Abstract:
:Avian reovirus, an important avian pathogen, expresses eight structural and four nonstructural proteins. The structural sigmaA protein is a major component of the inner capsid, clamping together lambdaA building blocks. sigmaA has also been implicated in the resistance of avian reovirus to the antiviral action of interferon by strongly binding double-stranded RNA in the host cell cytoplasm and thus inhibiting activation of the double-stranded RNA-dependent protein kinase. We have solved the structure of bacterially expressed sigmaA by molecular replacement and refined it using data to 2.3-A resolution. Twelve sigmaA molecules are present in the P1 unit cell, arranged as two short double helical hexamers. A positively charged patch is apparent on the surface of sigmaA on the inside of this helix and mutation of either of two key arginine residues (Arg155 and Arg273) within this patch abolishes double-stranded RNA binding. The structural data, together with gel shift assay, electron microscopy, and sedimentation velocity centrifugation results, provide evidence for cooperative binding of sigmaA to double-stranded RNA. The minimal length of double-stranded RNA required for sigmaA binding was observed to be 14 to 18 bp.
journal_name
J Viroljournal_title
Journal of virologyauthors
Guardado-Calvo P,Vazquez-Iglesias L,Martinez-Costas J,Llamas-Saiz AL,Schoehn G,Fox GC,Hermo-Parrado XL,Benavente J,van Raaij MJdoi
10.1128/JVI.00733-08subject
Has Abstractpub_date
2008-11-01 00:00:00pages
11208-16issue
22eissn
0022-538Xissn
1098-5514pii
JVI.00733-08journal_volume
82pub_type
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