Abstract:
:The immediate-early 1 and 2 (IE1 and IE2, respectively) proteins of human cytomegalovirus are known transcription factors, which regulate the expression of viral and cellular genes. Transcriptional activation by IE2 is dependent on the presence of a TATA motif in target promoters, and IE2 can interact directly with the TATA-binding protein (TBP) component of TFIID. TBP is known to be the target for transcriptional repression by the cellular Dr1 protein, and this factor has been shown to repress expression from the hsp70 promoter in vivo. Since this promoter is up-regulated by IE2, we asked whether the effects of Dr1 can be overcome by IE2. We report here that IE2 can overcome Dr1-mediated repression of the hsp70 promoter in vivo and that IE2 can interact with Dr1 in vivo and in vitro. We also demonstrate a previously unreported activity of Dr1, inhibition of DNA binding by TBP, and show that IE2 is able to overcome this inhibition in vitro, suggesting a mechanism for the TATA dependency of IE2-mediated trans activation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Caswell R,Bryant L,Sinclair Jdoi
10.1128/JVI.70.6.4028-4037.1996subject
Has Abstractpub_date
1996-06-01 00:00:00pages
4028-37issue
6eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5239-5246.1994
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pub_type: 杂志文章
doi:10.1128/JVI.23.3.751-767.1977
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.663-666.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01294-12
更新日期:2013-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.70.1.159-164.1996
更新日期:1996-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1727-1734.1991
更新日期:1991-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00723-08
更新日期:2008-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7369-7378.2004
更新日期:2004-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7440-7449.1998
更新日期:1998-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.1.49-57.1973
更新日期:1973-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.746-749.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03024-12
更新日期:2013-06-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.00895-12
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.3.598-607.1977
更新日期:1977-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.44.2.458-466.1982
更新日期:1982-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01573-15
更新日期:2015-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5850-5856.2005
更新日期:2005-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5883-5890.1990
更新日期:1990-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01177-06
更新日期:2006-11-01 00:00:00
abstract::Reverse transcription in avian sarcoma virus (ASV) initiates from the 3' end of a tRNA(Trp) primer, which anneals near the 5' end of the RNA genome. The region around the primer-binding site (PBS) forms an elaborate stem structure composed of the U5-inverted repeat (U5-IR) stem, the U5-leader stem, and the association...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7571-7577.2002
更新日期:2002-08-01 00:00:00
abstract::Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.13082-13093.2005
更新日期:2005-10-01 00:00:00