Immunity in chimpanzees chronically infected with hepatitis C virus: role of minor quasispecies in reinfection.

abstract：:The fate of polyomavirus genomes in stable transformants was examined in experiments in which rat or hamster cells were infected with mixed viral populations containing either two distinguishable wild types or a wild type plus a transformation-deficient mutant. The results demonstrate that in 90% of the cases in eithe...

journal_title：Journal of virology

authors： Oh SY,Amalfitano A,Friderici K,Chen MC,Fluck MM

更新日期：1990-03-01 00:00:00

abstract：:A cDNA fragment coding for poliovirus capsid polypeptide VP1 was inserted into a simian virus 40 (SV40) genome in the place of the SV40 VP1 gene and fused in phase to the 3' end of the VP2-VP3 genes. Simian cells were infected with the resulting hybrid virus in the presence of an early SV40 mutant used as a helper. In...

journal_title：Journal of virology

authors： Wychowski C,Benichou D,Girard M

更新日期：1987-12-01 00:00:00

abstract：:The barley stripe mosaic virus (BSMV) beta(b) gene product is the major viral nonstructural protein synthesized during early stages of the infection cycle and is required for systemic movement of the virus. To examine the biochemical properties of beta(b), a histidine tag was engineered at the amino terminus and the p...

journal_title：Journal of virology

authors： Donald RG,Lawrence DM,Jackson AO

更新日期：1997-02-01 00:00:00

abstract：:Retrovirus infection is normally limited to cells within a specific host range which express a cognate receptor that is recognized by the product of the env gene. We describe retrovirus infection of cells outside of their normal host range when the infection is performed in the presence of a replication-defective aden...

journal_title：Journal of virology

authors： Adams RM,Wang M,Steffen D,Ledley FD

更新日期：1995-03-01 00:00:00

abstract：:A characterization of the antigenic determinants (epitopes) of the glycoprotein (G) of infectious hematopoietic necrosis virus was made by expressing different regions of the G gene in Escherichia coli. A cDNA copy of the G gene was divided into four fragments by TaqI digestion, and the fragments were subcloned into p...

journal_title：Journal of virology

authors： Xu L,Mourich DV,Engelking HM,Ristow S,Arnzen J,Leong JC

更新日期：1991-03-01 00:00:00

abstract：:Nef is a regulatory gene product of human immunodeficiency virus type 1 (HIV-1) and other primate lentiviruses which enhances virion infectivity by an unknown mechanism. We report here that Nef is detectable at moderate levels in preparations of HIV-1 virions which lack active viral protease (PR). Significantly smalle...

journal_title：Journal of virology

authors： Bukovsky AA,Dorfman T,Weimann A,Göttlinger HG

更新日期：1997-02-01 00:00:00

abstract：:Sindbis virus nonstructural polyproteins and their cleavage products are believed to be essential components of viral RNA replication and transcription complexes. Although numerous studies have investigated the effect of mutations in nsP1-, nsP2-, and nsP4-coding regions on Sindbis virus-specific RNA synthesis, relati...

journal_title：Journal of virology

authors： LaStarza MW,Lemm JA,Rice CM

更新日期：1994-09-01 00:00:00

abstract：:Recent environmental and metagenomic studies have considerably increased the repertoire of archaeal viruses and suggested that they play important roles in nutrient cycling in the biosphere. However, very little is known about how they regulate their life cycles and interact with their hosts. Here, we report that the ...

journal_title：Journal of virology

authors： Chen B,Chen Z,Wang Y,Gong H,Sima L,Wang J,Ouyang S,Gan W,Krupovic M,Chen X,Du S

更新日期：2020-07-30 00:00:00

abstract：UNLABELLED:Herpes simplex virus 1 (HSV-1) glycoprotein B (gB)-specific CD8(+) T cells protect mice from herpes infection and disease. However, whether and which HSV-1 gB-specific CD8(+) T cells play a key role in the "natural" protection seen in HSV-1-seropositive healthy asymptomatic (ASYMP) individuals (who have neve...

journal_title：Journal of virology

authors： Khan AA,Srivastava R,Spencer D,Garg S,Fremgen D,Vahed H,Lopes PP,Pham TT,Hewett C,Kuang J,Ong N,Huang L,Scarfone VM,Nesburn AB,Wechsler SL,BenMohamed L

更新日期：2015-04-01 00:00:00

abstract：:Hepatitis B virus particles contain three related viral envelope proteins, the small, middle, and large S (surface) proteins. All three proteins contain the small S amino acid sequence at their carboxyl terminus. It is not clear which of these S proteins functions as the viral attachment protein, binding to a target c...

journal_title：Journal of virology

authors： Pontisso P,Petit MA,Bankowski MJ,Peeples ME

更新日期：1989-05-01 00:00:00

abstract：:The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyac...

journal_title：Journal of virology

authors： Mirza MA,Weber J

更新日期：1979-05-01 00:00:00

abstract：:pp60v-src is a nonreceptor protein tyrosine kinase that can transform both chicken and rodent fibroblasts. The src homology 2 (SH2) domain of this protein serves a critical role in the regulation of protein tyrosine kinase activity. The host range proteins pp60v-src-L, which contains a deletion of a highly conserved r...

journal_title：Journal of virology

authors： Woods KM,Verderame MF

更新日期：1994-11-01 00:00:00

abstract：:ICP0 is a transcriptional activating protein required for the efficient replication and reactivation of latent herpes simplex virus 1 (HSV-1). Multiple regions of ICP0 contribute its activity, the most prominent of which appears to be the RING finger, which confers E3 ubiquitin ligase activity. A region in the C termi...

journal_title：Journal of virology

authors： Ferenczy MW,Ranayhossaini DJ,Deluca NA

更新日期：2011-05-01 00:00:00

abstract：:The RNAs of replication-defective murine and primate type C transforming viruses were analyzed for the presence of nucleotide sequences homologous to the genomes of their respective helper type C viruses by using DNAs complementary (cDNA) to either the 5'-terminal (cDNA5') or total (cDNAtotal) nucleotide sequences of ...

journal_title：Journal of virology

authors： Tronick SR,Cabradilla CD,Aaronson SA,Haseltine WA

更新日期：1978-06-01 00:00:00

abstract：:The Kaposi's sarcoma-associated herpesvirus (KSHV) open reading frame 57 (ORF57)-encoded protein (Mta) is a multifunctional regulator of viral gene expression. ORF57 is essential for viral replication, so elucidation of its molecular mechanisms is important for understanding KSHV infection. ORF57 has been implicated i...

journal_title：Journal of virology

authors： Hunter OV,Sei E,Richardson RB,Conrad NK

更新日期：2013-04-01 00:00:00

abstract：:HIV-1 infectivity is strongly restricted by TRIM5α from certain primate species but has been described as being only marginally susceptible to human TRIM5α. In this study, we evaluated the effects of the modulation of human TRIM5α activity (pretreatment of target cells with alpha interferon, expression of a pre-miRNA ...

journal_title：Journal of virology

authors： Battivelli E,Lecossier D,Matsuoka S,Migraine J,Clavel F,Hance AJ

更新日期：2010-11-01 00:00:00

abstract：:Infectious bursal disease virus (IBDV) is a nonenveloped virus with an icosahedral capsid composed of two proteins, VP2 and VP3, that derive from the processing of the polyprotein NH(2)-pVP2-VP4-VP3-COOH. The virion contains VP1, the viral polymerase, which is both free and covalently linked to the two double-stranded...

journal_title：Journal of virology

authors： Chevalier C,Lepault J,Da Costa B,Delmas B

更新日期：2004-04-01 00:00:00

abstract：:Accurate and sensitive quantitation of infectious human immunodeficiency virus (HIV) has been difficult to achieve. In this report, a quantitative focal immunoassay (FIA) for HIV was developed using human HeLa cells rendered susceptible to HIV infection by introduction of the CD4 gene via a retrovirus vector. Infected...

journal_title：Journal of virology

authors： Chesebro B,Wehrly K

更新日期：1988-10-01 00:00:00

abstract：:We analyzed capsomeres of human papillomavirus type 16 (HPV16) consisting of the L1 major structural protein for their ability to trigger a cytotoxic T-cell (CTL) response. To this end, we immunized C57BL/6 mice and used the L1(165-173) peptide for ex vivo restimulation of splenocytes prior to analysis ((51)Cr release...

journal_title：Journal of virology

authors： Ohlschläger P,Osen W,Dell K,Faath S,Garcea RL,Jochmus I,Müller M,Pawlita M,Schäfer K,Sehr P,Staib C,Sutter G,Gissmann L

更新日期：2003-04-01 00:00:00

abstract：:The biological activity encoded in the putative protease gene (pro) of human T-cell leukemia virus type I was investigated by using a vaccinia virus expression vector. The 53-kilodalton gag precursor polyprotein was processed into the mature p19, p24, and p15 gag proteins when the gag and protease-coding sequence was ...

journal_title：Journal of virology

authors： Nam SH,Kidokoro M,Shida H,Hatanaka M

更新日期：1988-10-01 00:00:00

abstract：:We have investigated the ability of human immunodeficiency virus type 1 (HIV-1) and HIV-2 isolates to infect and replicate in primary human macrophages. Monocytes from blood donors were allowed to differentiate into macrophages by culture in the presence of autologous lymphocytes and human serum for 5 days before infe...

journal_title：Journal of virology

authors： Valentin A,Albert J,Fenyö EM,Asjö B

更新日期：1994-10-01 00:00:00

abstract：:An in vitro method which led to the transformation of hamster embryo fibroblasts after exposure to herpes simplex virus type 2 (HSV-2) inactivated with ultraviolet irradiation is described. The transformed cells (333-8-9) produced tumors when inoculated into newborn Syrian hamsters but not when injected into weanling ...

journal_title：Journal of virology

authors： Duff R,Rapp F

更新日期：1971-10-01 00:00:00

abstract：:The polyomavirus proteins VP1, VP2, and VP3 move from their cytoplasmic site of synthesis into the nucleus, where virus assembly occurs. To identify cellular or viral components which might control this process, we determined the distribution of VP1, VP2, and VP3 in a soluble fraction, a cytoplasmic cytoskeleton fract...

journal_title：Journal of virology

authors： Stamatos NM,Chakrabarti S,Moss B,Hare JD

更新日期：1987-02-01 00:00:00

abstract：:Three defense functions of immunoglobulin A (IgA), immune exclusion, intracellular neutralization, and virus excretion, were assessed in a measles virus model using polarized epithelial cells expressing the polymeric immunoglobulin receptor and monoclonal antibodies against the viral H and F envelope proteins and the ...

journal_title：Journal of virology

authors： Yan H,Lamm ME,Björling E,Huang YT

更新日期：2002-11-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) protease and its substrate, the assembly protein ICP35, are involved in virion maturation. Both proteins are encoded by a single open reading frame but are translated independently from 3'-coterminal mRNAs of different sizes and are in frame. The herpesvirus shell assembles arou...

journal_title：Journal of virology

authors： Robertson BJ,McCann PJ 3rd,Matusick-Kumar L,Preston VG,Gao M

更新日期：1997-02-01 00:00:00

abstract：:Cytomegaloviruses (CMVs) are members of the Betaherpesvirinae subfamily of the Herpesviridae, and their properties of latency, large DNA size, gene redundancy, and ability to be cloned as bacterial artificial chromosomes (BACs) suggest their utility as vaccine vectors. While the K181 strain of murine CMV (MCMV) is wid...

journal_title：Journal of virology

authors： Redwood AJ,Messerle M,Harvey NL,Hardy CM,Koszinowski UH,Lawson MA,Shellam GR

更新日期：2005-03-01 00:00:00

abstract：:The foot-and-mouth disease virus (FMDV) capsid protein precursor, P1-2A, is cleaved by 3C(pro) to generate VP0, VP3, VP1, and the peptide 2A. The capsid proteins self-assemble into empty capsid particles or viruses which do not contain 2A. In a cell culture-adapted strain of FMDV (O1 Manisa [Lindholm]), three differen...

journal_title：Journal of virology

authors： Gullberg M,Polacek C,Bøtner A,Belsham GJ

更新日期：2013-11-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome contains three origins of DNA replication, one copy of oriL and two copies of oriS. Although oriL and oriS are structurally different, they have extensive nucleotide sequence similarity and can substitute for each other to initiate viral DNA replication. A fundamental que...

journal_title：Journal of virology

authors： Hardwicke MA,Schaffer PA

更新日期：1997-05-01 00:00:00

abstract：:Herpes simplex virus (HSV) codes for several envelope glycoproteins, including glycoprotein G-2 (gG-2) of HSV type 2 (HSV-2), which are dispensable for replication in cell culture. However, clinical isolates which are deficient in such proteins occur rarely. We describe here five clinical HSV-2 isolates which were fou...

journal_title：Journal of virology

authors： Liljeqvist JA,Svennerholm B,Bergström T

更新日期：1999-12-01 00:00:00

abstract：:Recombinant vaccinia viruses containing the 22-kilodalton protein (matrixlike or 22K protein) or phosphoprotein gene from respiratory syncytial virus were constructed. These recombinant viruses expressed proteins which were immunoprecipitated by appropriate respiratory syncytial virus antibodies and comigrated with au...

journal_title：Journal of virology

authors： Openshaw PJ,Anderson K,Wertz GW,Askonas BA

更新日期：1990-04-01 00:00:00