Abstract:
:In this study, we examined the ability of the hepatitis B virus (HBV) precore, envelope, and X gene products to modulate HBV replication in the livers of transgenic mice that replicate the virus. Hepatic HBV replication was not affected by overexpression of the envelope or X gene products when these animals were crossed with transgenic mice that express the corresponding viral genes in the hepatocyte. Overexpression of the precore protein, however, eliminated nucleocapsid particles from the cytoplasm of the hepatocytes and abolished HBV replication without affecting the hepatic steady-state content of pregenomic HBV RNA. These observations suggest that the precore protein can exert a dominant negative effect on HBV replication, presumably at the level of nucleocapsid particle maturation or stability, suggesting an important role for this enigmatic viral protein in the HBV life cycle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Guidotti LG,Matzke B,Pasquinelli C,Shoenberger JM,Rogler CE,Chisari FVdoi
10.1128/JVI.70.10.7056-7061.1996subject
Has Abstractpub_date
1996-10-01 00:00:00pages
7056-61issue
10eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Purified retrovirus Gag proteins can assemble in vitro into virus-like particles (VLPs) in the presence of RNA. It was shown previously that a Rous sarcoma virus Gag protein missing only the protease domain forms spherical particles resembling immature virions lacking a membrane but that a similar protein missing the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.21.10260-10268.2000
更新日期:2000-11-01 00:00:00
abstract::The coronavirus, mouse hepatitis virus strain JHM, causes acute and chronic neurological diseases in rodents. Here we demonstrate that two closely related virus variants, both of which cause acute encephalitis in susceptible strains of mice, cause markedly different diseases if mice are protected with a suboptimal amo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10260-10269.2003
更新日期:2003-10-01 00:00:00
abstract::A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte gro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.5068-5074.1993
更新日期:1993-08-01 00:00:00
abstract::We have analyzed the effects of reducing conditions on the folding of the spike (S) protein and on the intracellular transport of the membrane (M) protein of the mouse hepatitis coronavirus. These proteins differ in their potential to form disulfide bonds in the lumen of the endoplasmic reticulum (ER). Intrachain disu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7394-7401.1993
更新日期:1993-12-01 00:00:00
abstract::V3-glycan-targeting broadly neutralizing antibodies (bNAbs) are a focus of HIV-1 vaccine development. Understanding the viral dynamics that stimulate the development of these antibodies can provide insights for immunogen design. We used a deep-sequencing approach, together with neutralization phenotyping, to investiga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00828-17
更新日期:2017-08-24 00:00:00
abstract::The baculovirus Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) initiates apoptosis in diverse insects through events triggered by virus DNA (vDNA) replication. To define the proapoptotic pathway and its role in antivirus defense, we investigated the link between the host's DNA damage response (DDR) a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02246-12
更新日期:2012-12-01 00:00:00
abstract::Cytotoxic-T-lymphocyte (CTL) escape mutations undermine the durability of effective human immunodeficiency virus type 1 (HIV-1)-specific CD8(+) T cell responses. The rate of CTL escape from a given response is largely governed by the net of all escape-associated viral fitness costs and benefits. The observation that C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03233-12
更新日期:2013-04-01 00:00:00
abstract::Herpes simplex virus establishes latent infections in the nuclei of sensory neurons. These infections are characterized by the abundant expression of a series of 5' coterminal transcripts termed the latency-associated transcripts (LATs). Available evidence indicates that LAT expression is specifically regulated in lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7424-7432.1996
更新日期:1996-11-01 00:00:00
abstract::Retroviral DNA integration requires the activity of at least one viral protein, the integrase (IN) protein. We cloned and expressed the integrase gene of feline immunodeficiency virus (FIV) in Escherichia coli as a fusion to the malE gene and purified the IN fusion protein by affinity chromatography. The protein is ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1468-1474.1994
更新日期:1994-03-01 00:00:00
abstract::The human foamy virus (HFV) contains within the U3 region of its long terminal repeat (LTR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.11.6353-6357.1991
更新日期:1991-11-01 00:00:00
abstract::Mechanisms by which the plus-sense RNA genomes of picornaviruses are replicated remain poorly defined, but existing models do not suggest a role for sequences encoding the capsid proteins. However, candidate RNA replicons (delta P1 beta gal and delta P1Luc), representing the sequence of human rhinovirus 14 virus (HRV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1941-1952.1996
更新日期:1996-03-01 00:00:00
abstract::With the rationale that the neuropathological similarities between scrapie and Alzheimer's disease reflect convergent pathological mechanisms involving altered gene expression, we set out to identify molecular events involved in both processes, using scrapie as a model to study the time course of these changes. We dif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4759-4768.1991
更新日期:1991-09-01 00:00:00
abstract::The level of RNase activity in human interferon preparations was examined. Although sequential purification of interferon resulted in nearly a 300-fold increase in specific activity, RNase-specific activity remained more or less constant. The implications of this finding for the analyses of the mode of action of inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.1247-1248.1977
更新日期:1977-03-01 00:00:00
abstract::The vaccinia virus F10 protein is one of two virally encoded protein kinases. A phenotypic analysis of infections involving a tetracycline-inducible recombinant (vDeltaiF10) indicated that F10 is involved in the early stages of virion morphogenesis, as previously reported for the mutants ts28 and ts15. The proteins en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2171-2190.2005
更新日期:2005-02-01 00:00:00
abstract::This paper describes Q beta noninfectious particles produced at 41 degrees C in a streptomycin-resistant Escherichia coli mutant which is temperature sensitive for suppression of a nonsense codon. The noninfectious particles resembled Q beta under the electron microscope and contained coat protein molecules in an amou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.1107-1117.1979
更新日期:1979-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) is a ubiquitous human pathogen associated with Burkitt's lymphoma and nasopharyngeal carcinoma. Although the EBV genome harbors more than a hundred genes, a full transcription map with EBV polyadenylation profiles remains unknown. To elucidate the 3' ends of all EBV transcripts genome-wide, we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01593-18
更新日期:2019-01-04 00:00:00
abstract::We have studied initiation and regulation of early transcription of simian virus (SV40) DNA in vitro by eucaryotic RNA polymerase II, using both a crude HeLa cell extract and a partially purified calf thymus polymerase supplemented with a HeLa cell S100 fraction. Analysis of initiation sites by primer-directed cDNA sy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.449-461.1982
更新日期:1982-02-01 00:00:00
abstract::The human papillomavirus (HPV) minor capsid protein L2 plays important roles in the generation of infectious viral particles and in the initial steps of infection. Here we show that HPV-16 L2 protein is sumoylated at lysine 35 and that sumoylation affects its stability. Interestingly, the sumoylated form of L2 cannot ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01269-10
更新日期:2010-11-01 00:00:00
abstract::Genetic and phylogenetic analyses suggest that the pandemic H1N1/2009 virus was derived from well-established swine influenza lineages; however, there is no convincing evidence that the pandemic virus was generated from a direct precursor in pigs. Furthermore, the evolutionary dynamics of influenza virus in pigs have ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01679-14
更新日期:2014-10-01 00:00:00
abstract::The 5'-untranslated regions (5' UTRs) of picornavirus genomes contain an internal ribosomal entry site (IRES) that promotes the end-independent initiation of translation. Picornavirus IRESs are classified into four structurally distinct groups, each with different initiation factor requirements. Here, we identify a fi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05862-11
更新日期:2012-02-01 00:00:00
abstract::Two human papillomavirus type 16 (HPV 16)-immortalized human keratinocyte cell lines (HPK) were shown to have retained the ability for differentiation after subcutaneous injection into nude mice. These properties were maintained even at late passage. HPK cells gave rise to transiently growing cysts which exhibited an ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.796-804.1991
更新日期:1991-02-01 00:00:00
abstract::Mice homozygous for mutant alleles at the gray tremor (gt) locus develop a marked non-intention tremor beginning at 8 days of age. Most homozygous mice die by 3 months. Homozygotes exhibit intense vacuolation of the central nervous system gray matter and vacuolation and hypomyelination of some white matter tracts. Bas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2342-2345.1997
更新日期:1997-03-01 00:00:00
abstract::To localize the epitopes recognized by monoclonal antibodies (MAbs) specific for the S1 subunit of the murine coronavirus JHMV spike protein, we have expressed S1 proteins with different deletions from the C terminus of S1. S1utt is composed of the entire 769-amino-acid (aa) S1 protein; S1NM, S1N, S1n(330), and S1n(22...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5403-5410.1994
更新日期:1994-09-01 00:00:00
abstract::Published work (D. J. McGeoch, Nucleic Acids Res. 18:4105-4110, 1990; J. E. McGeehan, N. W. Depledge, and D. J. McGeoch, Curr. Protein Peptide Sci. 2:325-333, 2001) has indicated that evolution of dUTPase in the class of herpesviruses that infect mammals and birds involved capture of a host gene followed by a duplicat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12880-12892.2005
更新日期:2005-10-01 00:00:00
abstract:UNLABELLED:The antiviral role of TRIM E3 ligases in vivo is not fully understood. To test the hypothesis that TRIM5α and TRIM22 have differential transcriptional regulation and distinct anti-HIV roles according to infection phase and compartment, we measured TRIM5α, TRIM22, and type I interferon (IFN-I)-inducible myxov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03603-13
更新日期:2014-04-01 00:00:00
abstract::We used a transfected L cell and a vaccinia vector carrying the herpes simplex virus type 1 (HSV-1) gene coding for glycoprotein D (gD) to characterize HSV-specific T-cell responses. Various studies with mice revealed that the vectors could stimulate some HSV-specific T-cell responses. Although the majority of the T c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.3.726-734.1987
更新日期:1987-03-01 00:00:00
abstract::The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.601-609.1977
更新日期:1977-02-01 00:00:00
abstract:UNLABELLED:Herpes simplex virus (HSV) replicates in the skin and mucous membranes, and initiates lytic or latent infections in sensory neurons. Assembly of progeny virions depends on the essential large tegument protein pUL36 of 3,164 amino acid residues that links the capsids to the tegument proteins pUL37 and VP16. O...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03167-15
更新日期:2016-05-12 00:00:00
abstract::A vaccine regimen that elicits broadly neutralizing antibodies (bNAbs) is a major goal in HIV-1 vaccine research. In this study, we assessed the immunogenicity of the CAP256 superinfecting viral envelope (CAP256 SU) protein delivered by modified vaccinia virus Ankara (MVA) and DNA vaccines in different prime-boost com...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02155-18
更新日期:2019-04-03 00:00:00
abstract::Two second-site mutations in Moloney murine leukemia virus envelope surface protein (SU) were previously shown to rescue infection of two different SU mutants, a fusion-defective point mutant and a fusion-defective modified SU that exhibits weak subunit association. We report here that they also rescue infection of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11881-11885.2001
更新日期:2001-12-01 00:00:00