Abstract:
:Hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). In this report, we demonstrate that the cellular entry of HFRS-associated hantaviruses is facilitated by specific integrins expressed on platelets, endothelial cells, and macrophages. Infection of human umbilical vein endothelial cells and Vero E6 cells by the HFRS-causing hantaviruses Hantaan (HTN), Seoul (SEO), and Puumala (PUU) is inhibited by antibodies to alphavbeta3 integrins and by the integrin ligand vitronectin. The cellular entry of HTN, SEO, and PUU viruses, but not the nonpathogenic Prospect Hill (PH) hantavirus (i.e., a virus with no associated human disease), was also mediated by introducting recombinant alphaIIbbeta3 or alphavbeta3 integrins into beta3-integrin-deficient CHO cells. In addition, PH infectivity was not inhibited by alphavbeta3-specific sera or vitronectin but was blocked by alpha5beta1-specific sera and the integrin ligand fibronectin. RGD tripeptides, which are required for many integrin-ligand interactions, are absent from all hantavirus G1 and G2 surface glycoproteins, and GRGDSP peptides did not inhibit hantavirus infectivity. Further, a mouse-human hybrid beta3 integrin-specific Fab fragment, c7E3 (ReoPro), also inhibited the infectivity of HTN, SEO, and PUU as well as HPS-associated hantaviruses, Sin Nombre (SN) and New York-1 (NY-1). These findings indicate that pathogenic HPS- and HFRS-causing hantaviruses enter cells via beta3 integrins, which are present on the surfaces of platelets, endothelial cells, and macrophages. Since beta3 integrins regulate vascular permeability and platelet function, these findings also correlate beta3 integrin usage with common elements of hantavirus pathogenesis.
journal_name
J Viroljournal_title
Journal of virologyauthors
Gavrilovskaya IN,Brown EJ,Ginsberg MH,Mackow ERdoi
10.1128/JVI.73.5.3951-3959.1999subject
Has Abstractpub_date
1999-05-01 00:00:00pages
3951-9issue
5eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
杂志文章abstract::The product of the human cytomegalovirus (HCMV) UL71 gene is conserved throughout the herpesvirus family. During HCMV infection, protein pUL71 is required for efficient virion egress and is involved in the final steps of secondary envelopment leading to infectious viral particles. We found strong indications for oligo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06556-11
更新日期:2012-03-01 00:00:00
abstract::Several lines of evidence indicate that cutaneous human papillomavirus (HPV) types belonging to the beta genus of the HPV phylogenetic tree synergize with UV radiation in the development of skin cancer. Accordingly, the E6 and E7 oncoproteins from some beta HPV types are able to deregulate pathways related to immune r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01123-17
更新日期:2017-09-12 00:00:00
abstract::The development of cancer vaccines requires approaches to induce expansion and functional differentiation of tumor antigen-specific cytotoxic T lymphocyte (CTL) effectors which posses cytolytic capability and produce cytokines. Efficient induction of such cells is hindered by the poor immunogenicity of tumor antigens ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.13.7411-7424.2003
更新日期:2003-07-01 00:00:00
abstract::The 72-kDa immediate early 1 (IE1) protein encoded by human cytomegalovirus (hCMV) is a nuclearly localized promiscuous regulator of viral and cellular transcription. IE1 has long been known to associate with host mitotic chromatin, yet the mechanisms underlying this interaction have not been specified. In this study,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02606-13
更新日期:2014-01-01 00:00:00
abstract:UNLABELLED:Phosphoinositides and phosphoinositide binding proteins play a critical role in membrane and protein trafficking in eukaryotes. Their critical role in replication of cytoplasmic viruses has just begun to be understood. Poxviruses, a family of large cytoplasmic DNA viruses, rely on the intracellular membranes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03073-14
更新日期:2015-02-01 00:00:00
abstract::The small envelope protein of hepatitis B virus is the major component of the viral coat and is also secreted from cells as a 20-nm subviral particle, even in the absence of other viral proteins. Such empty envelope particles are composed of approximately 100 copies of this polypeptide and host-derived lipids and are ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4588-4597.1993
更新日期:1993-08-01 00:00:00
abstract::Herpes simplex virus type 1 glycoprotein H (HSV-1 gH) was synthesized in an inducible mammalian cell expression system, and its properties were examined. The gH coding sequence, together with the stable 5' untranslated leader sequence from xenopus beta-globin, was placed under control of the strong promoter from the h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4744-4755.1989
更新日期:1989-11-01 00:00:00
abstract:UNLABELLED:The potency and breadth of the recently isolated neutralizing human monoclonal antibodies to HIV-1 have stimulated interest in their use to prevent or to treat HIV-1 infection. Due to the antigenically diverse nature of the HIV-1 envelope (Env), no single antibody is highly active against all viral strains. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02097-15
更新日期:2015-12-01 00:00:00
abstract::Previous reports (H. Delius and J. B. Clements, J. Gen. Virol. 33:125-134, 1976; G. S. Hayward, R. J. Jacob, S. C. Wadsworth, and B. Roizman, Proc. Natl. Acad. Sci. U.S.A. 72:4243-4247, 1975; B. Roizman, G. S. Hayward, R. Jacob, S. W. Wadsworth, and R. W. Honess, Excerpta Med. Int. Congr. Ser. 2:188-198, 1974) have sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.2.448-457.1979
更新日期:1979-02-01 00:00:00
abstract::Human keratinocytes immortalized by full-length or early-region simian virus 40 (SV40) DNA grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the E6/E7 genes of human papillomaviruses. To determine the molecular basis for this biological difference in growth, we have used the in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10685-10691.2002
更新日期:2002-11-01 00:00:00
abstract::Mus dunni endogenous virus (MDEV) is activated from cells of the Asian wild mouse M. dunni (also known as Mus terricolor) in response to treatment with either 5-iodo-2'-deoxyuridine or hydrocortisone. MDEV represents a new murine retrovirus interference group and thus appears to use a different receptor for entry into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4663-4670.1997
更新日期:1997-06-01 00:00:00
abstract::The activation of the human polyomavirus BK causes polyomavirus-associated nephropathy in immunocompromised humans. Studies of the virus have been restricted since the virus DNA replication is species specific. Cell-based and cell-free DNA replication systems, including the BK virus (BKV) monopolymerase DNA replicatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00527-10
更新日期:2010-07-01 00:00:00
abstract::The mosquito-transmitted dengue virus (DENV) infects millions of people in tropical and subtropical regions. Maturation of DENV particles requires proper cleavage of the viral polyprotein, including processing of 8 of the 13 substrate cleavage sites by dengue virus NS2B/NS3 protease. With no available direct-acting an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00045-17
更新日期:2017-04-28 00:00:00
abstract::Proteins located in the tegument layer of herpesvirus particles play important roles in the replicative cycle at both early and late times after infection. As major constituents of the virion, they execute important functions in particular during formation of progeny virions. These functions have mostly been elucidate...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1332-1339.2006
更新日期:2006-02-01 00:00:00
abstract::Noroviruses account for 96% of viral gastroenteritis cases worldwide, with GII.4 strains responsible >80% of norovirus outbreaks. Histo-blood group antigens (HBGAs) are norovirus binding ligands, and antigenic and preferential HBGA binding profiles vary over time as new GII.4 strains emerge. The capsid P2 subdomain fa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06189-11
更新日期:2012-01-01 00:00:00
abstract::The characteristics of a rotaviruslike (SBR) virus isolated from striped bass (Morone saxatilis) were examined following purification of viruses from infected cell cultures. Virions had a double-layered capsid of icosahedral symmetry and a diameter of 75 nm. Purified viruses contained five polypeptides ranging in mole...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5235-5240.1990
更新日期:1990-11-01 00:00:00
abstract::A marked virus-inhibiting potency is obtained in the serum after intraperitoneal injection of polyacrylic acid (PAA) and polymethacrylic acid (PMAA) in mice. Much higher antiviral levels were reached than for other related polymers including dextran sulfate, heparin, polyvinyl sulfate, pyran copolymer, polystyrene sul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.2.9.886-893.1968
更新日期:1968-09-01 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) infected cell protein 0 (bICP0) stimulates productive infection, in part by activating viral gene expression. The C(3)HC(4) zinc RING finger of bICP0 is crucial for activating viral transcription and productive infection. In this study, we used a bacterial artificial chromosome containing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01348-08
更新日期:2008-12-01 00:00:00
abstract::The UL3 protein of equine herpesvirus 1 (EHV-1) KyA strain is a homolog of the ICP27 alpha regulatory protein of herpes simplex virus type 1 (HSV-1) and the ORF 4 protein of varicella-zoster virus. To characterize the regulatory function of the UL3 gene product, a UL3 gene expression vector (pSVUL3) and a vector expre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.5.2786-2793.1995
更新日期:1995-05-01 00:00:00
abstract::The immediate-early 1 and 2 (IE1 and IE2, respectively) proteins of human cytomegalovirus are known transcription factors, which regulate the expression of viral and cellular genes. Transcriptional activation by IE2 is dependent on the presence of a TATA motif in target promoters, and IE2 can interact directly with th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4028-4037.1996
更新日期:1996-06-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) infected cell protein 0 (ICP0) is a 110-kDa nuclear phosphoprotein that is required for both the efficient initiation of lytic infection and the reactivation of quiescent viral genomes from latency. The ability of ICP0 to act as a potent viral transactivator is mediated by its N-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01063-08
更新日期:2008-11-01 00:00:00
abstract::The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-05
更新日期:2006-07-01 00:00:00
abstract::A protocol was designed to measure the forward mutation rate over an entire gene replicated as part of a Moloney murine leukemia virus-based vector. For these studies, the herpes simplex virus thymidine kinase (tk) gene under the control of the spleen necrosis virus U3 promoter was used as target sequence since it all...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6357-6364.1993
更新日期:1993-11-01 00:00:00
abstract::This article has been retracted. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01824-07
更新日期:2008-01-23 00:00:00
abstract::Herpes simplex virus (HSV) glycoprotein B (gB) is an integral part of the multicomponent fusion system required for virus entry and cell-cell fusion. Here we investigated the mechanism of viral neutralization by the monoclonal antibody (MAb) 2c, which specifically recognizes the gB of HSV type 1 (HSV-1) and HSV-2. Bin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-10
更新日期:2011-02-01 00:00:00
abstract::The Arabidopsis flowering locus T (FT) gene encodes the mobile florigen essential for floral induction. While movement of the FT protein has been shown to occur within plants, systemic spread of FT mRNA remains to be unequivocally demonstrated. Utilizing novel RNA mobility assay vectors based on two distinct movement-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02346-08
更新日期:2009-04-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus that infects cells by fusing its lipid envelope with the target cell membrane. The fusion process requires the actions of viral glycoproteins gH, gL, and gB for entry into epithelial cells and additionally requires gp42 for entry into B cells. To further study the roles of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00572-06
更新日期:2006-10-01 00:00:00
abstract::The E7 oncoprotein of human papillomavirus 16 (HPV16) transforms basal and suprabasal cervical epithelial cells and is a tumor-specific antigen in cervical carcinoma, to which immunotherapeutic strategies aimed at cytotoxic T-lymphocyte (CTL) induction are currently directed. By quantifying major histocompatibility co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.5985-5997.2001
更新日期:2001-07-01 00:00:00
abstract::The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14446-14450.2005
更新日期:2005-11-01 00:00:00
abstract:UNLABELLED:Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying molecular mechanisms for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03070-13
更新日期:2014-03-01 00:00:00