Inhibition of simian immunodeficiency virus (SIV) replication by CD8(+) T lymphocytes from macaques immunized with live attenuated SIV.


:Characterization of immune responses induced by live attenuated simian immunodeficiency virus (SIV) strains may yield clues to the nature of protective immunity induced by this vaccine approach. We investigated the ability of CD8(+) T lymphocytes from rhesus macaques immunized with the live, attenuated SIV strain SIVmac239Deltanef or SIVmac239Delta3 to inhibit SIV replication. CD8(+) T lymphocytes from immunized animals were able to potently suppress SIV replication in autologous SIV-infected CD4(+) T cells. Suppression of SIV replication by unstimulated CD8(+) T cells required direct contact and was major histocompatibility complex (MHC) restricted. However, CD3-stimulated CD8(+) T cells produced soluble factors that inhibited SIV replication in an MHC-unrestricted fashion as much as 30-fold. Supernatants from stimulated CD8(+) T cells were also able to inhibit replication of both CCR5- and CXCR4-dependent human immunodeficiency virus type 1 (HIV-1) strains. Stimulation of CD8(+) cells with cognate cytotoxic T-lymphocyte epitopes also induced secretion of soluble factors able to inhibit SIV replication. Production of RANTES, macrophage inhibitory protein 1alpha (MIP-1alpha), or MIP-1beta from stimulated CD8(+) T cells of vaccinated animals was almost 10-fold higher than that from stimulated CD8(+) T cells of control animals. However, addition of antibodies that neutralize these beta-chemokines, either alone or in combination, only partly blocked inhibition of SIV and HIV replication by soluble factors produced by stimulated CD8(+) T cells. Our results indicate that inhibition of SIV replication by CD8(+) T cells from animals immunized with live attenuated SIV strains involves both MHC-restricted and -unrestricted mechanisms and that MHC-unrestricted inhibition of SIV replication is due principally to soluble factors other than RANTES, MIP-1alpha, and MIP-1beta.


J Virol


Journal of virology


Gauduin MC,Glickman RL,Means R,Johnson RP




Has Abstract


1998-08-01 00:00:00












  • Strain-specific role of RNAs in prion replication.

    abstract::Several lines of evidence suggest that various cofactors may be required for prion replication. PrP binds to polyanions, and RNAs were shown to promote the conversion of PrP(C) into PrP(Sc) in vitro. In the present study, we investigated strain-specific differences in RNA requirement during in vitro conversion and the...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Saá P,Sferrazza GF,Ottenberg G,Oelschlegel AM,Dorsey K,Lasmézas CI

    更新日期:2012-10-01 00:00:00

  • Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3.

    abstract::Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phen...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Vu HL,Kwon B,Yoon KJ,Laegreid WW,Pattnaik AK,Osorio FA

    更新日期:2011-06-01 00:00:00

  • Viral Characteristics Associated with Maintenance of Elite Neutralizing Activity in Chronically HIV-1 Clade C-Infected Monozygotic Pediatric Twins.

    abstract::Broad and potent neutralizing antibodies (bnAbs) with multiple epitope specificities evolve in HIV-1-infected children. Herein, we studied two antiretroviral-naive chronically HIV-1 clade C-infected monozygotic pediatric twins, AIIMS_329 and AIIMS_330, with potent plasma bnAbs. Elite plasma neutralizing activity was o...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mishra N,Makhdoomi MA,Sharma S,Kumar S,Dobhal A,Kumar D,Chawla H,Singh R,Kanga U,Das BK,Lodha R,Kabra SK,Luthra K

    更新日期:2019-08-13 00:00:00

  • Reversal of Fv-1 host range by in vitro restriction endonuclease fragment exchange between molecular clones of N-tropic and B-tropic murine leukemia virus genomes.

    abstract::We molecularly cloned unintegrated viral DNA of the BALB/c endogenous N-tropic and B-tropic murine leukemia retroviruses and in vitro passaged N-tropic Gross (passage A) murine leukemia retroviruses. Recombinant genomes were constructed in vitro by exchanging homologous restriction enzyme fragments from N- or B-tropic...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Boone LR,Myer FE,Yang DM,Ou CY,Koh CK,Roberson LE,Tennant RW,Yang WK

    更新日期:1983-10-01 00:00:00

  • Functional and topographical analyses of epitopes on the hemagglutinin (VP4) of the simian rotavirus SA11.

    abstract::An immunochemical analysis of the hemagglutinin (VP4) of the simian rotavirus SA11 was performed to better understand the structure and function of this molecule. Following immunization of mice with double-shelled virus particles and VP4-enriched fractions from CsCl gradients, a battery of anti-SA11 hybridomas was gen...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Burns JW,Greenberg HB,Shaw RD,Estes MK

    更新日期:1988-06-01 00:00:00

  • Unusual bacteriophages in Salmonella newport.

    abstract::Six phages were isolated from sewage and from a lysogenic strain. Three of them, belonging to a new morphological group, had contractile tails and elongated heads with axial ratios of 2.4:1. Two phages, possessing short tails and very long heads with axial ratios of 3.5:1, were new isolates of an extremely rare group....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ackermann HW,Petrow S,Kasatiya SS

    更新日期:1974-03-01 00:00:00

  • Infection of Chinese hamster ovary cells by pseudorabies virus.

    abstract::Chinese hamster ovary (CHO) cells have recently been used for identification of receptors for several alphaherpesviruses, including pseudorabies virus (PrV) (R. J. Geraghty, C. Krummenacher, G. H. Cohen, R. J. Eisenberg, and P. G. Spear, Science 280:1618-1620, 1998). The experiments were based on the fact that CHO cel...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Nixdorf R,Schmidt J,Karger A,Mettenleiter TC

    更新日期:1999-10-01 00:00:00

  • Parvovirus Capsid Structures Required for Infection: Mutations Controlling Receptor Recognition and Protease Cleavages.

    abstract::Parvovirus capsids are small but complex molecular machines responsible for undertaking many of the steps of cell infection, genome packing, and cell-to-cell as well as host-to-host transfer. The details of parvovirus infection of cells are still not fully understood, but the processes must involve small changes in th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Callaway HM,Feng KH,Lee DW,Allison AB,Pinard M,McKenna R,Agbandje-McKenna M,Hafenstein S,Parrish CR

    更新日期:2017-01-03 00:00:00

  • Expression of the Marek's disease virus (MDV) homolog of glycoprotein B of herpes simplex virus by a recombinant baculovirus and its identification as the B antigen (gp100, gp60, gp49) of MDV.

    abstract::A gene encoding a homolog of glycoprotein B of herpes simplex virus (gB homolog) has been identified on the Marek's disease virus (MDV) genome (L. J. N. Ross, M. Sanderson, S. D. Scott, M. M. Binns, T. Doel, and B. Milne, J. Gen. Virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Niikura M,Matsuura Y,Endoh D,Onuma M,Mikami T

    更新日期:1992-05-01 00:00:00

  • Evidence for persistent, occult infection in neonatal macaques following perinatal transmission of simian-human immunodeficiency virus SF162P3.

    abstract::To model human immunodeficiency virus (HIV) perinatal transmission, we studied infection of simian-human immunodeficiency virus (SHIV) SF162P3 in 10 pregnant Macaca nemestrina females and their offspring. Four of nine infants born to and suckled by these dams had evidence of infection, a transmission rate of 44.4% (95...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jayaraman P,Zhu T,Misher L,Mohan D,Kuller L,Polacino P,Richardson BA,Bielefeldt-Ohmann H,Anderson D,Hu SL,Haigwood NL

    更新日期:2007-01-01 00:00:00

  • Spliced mRNA encoding the murine cytomegalovirus chemokine homolog predicts a beta chemokine of novel structure.

    abstract::A viral mRNA of the late kinetic class expressed by murine cytomegalovirus (MCMV) contains an open reading frame (ORF) whose predicted protein, designated MCK-1, has homology to beta chemokines (M. R. MacDonald, X.-Y. Li, and H. W. Virgin IV, J. Virol. 71:1671-1678, 1997). The present study analyzed further the struct...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: MacDonald MR,Burney MW,Resnick SB,Virgin HW IV

    更新日期:1999-05-01 00:00:00

  • Characterization of the knob domain of the adenovirus type 5 fiber protein expressed in Escherichia coli.

    abstract::The adenovirus fiber protein is used for attachment of the virus to a specific receptor on the cell surface. Structurally, the protein consists of a long, thin shaft that protrudes from the vertex of the virus capsid and terminates in a globular domain termed the knob. To verify that the knob is the domain which inter...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Henry LJ,Xia D,Wilke ME,Deisenhofer J,Gerard RD

    更新日期:1994-08-01 00:00:00

  • DNA configuration in the core of Marek's disease virus.

    abstract::Marek's disease virus DNA appears to be wound around a central structure connecting the two inner poles of the capsid. Based on electron micrographs of Marek's disease virions, a diagram of spatial configuration of virus DNA is presented. This diagram may explain the pleomorphic character of the herpesvirus core obser...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Nazerian K

    更新日期:1974-05-01 00:00:00

  • Identification of amino acids involved in recognition by dengue virus NS3-specific, HLA-DR15-restricted cytotoxic CD4+ T-cell clones.

    abstract::The majority of T-cell clones derived from a donor who experienced dengue illness following receipt of a live experimental dengue virus type 3 (DEN3) vaccine cross-reacted with all four serotypes of dengue virus, but some were serotype specific or only partially cross-reactive. The nonstructural protein, NS3, was immu...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zeng L,Kurane I,Okamoto Y,Ennis FA,Brinton MA

    更新日期:1996-05-01 00:00:00

  • The measles virus hemagglutinin stalk: structures and functions of the central fusion activation and membrane-proximal segments.

    abstract:UNLABELLED:The measles virus (MeV) membrane fusion apparatus consists of a fusion protein trimer and an attachment protein tetramer. To trigger membrane fusion, the heads of the MeV attachment protein, hemagglutinin (H), bind cellular receptors while the 96-residue-long H stalk transmits the triggering signal. Structur...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Navaratnarajah CK,Kumar S,Generous A,Apte-Sengupta S,Mateo M,Cattaneo R

    更新日期:2014-06-01 00:00:00

  • Cell penetration and trafficking of polyomavirus.

    abstract::The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfi...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gilbert JM,Goldberg IG,Benjamin TL

    更新日期:2003-02-01 00:00:00

  • Immunization with a single major histocompatibility complex class I-restricted cytotoxic T-lymphocyte recognition epitope of herpes simplex virus type 2 confers protective immunity.

    abstract::We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8(+) cytotoxic T-lymphocyte (CTL) response directed against a single major histocompatibility complex class I-restricted CTL recognition epitope. We generated a recombinant...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Blaney JE Jr,Nobusawa E,Brehm MA,Bonneau RH,Mylin LM,Fu TM,Kawaoka Y,Tevethia SS

    更新日期:1998-12-01 00:00:00

  • Enumeration and functional evaluation of virus-specific CD4+ and CD8+ T cells in lymphoid and peripheral sites of coxsackievirus B3 infection.

    abstract::Previous studies have suggested that coxsackievirus B (CVB) activates CD8(+) T cells in vivo, but the extent of this activation and the antigen specificity of the CD8(+) T cells remain uncertain. Furthermore, CVB-induced CD4(+) T-cell responses have not been carefully investigated. Herein, we evaluate CD8(+) and CD4(+...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kemball CC,Harkins S,Whitton JL

    更新日期:2008-05-01 00:00:00

  • Interferon-induced human protein MxA is a GTPase which binds transiently to cellular proteins.

    abstract::MxA is an abundant and ubiquitous cytoplasmic protein induced by alpha/beta interferon in human cells. Upon full induction, it can constitute 0.5 to 1% of cytosolic proteins. MxA can bind elements of the cytoskeleton, such as actin and tubulins, and several larger cellular proteins. However, these protein-protein inte...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Horisberger MA

    更新日期:1992-08-01 00:00:00

  • Relative replication fitness of a high-level 3'-azido-3'-deoxythymidine-resistant variant of human immunodeficiency virus type 1 possessing an amino acid deletion at codon 67 and a novel substitution (Thr-->Gly) at codon 69.

    abstract::The combination of an amino acid deletion at codon 67 (delta 67) and Thr-to-Gly change at codon 69 (T69G) in the reverse transcriptase (RT) of human immunodeficiency virus type 1 (HIV-1) is associated with high-level resistance to multiple RT inhibitors. To determine the relative contributions of the delta 67 and T69G...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Imamichi T,Berg SC,Imamichi H,Lopez JC,Metcalf JA,Falloon J,Lane HC

    更新日期:2000-12-01 00:00:00

  • A poxvirus host range protein, CP77, binds to a cellular protein, HMG20A, and regulates its dissociation from the vaccinia virus genome in CHO-K1 cells.

    abstract::Vaccinia virus does not grow in Chinese hamster ovary (CHO-K1) cells in the absence of a viral host range factor, cowpox protein CP77. In this study, CP77 was fused to the C terminus of green fluorescence protein (GFP-CP77) and a series of nested deletion mutants of GFP-CP77 was constructed for insertion into a vaccin...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hsiao JC,Chao CC,Young MJ,Chang YT,Cho EC,Chang W

    更新日期:2006-08-01 00:00:00

  • Modeling the acute and chronic phases of Theiler murine encephalomyelitis virus infection.

    abstract::Theiler murine encephalomyelitis virus (TMEV) infection of a mouse's central nervous system is biphasic: first the virus infects motor neurons (acute phase), and this is followed by a chronic phase in which the virus infects glial cells (primarily microglia and macrophages [M]) of the spinal cord white matter, leading...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Zhang J,Lipton HL,Perelson AS,Dahari H

    更新日期:2013-04-01 00:00:00

  • Inhibition of Beta interferon induction by severe acute respiratory syndrome coronavirus suggests a two-step model for activation of interferon regulatory factor 3.

    abstract::Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus termed SARS-CoV. We and others have previously shown that the replication of SARS-CoV can be suppressed by exogenously added interferon (IFN), a cytokine which is normally synthesized by cells as a reaction to virus infection. Here, we demonstra...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Spiegel M,Pichlmair A,Martínez-Sobrido L,Cros J,García-Sastre A,Haller O,Weber F

    更新日期:2005-02-01 00:00:00

  • Substitutions at amino acid positions 143, 148, and 155 of HIV-1 integrase define distinct genetic barriers to raltegravir resistance in vivo.

    abstract::Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Fransen S,Gupta S,Frantzell A,Petropoulos CJ,Huang W

    更新日期:2012-07-01 00:00:00

  • The promoter-regulatory region of the major immediate-early gene of human cytomegalovirus responds to T-lymphocyte stimulation and contains functional cyclic AMP-response elements.

    abstract::Prior studies have demonstrated that a small proportion of blood lymphocytes from patients with human cytomegalovirus (HCMV) infection express only the viral immediate-early (IE) genes (L. Einhorn and A. Ost, J. Infect. Dis. 149:207-214, 1984; G. P. A. Rice, R. D. Schrier, and M. B. A. Oldstone, Proc. Natl. Acad. Sci....

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hunninghake GW,Monick MM,Liu B,Stinski MF

    更新日期:1989-07-01 00:00:00

  • Neutralization profiles of primary human immunodeficiency virus type 1 isolates in the context of coreceptor usage.

    abstract::Most strains of human immunodeficiency virus type 1 (HIV-1) which have only been carried in vitro in peripheral blood mononuclear cells (primary isolates) can be neutralized by antibodies, but their sensitivity to neutralization varies considerably. To study the parameters that contribute to the differential neutraliz...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cecilia D,KewalRamani VN,O'Leary J,Volsky B,Nyambi P,Burda S,Xu S,Littman DR,Zolla-Pazner S

    更新日期:1998-09-01 00:00:00

  • Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro.

    abstract::Normal replication of Moloney murine leukemia virus (MoMLV) requires the integration of a DNA copy of the viral RNA genome into a chromosome of the host. In this work, we characterize the DNA sequences at the ends of the linear proviral precursor that are required for integration in the presence of MoMLV integration p...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bushman FD,Craigie R

    更新日期:1990-11-01 00:00:00

  • RIG-I-Mediated STING Upregulation Restricts Herpes Simplex Virus 1 Infection.

    abstract:UNLABELLED:STING has emerged in recent years as a key player in orchestrating innate immune responses to cytosolic DNA and RNA derived from pathogens. However, the regulation of STING still remains poorly defined. In the present study, we investigated the mechanism of the regulation of STING expression in relation to t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Liu Y,Goulet ML,Sze A,Hadj SB,Belgnaoui SM,Lababidi RR,Zheng C,Fritz JH,Olagnier D,Lin R

    更新日期:2016-09-29 00:00:00

  • Breast Milk of HIV-Positive Mothers Has Potent and Species-Specific In Vivo HIV-Inhibitory Activity.

    abstract:UNLABELLED:Despite the nutritional and health benefits of breast milk, breast milk can serve as a vector for mother-to-child HIV transmission. Most HIV-infected infants acquire HIV through breastfeeding. Paradoxically, most infants breastfed by HIV-positive women do not become infected. This is potentially attributed t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wahl A,Baker C,Spagnuolo RA,Stamper LW,Fouda GG,Permar SR,Hinde K,Kuhn L,Bode L,Aldrovandi GM,Garcia JV

    更新日期:2015-11-01 00:00:00

  • Efficient Inhibition of Avian and Seasonal Influenza A Viruses by a Virus-Specific Dicer-Substrate Small Interfering RNA Swarm in Human Monocyte-Derived Macrophages and Dendritic Cells.

    abstract::Influenza A viruses (IAVs) are viral pathogens that cause epidemics and occasional pandemics of significant mortality. The generation of efficacious vaccines and antiviral drugs remains a challenge due to the rapid appearance of new influenza virus types and antigenic variants. Consequently, novel strategies for the p...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Jiang M,Österlund P,Westenius V,Guo D,Poranen MM,Bamford DH,Julkunen I

    更新日期:2019-02-05 00:00:00