along the E genome. We obtained complete genome se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10752-10765.2000
更新日期:2000-11-01 00:00:00
abstract::Previous analyses have demonstrated that adenovirus DNA is packaged into virions in vivo in a polar, left-to-right fashion. The packaging of viral DNA is dependent on cis-acting elements at the left end of the genome. In this report, we describe a genetic analysis of the sequences that are required for efficient packa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
更新日期:1990-05-01 00:00:00