Abstract:
:Transcript levels from the P5 promoter of adeno-associated virus type 2 (AAV) are negatively regulated by the AAV Rep78 and Rep68 proteins in the absence of helper virus. We have identified a Rep-responsive negative cis element of the P5 promoter between the P5 TATA box and transcription start site by using 5' and 3' deletions of the P5 promoter fused to the chloramphenicol acetyltransferase gene. This element contains four imperfect GAGC repeats similar to the Rep recognition sequences (RRSs) in the AAV inverted terminal repeats and in the AAV preferred integration locus in chromosome 19. Band shift analyses showed that human 293 cell nuclear extracts containing Rep68 or Rep68/K340H, a putative nucleoside triphosphate (NTP)-binding-site mutant of Rep68, formed Rep-specific complexes with this P5 RRS DNA. Within the P5 RRS, mutation of a cytosine at position 273 in the AAV sequence to guanine abolished Rep68 binding to the DNA. A mutation in the P5 RRS within a full-length AAV genome, which abolished Rep binding, resulted in a 40 to 50% reduction in the ability of wild-type Rep68 to inhibit the accumulation of P5 transcripts in vivo. In contrast, the Rep68/K340H mutant was unable to down-regulate this mutated promoter. These results indicate that there are at least two mechanisms involved in the negative regulation of P5 transcript levels by Rep68; one involves Rep68 binding to the P5 RRS, and another requires the region of Rep68 containing the consensus NTP-binding motif. Furthermore, our studies of AAV genomes containing mutated RRS- and/or YY1-binding elements suggest that transcription factor YY1 binding to the transcription start site of P5 interferes with Rep68 repression of the P5 promoter.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kyöstiö SR,Wonderling RS,Owens RAdoi
10.1128/JVI.69.11.6787-6796.1995subject
Has Abstractpub_date
1995-11-01 00:00:00pages
6787-96issue
11eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Neurotropic herpesviruses depend on long-distance axon transport for the initial establishment of latency in peripheral ganglia (retrograde transport) and for viral spread in axons to exposed body surfaces following reactivation (anterograde transport). Images of neurons infected with herpes simplex virus type 1 (HSV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01296-10
更新日期:2010-12-01 00:00:00
abstract:UNLABELLED:We have recently shown in both herpesviruses and phages that packaged viral DNA creates a pressure of tens of atmospheres pushing against the interior capsid wall. For the first time, using differential scanning microcalorimetry, we directly measured the energy powering the release of pressurized DNA from th...
journal_title:Journal of virology
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abstract::Serial passage of reovirus temperature-sensitive (ts) mutant C(447) produced by passage 9 (P9) a heavily defective population of virus from which the double-stranded RNA genomic segments L(1), L(3), and M(1) were largely missing. Viral cores obtained from this P9 population were heterogeneous with respect to buoyant d...
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pub_type: 杂志文章
doi:10.1128/JVI.23.2.250-262.1977
更新日期:1977-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.80.7.3523-3531.2006
更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4494-4497.1991
更新日期:1991-08-01 00:00:00
abstract::Dendritic cells (DC) are believed to play an important role in the initiation of innate and adaptive immune responses to infection, including respiratory tract infections, where respiratory DC (RDC) perform this role. In this report, we examined the susceptibilities of isolated murine RDC to influenza virus infection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02367-07
更新日期:2008-05-01 00:00:00
abstract::Differentiating infected from vaccinated animals (DIVA) strategies have been central enabling techniques in several successful viral disease elimination programs. However, owing to their long and uncertain development process, no DIVA-compatible vaccines are available for many important diseases. We report herein a ne...
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pub_type: 杂志文章
doi:10.1128/JVI.01573-19
更新日期:2020-02-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.2022-2026.1996
更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.10168-10171.2003
更新日期:2003-09-01 00:00:00
abstract::Using a yeast two-hybrid screen of a T-cell cDNA library to identify cellular proteins that bind to the human immunodeficiency virus type 2 (HIV-2) Gag polyprotein, we identified PRP4, a serine-threonine protein kinase. Specific interaction of PRP4 and HIV-2 Gag was confirmed in in vitro and in vivo assays. The intera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.20.11303-11312.2004
更新日期:2004-10-01 00:00:00
abstract::The human cytomegalovirus (HCMV) tegument protein pp71, encoded by gene UL82, stimulates viral immediate-early (IE) transcription. pp71 interacts with the cellular protein hDaxx at nuclear domain 10 (ND10) sites, resulting in the reversal of hDaxx-mediated repression of viral transcription. We demonstrate that pp71 di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01215-08
更新日期:2008-12-01 00:00:00
abstract::Herpes simplex virus (HSV) codes for several envelope glycoproteins, including glycoprotein G-2 (gG-2) of HSV type 2 (HSV-2), which are dispensable for replication in cell culture. However, clinical isolates which are deficient in such proteins occur rarely. We describe here five clinical HSV-2 isolates which were fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.12.9796-9802.1999
更新日期:1999-12-01 00:00:00
abstract::The phylogeny of reptilian herpesviruses (HVs) relative to mammalian and avian HVs was investigated by using available gene sequences and by alignment of encoded amino acid sequences and derivation of trees by maximum-likelihood and Bayesian methods. Phylogenetic loci were obtained for green turtle HV (GTHV) primarily...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.725-731.2005
更新日期:2005-01-01 00:00:00
abstract::tsAF8 cells are temperature-sensitive (ts) mutants of BHK-21 cells that arrest at the nonpermissive temperature in the G1 phase of the cell cycle. When made quiescent by serum restriction, they can be stimulated to enter the S phase by 10% serum at 34 degrees C, but not at 40.6 degrees C. Infection by adenovirus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.3.982-986.1981
更新日期:1981-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
更新日期:1990-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.677-687.1978
更新日期:1978-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6541-6546.1992
更新日期:1992-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2508-2514.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.872-877.1980
更新日期:1980-12-01 00:00:00
abstract::Cells of Mus minutoides, an African pygmy mouse of the subgenus Nannomys, are susceptible to ecotropic Moloney and Friend mouse leukemia viruses (MLVs) but not to AKV-type MLVs. Transfected MA139 ferret cells expressing the mCAT-1 cell surface receptor, with the minCAT-1 substitutions K222Q and V233L, did not restrict...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00780-07
更新日期:2007-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
abstract::Since human immunodeficiency virus (HIV)-specific cell-mediated immune (CMI) responses are critical in the early control and resolution of HIV infection and correlate with postchallenge outcomes in rhesus macaque challenge experiments, we sought to identify a plasmid DNA (pDNA) vaccine design capable of eliciting robu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00055-07
更新日期:2007-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.229-237.1981
更新日期:1981-07-01 00:00:00
abstract::The presence of a high number of activated T cells in the bloodstream and spontaneous proliferation of peripheral blood mononuclear cells in vitro are striking characteristics of human T-cell leukemia virus type I (HTLV-I) infection. The HTLV-I regulatory protein Tax and the envelope protein gp46 have been implicated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3134-3141.1993
更新日期:1993-06-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) vectors that express heterologous antigens have shown promise as vaccines in preclinical studies. The efficacy of VSV-based vaccines can be improved by engineering vectors that enhance innate immune responses. We previously generated a VSV vaccine vector that incorporates two enhancing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02898-13
更新日期:2014-01-01 00:00:00