Abstract:
:Nucleocapsid assembly in hepadnavirus replication requires selective encapsidation of the pregenomic RNA template and the viral polymerase by the core proteins. It has been shown that an encapsidation signal located at the 5' end of the pregenomic RNA is responsible for its interaction with the polymerase. In the present study, we have shown that a region located at the 3' periphery of the core open reading frame may interact with the viral polymerase in duck hepatitis B virus. By using an in vitro rabbit reticulocyte lysate translation system, we found that interaction of the polymerase with this region resulted in selective suppression of core mRNA translation. Insertion of this putative inhibitory sequence into the CD4 gene also led to a selective inhibition of CD4 mRNA translation in the presence of polymerase. Specific inhibition of core protein synthesis was observed in a chicken hepatoma cell line (LMH) cotransfected with core and polymerase plasmid DNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Howe AY,Tyrrell DLdoi
10.1128/JVI.70.8.5035-5042.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
5035-42issue
8eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorpora...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01893-17
更新日期:2018-02-12 00:00:00
abstract::The gene coding for the S protein, the smaller of the two envelope antigens of the duck hepatitis B virus (DHBV), is transcribed from a TATA-less promoter. In this study, we localized the promoter to a 245-bp segment of the genome that was capable of efficiently driving expression of a linked reporter gene upon transi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8813-8820.1996
更新日期:1996-12-01 00:00:00
abstract::Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is wid...
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pub_type: 杂志文章
doi:10.1128/JVI.79.5.2998-3008.2005
更新日期:2005-03-01 00:00:00
abstract::Persistent hepatitis B virus (HBV) infection is characterized by a weak and narrowly focused CD8+ T-cell response to HBV that is thought to reflect the induction of central and/or peripheral tolerance to HBV proteins in neonatal and adult onset infections, respectively. Immunotherapeutic strategies that overcome toler...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8609-8620.2002
更新日期:2002-09-01 00:00:00
abstract::Each influenza season, a set of wild-type viruses, representing one H1N1, one H3N2, and one to two influenza B isolates, are selected for inclusion in the annual seasonal influenza vaccine. In order to develop broadly reactive subtype-specific influenza vaccines, a methodology called computationally optimized broadly ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01581-17
更新日期:2017-11-30 00:00:00
abstract::Poliovirus infectious RNA can be synthesized in vitro using phage DNA-dependent RNA-polymerases. These synthetic transcripts contain several extra nucleotides at the 5' end, which are deleted during replication to generate authentic viral genomes. We removed those 5'-end extra nucleotides utilizing a hammerhead ribozy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6394-6400.2000
更新日期:2000-07-01 00:00:00
abstract::Although hepatitis A virus (HAV) is typically transmitted by the fecal-oral route, little is known of its interactions with cells of the gastrointestinal tract. We studied the replication of HAV in polarized cultures of Caco-2 cells, a human cell line which retains many differentiated functions of small intestinal epi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6476-6484.2000
更新日期:2000-07-01 00:00:00
abstract::A novel swine-origin H1N1 influenza virus [A(H1N1)pdm09 virus] caused the 2009 influenza pandemic. Most patients exhibited mild symptoms similar to seasonal influenza, but some experienced severe clinical signs and, in the worst cases, died. Such differences in symptoms are generally associated with preexisting medica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01565-12
更新日期:2013-02-01 00:00:00
abstract::Work in this laboratory previously demonstrated that the tropism of different human immunodeficiency type 1 isolates for infection of human CD4+ continuous cell lines (e.g., T-cell lines and HeLa-CD4 transformants) versus primary macrophages is associated with parallel intrinsic fusogenic specificities of the correspo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5487-5494.1996
更新日期:1996-08-01 00:00:00
abstract::T-cell epitopes on the E2 protein of rubella virus were studied by using 15 overlapping synthetic peptides covering the E2 protein sequence. The most frequently recognized epitopes on E2 were E2-4 (residues 54 to 74), with 5 of 10 tested T-cell lines responding to it. Two CD4+ cytotoxic T-cell cloned isolated from one...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6788-6793.1992
更新日期:1992-11-01 00:00:00
abstract::A mutation (asparagine 815 to serine 815) was introduced into the herpes simplex virus type 1 (HSV-1) DNA polymerase (pol). The HSV-1 pol enzyme in lysates of Saccharomyces cerevisiae cells expressing the mutant protein showed increased resistance to acyclovir triphosphate and increased sensitivity to phosphonoacetate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4913-4918.1989
更新日期:1989-11-01 00:00:00
abstract::In 1997, an H5N1 influenza virus outbreak occurred in chickens in Hong Kong, and the virus was transmitted directly to humans. Because there is limited information about the avian influenza virus reservoir in that region, we genetically characterized virus strains isolated in Hong Kong during the 1997 outbreak. We seq...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.14.6309-6315.2000
更新日期:2000-07-01 00:00:00
abstract::Elite controllers (EC) of human immunodeficiency virus type 1 (HIV-1) maintain viremia below the limit of detection without antiretroviral treatment. Virus-specific cytotoxic CD8(+) T lymphocytes are believed to play a crucial role in viral containment, but the degree of immune imprinting and compensatory mutations in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02459-08
更新日期:2009-04-01 00:00:00
abstract::Influenza A viruses (IAVs) are viral pathogens that cause epidemics and occasional pandemics of significant mortality. The generation of efficacious vaccines and antiviral drugs remains a challenge due to the rapid appearance of new influenza virus types and antigenic variants. Consequently, novel strategies for the p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01916-18
更新日期:2019-02-05 00:00:00
abstract::In gene therapy, the innate immune system is a significant barrier to the effective application of adenovirus (Ad) vectors. In kidney epithelium-derived (REC) cells, serotype 5 Ad vectors induce the expression of the chemokine CXCL10 (IP-10), a response that is dependent on NFkappaB. Compared to the parental vector Ad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14507-14515.2005
更新日期:2005-12-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) tat protein functions at a much lower level in rodent cells than in human cells. This species-specific difference in trans activation appears to be due to the lack of a functional homolog of a human cofactor for tat in rodent cells. Using HIV-1 long terminal repeat-drive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4565-4567.1990
更新日期:1990-09-01 00:00:00
abstract::We previously demonstrated that the adeno-associated virus (AAV) Rep68 and Rep78 proteins are able to nick the AAV origin of DNA replication at the terminal resolution site (trs) in an ATP-dependent manner. Using four types of modified or mutant substrates, we now have investigated the substrate requirements of Rep68 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.6096-6104.1993
更新日期:1993-10-01 00:00:00
abstract::Envelope glycoprotein E(rns) of classical swine fever virus (CSFV) has been shown to contain RNase activity and is involved in virus infection. Two short regions of amino acids in the sequence of E(rns) are responsible for RNase activity. In both regions, histidine residues appear to be essential for catalysis. They w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.151-157.1998
更新日期:1998-01-01 00:00:00
abstract::Monoclonal antibody-resistant mutants have been widely used to estimate virus mutation frequencies. We demonstrate that standard virion neutralization inevitably underestimates monoclonal antibody-resistant mutant genome frequencies of vesicular stomatitis virus, due to phenotypic masking-mixing when wild-type (wt) vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5030-5036.1989
更新日期:1989-12-01 00:00:00
abstract::We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.291-298.1978
更新日期:1978-05-01 00:00:00
abstract::An effective vaccine for AIDS may require development of novel vectors capable of eliciting long-lasting immune responses. Here we report the development and use of replication-competent and replication-defective strains of recombinant herpes simplex virus (HSV) that express envelope and Nef antigens of simian immunod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7745-7754.2000
更新日期:2000-09-01 00:00:00
abstract::Vif counteracts the host restriction factor APOBEC3G (A3G) and other APOBEC3s by preventing the incorporation of A3G into progeny virions. We previously identified Vif mutants with a dominant-negative (D/N) phenotype that interfered with the function of wild-type Vif, inhibited the degradation of A3G, and reduced the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-19
更新日期:2020-03-17 00:00:00
abstract::Transfection of the E6 and E7 genes of the high-risk human papillomaviruses (HPVs) into primary genital keratinocytes generates colonies of proliferating cells which are resistant to calcium- and serum-induced terminal differentiation. To genetically map the HPV gene(s) responsible for this cellular phenotype, we clon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3269-3279.1996
更新日期:1996-05-01 00:00:00
abstract::Herpesvirus saimiri (HVS) is the prototype gamma-2 herpesvirus, which naturally infects the squirrel monkey Saimiri sciureus, causing an asymptomatic but persistent infection. The latent phase of gamma-2 herpesviruses is characterized by their ability to persist in a dividing cell population while expressing a limited...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02171-06
更新日期:2007-04-01 00:00:00
abstract::DNA was extracted from [(3)H]thymidine-labeled Marek's disease virus (MDV) and purified by two cycles of CsCl gradient centrifugation in a fixed-angle rotor. The DNA was transcribed in vitro into (32)P-labeled complementary RNA (cRNA). MDV cRNA did not hybridize with DNA from chicken embryo fibroblast cultures or from...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.841-846.1973
更新日期:1973-10-01 00:00:00
abstract::Selecting human immunodeficiency virus (HIV) sequences for inclusion within vaccines has been a difficult problem, as circulating HIV strains evolve relentlessly and become increasingly divergent over time. We report an assessment of this divergence from three perspectives: (i) across different hosts as a function of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1637-1644.2006
更新日期:2006-02-01 00:00:00
abstract::Recombinant Epstein-Barr viruses (EBV) with a translation termination codon mutation inserted into the nuclear protein 3A (EBNA-3A) or 3C (EBNA-3C) open reading frame were generated by second-site homologous recombination. These mutant viruses were used to infect primary B lymphocytes to assess the requirement of EBNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2014-2025.1993
更新日期:1993-04-01 00:00:00
abstract::It has become evident that naturally occurring CD25(+) regulatory T cells (T(reg) cells) not only influence self-antigen specific immune response but also dampen foreign antigen specific immunity. This report extends our previous findings by demonstrating that immunity to certain herpes simplex virus (HSV) vaccines is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13082-13089.2004
更新日期:2004-12-01 00:00:00
abstract::The in vitro transcription of the genome segments of insect cytoplasmic polyhedrosis virus was examined for a possible mechanism of coordinate control. Initiation of the first transcription cycle, primed by preincubation in the absence of CTP, was synchronous for all 10 viral genome segments. The time required for the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.326-329.1982
更新日期:1982-01-01 00:00:00
abstract::Dengue virus (DENV) is a mosquito-borne flavivirus responsible for dengue disease, a major human health concern for which no specific therapies are available. Like other viruses, DENV relies heavily on the host cellular machinery for productive infection. In this study, we performed a genome-wide CRISPR-Cas9 screen us...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01751-19
更新日期:2020-03-17 00:00:00