Abstract:
:In this study, we have extensively modified the Rb-binding domain of polyomavirus large T antigen. Mutant polyomavirus large T antigens were tested for their ability to bind pRb and p107 in vitro and assayed for their capacity to immortalize primary rat embryo fibroblasts in vivo. Polyomavirus large T antigen bound pRb and p107 through a common region located between amino acids 141 to 158, containing the consensus Rb-binding sequence D/N-L-X-C-X-E. Substitution of any amino acid within the core Rb-binding sequence abolished pRb and p107 binding in vitro and immortalization activity in vivo. Substitution of amino acids outside the core Rb-binding sequence reduced pRb and p107 binding in vitro and decreased or abolished immortalization of rat embryo fibroblasts in vivo. Although duplication of the Rb-binding domain within the polyomavirus large T antigen results in a molecule that can bind at least twice as much pRb and p107 in vitro, this mutant displayed an essentially wild-type level of immortalization activity. More importantly, we found that the addition of acidic residues within the casein kinase II consensus phosphorylation region flanking the Rb-binding domain, or the deletion of amino acids 256 to 272, increased the immortalizing activity of the mutant polyomavirus large T antigen. These two mutants displayed a greater than wild-type level of pRb binding in vitro, while in contrast, a decreased affinity for p107 binding in vitro was observed. Together, these results indicate that while pRb binding appears to be an essential event for immortalization, there is no tight correlation between the frequency of immortalization and the absolute level of pRb binding in vitro, indicating that other large T antigen functions are important for cellular immortalization.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pilon AA,Desjardins P,Hassell JA,Mes-Masson AMdoi
10.1128/JVI.70.7.4457-4465.1996subject
Has Abstractpub_date
1996-07-01 00:00:00pages
4457-65issue
7eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Infection with human T-cell leukemia virus type I (HTLV-I) is associated with adult T-cell lymphoma/leukemia. This disease occurs in only a small minority of people infected with HTLV-I and manifests itself many years after infection. Therefore, it appears that a fine balance exists between HTLV-I and the host T-cell ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5522-5528.1993
更新日期:1993-09-01 00:00:00
abstract::We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.680-683.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5225-5231.1991
更新日期:1991-10-01 00:00:00
abstract::The NS5A protein of hepatitis C virus (HCV) plays an important but undefined role in viral RNA replication. NS5A has been proposed to be a three-domain protein, and the crystal structure of the well-conserved amino-terminal domain I has been determined. The remaining two domains of NS5A, designated domains II and III,...
journal_title:Journal of virology
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doi:10.1128/JVI.00328-07
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abstract::The genome of the Neodiprion sertifer nucleopolyhedrovirus (NeseNPV), which infects the European pine sawfly, N. sertifer (Hymenoptera: Diprionidae), was sequenced and analyzed. The genome was 86,462 bp in size. The C+G content of 34% was lower than that of the majority of baculoviruses. A total of 90 methionine-initi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.13.7036-7051.2004
更新日期:2004-07-01 00:00:00
abstract::Although xenotropic murine leukemia viruses cannot productively infect cells of laboratory mice, cells from various wild-derived mice can support replication of these viruses. Although the virus-sensitive wild mice generally lack all or most of the xenotropic proviral genes characteristic of inbred strains, susceptibi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.690-695.1985
更新日期:1985-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.7056-7061.1996
更新日期:1996-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00524-17
更新日期:2017-08-24 00:00:00
abstract::The phenotypes of a series of mutant human immunodeficiency virus type 1 proviruses with linker insertion and deletion mutations within the gag coding region were characterized. These mutants, with mutations in the matrix, capsid, and p2 coding regions, produced replication-defective virion particles with defects in t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8645-8652.1996
更新日期:1996-12-01 00:00:00
abstract::Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-gamma) in the presence of anti-IFN-beta antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-gamma in FLC is in part mediated by...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4130-4136.1991
更新日期:1991-08-01 00:00:00
abstract::Early in infection by bacteriophage T4, before replication has commenced, one can detect the presence of newly synthesized DNA which cosediments with parental phage DNA on sucrose gradients. As shown earlier (R. E. Murray and C. K. Mathews, 1969), some of this represents covalent attachment of new material to parental...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.847-857.1974
更新日期:1974-04-01 00:00:00
abstract::While a diversity of immunogens that elicit qualitatively different cellular immune responses are being assessed in clinical human immunodeficiency virus vaccine trials, the consequences of those varied responses for viral control remain poorly understood. In the present study, we evaluated the induction of virus-spec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00204-08
更新日期:2008-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.374-381.1976
更新日期:1976-08-01 00:00:00
abstract::Infection of quiescent cells by human cytomegalovirus (HCMV) elicits severe cell cycle deregulation, resulting in a G(1)/S arrest, which can be partly attributed to the inactivation of the anaphase-promoting complex (APC). As we previously reported, the premature phosphorylation of its coactivator Cdh1 and/or the diss...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01260-10
更新日期:2010-10-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6) infects >90% of the population and establishes a latent infection with asymptomatic episodes of reactivation. However, HHV-6 reactivation is associated with morbidity and sometimes mortality in immunocompromised patients. To date, control of the virus in healthy virus carriers and the failu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02321-18
更新日期:2019-06-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00482-18
更新日期:2018-06-13 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10752-10765.2000
更新日期:2000-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5227-5235.1997
更新日期:1997-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01286-12
更新日期:2012-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.3.860-871.1980
更新日期:1980-12-01 00:00:00
abstract::Measles virus (MV) interacts with cellular receptors on the surface of peripheral blood lymphocytes (PBL) which mediate virus binding and uptake. Simultaneously, the direct contact of the viral glycoproteins with the cell surface induces a negative signal blocking progression to the S phase of the cell cycle, resultin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4499-4505.2001
更新日期:2001-05-01 00:00:00
abstract::The molecular mechanisms responsible for random integration and gene targeting by recombinant adeno-associated virus (AAV) vectors are largely unknown, and whether vectors derived from autonomous parvoviruses transduce cells by similar pathways has not been investigated. In this report, we constructed vectors based on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13136-13145.2003
更新日期:2003-12-01 00:00:00
abstract::Anelloviruses are a group of single-stranded circular DNA viruses infecting humans and other animal species. Animal models combined with reverse genetic systems of anellovirus have not been developed. We report here the construction and initial characterization of full-length DNA clones of a porcine anellovirus, torqu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00175-12
更新日期:2012-06-01 00:00:00
abstract::The structures of canine parvovirus (CPV) and feline parvovirus (FPV) complexed with antibody fragments from eight different neutralizing monoclonal antibodies were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 to 18 A. The crystal structure of one of the Fab molecules ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02532-08
更新日期:2009-06-01 00:00:00
abstract::The parechoviruses differ in many biological properties from other picornaviruses, and their replication strategy is largely unknown. In order to identify the viral RNA replication complex in human parechovirus type 1 (HPEV-1)-infected cells, we located viral protein and RNA in correlation to virus-induced membrane al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8512-8523.2003
更新日期:2003-08-01 00:00:00
abstract::Ebola virus (EBOV) infections result in aggressive hemorrhagic fever in humans, with fatality rates reaching 90% and with no licensed specific therapeutics to treat ill patients. Advances over the past 5 years have firmly established monoclonal antibody (MAb)-based products as the most promising therapeutics for treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01548-18
更新日期:2019-02-19 00:00:00
abstract::Alternative splicing of adeno-associated virus type 2 (AAV2) P19-generated pre-mRNAs generates the small Rep proteins Rep52 and Rep40, which differ in their carboxyl termini. Both proteins are required for optimal packaging of AAV2 genomes. AAV5 Rep-encoding P19-generated transcripts are primarily polyadenylated withi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01961-09
更新日期:2010-01-01 00:00:00
abstract::Rift Valley fever virus (RVFV), a phlebovirus of the family Bunyaviridae, is a major public health threat in Egypt and sub-Saharan Africa. The viral and host cellular factors that contribute to RVFV virulence and pathogenicity are still poorly understood. All pathogenic RVFV strains direct the synthesis of a nonstruct...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1371-1377.2001
更新日期:2001-02-01 00:00:00