HIV-1-specific interleukin-21+ CD4+ T cell responses contribute to durable viral control through the modulation of HIV-specific CD8+ T cell function.

Abstract:

:Functional defects in cytotoxic CD8(+) T cell responses arise in chronic human viral infections, but the mechanisms involved are not well understood. In mice, CD4 cell-mediated interleukin-21 (IL-21) production is necessary for the maintenance of CD8(+) T cell function and control of persistent viral infections. To investigate the potential role of IL-21 in a chronic human viral infection, we studied the rare subset of HIV-1 controllers, who are able to spontaneously control HIV-1 replication without treatment. HIV-specific triggering of IL-21 by CD4(+) T cells was significantly enriched in these persons (P = 0.0007), while isolated loss of IL-21-secreting CD4(+) T cells was characteristic for subjects with persistent viremia and progressive disease. IL-21 responses were mediated by recognition of discrete epitopes largely in the Gag protein, and expansion of IL-21(+) CD4(+) T cells in acute infection resulted in lower viral set points (P = 0.002). Moreover, IL-21 production by CD4(+) T cells of HIV controllers enhanced perforin production by HIV-1-specific CD8(+) T cells from chronic progressors even in late stages of disease, and HIV-1-specific effector CD8(+) T cells showed an enhanced ability to efficiently inhibit viral replication in vitro after IL-21 binding. These data suggest that HIV-1-specific IL-21(+) CD4(+) T cell responses might contribute to the control of viral replication in humans and are likely to be of great importance for vaccine design.

journal_name

J Virol

journal_title

Journal of virology

authors

Chevalier MF,Jülg B,Pyo A,Flanders M,Ranasinghe S,Soghoian DZ,Kwon DS,Rychert J,Lian J,Muller MI,Cutler S,McAndrew E,Jessen H,Pereyra F,Rosenberg ES,Altfeld M,Walker BD,Streeck H

doi

10.1128/JVI.02030-10

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

733-41

issue

2

eissn

0022-538X

issn

1098-5514

pii

JVI.02030-10

journal_volume

85

pub_type

杂志文章
  • Mutations in the membrane-proximal region of the influenza A virus M2 protein cytoplasmic tail have modest effects on virus replication.

    abstract::Influenza A virus encodes M2, a proton channel that has been shown to be important during virus entry and assembly. In order to systematically investigate the role of the membrane-proximal residues in the M2 cytoplasmic tail in virus replication, we utilized scanning and directed alanine mutagenesis in combination wit...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.05970-11

    authors: Stewart SM,Pekosz A

    更新日期:2011-12-01 00:00:00

  • Biological activities of a synthetic peptide composed of two unlinked domains from a retroviral transmembrane protein sequence.

    abstract::We report several biological activities of a synthetic peptide whose sequence contains the highly conserved region of feline leukemia virus transmembrane protein (TM) synthetically linked to another short TM-derived sequence particularly rich in polar positive residues. This 29-amino-acid peptide blocked [3H]thymidine...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.4.1429-1436.1990

    authors: Wegemer DE,Kabat KG,Kloetzer WS

    更新日期:1990-04-01 00:00:00

  • Polysomal localization of R17 bacteriophage-specific protein synthesis.

    abstract::Synthesis of viral ribonucleic acid (RNA) polymerase, maturation protein, and coat protein in Escherichia coli infected with bacteriophage R17 occurs mainly on polysomes containing four or more ribosomes. The 30S ribosomal subunits through trimer-size polysomes, which are associated with all of the R17-specific protei...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.9.1.75-84.1972

    authors: Truden JL,Franklin RM

    更新日期:1972-01-01 00:00:00

  • Effect of water-based microencapsulation on protection against EDIM rotavirus challenge in mice.

    abstract::We determined the capacity of microcapsules formed by the combination of sodium alginate, an aqueous anionic polymer, and spermine hydrochloride, an aqueous cationic amine, to enhance protection against rotavirus challenge in mice. Adult BALB/c mice were orally inoculated with either free or microencapsulated rotaviru...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.5.3859-3862.1998

    authors: Moser CA,Speaker TJ,Offit PA

    更新日期:1998-05-01 00:00:00

  • Potent, broad-spectrum inhibition of human immunodeficiency virus type 1 by the CCR5 monoclonal antibody PRO 140.

    abstract::CCR5 serves as a requisite fusion coreceptor for clinically relevant strains of human immunodeficiency virus type 1 (HIV-1) and provides a promising target for antiviral therapy. However, no study to date has examined whether monoclonal antibodies, small molecules, or other nonchemokine agents possess broad-spectrum a...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.2.579-588.2001

    authors: Trkola A,Ketas TJ,Nagashima KA,Zhao L,Cilliers T,Morris L,Moore JP,Maddon PJ,Olson WC

    更新日期:2001-01-01 00:00:00

  • Ebola virus VP24 proteins inhibit the interaction of NPI-1 subfamily karyopherin alpha proteins with activated STAT1.

    abstract::The Zaire ebolavirus protein VP24 was previously demonstrated to inhibit alpha/beta interferon (IFN-alpha/beta)- and IFN-gamma-induced nuclear accumulation of tyrosine-phosphorylated STAT1 (PY-STAT1) and to inhibit IFN-alpha/beta- and IFN-gamma-induced gene expression. These properties correlated with the ability of V...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01097-07

    authors: Reid SP,Valmas C,Martinez O,Sanchez FM,Basler CF

    更新日期:2007-12-01 00:00:00

  • Regulation of VP30-Dependent Transcription by RNA Sequence and Structure in the Genomic Ebola Virus Promoter.

    abstract::Viral transcription and replication of Ebola virus (EBOV) is balanced by transcription factor VP30, an RNA binding protein. An RNA hairpin at the transcription start site (TSS) of the first gene (NP hairpin) in the 3'-leader promoter is thought to mediate the VP30 dependency of transcription. Here, we investigated the...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02215-20

    authors: Bach S,Demper JC,Grünweller A,Becker S,Biedenkopf N,Hartmann RK

    更新日期:2020-12-02 00:00:00

  • Transcription of DNA from the 70S RNA of Rous sarcoma virus. II. Structure of a 4S RNA primer.

    abstract::The 70S RNA of Rous sarcoma virus contains 4S RNAs which serve as primers for the initiation of DNA synthesis in vitro by the RNA-directed DNA polymerase of the virus. We purified these primers in three different ways-by isolation of the covalent complex between primer and nascent DNA, by differential melting of the 7...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.13.5.1134-1142.1974

    authors: Faras AJ,Dahlberg JE,Sawyer RC,Harada F,Taylor JM,Levinson WE,Bishop JM,Goodman HM

    更新日期:1974-05-01 00:00:00

  • Transcription of the derepressed open reading frame P of herpes simplex virus 1 precludes the expression of the antisense gamma(1)34.5 gene and may account for the attenuation of the mutant virus.

    abstract::Open reading frame P (ORF P), located at the 3' terminus of the 8.5-kb DNA sequence transcribed during latency and almost completely antisense to the gamma(1)34.5 gene, is naturally repressed by infected cell protein 4 (ICP4), the major herpes simplex virus 1 regulatory protein. Earlier studies on cells infected with ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.71.10.7750-7757.1997

    authors: Randall G,Roizman B

    更新日期:1997-10-01 00:00:00

  • Polyomavirus large T antigen binds the transcriptional coactivator protein p300.

    abstract::Using coimmunoprecipitation and glutathione S-transferase pulldown experiments, we found that polyomavirus large T antigen binds to p300 in vivo and in vitro. The N-terminal region of the viral protein, including the pRB binding motif, was dispensable for this interaction, which involved several regions within the C-t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.2.1734-1739.1999

    authors: Nemethova M,Wintersberger E

    更新日期:1999-02-01 00:00:00

  • Effect of calcium-modulating cyclophilin ligand on human immunodeficiency virus type 1 particle release and cell surface expression of tetherin.

    abstract::The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpu enhances virus particle release by counteracting a host factor that retains virions at the surfaces of infected cells. It was recently demonstrated that cellular protein BST-2/CD317/Tetherin restricts HIV-1 release in a Vpu-dependent manner. Calcium...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01786-09

    authors: Bego MG,Dubé M,Mercier J,Cohen EA

    更新日期:2009-12-01 00:00:00

  • Varicella-zoster viral glycoproteins analyzed with monoclonal antibodies.

    abstract::Monoclonal antibodies to varicella-zoster virus were used to study viral glycoproteins by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Based on the viral glycoproteins immunoprecipitated, the five monoclonal antibodies fell into three groups. Two antibodies, 4B7 and 8G9 (group 1),...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.52.1.55-62.1984

    authors: Forghani B,Dupuis KW,Schmidt NJ

    更新日期:1984-10-01 00:00:00

  • Alpha interferon induces distinct translational control programs to suppress hepatitis C virus RNA replication.

    abstract::Hepatitis C virus (HCV) infection is treated with interferon (IFN)-based therapy. The mechanisms by which IFN suppresses HCV replication are not known, and only limited efficacy is achieved with therapy because the virus directs mechanisms to resist the host IFN response. In the present study we characterized the effe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.7.3898-3912.2003

    authors: Wang C,Pflugheber J,Sumpter R Jr,Sodora DL,Hui D,Sen GC,Gale M Jr

    更新日期:2003-04-01 00:00:00

  • Characterization of Marek's disease virus serotype 1 (MDV-1) deletion mutants that lack UL46 to UL49 genes: MDV-1 UL49, encoding VP22, is indispensable for virus growth.

    abstract::Experiments were conducted to investigate the roles of Marek's disease virus serotype 1 (MDV-1) major tegument proteins VP11/12, VP13/14, VP16, and VP22 in viral growth in cultured cells. Based on a bacterial artificial chromosome clone of MDV-1 (BAC20), mutant viruses were constructed in which the MDV-1 homologs of U...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.4.1959-1970.2002

    authors: Dorange F,Tischer BK,Vautherot JF,Osterrieder N

    更新日期:2002-02-01 00:00:00

  • Dengue virus-specific human CD4+ T-lymphocyte responses in a recipient of an experimental live-attenuated dengue virus type 1 vaccine: bulk culture proliferation, clonal analysis, and precursor frequency determination.

    abstract::We analyzed the CD4+ T-lymphocyte responses to dengue, West Nile, and yellow fever viruses 4 months after immunization of a volunteer with an experimental live-attenuated dengue virus type 1 vaccine (DEN-1 45AZ5). We examined bulk culture proliferation to noninfectious antigens, determined the precursor frequency of s...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.67.10.5962-5967.1993

    authors: Green S,Kurane I,Edelman R,Tacket CO,Eckels KH,Vaughn DW,Hoke CH Jr,Ennis FA

    更新日期:1993-10-01 00:00:00

  • Detection of novel sequences related to african Swine Fever virus in human serum and sewage.

    abstract::The family Asfarviridae contains only a single virus species, African swine fever virus (ASFV). ASFV is a viral agent with significant economic impact due to its devastating effects on populations of domesticated pigs during outbreaks but has not been reported to infect humans. We report here the discovery of novel vi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00638-09

    authors: Loh J,Zhao G,Presti RM,Holtz LR,Finkbeiner SR,Droit L,Villasana Z,Todd C,Pipas JM,Calgua B,Girones R,Wang D,Virgin HW

    更新日期:2009-12-01 00:00:00

  • Inflammatory monocytes recruited to the liver within 24 hours after virus-induced inflammation resemble Kupffer cells but are functionally distinct.

    abstract:UNLABELLED:Due to a scarcity of immunocompetent animal models for viral hepatitis, little is known about the early innate immune responses in the liver. In various hepatotoxic models, both pro- and anti-inflammatory activities of recruited monocytes have been described. In this study, we compared the effect of liver in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03733-14

    authors: Movita D,van de Garde MD,Biesta P,Kreefft K,Haagmans B,Zuniga E,Herschke F,De Jonghe S,Janssen HL,Gama L,Boonstra A,Vanwolleghem T

    更新日期:2015-05-01 00:00:00

  • Within-Host Variations of Human Papillomavirus Reveal APOBEC Signature Mutagenesis in the Viral Genome.

    abstract::Persistent infection with oncogenic human papillomaviruses (HPVs) causes cervical cancer, accompanied by the accumulation of somatic mutations into the host genome. There are concomitant genetic changes in the HPV genome during viral infection; however, their relevance to cervical carcinogenesis is poorly understood. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00017-18

    authors: Hirose Y,Onuki M,Tenjimbayashi Y,Mori S,Ishii Y,Takeuchi T,Tasaka N,Satoh T,Morisada T,Iwata T,Miyamoto S,Matsumoto K,Sekizawa A,Kukimoto I

    更新日期:2018-05-29 00:00:00

  • Disruption of the murine gammaherpesvirus 68 M1 open reading frame leads to enhanced reactivation from latency.

    abstract::Murine gammaherpesvirus 68 (gammaHV68, or MHV-68) is a genetically tractable, small animal model for the analysis of gammaherpesvirus pathogenesis. The gammaHV68 genome is colinear with the genomes of other sequence gammaherpesviruses, containing large blocks of conserved genes interspersed by a number of putative gen...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.4.1973-1984.2000

    authors: Clambey ET,Virgin HW 4th,Speck SH

    更新日期:2000-02-01 00:00:00

  • Structure and leukemogenic activity of a murine leukemia virus.

    abstract::Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, o...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.2.4.365-375.1968

    authors: De Tkaczevski L,De Harven E,Friend C

    更新日期:1968-04-01 00:00:00

  • Functional analysis of the complex trans-activating response element RNA structure in simian immunodeficiency virus.

    abstract::Transcription of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) is activated through binding of the viral Tat protein to the trans-activating response (TAR) element at the 5' end of the nascent transcript. Whereas HIV type 1 (HIV-1) TAR folds a simple hairpin structure, the corresponding do...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00530-08

    authors: Centlivre M,Klaver B,Berkhout B,Das AT

    更新日期:2008-09-01 00:00:00

  • Development of an Alternative Modified Live Influenza B Virus Vaccine.

    abstract::Influenza B virus (IBV) is considered a major human pathogen, responsible for seasonal epidemics of acute respiratory illness. Two antigenically distinct IBV hemagglutinin (HA) lineages cocirculate worldwide with little cross-reactivity. Live attenuated influenza virus (LAIV) vaccines have been shown to provide better...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00056-17

    authors: Santos JJS,Finch C,Sutton T,Obadan A,Aguirre I,Wan Z,Lopez D,Geiger G,Gonzalez-Reiche AS,Ferreri L,Perez DR

    更新日期:2017-05-26 00:00:00

  • DNA polymerase activities in growing cells infected with simian virus 40.

    abstract::Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity o...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.23.1.117-125.1977

    authors: Méchali M,Girard M,de Recondo AM

    更新日期:1977-07-01 00:00:00

  • Inhibition of human immunodeficiency virus type 1 production in infected peripheral blood mononuclear cells by human leukocyte antigen class I-specific antibodies: evidence for a novel antiviral mechanism.

    abstract::A well-characterized mechanism by which anti-HLA class I monoclonal antibodies (MAb) inhibit human immunodeficiency virus type 1 (HIV-1) propagation in in vitro cell cultures is the neutralization of the virus through interactions with HLA molecules associated with the virion envelope. Yet, the possibility that anothe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.8.5213-5220.1996

    authors: Briant L,Benkirane M,Girard M,Hirn M,Iosef C,Devaux C

    更新日期:1996-08-01 00:00:00

  • Differences in cytokine and chemokine responses during neurological disease induced by polytropic murine retroviruses Map to separate regions of the viral envelope gene.

    abstract::Infection of the central nervous system (CNS) by several viruses can lead to upregulation of proinflammatory cytokines and chemokines. In immunocompetent adults, these molecules induce prominent inflammatory infiltrates. However, with immunosuppressive retroviruses, such as human immunodeficiency virus (HIV), little C...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.75.6.2848-2856.2001

    authors: Peterson KE,Robertson SJ,Portis JL,Chesebro B

    更新日期:2001-03-01 00:00:00

  • Treatment with anti-LFA-1 delays the CD8+ cytotoxic-T-lymphocyte response and viral clearance in mice with primary respiratory syncytial virus infection.

    abstract::Cytotoxic T lymphocytes (CTLs) play an important role in the immune response against respiratory syncytial virus (RSV) infection. The cell surface molecule lymphocyte function-associated antigen 1 (LFA-1) is an important contributor to CTL activation, CTL-mediated direct cell lysis, and lymphocyte migration. In an att...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.78.6.3014-3023.2004

    authors: Rutigliano JA,Johnson TR,Hollinger TN,Fischer JE,Aung S,Graham BS

    更新日期:2004-03-01 00:00:00

  • Nuclear localization of the duck hepatitis B virus capsid protein: detection and functional implications of distinct subnuclear bodies in a compartment associated with RNA synthesis and maturation.

    abstract::We have reinvestigated the subcellular distribution of the duck hepatitis B virus (DHBV) core protein in infected duck hepatocytes and in transfected cells. By using indirect immunofluorescence, the protein was found to be localized not only in the cytoplasm, as described previously, but also within the cell nucleus, ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.3.2157-2164.2003

    authors: Mabit H,Knaust A,Breiner KM,Schaller H

    更新日期:2003-02-01 00:00:00

  • Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses.

    abstract::This paper reports the use of zonal ultracentrifuge techniques to conduct biophysical studies of rhinoviruses grown with WI-38 cells. Good clean-out of infectivity from rhinovirus harvests was obtained with the continuous-flow B-V and B-IX rotors. Use of the B-V rotor resulted in the successful concentration of rhinov...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.2.9.937-943.1968

    authors: Gerin JL,Richter WR,Fenters JD,Holper JC

    更新日期:1968-09-01 00:00:00

  • Mapping Murine Corneal Neovascularization and Weight Loss Virulence Determinants in the Herpes Simplex Virus 1 Genome and the Detection of an Epistatic Interaction between the UL and IRS/US Regions.

    abstract:UNLABELLED:Herpes simplex virus 1 (HSV-1) most commonly causes recrudescent labial ulcers; however, it is also the leading cause of infectious blindness in developed countries. Previous research in animal models has demonstrated that the severity of HSV-1 ocular disease is influenced by three main factors: host innate ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00821-16

    authors: Lee K,Kolb AW,Larsen I,Craven M,Brandt CR

    更新日期:2016-08-26 00:00:00

  • Dysregulation through the NF-kappaB enhancer and TATA box of the human immunodeficiency virus type 1 subtype E promoter.

    abstract::The global diversity of human immunodeficiency virus type 1 (HIV-1) genotypes, termed subtypes A to J, is considerable and growing. However, relatively few studies have provided evidence for an associated phenotypic divergence. Recently, we demonstrated subtype-specific functional differences within the long terminal ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.10.8446-8452.1998

    authors: Montano MA,Nixon CP,Essex M

    更新日期:1998-10-01 00:00:00