Abstract:
:Our study was designed to investigate the nature of an antigen-specific suppressor factor generated by antigen-stimulated herpes simplex virus (HSV)-immune splenocytes. Factor SF-200, a 90,000- to 100,000-dalton fraction obtained after Sephacryl gel filtration, suppressed the generation of HSV-specific cytotoxic T-lymphocyte and lymphoproliferative responses. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of SF-200 indicated that it contained an I-J+, anti-idiotypic protein. It was possible to adsorb the suppressor activity of SF-200 to an anti-I-J immunoaffinity column. The suppressor activity could be eluted from the immunoaffinity column with a low-pH buffer. The acid-eluted material was determined to be both I-J+ and reactive with anti-HSV antiserum by Western blot analysis. Both SF-200 and the I-J+ suppressor activity suppressed only HSV-specific cell-mediated immunity responses. However, it was possible to generate nonspecific suppressor activity by incubating the I-J+ suppressor factor with Lyt 1+ splenocytes from HSV-immune mice. The implication of these results with respect to the model for a suppressor cell circuit regulating HSV-specific cell-mediated immunity responses is discussed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Horohov DW,Wyckoff JH 3rd,Moore RN,Rouse BTdoi
10.1128/JVI.58.2.331-338.1986subject
Has Abstractpub_date
1986-05-01 00:00:00pages
331-8issue
2eissn
0022-538Xissn
1098-5514journal_volume
58pub_type
杂志文章abstract::Upon infection of Japanese encephalitis virus (JEV), baby hamster kidney (BHK-21) and Chinese hamster ovary (CHO) cells were killed by a mechanism involved in apoptosis. While readily established in a variety of cell lines, JEV persistence has never been successfully instituted in BHK-21 and CHO cells. Since stable ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.9844-9854.1998
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.131-134.1982
更新日期:1982-04-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.71.7.5688-5691.1997
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abstract:UNLABELLED:Next-generation sequence analysis of virus-like particles (VLPs) produced during agroinfiltration of cucumber necrosis virus (CNV) coat protein (CP) and of authentic CNV virions was conducted to assess if host RNAs can be encapsidated by CNV CP. VLPs containing host RNAs were found to be produced during agro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01466-15
更新日期:2015-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.111-122.1994
更新日期:1994-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00342-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8446-8452.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2825-2831.1999
更新日期:1999-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.931-936.1973
更新日期:1973-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1782-1789.1994
更新日期:1994-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02030-10
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03459-12
更新日期:2013-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.2.323-337.1977
更新日期:1977-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5224-5231.1992
更新日期:1992-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1837-1849.2006
更新日期:2006-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.5978-5983.1998
更新日期:1998-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03433-12
更新日期:2013-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3451-3454.1998
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pub_type: 杂志文章
doi:10.1128/JVI.67.12.7612-7617.1993
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pub_type: 杂志文章
doi:10.1128/JVI.19.3.810-819.1976
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.772-785.1978
更新日期:1978-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.9.3708-3713.1989
更新日期:1989-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12674-12680.2005
更新日期:2005-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1181-1189.1995
更新日期:1995-02-01 00:00:00
abstract::During paramyxovirus entry into a host cell, receptor engagement by a specialized binding protein triggers conformational changes in the adjacent fusion protein (F), leading to fusion between the viral and cell membranes. According to the existing paradigm of paramyxovirus membrane fusion, the initial activation of F ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05873-11
更新日期:2011-12-01 00:00:00