Abstract:
:Human immunodeficiency virus type 1 (HIV-1) R5 isolates that predominantly use CCR5 as a coreceptor are frequently described as macrophage tropic. Here, we compare macrophage tropism conferred by HIV-1 R5 envelopes that were derived directly by PCR from patient tissue. This approach avoids potentially selective culture protocols used in virus isolation. Envelopes were amplified (i) from blood and semen of adult patients and (ii) from plasma of pediatric patients. The phenotypes of these envelopes were compared to those conferred by an extended panel of envelopes derived from brain and lymph node that we reported previously. Our results show that R5 envelopes vary by up to 1,000-fold in their capacity to confer infection of primary macrophages. Highly macrophage-tropic envelopes were predominate in brain but were infrequent in semen, blood, and lymph node samples. We also confirmed that the presence of N283 in the C2 CD4 binding site of gp120 is associated with HIV-1 envelopes from the brain but absent from macrophage-tropic envelopes amplified from blood and semen. Finally, we compared infection of macrophages, CD4(+) T cells, and peripheral blood mononuclear cells (PBMCs) conferred by macrophage-tropic and non-macrophage-tropic envelopes in the context of full-length replication competent viral clones. Non-macrophage-tropic envelopes conferred low-level infection of macrophages yet infected CD4(+) T cells and PBMCs as efficiently as highly macrophage-tropic brain envelopes. The lack of macrophage tropism for the majority of the envelopes amplified from lymph node, blood, and semen is striking and contrasts with the current consensus that R5 primary isolates are generally macrophage tropic. The extensive variation in R5 tropism reported here is likely to have an important impact on pathogenesis and on the capacity of HIV-1 to transmit.
journal_name
J Viroljournal_title
Journal of virologyauthors
Peters PJ,Sullivan WM,Duenas-Decamp MJ,Bhattacharya J,Ankghuambom C,Brown R,Luzuriaga K,Bell J,Simmonds P,Ball J,Clapham PRdoi
10.1128/JVI.02328-05subject
Has Abstractpub_date
2006-07-01 00:00:00pages
6324-32issue
13eissn
0022-538Xissn
1098-5514pii
80/13/6324journal_volume
80pub_type
杂志文章abstract::Adenovirus infections cause significant morbidity and mortality in immunocompromised patients, yet little is known about the immune response to adenovirus infections. We established a system for the generation of a cytotoxic immune response to adenovirus in vitro. Cytotoxic T cells (CTLs) were derived from normal dono...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6733-6740.1996
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更新日期:1976-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.25.3.923-927.1978
更新日期:1978-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1979-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5449-5456.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.1.137-144.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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更新日期:2015-01-15 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.198-205.1995
更新日期:1995-01-01 00:00:00