Abstract:
:Herpes simplex virus type 1 (HSV-1) infected cell protein 0 (ICP0) is a 110-kDa nuclear phosphoprotein that is required for both the efficient initiation of lytic infection and the reactivation of quiescent viral genomes from latency. The ability of ICP0 to act as a potent viral transactivator is mediated by its N-terminal zinc-binding RING finger domain. This domain confers E3 ubiquitin ligase activity to ICP0 and is required for the proteasome-dependent degradation of a number of cellular proteins during infection, including the major nuclear domain 10 (ND10) constituent protein promyelocytic leukemia. In previous work we mapped three phosphorylation regions within ICP0, two of which directly affected its transactivation capabilities in transient transfection assays (Davido et al., J. Virol. 79:1232-1243, 2005). Because ICP0 is a phosphoprotein, we initially sought to test the hypothesis that phosphorylation regulates the E3 ubiquitin ligase activity of ICP0. Although none of the mutations affected ICP0 E3 ligase activity in vitro, transient transfection analysis indicated that mutations within one or more of the phosphorylated regions impaired the ability of ICP0 to form foci with colocalizing conjugated ubiquitin and to disrupt ND10. Mutations within one of the regions also affected ICP0 stability, and all of these phenomena occurred in a cell type-dependent manner. In the context of viral infection, only one ICP0 phosphorylation mutant (P1) showed a significant defect in viral replication and enhanced protein stability compared to all the other viruses tested. This study suggests that specific cellular environments and context of expression (transfection versus infection) differentially regulate several activities of ICP0 related to its E3 ubiquitin ligase activity via phosphorylation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Boutell C,Everett R,Hilliard J,Schaffer P,Orr A,Davido Ddoi
10.1128/JVI.01063-08subject
Has Abstractpub_date
2008-11-01 00:00:00pages
10647-56issue
21eissn
0022-538Xissn
1098-5514pii
JVI.01063-08journal_volume
82pub_type
杂志文章abstract::Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.299-305.1973
更新日期:1973-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.390-400.1981
更新日期:1981-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.7745-7753.1998
更新日期:1998-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01053-14
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abstract::Herpes simplex virus type 1 (HSV-1) B capsids are composed of seven proteins, designated VP5, VP19C, 21, 22a, VP23, VP24, and VP26 in order of decreasing molecular weight. Three proteins (21, 22a, and VP24) are encoded by a single open reading frame (ORF), UL26, and include a protease whose structure and function have...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5365-5374.1994
更新日期:1994-09-01 00:00:00
abstract::The human polyomavirus JC virus (JCV) is the causative agent of the fatal demyelinating disease progressive multifocal leukoencephalopathy (PML), which is commonly seen in AIDS patients. The bicistronic viral RNA, which is transcribed at the late phase of infection, is responsible for expressing the viral capsid prote...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02138-05
更新日期:2006-09-01 00:00:00
abstract::Hepatitis B virus (HBV) DNA clones were propagated from 57 carriers with antibody to hepatitis B e antigen (HBeAg) and sequenced within nucleotides (nt) 1685 to 1926 including the core promoter (nt 1742 to 1849) and the pre-C region (nt 1814 to 1900). Mutations in the core promoter or those in the pre-C region, or bot...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.68.12.8102-8110.1994
更新日期:1994-12-01 00:00:00
abstract::A tumorigenic line of mouse cells transformed by the nononcogenic murine K papovavirus was established and characterized. Tumor-bearing animals produced antibody specific for K virus-transformed cells; the K virus T antiserum did not react with polyomavirus-transformed cells. Immunoprecipitation of the T antigen in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.954-956.1984
更新日期:1984-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.12.9743-9752.1997
更新日期:1997-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.22.10683-10695.2001
更新日期:2001-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-07-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.2.290-296.1972
更新日期:1972-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4275-4283.1991
更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06059-11
更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jvi.74.6.2740-2751.2000
更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.70.7.4457-4465.1996
更新日期:1996-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1350-1356.1993
更新日期:1993-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.13.6242-6244.2001
更新日期:2001-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.299-307.1991
更新日期:1991-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6242-6251.1991
更新日期:1991-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.552-557.1988
更新日期:1988-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.405-410.1987
更新日期:1987-02-01 00:00:00