Abstract:
:We have analyzed the effects of reducing conditions on the folding of the spike (S) protein and on the intracellular transport of the membrane (M) protein of the mouse hepatitis coronavirus. These proteins differ in their potential to form disulfide bonds in the lumen of the endoplasmic reticulum (ER). Intrachain disulfide bonds are formed in the S protein but not in M, which was demonstrated in a pulse-chase experiment by analyzing the viral proteins under nonreducing conditions. To reduce disulfide bonds in vivo, we added dithiothreitol (DTT) to the culture medium of mouse hepatitis coronavirus-infected cells following a procedure recently described by Braakman et al. (I. Braakman, J. Helenius, and A. Helenius, EMBO J. 11:1717-1722, 1992). Short exposure to DTT resulted in the complete reduction of newly synthesized S protein and affected its conformation as judged by the change in mobility in nonreducing gels and by the loss of recognition by a conformation-specific monoclonal antibody. Using this antibody in an immunofluorescence assay, we monitored the reducing effect of DTT in situ. DTT was found to initially affect only the S protein present in the ER; also, after longer treatment, the remaining signal also gradually disappeared. In contrast, folding and transport of the M protein were not inhibited by DTT. Under reducing conditions, M was transported efficiently to the trans side of the Golgi complex, indicating that cellular processes such as ER-to-Golgi transport, O-glycosylation, and Golgi retention were unaffected. In the presence of DTT, the M protein even moved at an increased rate to the Golgi complex, which is probably because of its failure to interact with unfolded S protein. The effects of in vivo reduction were reversible. When DTT was removed from pulse-labeled cells, the S protein folded posttranslationally and aberrantly; during its oxidation, most of S now transiently aggregated into large disulfide-linked complexes from which subsequently folded S molecules dissociated.
journal_name
J Viroljournal_title
Journal of virologyauthors
Opstelten DJ,de Groote P,Horzinek MC,Vennema H,Rottier PJdoi
10.1128/JVI.67.12.7394-7401.1993subject
Has Abstractpub_date
1993-12-01 00:00:00pages
7394-401issue
12eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract::The ability of vaccinia virus to replicate in HeLa cells which had been previously infected with adenovirus type 2 (Ad2) was studied in order to gain insight into the mechanism by which adenovirus inhibits the expression of host cell functions. Vaccinia virus was employed in these studies because it replicates in the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.208-213.1971
更新日期:1971-02-01 00:00:00
abstract::Equine infectious anemia virus was found to be comprised of fourteen polypeptides of molecular weight ranging from 10,000 to 79,000. Eighty percent of the virion protein was accounted for by five polypeptides, including two non-glycosylated components (p29 and p13) comprising one-half of the virion protein and three g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.3.997-1001.1978
更新日期:1978-12-01 00:00:00
abstract::Bacteriophage phi105 is a temperate bacteriophage for Bacillus subtilis 168. Temperature-sensitive and plaque mutants of phi105 were isolated. The results of two- and three-factor crosses with these mutants suggest the vegetative map of phi105 to be circular. The location of prophage phi105 between bacterial markers p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.1.38-44.1969
更新日期:1969-01-01 00:00:00
abstract::Virus-like particles were purified from stools of patients in an epidemic of hepatitis A in Germany. When reference MS-1 chimpanzee pre-inoculation and convalescent sera were used, the close serological relationship of the purified particles to well-known isolates of hepatitis A could be established. On the other hand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.1.40-47.1978
更新日期:1978-04-01 00:00:00
abstract::The effect of 5-bromodeoxyuridine (BrdU) on vaccinia virus-induced polypeptide synthesis in BSC-1 cells has been investigated. Most virus-induced pre- and post-replicative polypeptides were synthesized in concentrations of 5-bromodeoxyuridine that inhibited virus growth. The synthesis of a few post-replicative polypep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1131-1133.1976
更新日期:1976-06-01 00:00:00
abstract::Macrophages perform a central role in the pathogenesis of human immunodeficiency virus type 1 (HIV-1) infection and have been implicated as the cell type most prominent in the development of central nervous system impairment. In this study, we evaluated the effect of interaction between macrophages and endothelial cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2133-2139.1995
更新日期:1995-04-01 00:00:00
abstract::Two hundred seventeen field isolates of cucumber mosaic cucumovirus (CMV), sampled from 11 natural populations, were typed by RNase protection assay (RPA) using probes from the genomic RNAs of strains in subgroup I and in subgroup II of CMV strains. Most (85%) of the analyzed isolates belonged to subgroup I. For these...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.2.934-940.1997
更新日期:1997-02-01 00:00:00
abstract::Feline herpesvirus produces characteristic morphological alterations in feline kidney cells. Nucleocapsid particles are formed in infected nuclei and are enveloped as they pass through the modified inner nuclear membrane. Aggregates of dense granular material and filamentous structures also regularly appear in infecte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.5.617-623.1970
更新日期:1970-05-01 00:00:00
abstract::Streptomyces coelicolor is a model system for the study of Streptomyces, a genus of bacteria responsible for the production of many clinically important antibiotics. Here we report the genome sequence of ΦCAM, a new S. coelicolor generalized transducing bacteriophage, isolated from a soil sample originating from Linco...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02681-12
更新日期:2012-12-01 00:00:00
abstract::Cell-free translation of separated Sendai virus mRNA species identified the message for polypeptide M and suggested the identity of the message for polypeptide NP. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.1.411-414.1978
更新日期:1978-10-01 00:00:00
abstract::Vesicular stomatitis virus has the potential for very rapid evolution in the laboratory, but like many other arboviruses, it evolves at a relatively slow rate in the natural environment. Previous work showed that alternating replication in different cell types does not promote stasis. In order to determine whether oth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12236-12242.2004
更新日期:2004-11-01 00:00:00
abstract::In the influenza H5N1 virus incident in Hong Kong in 1997, viruses that are closely related to H5N1 viruses initially isolated in a severe outbreak of avian influenza in chickens were isolated from humans, signaling the possibility of an incipient pandemic. However, it was not possible to prepare a vaccine against the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8303-8307.1999
更新日期:1999-10-01 00:00:00
abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract::The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhib...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00440-12
更新日期:2012-09-01 00:00:00
abstract::Rotavirus infection is one of the most common causes of diarrheal illness in humans. In neonatal mice, rhesus rotavirus (RRV) can induce biliary atresia (BA), a disease resulting in inflammatory obstruction of the extrahepatic biliary tract and intrahepatic bile ducts. We previously showed that the amino acid arginine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00510-17
更新日期:2017-07-12 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) entry inhibitor eCD4-Ig is a fusion of CD4-Ig and a coreceptor-mimetic peptide. eCD4-Ig is markedly more potent than CD4-Ig, with neutralization efficiencies approaching those of HIV-1 broadly neutralizing antibodies (bNAbs). However, unlike bNAbs, eCD4-Ig neutralized al...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02011-17
更新日期:2018-05-29 00:00:00
abstract::The UL11 tegument protein of herpes simplex virus plays a critical role in the secondary envelopment; however, the mechanistic details remain elusive. Here, we report a new function of UL11 in the budding process in which it directs efficient acquisition of glycoprotein E (gE) via a direct interaction. In vitro bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05207-11
更新日期:2011-09-01 00:00:00
abstract::The papillomavirus E2 gene product plays a pivotal role in viral replication. E2 has multiple functions, including (i) transcriptional activation and repression of viral promoters and (ii) the enhancement of viral DNA replication. It was previously reported that E2 suppressed the growth of papillomavirus-positive cerv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.8.3752-3760.2000
更新日期:2000-04-01 00:00:00
abstract::A total of 51 restriction sites were recognized within the BK virus genome by the combination of 10 different restriction endonucleases. These sites were mapped and oriented relative to one another as well as to the five fragments generated by the digestion of BK virus DNA with HindIII and EcoRI. The result was a comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.915-925.1979
更新日期:1979-03-01 00:00:00
abstract::Infection of normal rat fibroblasts (FR 3T3) with the early tsa mutant of polyoma virus may lead to either the A or the N phenotype, tsa-A transformants, originally derived by agar selection, are not temperature dependent for maintenance of the transformed phenotype, whereas tsa-N transormants revert at high temperatu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.2.421-426.1978
更新日期:1978-11-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) evokes a strong immune response, but the virus persists. Polymorphisms within known antigenic sites result in loss of immune recognition and can be positively selected. Amino acid variation outside known HLA class I restricted epitopes can also enable immune escape by interf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00968-06
更新日期:2007-02-01 00:00:00
abstract::BALB/c JLS V9 cells recently infected with Harvey sarcoma virus-murine leukemia virus (HSV-MuLV) complex contained unintegrated HSV linear DNA of 6.0-kilobase pair mass. The cells also contained two HSV closed circular DNA species along with MuLV-encoded linear and closed circular DNA species. HSV 6.0-kilobase pair li...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.1.30-39.1979
更新日期:1979-10-01 00:00:00
abstract::We performed live cell visualization assays to directly assess the interaction between competing adeno-associated virus (AAV) and herpes simplex virus type 1 (HSV-1) DNA replication. Our studies reveal the formation of separate AAV and HSV-1 replication compartments and the inhibition of HSV-1 replication compartment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02476-06
更新日期:2007-05-01 00:00:00
abstract::Foxp3(+) CD4(+) regulatory T cells (Tregs) represent a highly suppressive T cell subset with well-characterized immunosuppressive effects during immune homeostasis and chronic infections, although the role of these cells in acute viral infections is poorly understood. The present study sought to examine the induction ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05685-11
更新日期:2012-03-01 00:00:00
abstract::The type I interferon (IFN) response is part of the first-line defense against viral infection. To initiate replication, viruses have developed powerful evasion strategies to counteract host IFN responses. In the present study, we found that the Japanese encephalitis virus (JEV) NS5 protein could inhibit double-strand...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00039-17
更新日期:2017-03-29 00:00:00
abstract::Human cytomegalovirus (HCMV) protein pUL38 has been shown to prevent premature cell death by antagonizing cellular stress responses; however, the underlying mechanism remains unknown. In this study, we identified the host protein ubiquitin-specific protease 24 (USP24) as an interaction partner of pUL38. Mutagenesis an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00191-18
更新日期:2018-06-13 00:00:00
abstract::Removal of nucleoside triphosphates from extracts prepared from bacteriophage T7-infected Escherichia coli results in a stringent requirement for added ATP to form infective phage particles by in vitro packaging of bacteriophage T7 DNA. Optimal packaging efficiency was achieved at a concentration of about 1.25 mM. Oth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.1.365-367.1982
更新日期:1982-07-01 00:00:00
abstract::The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.6.922-924.1971
更新日期:1971-12-01 00:00:00
abstract::The key role of the mitochondria in the regulation of cellular Ca2+ led to a study of mitochondrial Ca2+ uptake during the infection of chicken embryo cells with Semliki Forest virus. Mitochondrial Ca2+ uptake was stimulated during the first 5 h of infection but declined later in infection. The early stimulation sugge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.143-152.1979
更新日期:1979-01-01 00:00:00
abstract::Helicobacter pylori causes peptic ulcers and gastric cancer, which lead to significantly higher morbidity in Japan than elsewhere in the world. As bacteriophage (phage) and host bacteria coevolve, the study of H. pylori phages is important to extend understanding of the evolution and pathogenesis of H. pylori. Here we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01767-12
更新日期:2012-10-01 00:00:00