Abstract:
:Replicase gene expression by the human coronavirus 229E involves the synthesis of two large polyproteins, pp1a and pp1ab. Experimental evidence suggests that these precursor molecules are subject to extensive proteolytic processing. In this study, we show that a chymotrypsin-like enzyme, the virus-encoded 3C-like proteinase (3CLpro), cleaves within a common region of pp1a and pp1ab (amino acids 3490 to 4068) at four sites. trans-cleavage assays revealed that polypeptides of 5, 23, 12, and 16 kDa are processed from pp1a/pp1ab by proteolysis of the peptide bonds Q3546/S3547, Q3629/S3630, Q3824/N3825, and Q3933/A3934. Relative rate constants for the 3CLpro-mediated cleavages Q2965/A2966, Q3267/S3268, Q3824/N3825, and Q3933/A3934 were derived by competition experiments using synthetic peptides and recombinant 3CLpro. The results indicate that coronavirus cleavage sites differ significantly with regard to their susceptibilities to proteolysis by 3CLpro. Finally, immunoprecipitation with specific rabbit antisera was used to detect the pp1a/pp1ab processing end products in virus-infected cells, and immunofluorescence data that suggest an association of these polypeptides with intracellular membranes were obtained.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ziebuhr J,Siddell SGdoi
10.1128/JVI.73.1.177-185.1999subject
Has Abstractpub_date
1999-01-01 00:00:00pages
177-85issue
1eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::Toroviruses (family Coronaviridae, order Nidovirales) are enveloped, positive-stranded RNA viruses that have been implicated in enteric disease in cattle and possibly in humans. Despite their potential veterinary and clinical relevance, little is known about torovirus epidemiology and molecular genetics. Here, we pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9567-9577.2003
更新日期:2003-09-01 00:00:00
abstract::A total of 51 restriction sites were recognized within the BK virus genome by the combination of 10 different restriction endonucleases. These sites were mapped and oriented relative to one another as well as to the five fragments generated by the digestion of BK virus DNA with HindIII and EcoRI. The result was a comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.915-925.1979
更新日期:1979-03-01 00:00:00
abstract::The pandemic H1N1 virus of 2009 (2009 H1N1) continues to cause illness worldwide, primarily in younger age groups. To better understand the pathogenesis of these viruses in mammals, we used a mouse model to evaluate the relative virulence of selected 2009 H1N1 viruses and compared them to a representative human triple...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02742-09
更新日期:2010-05-01 00:00:00
abstract::Mus dunni endogenous virus (MDEV) is activated from cells of the Asian wild mouse M. dunni (also known as Mus terricolor) in response to treatment with either 5-iodo-2'-deoxyuridine or hydrocortisone. MDEV represents a new murine retrovirus interference group and thus appears to use a different receptor for entry into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4663-4670.1997
更新日期:1997-06-01 00:00:00
abstract::The effect of guanidine on the replication of the group A arboviruses, Sindbis virus, and Semliki Forest virus (SFV) was studied. Guanidine rapidly, but reversibly, inhibited SFV ribonucleic acid (RNA) synthesis. The synthesis of all species of viral RNA was inhibited, but that of ribonuclease-resistant forms was leas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.5.628-636.1970
更新日期:1970-11-01 00:00:00
abstract::Genetic studies with immunocompetent mice show the importance of both T cells and gamma interferon (IFN-γ) for survival of a measles virus (MV) challenge; however, the direct role of T cells and IFN-γ within the MV-infected brain has not been addressed. Organotypic brain explants represent a successful ex vivo system ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01496-10
更新日期:2011-04-01 00:00:00
abstract::We generated Chinese hamster ovary cell lines that stably express wild-type, secreted, and glycosylphosphatidylinositol (GPI)-anchored envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1). The cells expressing wild-type Env (WT cells) express both the precursor gp160 and the mature gp120/gp41 and readi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7060-7066.1993
更新日期:1993-12-01 00:00:00
abstract::We analyzed wild mouse DNAs for the number and type of proviral genes related to the env sequences of various murine leukemia viruses (MuLVs). Only Mus species closely related to laboratory mice carried these retroviral sequences, and the different subclasses of viral env genes tended to be restricted to specific taxo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3082-3088.1987
更新日期:1987-10-01 00:00:00
abstract:UNLABELLED:The ORF45 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus-specific immediate-early tegument protein. Our previous studies have revealed its crucial roles in both early and late stages of KSHV infection. In this study, we surveyed the interactome of ORF45 using a panel of monoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02925-14
更新日期:2015-05-01 00:00:00
abstract::Long interspersed nuclear element 1 (LINE-1; L1) retrotransposons are the most common retroelements in mammalian genomes. Unlike individual families of endogenous retroviruses (ERVs), they have remained active throughout the mammalian radiation and are responsible for most of the retroelement movement and much genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05180-11
更新日期:2011-12-01 00:00:00
abstract::Intracisternal-A-particle-related envelope-encoding (IAPE) proviral elements in the mouse genome encode and express an envelope-like protein that may allow transmission of IAPEs as infectious agents. To test IAPE mobility and potential transmission in mice, we have analyzed the distribution of IAPE elements in the gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.6450-6454.1996
更新日期:1996-09-01 00:00:00
abstract::A human cytomegalovirus (CMV) UL54 pol exonuclease domain II mutation, D413A, found in a clinical specimen, conferred ganciclovir (GCV) and cidofovir resistance but not foscarnet resistance when incorporated into laboratory strain T2294. After several passages without drug, mutation was observed in five of eight plaqu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-07
更新日期:2008-01-01 00:00:00
abstract::Recombinant human cytomegaloviruses that do not express UL97 kinase activity exhibit a distinctive plaque morphology characterized by the formation of highly refractile bodies late in infection. These structures were also observed in infected cells treated with the UL97 kinase inhibitor maribavir. Nuclear inclusions w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.24.15494-15502.2005
更新日期:2005-12-01 00:00:00
abstract::Rodent fibroblasts infected with the ts371 Kirsten murine sarcoma virus (KiMuSV) are temperature sensitive for the maintenance of transformation because of the production of an abnormal p21 protein. We cloned the ts371 KiMuSV provirus from the genome of a conditionally transformed nonproducer cell line, ts371 KiMuSV N...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.782-786.1986
更新日期:1986-11-01 00:00:00
abstract::We have examined the pathway of rhinovirus interaction with soluble intercellular adhesion molecule 1 (sICAM-1). Binding of sICAM-1 to rhinovirus serotypes 3 and 14 gives particles with sedimentation coefficients from 145 to 120S, depending on the amount of sICAM-1 bound. The formation of 120S particles is faster and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5882-5889.1994
更新日期:1994-09-01 00:00:00
abstract::The UL11 tegument protein of herpes simplex virus plays a critical role in the secondary envelopment; however, the mechanistic details remain elusive. Here, we report a new function of UL11 in the budding process in which it directs efficient acquisition of glycoprotein E (gE) via a direct interaction. In vitro bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05207-11
更新日期:2011-09-01 00:00:00
abstract::Viral infection triggers host innate immune responses through cellular sensor molecules which activate multiple signaling cascades that induce the production of interferons (IFN) and other cytokines. The recent identification of mammalian cytoplasmic viral RNA sensors, such as retinoic acid-inducible gene I (RIG-I)-li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00404-09
更新日期:2009-08-01 00:00:00
abstract::Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kinase R (PKR). In the case of murine cytomegalovirus (MCMV), the protein products of two essential genes, m142 and m143, bind to double-stranded RNA (dsRNA) and block phosphorylation of PKR and eukaryotic initiation factor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01484-08
更新日期:2009-02-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) R5 isolates that predominantly use CCR5 as a coreceptor are frequently described as macrophage tropic. Here, we compare macrophage tropism conferred by HIV-1 R5 envelopes that were derived directly by PCR from patient tissue. This approach avoids potentially selective cultur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02328-05
更新日期:2006-07-01 00:00:00
abstract::Two herpes simplex virus type 1 glycoproteins, gE and gI, have been shown to form a complex that binds the Fc domain of immunoglobulin G (IgG). We demonstrate that this complex is required for the binding of monomeric nonimmune IgG but that gE alone is sufficient for binding polymeric IgG in the form of IgG complexes....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2725-2731.1990
更新日期:1990-06-01 00:00:00
abstract::By using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic DNA of a novel papillomavirus was amplified from a skin lesion biopsy of a Florida manatee (Trichechus manatus latirostris), one of the most endangered marine mammals in United States coastal waters. The nucleotide seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12698-12702.2004
更新日期:2004-11-01 00:00:00
abstract::We previously described a monoclonal antibody (Y8-10C2) that binds influenza virus hemagglutinin (HA) monomers but not native trimers. In this study, we demonstrated that Y8-10C2 binds to the globular domain of HA and found evidence that its epitope is located at the interface of adjacent subunits. We further showed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.933-942.1993
更新日期:1993-02-01 00:00:00
abstract::Myeloblastosis-associated virus type 1 (MAV1) derived from a molecular clone of infectious proviral DNA (B. Perbal, J. S. Lipsick, J. Svoboda, R. F. Silva, and M. A. Baluda, J. Virol. 56:240-244, 1985) was shown to specifically induce nephroblastoma in chickens and therefore belongs to the MAV-N class. We show that ne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1803-1807.1989
更新日期:1989-04-01 00:00:00
abstract::The addition of 25 mug of protamine sulfate per ml to lysozyme-ethylenediamine-tetraacetic acid spheroplasts of Escherichia coli stimulates transfection not only for T1 phage deoxyribonucleic acid (DNA; Hotz and Mauser, 1969) but also for the following phage DNA species: lambda, 10,000-fold to an efficiency of 10(-3) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.5.646-650.1971
更新日期:1971-05-01 00:00:00
abstract::Adenovirus (Ad) gene transfer vectors can be used to transfer and express antigens and function as strong adjuvants and thus are useful platforms for the development of genetic vaccines. Based on the hypothesis that Ad vectors with enhanced infectibility of dendritic cells (DC) may be able to evoke enhanced immune res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2572-2580.2004
更新日期:2004-03-01 00:00:00
abstract::Viral infectivity factor (Vif) is required for lentivirus fitness and pathogenicity, except in equine infectious anemia virus (EIAV). Vif enhances viral infectivity by a Cullin5-Elongin B/C E3 complex to inactivate the host restriction factor APOBEC3. Core-binding factor subunit beta (CBF-β) is a cell factor that was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-14
更新日期:2014-10-01 00:00:00
abstract::Analyses, by construction of phage growth curves, indicated that the polysaccharide depolymerase was synthesized by Pseudomonas aeruginosa strains B and BI after infection with phage 2. The kinetics of biosynthesis of the depolymerase were found to parallel closely the rate of formation of phage-directed virions, and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.3.290-296.1969
更新日期:1969-03-01 00:00:00
abstract::Most patients infected with hepatitis C virus (HCV) become chronic carriers. Viruses that efficiently establish persistent infections must have effective ways of evading host defenses. In the case of HCV, little is known about how chronic infections are established or maintained. Besides hepatocytes, several reports s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.772-781.2001
更新日期:2001-01-01 00:00:00
abstract::The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.8151-8154.1995
更新日期:1995-12-01 00:00:00
abstract::The intracellular assembly site for flaviviruses in currently not known but is presumed to be located within the lumen of the rough endoplasmic reticulum (RER). Building on previous studies involving immunofluorescence (IF) and cryoimmunoelectron microscopy of Kunjin virus (KUN)-infected cells, we sought to identify t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.10787-10799.2001
更新日期:2001-11-01 00:00:00