Molecular dissection of influenza virus nucleoprotein: deletion mapping of the RNA binding domain.

abstract：:The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...

journal_title：Journal of virology

authors： Weaver EA,Lu Z,Camacho ZT,Moukdar F,Liao HX,Ma BJ,Muldoon M,Theiler J,Nabel GJ,Letvin NL,Korber BT,Hahn BH,Haynes BF,Gao F

更新日期：2006-07-01 00:00:00

abstract：UNLABELLED:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retrov...

journal_title：Journal of virology

authors： Whisnant AW,Kehl T,Bao Q,Materniak M,Kuzmak J,Löchelt M,Cullen BR

更新日期：2014-05-01 00:00:00

abstract：:Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...

journal_title：Journal of virology

authors： Bishop DH,Repik P,Obijeski JF,Moore NF,Wagner RR

更新日期：1975-07-01 00:00:00

abstract：:After isolation, the DNA of simian virus 40 appeared as a negative supertwist (form I) or as an open circle with at least one single-strand scission (form II). Under the denaturation conditions usually applied, such as heating in the presence of formaldehyde or application of alkali, form I molecules could appear as "...

journal_title：Journal of virology

authors： Mayer F,Mazaitis AJ,Pühler A

更新日期：1975-03-01 00:00:00

abstract：:Translation of picornavirus RNAs is mediated by internal ribosomal entry site (IRES) elements and requires both standard eukaryotic translation initiation factors (eIFs) and IRES-specific cellular trans-acting factors (ITAFs). Unr, a cytoplasmic RNA-binding protein that contains five cold-shock domains and is encoded ...

journal_title：Journal of virology

authors： Boussadia O,Niepmann M,Créancier L,Prats AC,Dautry F,Jacquemin-Sablon H

更新日期：2003-03-01 00:00:00

abstract：:DDX21 regulates the biogenesis of rRNA and transcription of ribonucleoprotein genes. Recently, it has been reported that DDX21 regulates the growth of some RNA viruses through various mechanisms, such as inhibiting viral genome replication, suppressing virion assembly and release, and modulating antiviral immune respo...

journal_title：Journal of virology

authors： Hao H,Han T,Xuan B,Sun Y,Tang S,Yue N,Qian Z

更新日期：2019-11-26 00:00:00

abstract：:The wild-type strain of vesicular stomatitis virus (VSV) contains in its complete virion (VSV-1, B particles) a minus strand RNA. The principle defective particle of the wild-type strain (VSV-111, T particles) contains a shorter minus strand, homologous to part of the VSV-1 genome. Neither virion contains any detectab...

journal_title：Journal of virology

authors： Roy P,Repik P,Hefti E,Bishop DH

更新日期：1973-06-01 00:00:00

abstract：:Like other herpesviruses, Kaposi's sarcoma-associated herpesvirus (KSHV, also designated human herpesvirus 8) can establish a latent infection in the infected host. During latency a small number of genes are expressed. One of those genes encodes latency-associated nuclear antigen (LANA), which is constitutively expres...

journal_title：Journal of virology

authors： Lan K,Kuppers DA,Verma SC,Robertson ES

更新日期：2004-06-01 00:00:00

abstract：:Herpesviruses are capable of several types of infection of a host cell. To investigate the early events which ultimately determine the nature of the virus-host cell interaction, a system was established utilizing temperature-sensitive mutants of herpes simplex virus type 2. Four mutants have been isolated which fail t...

journal_title：Journal of virology

authors： Koment RW,Rapp F

更新日期：1975-04-01 00:00:00

abstract：:The replication proteins Rep and Rep' of porcine circovirus type 1 (PCV1) are both capable of introducing and resealing strand discontinuities at the viral origin of DNA replication in vitro underlying genome amplification by rolling-circle replication. The PCV1 origin of replication encompasses the minimal binding si...

journal_title：Journal of virology

authors： Steinfeldt T,Finsterbusch T,Mankertz A

更新日期：2007-06-01 00:00:00

abstract：:We investigated the features of the Moloney murine sarcoma virus leader sequence necessary for RNA packaging function by using a deletion analysis approach. We found that sequences that extend beyond those characterized genetically in previous reports are important for optimal packaging efficiency. A fragment covering...

journal_title：Journal of virology

authors： Izmailova E,Aldovini A

更新日期：2002-05-01 00:00:00

abstract：:Nucleocapsid assembly in hepadnavirus replication requires selective encapsidation of the pregenomic RNA template and the viral polymerase by the core proteins. It has been shown that an encapsidation signal located at the 5' end of the pregenomic RNA is responsible for its interaction with the polymerase. In the pres...

journal_title：Journal of virology

authors： Howe AY,Tyrrell DL

更新日期：1996-08-01 00:00:00

abstract：:Lentivectors stimulate potent immune responses to antigen transgenes and are being developed as novel genetic vaccines. To improve safety while retaining efficacy, we constructed a lentivector in which transgene expression was restricted to antigen-presenting cells using the mouse dectin-2 gene promoter. This lentivec...

journal_title：Journal of virology

authors： Lopes L,Dewannieux M,Gileadi U,Bailey R,Ikeda Y,Whittaker C,Collin MP,Cerundolo V,Tomihari M,Ariizumi K,Collins MK

更新日期：2008-01-01 00:00:00

abstract：:Purified nuclei from polyoma-infected mouse (3T3) cells were found to be greatly reduced in their ability to synthesize viral DNA in vitro when compared with a crude system consisting of an unfractionated hypotonic lysate of the infected cells. The synthetic capacity of the nuclei could be fully reconstituted when a h...

journal_title：Journal of virology

authors： Francke B,Hunter T

更新日期：1975-01-01 00:00:00

abstract：:The combination of an amino acid deletion at codon 67 (delta 67) and Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is associated with high-level resistance to multiple RT inhibitors. To determine the relative contributions of the delta 67 and T69G...

journal_title：Journal of virology

authors： Imamichi T,Berg SC,Imamichi H,Lopez JC,Metcalf JA,Falloon J,Lane HC

更新日期：2000-12-01 00:00:00

abstract：:The NS3 protein of hepatitis C virus (HCV) possesses protease activity responsible for the proteolytic cleavage of the viral polyprotein at the junctions of nonstructural proteins downstream of NS3. The NS3 protein was also found to be internally cleaved. In this study, we demonstrated that internal cleavages occurred...

journal_title：Journal of virology

authors： Kou YH,Chang MF,Wang YM,Hung TM,Chang SC

更新日期：2007-08-01 00:00:00

abstract：:The genome of infectious bursal disease virus consists of two segments of double-stranded RNA of 2.5 X 10(6) and 2.2 X 10(6) molecular weight. Polyacrylamide gel electrophoresis of purified virus resolved four structural polypeptides: VP-1 (90,000), VP-2 (41,000), VP-3 (35,000), and VP-4 (28,000). Peptide map comparis...

journal_title：Journal of virology

authors： Dobos P

更新日期：1979-12-01 00:00:00

abstract：:Using synthetic peptides, we characterized the B-lymphocyte (antibody) and T-lymphocyte (proliferation) responses to an immunodominant epitope of human immunodeficiency virus type 1 (HIV-1) located near the amino-terminal end of the transmembrane glycoprotein (env amino acids 598 to 609). Both immunoglobulin M (IgM) a...

journal_title：Journal of virology

authors： Schrier RD,Gnann JW Jr,Langlois AJ,Shriver K,Nelson JA,Oldstone MB

更新日期：1988-08-01 00:00:00

abstract：:Human T-cell leukemia virus type 1 (HTLV-1) Tax protein activates viral transcription from the long terminal repeats (LTR). Mechanisms through which Tax activates LTR have been established, but coactivators of this process remain to be identified and characterized. Here we show that all three members of the TORC famil...

journal_title：Journal of virology

authors： Siu YT,Chin KT,Siu KL,Yee Wai Choy E,Jeang KT,Jin DY

更新日期：2006-07-01 00:00:00

abstract：:Integration of viral DNA into the host chromosome is an essential step in the life cycle of retroviruses and is facilitated by the viral integrase enzyme. The first generation of integrase inhibitors recently approved or currently in late-stage clinical trials shows great promise for the treatment of human immunodefic...

journal_title：Journal of virology

authors： Goethals O,Clayton R,Van Ginderen M,Vereycken I,Wagemans E,Geluykens P,Dockx K,Strijbos R,Smits V,Vos A,Meersseman G,Jochmans D,Vermeire K,Schols D,Hallenberger S,Hertogs K

更新日期：2008-11-01 00:00:00

abstract：:Intracellular mature vaccinia virions are wrapped by cisternae, derived from virus-modified trans-Golgi or endosomal membranes, and then transported via microtubules to the cell periphery. Two viral proteins, encoded by the F13L and B5R open reading frames, are essential for the membrane-wrapping step. Previous transf...

journal_title：Journal of virology

authors： Husain M,Moss B

更新日期：2002-08-01 00:00:00

abstract：:Human herpesvirus 8 (HHV8) open reading frame (ORF) 21 is predicted to encode a protein similar to the thymidine kinase (TK) enzyme of other herpesviruses. Expressed in mammalian cells, ORF 21 was found to have low TK activity, based on poor growth in media containing hypoxanthine-aminopterin-thymidine (HAT) and low i...

journal_title：Journal of virology

authors： Gustafson EA,Schinazi RF,Fingeroth JD

更新日期：2000-01-01 00:00:00

abstract：:Six monoclonal antibodies were isolated that exhibited specificity for a furin cleavage site deletion mutant (V3526) of Venezuelan equine encephalitis virus (VEEV). These antibodies comprise a single competition group and bound the E3 glycoprotein of VEEV subtype I viruses but failed to bind the E3 glycoprotein of oth...

journal_title：Journal of virology

authors： Parker MD,Buckley MJ,Melanson VR,Glass PJ,Norwood D,Hart MK

更新日期：2010-12-01 00:00:00

abstract：:The Tf1 retrotransposon of Schizosaccharomyces pombe represents a group of eukaryotic long terminal repeat (LTR) retroelements that, based on their sequences, were predicted to use an RNA self-primer for initiating reverse transcription while synthesizing the negative-sense DNA strand. This feature is substantially di...

journal_title：Journal of virology

authors： Hizi A

更新日期：2008-11-01 00:00:00

abstract：:The N-terminal alpha-helix domain of the human immunodeficiency virus type 1 (HIV-1) Nef protein plays important roles in enhancement of viral infectivity, virion incorporation of Nef, and the down-regulation of major histocompatibility complex class I (MHC-I) expression on cell surfaces. In this study, we demonstrate...

journal_title：Journal of virology

authors： Akari H,Arold S,Fukumori T,Okazaki T,Strebel K,Adachi A

更新日期：2000-03-01 00:00:00

abstract：:Avian leukosis virus subgroup J (ALV-J), an exogenous avian retrovirus, is thought to have evolved by recombination with the highly identical env gene of the endogenous avian retrovirus EAV-HP. Embryonic expression of EAV-HP env has been suggested to be associated with the induction of immunological tolerance, a featu...

journal_title：Journal of virology

authors： Sacco MA,Venugopal K

更新日期：2001-12-01 00:00:00

abstract：:NF-kappaB signaling is critical to the survival and transformation of cells infected by the human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. Here we have examined how elimination of the NF-kappaB transcription factor p50 from mice affects the life cycle of murine gammaherpesviru...

journal_title：Journal of virology

authors： Krug LT,Collins CM,Gargano LM,Speck SH

更新日期：2009-05-01 00:00:00

abstract：:Galectin-9 is a pleiotropic immune modulator affecting numerous cell types of innate and adaptive immunity. Patients with chronic infection with either hepatitis C virus (HCV) or HIV have elevated circulating levels. Limited data exist on the regulation of natural killer (NK) cell function through interaction with gal...

journal_title：Journal of virology

authors： Golden-Mason L,McMahan RH,Strong M,Reisdorph R,Mahaffey S,Palmer BE,Cheng L,Kulesza C,Hirashima M,Niki T,Rosen HR

更新日期：2013-05-01 00:00:00

abstract：:Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the b...

journal_title：Journal of virology

authors： Bohl J,Hull B,Vande Pol SB

更新日期：2001-01-01 00:00:00

abstract：:The initial host response to viral infection occurs after Toll-like receptors (TLRs) on dendritic cells (DC) are stimulated by viral nucleic acids (double-stranded RNA, single-stranded RNA) and alpha interferon (IFN-alpha) and IFN-beta are produced. We hypothesized that pharmacologic induction of innate antiviral resp...

journal_title：Journal of virology

authors： Wang Y,Abel K,Lantz K,Krieg AM,McChesney MB,Miller CJ

更新日期：2005-11-01 00:00:00