Attachment of avidin-coupled spheres to linear and circular forms of mengovirus double-stranded RNA.

abstract：:A double-isotopic label difference analysis of polyacrylamide gels has been used to distinguish between cellular and viral protein accumulation in infected cells and to quantify the kinetics of accumulation of viral polypeptides. This technique, coupled with the determination of total radioactive amino acid incorporat...

journal_title：Journal of virology

authors： Hightower LE,Bratt MA

更新日期：1974-04-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) infection has been shown to activate the mTORC1 signaling pathway. However, the phosphorylation of mTORC1 targets is differentially sensitive to the mTORC1 inhibitor rapamycin, and the drug inhibits HCMV replication to a modest extent. Using Torin1, a newly developed inhibitor that targets...

journal_title：Journal of virology

authors： Moorman NJ,Shenk T

更新日期：2010-05-01 00:00:00

abstract：UNLABELLED:Susceptibility or resistance to prion infection in humans and animals depends on single prion protein (PrP) amino acid substitutions in the host, but the agent's modulating role has not been well investigated. Compared to disease incubation times in wild-type homozygous ARQ/ARQ (where each triplet represents...

journal_title：Journal of virology

authors： Langeveld JP,Jacobs JG,Hunter N,van Keulen LJ,Lantier F,van Zijderveld FG,Bossers A

更新日期：2015-10-28 00:00:00

abstract：:Efforts to develop adenovirus vectors suitable for genetic interventions in humans have identified three major limitations of the most frequently used vector prototype, human adenovirus serotype 5 (Ad5). These limitations--widespread preexisting anti-Ad5 immunity in humans, the high rate of transduction of normal nont...

journal_title：Journal of virology

authors： Belousova N,Mikheeva G,Xiong C,Soghomonian S,Young D,Le Roux L,Naff K,Bidaut L,Wei W,Li C,Gelovani J,Krasnykh V

更新日期：2010-10-01 00:00:00

abstract：:The susceptibility to tumors induced by raf and raf/myc retroviruses was investigated in BALB/c, C57BL/6, (BALB/c x C57BL/6)F1 and (BALB/c x C57BL/6) backcross mice. Newborn mice were susceptible to neoplasms generated by both viruses, but resistance to raf-induced leukemia developed rapidly in all mice as they mature...

journal_title：Journal of virology

authors： Klinken SP,Hartley JW,Fredrickson TN,Rapp UR,Morse HC 3rd

更新日期：1989-05-01 00:00:00

abstract：:Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...

journal_title：Journal of virology

authors： Sharpe AH,Fields BN

更新日期：1981-04-01 00:00:00

abstract：:Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t...

journal_title：Journal of virology

authors： Haffar OK,Nakamura GR,Berman PW

更新日期：1990-06-01 00:00:00

abstract：:An infectious cDNA clone of a genotype 3 strain of hepatitis E virus adapted to growth in HepG2/C3A human hepatoma cells was constructed. This virus was unusual in that the hypervariable region of the adapted virus contained a 171-nucleotide insertion that encoded 58 amino acids of human S17 ribosomal protein. Analyse...

journal_title：Journal of virology

authors： Shukla P,Nguyen HT,Faulk K,Mather K,Torian U,Engle RE,Emerson SU

更新日期：2012-05-01 00:00:00

abstract：:We have isolated a new type of temperature-sensitive mutant of simian virus 40 (SV40) that is capable of productive infection in permissive cells but not of maintenance of viral DNA integration in transformed cells at the conditional temperature. Virus development is induced when cells transformed by this mutant are s...

journal_title：Journal of virology

authors： Rothschild H,Lockwood MP

更新日期：1976-08-01 00:00:00

abstract：:Bacteriophage with linear, double-stranded DNA genomes package DNA into preassembled protein shells called procapsids. Located at one vertex in the procapsid is a portal complex composed of a ring of 12 subunits of portal protein. The portal complex serves as a docking site for the DNA packaging enzymes, a conduit for...

journal_title：Journal of virology

authors： Moore SD,Prevelige PE Jr

更新日期：2002-10-01 00:00:00

abstract：UNLABELLED:In vitro, infection of polarized human intestinal epithelial cells by coxsackievirus B3 (CVB3) depends on virus interaction with decay-accelerating factor (DAF), a receptor expressed on the apical cell surface. Although mice are highly susceptible to CVB3 infection when virus is delivered by intraperitoneal ...

journal_title：Journal of virology

authors： Pan J,Zhang L,Odenwald MA,Shen L,Turner JR,Bergelson JM

更新日期：2015-04-01 00:00:00

abstract：:Heterologous viral infections have been shown to impact the preexisting memory CD8(+)-T-cell repertoire. Bacterial superantigens are products of common human pathogenic bacteria, including staphylococci and streptococci, that are potent T-cell-stimulatory molecules. In this report, we show that exposure to staphylococ...

journal_title：Journal of virology

authors： Huang CC,Shah S,Nguyen P,Altman JD,Blackman MA

更新日期：2002-07-01 00:00:00

abstract：:Herpes simplex virus establishes latent infections in the nuclei of sensory neurons. These infections are characterized by the abundant expression of a series of 5' coterminal transcripts termed the latency-associated transcripts (LATs). Available evidence indicates that LAT expression is specifically regulated in lat...

journal_title：Journal of virology

authors： Frazier DP,Cox D,Godshalk EM,Schaffer PA

更新日期：1996-11-01 00:00:00

abstract：:The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol. 24:534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue o...

journal_title：Journal of virology

authors： Vogel T,Gluzman Y,Winocour E

更新日期：1977-11-01 00:00:00

abstract：:Upon stimulation with alpha/beta interferon, rat cells synthesize three Mx proteins. Sequence analysis of corresponding cDNAs reveals that these three proteins are derived from three distinct genes. One of the rat cDNAs is termed Mx1 because it is most closely related to the mouse Mx1 cDNA and because it codes for a n...

journal_title：Journal of virology

authors： Meier E,Kunz G,Haller O,Arnheiter H

更新日期：1990-12-01 00:00:00

abstract：:Murine models have suggested that CD8+ T-cell responses peak early in acute viral infections and are not sustained, but no evidence for humans has been available. To address this, we longitudinally analyzed the CD8+ T-cell response to human parvovirus B19 in acutely infected individuals. We observed striking CD8+ T-ce...

journal_title：Journal of virology

authors： Norbeck O,Isa A,Pöhlmann C,Broliden K,Kasprowicz V,Bowness P,Klenerman P,Tolfvenstam T

更新日期：2005-09-01 00:00:00

abstract：:The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...

journal_title：Journal of virology

authors： Johnson PA,Rosner MR

更新日期：1986-06-01 00:00:00

abstract：:An alternatively spliced form of c-myb exists that encodes an additional 120 amino acids in chicken and 121 amino acids in human and mouse. These amino acids are encoded by an additional exon, termed exon 9A. This exon is not present in v-myb, and proteins containing these amino acids have never been tested for oncoge...

journal_title：Journal of virology

authors： Woo CH,Sopchak L,Lipsick JS

更新日期：1998-08-01 00:00:00

abstract：:HTG2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. In addition, it has been noted that the core of the virus remains in the "immature" form as a result of ...

journal_title：Journal of virology

authors： Demsey A,Collins F,Kawka D

更新日期：1980-12-01 00:00:00

abstract：:Immunogenic regions of the gp41 transmembrane protein of human immunodeficiency virus type 1 (HIV-1) were previously mapped by examining polyclonal sera from HIV-infected patients and rodent polyclonal and monoclonal antibodies (MAbs) to peptides of gp41. To define the epitopes within these regions to which infected h...

journal_title：Journal of virology

authors： Xu JY,Gorny MK,Palker T,Karwowska S,Zolla-Pazner S

更新日期：1991-09-01 00:00:00

abstract：:The need to replace rabies immune globulin (RIG) as an essential component of rabies postexposure prophylaxis is widely acknowledged. We set out to discover a unique combination of human monoclonal antibodies (MAbs) able to replace RIG. Stringent criteria concerning neutralizing potency, affinity, breadth of neutraliz...

journal_title：Journal of virology

authors： Bakker AB,Marissen WE,Kramer RA,Rice AB,Weldon WC,Niezgoda M,Hanlon CA,Thijsse S,Backus HH,de Kruif J,Dietzschold B,Rupprecht CE,Goudsmit J

更新日期：2005-07-01 00:00:00

abstract：:The expression of reporter genes driven by the same human elongation factor 1alpha (EF1alpha) promoter in murine leukemia virus (MLV)- and human immunodeficiency virus type 1 (HIV-1)-based vectors was studied in either transfected or virally transduced cells. The HIV-1 vectors consistently expressed 3 to 10 times high...

journal_title：Journal of virology

authors： Zaiss AK,Son S,Chang LJ

更新日期：2002-07-01 00:00:00

abstract：:Two mutant strains (vi12 and vi13) of herpes simplex virus that contain insertion mutations in the sequences that encode the DNA-binding domain of viral regulatory protein ICP4 were generated. Both mutations disrupted specific DNA binding and resulted in transcriptionally inactive molecules; however, the ability of th...

journal_title：Journal of virology

authors： Shepard AA,DeLuca NA

更新日期：1991-01-01 00:00:00

abstract：:Recognition of viruses by germ line-encoded pattern recognition receptors of the innate immune system is essential for rapid production of type I interferon (IFN) and early antiviral defense. We investigated the mechanisms of viral recognition governing production of type I IFN during herpes simplex virus (HSV) infect...

journal_title：Journal of virology

authors： Rasmussen SB,Sørensen LN,Malmgaard L,Ank N,Baines JD,Chen ZJ,Paludan SR

更新日期：2007-12-01 00:00:00

abstract：:Rift Valley fever virus (RVFV), a phlebovirus of the Bunyaviridae family, is an arthropod-borne virus which emerges periodically throughout Africa, emphasizing that it poses a major threat for animal and human populations. To assess the genetic variability of RVFV, several isolates from diverse localities of Africa we...

journal_title：Journal of virology

authors： Sall AA,Zanotto PM,Sene OK,Zeller HG,Digoutte JP,Thiongane Y,Bouloy M

更新日期：1999-10-01 00:00:00

abstract：:Respiratory syncytial virus (RSV) is a major cause of morbidity and mortality. Previous studies have suggested that T-cell responses may contribute to RSV immunopathology, which could be driven by dendritic cells (DCs). DCs are productively infected by RSV, and during RSV infections, there is an increase of DCs in the...

journal_title：Journal of virology

authors： Thornburg NJ,Shepherd B,Crowe JE Jr

更新日期：2010-12-01 00:00:00

abstract：:The influenza A virus nucleoprotein (NP) is a multifunctional polypeptide which plays a pivotal role in virus replication. To get information on the domains and specific residues involved in the different NP activities, we describe here the preparation and characterization of 20 influenza A virus mutant NPs. The mutat...

journal_title：Journal of virology

authors： Mena I,Jambrina E,Albo C,Perales B,Ortín J,Arrese M,Vallejo D,Portela A

更新日期：1999-02-01 00:00:00

abstract：:A rapid assay for retroviral reverse transcriptase activity released into the culture medium by infected cells was developed. With the assay, 4,000 clonally infected cell lines could be tested in a few hours. We have adapted the assay for use as a screen for the detection of spontaneous viral mutants. Mutants of Molon...

journal_title：Journal of virology

authors： Goff S,Traktman P,Baltimore D

更新日期：1981-04-01 00:00:00

abstract：:Although adeno-associated virus type 2 (AAV) has gained attention as a potentially useful vector for human gene therapy, the transduction efficiencies of AAV vectors vary greatly in different cells and tissues in vitro and in vivo. We have documented that a cellular tyrosine phosphoprotein, designated the single-stran...

journal_title：Journal of virology

authors： Qing K,Hansen J,Weigel-Kelley KA,Tan M,Zhou S,Srivastava A

更新日期：2001-10-01 00:00:00

abstract：:The complete nucleotide sequences of genomic RNA1 (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest identified positive-strand single-stranded RNA genome among plant vir...

journal_title：Journal of virology

authors： Kreuze JF,Savenkov EI,Valkonen JP

更新日期：2002-09-01 00:00:00