Generation of a complete single-gene knockout bacterial artificial chromosome library of cowpox virus and identification of its essential genes.

abstract：:The reasons for poor CD4+ T-cell recovery in some human immunodeficiency virus (HIV)-infected subjects despite effective highly active antiretroviral therapy (HAART) remain unclear. We recently reported that CXCR4-using (X4) HIV-1 could be gradually selected in cellular reservoirs during sustained HAART. Because of th...

journal_title：Journal of virology

authors： Delobel P,Nugeyre MT,Cazabat M,Sandres-Sauné K,Pasquier C,Cuzin L,Marchou B,Massip P,Cheynier R,Barré-Sinoussi F,Izopet J,Israël N

更新日期：2006-10-01 00:00:00

abstract：:Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced host cell apoptosis has been established, the underlying molecular mechanism is still unclear. We report here that IBDV viral protein 5 (VP5) is a major apoptosis induce...

journal_title：Journal of virology

authors： Li Z,Wang Y,Xue Y,Li X,Cao H,Zheng SJ

更新日期：2012-02-01 00:00:00

abstract：:Marek's disease (MD), a highly infectious disease caused by an oncogenic herpesvirus, is one of the few herpesvirus diseases against which live attenuated vaccines are used as the main strategy for control. We have constructed bacterial artificial chromosomes (BACs) of the CVI988 (Rispens) strain of the virus, the mos...

journal_title：Journal of virology

authors： Petherbridge L,Howes K,Baigent SJ,Sacco MA,Evans S,Osterrieder N,Nair V

更新日期：2003-08-01 00:00:00

abstract：:An in situ molecular hybridization system which will detect retrovirus RNA in the cytoplasm of individual virus-infected cells has been developed. The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus ...

journal_title：Journal of virology

authors： Kaufman SL,Gallo RC,Miller NR

更新日期：1979-05-01 00:00:00

abstract：:Inhibition of RNA synthesis of the LH strain of type 3 poliovirus at the restrictive temperature occurs concurrently with the breakdown of noncapsid virus-specific peptide 2. This finding, along with other reported data (B.D. Korant, In Proteases and Biological Control, p. 621-644, 1975), suggests that noncapsid virus...

journal_title：Journal of virology

authors： Bowles SA,Tershak DR

更新日期：1978-08-01 00:00:00

abstract：UNLABELLED:Hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTLs) are critical in eliminating infection. We developed an animal model in which HBV-infected human hepatocytes are targeted by HBV-specific CTLs. After HBV inoculation in human hepatocyte-transplanted herpes simplex virus type-1 thymidine kinase-NO...

journal_title：Journal of virology

authors： Uchida T,Hiraga N,Imamura M,Tsuge M,Abe H,Hayes CN,Aikata H,Ishida Y,Tateno C,Yoshizato K,Ohdan H,Murakami K,Chayama K

更新日期：2015-10-01 00:00:00

abstract：:The immunosuppressant drug rapamycin blocks progression of the cell cycle at G1 in mammalian cells and yeast. We recently showed that rapamycin inhibits both in vitro and in vivo cap-dependent, but not cap-independent, translation. This inhibition is causally related to reduced phosphorylation and consequent activatio...

journal_title：Journal of virology

authors： Beretta L,Svitkin YV,Sonenberg N

更新日期：1996-12-01 00:00:00

abstract：:Prior to the introduction of live-attenuated vaccines, mumps virus (MuV) was the leading cause of virus-induced meningitis. Although vaccination has been effective at controlling the disease, the use of insufficiently attenuated strains has been associated with high rates of aseptic meningitis in vaccinees. The molecu...

journal_title：Journal of virology

authors： Lemon K,Rima BK,McQuaid S,Allen IV,Duprex WP

更新日期：2007-08-01 00:00:00

abstract：:The signaling lymphocyte activation molecule F1 (SLAMF1) is both a microbial sensor and entry receptor for measles virus (MeV). Herein, we describe a new role for SLAMF1 to mediate MeV endocytosis that is in contrast with the alternative, and generally accepted, model that MeV genome enters cells only after fusion at ...

journal_title：Journal of virology

authors： Gonçalves-Carneiro D,McKeating JA,Bailey D

更新日期：2017-03-13 00:00:00

abstract：:Mutations at amino acids 143, 148, and 155 in HIV-1 integrase (IN) define primary resistance pathways in subjects failing raltegravir (RAL)-containing treatments. Although each pathway appears to be genetically distinct, shifts in the predominant resistant virus population have been reported under continued drug press...

journal_title：Journal of virology

authors： Fransen S,Gupta S,Frantzell A,Petropoulos CJ,Huang W

更新日期：2012-07-01 00:00:00

abstract：:Heat shock protein 70 (Hsp70) is incorporated within the membrane of primate lentiviral virions. Here we demonstrate that Hsp70 is also incorporated into oncoretroviral virions and that it remains associated with membrane-stripped human immunodeficiency virus type 1 (HIV-1) virion cores. To determine if Hsp70 promotes...

journal_title：Journal of virology

authors： Gurer C,Höglund A,Höglund S,Luban J

更新日期：2005-05-01 00:00:00

abstract：:Herpes simplex virus DNA replication proteins localize in characteristic patterns corresponding to viral DNA replication structures in the infected cell nucleus. The intranuclear spatial organization of the HSV DNA replication structures and the factors regulating their nuclear location remain to be defined. We have u...

journal_title：Journal of virology

authors： de Bruyn Kops A,Knipe DM

更新日期：1994-06-01 00:00:00

abstract：:The nuclear egress complex (NEC) is required for efficient translocation of newly synthesized herpesvirus nucleocapsids from the nucleus to the cytosol. It consists of the type II membrane protein pUL34 which interacts with pUL31 at the inner nuclear membrane (INM). To map regions within pUL34 required for nuclear mem...

journal_title：Journal of virology

authors： Paßvogel L,Trübe P,Schuster F,Klupp BG,Mettenleiter TC

更新日期：2013-04-01 00:00:00

abstract：:Polymerase basic protein 2 (PB2), a component of the influenza virus polymerase complex, when expressed alone from cloned cDNA in the absence of other influenza virus proteins, is transported into the nucleus. In this study, we have examined the nuclear translocation signal of PB2 by making deletions and mutations in ...

journal_title：Journal of virology

authors： Mukaigawa J,Nayak DP

更新日期：1991-01-01 00:00:00

abstract：:Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected...

journal_title：Journal of virology

authors： Chesebro B,Wehrly K

更新日期：1988-10-01 00:00:00

abstract：:Type I interferon is induced shortly following viral infection and represents a first line of host defense against a majority of viral pathogens. Not surprisingly, both replication and latency of gammaherpesviruses, ubiquitous cancer-associated pathogens, are attenuated by type I interferon, although the mechanism of ...

journal_title：Journal of virology

authors： Mounce BC,Mboko WP,Kanack AJ,Tarakanova VL

更新日期：2014-02-01 00:00:00

abstract：:Dengue virus (DENV) is an important human pathogen, especially in the tropical and subtropical parts of the world, causing considerable morbidity and mortality. DENV replication occurs in the cytoplasm; however, a high proportion of nonstructural protein 5 (NS5), containing methyltransferase (MTase) and RNA-dependent ...

journal_title：Journal of virology

authors： Kumar A,Bühler S,Selisko B,Davidson A,Mulder K,Canard B,Miller S,Bartenschlager R

更新日期：2013-04-01 00:00:00

abstract：:The influenza virus NS1 protein inhibits the nuclear export of a spliced viral mRNA, NS2 mRNA (F. V. Alonso-Caplen, M. E. Nemeroff, Y. Qiu, and R. M. Krug, Genes Dev. 6:255-267, 1992). To identify the sequence in NS2 mRNA that is recognized by the NS1 protein, we developed a gel shift assay for the formation of specif...

journal_title：Journal of virology

authors： Qiu Y,Krug RM

更新日期：1994-04-01 00:00:00

abstract：:Treatment of influenza virus and its purified hemagglutining with glycosidases from Diplococcus pneumoniae, which included beta-galactosidase, beta-N-acetylglucosminidase, and endoglycosidase D, released amino and neutral sugars from the virus and these as well as large oligosaccharides from the purified hemagglutinin...

journal_title：Journal of virology

authors： Collins JK,Knight CA

更新日期：1978-07-01 00:00:00

abstract：:The relationship between immunosenescence and the host response to virus infection is poorly understood at the molecular level. Two different patterns of pulmonary host responses to virus were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that ...

journal_title：Journal of virology

authors： Baas T,Roberts A,Teal TH,Vogel L,Chen J,Tumpey TM,Katze MG,Subbarao K

更新日期：2008-10-01 00:00:00

abstract：:Severe complications of Zika virus (ZIKV) infection might be caused by inflammation, but how ZIKV induces proinflammatory cytokines is not understood. In this study, we show opposite regulatory effects of the ZIKV NS5 protein on interferon (IFN) signaling. Whereas ZIKV and its NS5 protein were potent suppressors of ty...

journal_title：Journal of virology

authors： Chaudhary V,Yuen KS,Chan JF,Chan CP,Wang PH,Cai JP,Zhang S,Liang M,Kok KH,Chan CP,Yuen KY,Jin DY

更新日期：2017-06-26 00:00:00

abstract：:Activated microglia and astroglia are known to be involved in a variety of neurodegenerative diseases, including prion diseases. In the present experiments, we studied activation of astroglia and microglia after intraocular scrapie infection in transgenic mice expressing prion protein (PrP) in multiple cell types (tg7...

journal_title：Journal of virology

authors： Kercher L,Favara C,Striebel JF,LaCasse R,Chesebro B

更新日期：2007-10-01 00:00:00

abstract：:SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...

journal_title：Journal of virology

authors： Trautz B,Pierini V,Wombacher R,Stolp B,Chase AJ,Pizzato M,Fackler OT

更新日期：2016-11-14 00:00:00

abstract：:Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each s...

journal_title：Journal of virology

authors： Lee H,Liu Y,Mejia E,Paul AV,Wimmer E

更新日期：2006-11-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the complex cis-acting oriLyt region, which spans nearly 3 kb. DNA synthesis requires six core proteins together with UL84 and IE2. Previously, two essential regions were identified within oriLyt. Essential region I (nucleotides [nt] 92209 to 92573) ca...

journal_title：Journal of virology

authors： Colletti KS,Smallenburg KE,Xu Y,Pari GS

更新日期：2007-07-01 00:00:00

abstract：:Human parechoviruses (HPeV), members of the Parechovirus genus of Picornaviridae, are frequent pathogens but have been comparatively poorly studied, and little is known of their diversity, evolution, and molecular biology. To increase the amount of information available, we have analyzed 7 HPeV strains isolated in Cal...

journal_title：Journal of virology

authors： Al-Sunaidi M,Williams CH,Hughes PJ,Schnurr DP,Stanway G

更新日期：2007-01-01 00:00:00

abstract：:A more complete assessment of ovine prion strain diversity will be achieved by complementing biological strain typing in conventional and ovine PrP transgenic mice with a biochemical analysis of the resultant PrPSc. This will provide a correlation between ovine prion strain phenotype and the molecular nature of differ...

journal_title：Journal of virology

authors： Thackray AM,Hopkins L,Spiropoulos J,Bujdoso R

更新日期：2008-11-01 00:00:00

abstract：:In 1997, an H5N1 influenza virus outbreak occurred in chickens in Hong Kong, and the virus was transmitted directly to humans. Because there is limited information about the avian influenza virus reservoir in that region, we genetically characterized virus strains isolated in Hong Kong during the 1997 outbreak. We seq...

journal_title：Journal of virology

authors： Hoffmann E,Stech J,Leneva I,Krauss S,Scholtissek C,Chin PS,Peiris M,Shortridge KF,Webster RG

更新日期：2000-07-01 00:00:00

abstract：:A widespread porcine epidemic diarrhea virus (PEDV) occurred in southern China during 2010 to 2012. A virulent field PEDV strain, GD-B, was isolated from a sucking piglet suffering from severe diarrhea in Guangdong, China. We sequenced and analyzed the complete genome of strain GD-B, which will promote a better unders...

journal_title：Journal of virology

authors： Luo Y,Zhang J,Deng X,Ye Y,Liao M,Fan H

更新日期：2012-09-01 00:00:00

abstract：:The cytoplasmic assembly of vaccinia virus is reversibly blocked by the antibiotic rifampin, leading to the accumulation of partially membrane-delineated rifampin bodies in infected cells. Rifampin-resistant vaccinia virus mutants have point mutations in the D13L gene, which is controlled by a late promoter and expres...

journal_title：Journal of virology

authors： Sodeik B,Griffiths G,Ericsson M,Moss B,Doms RW

更新日期：1994-02-01 00:00:00