Replication-Competent NYVAC-KC Yields Improved Immunogenicity to HIV-1 Antigens in Rhesus Macaques Compared to Nonreplicating NYVAC.

Abstract:

:As part of the continuing effort to develop an effective HIV vaccine, we generated a poxviral vaccine vector (previously described) designed to improve on the results of the RV144 phase III clinical trial. The construct, NYVAC-KC, is a replication-competent, attenuated recombinant of the vaccinia virus strain NYVAC. NYVAC is a vector that has been used in many previous clinical studies but is replication deficient. Here, we report a side-by-side comparison of replication-restricted NYVAC and replication-competent NYVAC-KC in a nonhuman primate study, which utilized a prime-boost regimen similar to that of RV144. NYVAC-C and NYVAC-C-KC express the HIV-1 antigens gp140, and Gag/Gag-Pol-Nef-derived virus-like particles (VLPs) from clade C and were used as the prime, with recombinant virus plus envelope protein used as the boost. In nearly every T and B cell immune assay against HIV-1, including neutralization and antibody binding, NYVAC-C-KC induced a greater immune response than NYVAC-C, indicating that replication competence in a poxvirus may improve upon the modestly successful regimen used in the RV144 clinical trial.IMPORTANCE Though the RV144 phase III clinical trial showed promise that an effective vaccine against HIV-1 is possible, a successful vaccine will require improvement over the vaccine candidate (ALVAC) used in the RV144 study. With that goal in mind, we have tested in nonhuman primates an attenuated but replication-competent vector, NYVAC-KC, in direct comparison to its parental vector, NYVAC, which is replication restricted in human cells, similar to the ALVAC vector used in RV144. We have utilized a prime-boost regimen for administration of the vaccine candidate that is similar to the one used in the RV144 study. The results of this study indicate that a replication-competent poxvirus vector may improve upon the effectiveness of the RV144 clinical trial vaccine candidate.

journal_name

J Virol

journal_title

Journal of virology

authors

Kibler KV,Asbach B,Perdiguero B,García-Arriaza J,Yates NL,Parks R,Stanfield-Oakley S,Ferrari G,Montefiori DC,Tomaras GD,Roederer M,Foulds KE,Forthal DN,Seaman MS,Self S,Gottardo R,Phogat S,Tartaglia J,Barnett S,Cris

doi

10.1128/JVI.01513-18

subject

Has Abstract

pub_date

2019-01-17 00:00:00

issue

3

eissn

0022-538X

issn

1098-5514

pii

JVI.01513-18

journal_volume

93

pub_type

杂志文章
  • Overexpression of an alternatively spliced form of c-Myb results in increases in transactivation and transforms avian myelomonoblasts.

    abstract::An alternatively spliced form of c-myb exists that encodes an additional 120 amino acids in chicken and 121 amino acids in human and mouse. These amino acids are encoded by an additional exon, termed exon 9A. This exon is not present in v-myb, and proteins containing these amino acids have never been tested for oncoge...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.8.6813-6821.1998

    authors: Woo CH,Sopchak L,Lipsick JS

    更新日期:1998-08-01 00:00:00

  • Epitope mapping of monoclonal antibodies against human immunodeficiency virus type 1 structural proteins by using peptides.

    abstract::Murine monoclonal antibodies directed against the structural proteins p17 and p24 of human immunodeficiency virus type 1 were investigated in an epitope mapping system. Overlapping peptides consisting of 15 amino acids of the p17 and p24 protein, respectively, were used as competitors in an enzyme-linked immunosorbent...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.63.8.3525-3528.1989

    authors: Niedrig M,Hinkula J,Weigelt W,L'age-Stehr J,Pauli G,Rosen J,Wahren B

    更新日期:1989-08-01 00:00:00

  • Presentation of native epitopes in the V1/V2 and V3 regions of human immunodeficiency virus type 1 gp120 by fusion glycoproteins containing isolated gp120 domains.

    abstract::The immune response to viral glycoproteins is often directed against conformation- and/or glycosylation-dependent structures; synthetic peptides and bacterially expressed proteins are inadequate probes for the mapping of such epitopes. This report describes a retroviral vector system that presents such native epitopes...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.1.400-410.1994

    authors: Kayman SC,Wu Z,Revesz K,Chen H,Kopelman R,Pinter A

    更新日期:1994-01-01 00:00:00

  • Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro.

    abstract::RNA recombination occurs frequently during replication of tombusviruses and carmoviruses, which are related small plus-sense RNA viruses of plants. The most common recombinants generated by these viruses are either defective interfering (DI) RNAs or chimeric satellite RNAs, which are thought to be generated by templat...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.77.22.12033-12047.2003

    authors: Cheng CP,Nagy PD

    更新日期:2003-11-01 00:00:00

  • Molecular dissection of influenza virus nucleoprotein: deletion mapping of the RNA binding domain.

    abstract::Influenza virus nucleoprotein (NP) is associated with the genome RNA, forming ribonucleoprotein cores. To identify the amino acid sequence involved in RNA binding, we performed Northwestern blot analysis with a set of N- and C-terminal deletion mutants of NP produced in Escherichia coli. The RNA binding region has bee...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.12.8433-8436.1994

    authors: Kobayashi M,Toyoda T,Adyshev DM,Azuma Y,Ishihama A

    更新日期:1994-12-01 00:00:00

  • Role of herpes simplex virus 1 immediate early protein ICP22 in viral nuclear egress.

    abstract:UNLABELLED:In order to investigate the novel function(s) of the herpes simplex virus 1 (HSV-1) immediate early protein ICP22, we screened for ICP22-binding proteins in HSV-1-infected cells. Our results were as follows. (i) Tandem affinity purification of ICP22 from infected cells, coupled with mass spectrometry-based p...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01057-14

    authors: Maruzuru Y,Shindo K,Liu Z,Oyama M,Kozuka-Hata H,Arii J,Kato A,Kawaguchi Y

    更新日期:2014-07-01 00:00:00

  • Sequence analysis of the genome of the Neodiprion sertifer nucleopolyhedrovirus.

    abstract::The genome of the Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), which infects the European pine sawfly, N. sertifer (Hymenoptera: Diprionidae), was sequenced and analyzed. The genome was 86,462 bp in size. The C+G content of 34% was lower than that of the majority of baculoviruses. A total of 90 methionine-initi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.78.13.7036-7051.2004

    authors: Garcia-Maruniak A,Maruniak JE,Zanotto PM,Doumbouya AE,Liu JC,Merritt TM,Lanoie JS

    更新日期:2004-07-01 00:00:00

  • Requirements for kissing-loop-mediated dimerization of human immunodeficiency virus RNA.

    abstract::Sequences from the 5' end of type 1 human immunodeficiency virus RNA dimerize spontaneously in vitro in a reaction thought to mimic the initial step of genomic dimerization in vivo. Dimer initiation has been proposed to occur through a "kissing-loop" interaction involving a specific RNA stem-loop element designated SL...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.9.5902-5908.1996

    authors: Clever JL,Wong ML,Parslow TG

    更新日期:1996-09-01 00:00:00

  • Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein.

    abstract::Interactions between host factors and the viral replication complex play important roles in host adaptation and regulation of influenza virus replication. A cellular protein, nuclear factor 90 (NF90), was copurified with H5N1 viral nucleoprotein (NP) from human cells in which NP was transiently expressed and identifie...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00735-09

    authors: Wang P,Song W,Mok BW,Zhao P,Qin K,Lai A,Smith GJ,Zhang J,Lin T,Guan Y,Chen H

    更新日期:2009-08-01 00:00:00

  • Origin of the vaccinia virus hemagglutinin.

    abstract::The relationship between the vaccinia virus hemagglutinin and hemadsorption was examined. Hemagglutinin synthesis was temporally related to the appearance of the hemadsorption reaction. Only chicken erythrocytes, which reacted with hemagglutinin, hemadsorbed to infected cells, and both of these reactions were inhibite...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.9.2.290-296.1972

    authors: Blackman KE,Bubel HC

    更新日期:1972-02-01 00:00:00

  • Comparison of subacute sclerosing panencephalitis and measles viruses: an electron microscope study.

    abstract::The ultrastructure of CV-1 cells infected with subacute sclerosing panencephalitis (SSPE) viruses was compared with that of CV-1 cells infected with the wild or Edmonston strain of measles virus. Both SSPE viruses and the measles viruses produced two types of nucleocapsid structures: smooth filaments, 15 to 17 nm in d...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.7.1.176-187.1971

    authors: Oyanagi S,ter Meulen V,Katz M,Koprowski H

    更新日期:1971-01-01 00:00:00

  • Effects of nonleukemogenic and wild-type Moloney murine leukemia virus on lymphoid cells in vivo: identification of a preleukemic shift in thymocyte subpopulations.

    abstract::Infection of mice with Moloney murine leukemia virus (M-MuLV) as well as with a nonpathogenic variant, Mo+PyF101 M-MuLV, was studied. Mo+PyF101 M-MuLV differs from wild-type M-MuLV by the addition of enhancer sequences from polyomavirus in the long terminal repeat. Previous experiments indicated that Mo+PyF101 establi...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.60.2.423-430.1986

    authors: Davis BR,Chandy KG,Brightman BK,Gupta S,Fan H

    更新日期:1986-11-01 00:00:00

  • Herpesvirus saimiri episomal persistence is maintained via interaction between open reading frame 73 and the cellular chromosome-associated protein MeCP2.

    abstract::Herpesvirus saimiri (HVS) is the prototype gamma-2 herpesvirus, which naturally infects the squirrel monkey Saimiri sciureus, causing an asymptomatic but persistent infection. The latent phase of gamma-2 herpesviruses is characterized by their ability to persist in a dividing cell population while expressing a limited...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02171-06

    authors: Griffiths R,Whitehouse A

    更新日期:2007-04-01 00:00:00

  • Functional analysis of the core human immunodeficiency virus type 1 packaging signal in a permissive cell line.

    abstract::Packaging of type C retrovirus genomic RNAs into budding virions requires a highly specific interaction between the viral Gag precursor and unique cis-acting packaging signals on the full-length RNA genome, allowing the selection of this RNA species from among a pool of spliced viral RNAs and similar cellular RNAs. Th...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.72.7.5886-5896.1998

    authors: Harrison GP,Miele G,Hunter E,Lever AM

    更新日期:1998-07-01 00:00:00

  • Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple Ig heavy-chain lineages.

    abstract:UNLABELLED:An understanding of the antigen-specific B-cell response to the influenza virus hemagglutinin (HA) is critical to the development of universal influenza vaccines, but it has not been possible to examine these cells directly because HA binds to sialic acid (SA) on most cell types. Here, we use structure-based...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.03422-13

    authors: Whittle JR,Wheatley AK,Wu L,Lingwood D,Kanekiyo M,Ma SS,Narpala SR,Yassine HM,Frank GM,Yewdell JW,Ledgerwood JE,Wei CJ,McDermott AB,Graham BS,Koup RA,Nabel GJ

    更新日期:2014-04-01 00:00:00

  • The unique stacked rings in the nucleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found.

    abstract::One unique feature of the shrimp white spot syndrome virus (WSSV) genome is the presence of a giant open reading frame (ORF) of 18,234 nucleotides that encodes a long polypeptide of 6,077 amino acids with a hitherto unknown function. In the present study, by applying proteomic methodology to analyze the sodium dodecyl...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.1.140-149.2005

    authors: Leu JH,Tsai JM,Wang HC,Wang AH,Wang CH,Kou GH,Lo CF

    更新日期:2005-01-01 00:00:00

  • Convergent Co-option of the Retroviral gag Gene during the Early Evolution of Mammals.

    abstract::Endogenous retroviruses, records of past retroviral infections, are ubiquitous in vertebrate genomes. On occasion, vertebrate hosts have co-opted retroviral genes for their own biological functions. Here, we perform a phylogenomic survey of retroviral gag gene homologs within vertebrate genomes and identify two ancien...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00542-19

    authors: Wang J,Gong Z,Han GZ

    更新日期:2019-06-28 00:00:00

  • Pseudorabies virus triggers glycoprotein gE-mediated ERK1/2 activation and ERK1/2-dependent migratory behavior in T cells.

    abstract:UNLABELLED:The interaction between viruses and immune cells of the host may lead to modulation of intracellular signaling pathways and to subsequent changes in cellular behavior that are of benefit for either virus or host. ERK1/2 (extracellular signal regulated kinase 1/2) signaling represents one of the key cellular ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02549-14

    authors: Setas Pontes M,Devriendt B,Favoreel HW

    更新日期:2015-02-01 00:00:00

  • Serum- and calcium-induced differentiation of human keratinocytes is inhibited by the E6 oncoprotein of human papillomavirus type 16.

    abstract::Transfection of the E6 and E7 genes of the high-risk human papillomaviruses (HPVs) into primary genital keratinocytes generates colonies of proliferating cells which are resistant to calcium- and serum-induced terminal differentiation. To genetically map the HPV gene(s) responsible for this cellular phenotype, we clon...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.5.3269-3279.1996

    authors: Sherman L,Schlegel R

    更新日期:1996-05-01 00:00:00

  • Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components.

    abstract::The family of giant viruses is still expanding, and evidence of a translational machinery is emerging in the virosphere. The Klosneuvirinae group of giant viruses was first reconstructed from in silico studies, and then a unique member was isolated, Bodo saltans virus. Here we describe the isolation of a new member in...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01534-19

    authors: Bajrai LH,Mougari S,Andreani J,Baptiste E,Delerce J,Raoult D,Azhar EI,La Scola B,Levasseur A

    更新日期:2019-12-12 00:00:00

  • Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6.

    abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.13.6.1254-1262.1974

    authors: Van Etten JL,Vidaver AK,Koski RK,Burnett JP

    更新日期:1974-06-01 00:00:00

  • Herpes simplex virus type 1 oriL is not required for virus replication or for the establishment and reactivation of latent infection in mice.

    abstract::During the course of experiments designed to isolate deletion mutants of herpes simplex virus type 1 in the gene encoding the major DNA-binding protein, ICP8, a mutant, d61, that grew efficiently in ICP8-expressing Vero cells but not in normal Vero cells was isolated (P. K. Orberg and P. A. Schaffer, J. Virol. 61:1136...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.61.11.3528-3535.1987

    authors: Polvino-Bodnar M,Orberg PK,Schaffer PA

    更新日期:1987-11-01 00:00:00

  • Characterization of a herpes simplex virus type 2 75,000-molecular-weight glycoprotein antigenically related to herpes simplex virus type 1 glycoprotein C.

    abstract::Evidence is presented that the herpes simplex virus type 2 glycoprotein previously designated gF is antigenically related to herpes simplex virus type 1 gC (gC-1). An antiserum prepared against type 1 virion envelope proteins immunoprecipitated gF of type 2 (gF-2), and competition experiments revealed that the anti-gC...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.47.3.553-562.1983

    authors: Zezulak KM,Spear PG

    更新日期:1983-09-01 00:00:00

  • Influence of the rate of cell growth and cell density on interferon action in chick embryo cells.

    abstract::Chick embryo cells became more sensitive to the action of interferon the longer they remained in culture. This phenomenon was found even before confluency had been reached. The relative insensitivity of newly seeded cells was not due to a loss of receptors. Cells synthesizing deoxyribonucleic acid (DNA) at a high rate...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.4.1.7-11.1969

    authors: Rossman TG,Vilcek J

    更新日期:1969-07-01 00:00:00

  • Differential induction of antiviral effects against West Nile virus in primary mouse macrophages derived from flavivirus-susceptible and congenic resistant mice by alpha/beta interferon and poly(I-C).

    abstract::A cell model of primary macrophages isolated from the peritoneal cavity of flavivirus-susceptible and congenic resistant mice has been used to study the extent and kinetics of antiviral effects against West Nile virus upon priming with alpha/beta interferon (IFN-alpha/beta) or poly(I-C) (pIC). The pattern of flaviviru...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.3.1753-1764.2005

    authors: Pantelic L,Sivakumaran H,Urosevic N

    更新日期:2005-02-01 00:00:00

  • Respiratory syncytial virus (RSV) G glycoprotein is not necessary for vaccine-enhanced disease induced by immunization with formalin-inactivated RSV.

    abstract::Following respiratory syncytial virus (RSV) challenge, mice immunized with RSV G or with formalin-inactivated RSV (FI-RSV) exhibit severe disease associated with type 2 cytokine production and pulmonary eosinophilia. This has led to the proposal that the presence of RSV G is the factor in FI-RSV that induces disease-e...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.78.11.6024-6032.2004

    authors: Johnson TR,Teng MN,Collins PL,Graham BS

    更新日期:2004-06-01 00:00:00

  • Stable replication of papillomavirus genomes in Saccharomyces cerevisiae.

    abstract::Papillomaviruses normally replicate in stratified squamous epithelial tissues of their mammalian hosts, in which the viral genome is found as a nuclear plasmid. Two viral proteins, E1, a helicase, and E2, a transcriptional activator and plasmid maintenance factor, are known to contribute to the episomal replication of...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.7.3350-3358.2002

    authors: Angeletti PC,Kim K,Fernandes FJ,Lambert PF

    更新日期:2002-04-01 00:00:00

  • Herpes simplex virus immediate-early protein ICP22 is required for viral modification of host RNA polymerase II and establishment of the normal viral transcription program.

    abstract::Infection of cells with herpes simplex virus type 1 (HSV-1) results in a rapid alteration of phosphorylation on the large subunit of cellular RNA polymerase II (RNAP II), most likely on its C-terminal domain (S. A. Rice, M. C. Long, V. Lam, C. A. Spencer, J. Virol. 68:988-1001, 1994). This phosphorylation modification...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.9.5550-5559.1995

    authors: Rice SA,Long MC,Lam V,Schaffer PA,Spencer CA

    更新日期:1995-09-01 00:00:00

  • Analysis of protein levels of 24 cytokines in scrapie agent-infected brain and glial cell cultures from mice differing in prion protein expression levels.

    abstract::Activation of microglia and astroglia is seen in many neurodegenerative diseases including prion diseases. Activated glial cells produce cytokines as a protective response against certain pathogens and as part of the host inflammatory response to brain damage. In addition, cytokines might also exacerbate tissue damage...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01413-09

    authors: Tribouillard-Tanvier D,Striebel JF,Peterson KE,Chesebro B

    更新日期:2009-11-01 00:00:00

  • T-cell response to cottontail rabbit papillomavirus structural proteins in infected rabbits.

    abstract::Cottontail rabbit papillomavirus (CRPV)-induced papillomas progress at a high frequency to carcinomas and thus can serve as a model for high-cancer-risk human papillomavirus infection. Previously, we have shown that antibodies to nonstructural and structural proteins are detected in only a fraction of papilloma-bearin...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.68.6.4043-4048.1994

    authors: Selvakumar R,Borenstein LA,Lin YL,Ahmed R,Wettstein FO

    更新日期:1994-06-01 00:00:00