Abstract:
:The uncoating of adenovirus type 2 and a temperature-sensitive mutant, tsl, was studied. HEp-2 cells were infected with 32P- OR 125I-labeled purified virions for various lengths of time, and the nuclear and cytoplasmic fractions were analyzed by sucrose gradient velocity sedimentation and sodium dodecyl sulfate-polyacryl-amide gel electrophoresis. Within 1 h of infection, virions were converted into three subviral structures: (1) subviral structures in the cytoplasm with a density greater than virions but which qualitatively still contained all virus polypeptides; (ii) corelike structures associated with both the nuclear and cytoplasmic fractions and composed of viral DNA and polypeptides VIa2, V and PVII; and (iii) putative DNA-terminal protein complexes in the nuclei. The kinetic and compartmentalization studies suggested that the DNA-terminal protein complex is the end product of uncoating. The virions which were synthesized by tsl at the nonpermissive temperature and contained the precursor polypeptides PVI and PVII were found to be blocked in uncoating at the corelike stage. This block in uncoating provides the explanation for the lack of infectivity of these virions. A model for the uncoating of adenovirus is proposed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Mirza MA,Weber Jdoi
10.1128/JVI.30.2.462-471.1979subject
Has Abstractpub_date
1979-05-01 00:00:00pages
462-71issue
2eissn
0022-538Xissn
1098-5514journal_volume
30pub_type
杂志文章abstract::The localization of H-1 viral replicative-form double-stranded DNA and progeny single-stranded DNA replication in parasynchronously infected, simian virus 40-transformed newborn human kidney cells was studied with high-resolution electron microscope autoradiography (80-nm silver grains). We analyzed wild-type H-1 and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.349-360.1978
更新日期:1978-01-01 00:00:00
abstract::The 86-kDa IE2 protein (IE86) of human cytomegalovirus (HCMV) can act as both an activator and a repressor of gene expression. The mechanisms for both of these functions are not well defined. It has recently been demonstrated that this protein has sequence-specific DNA binding properties: it interacts directly with a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4117-4125.1994
更新日期:1994-07-01 00:00:00
abstract::The influenza A virus nucleoprotein (NP) is a single-stranded RNA-binding protein that encapsidates the virus genome and has essential functions in viral-RNA synthesis. Here, we report the characterization of a temperature-sensitive (ts) NP mutant (US3) originally generated in fowl plague virus (A/chicken/Rostock/34)....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01424-08
更新日期:2009-01-01 00:00:00
abstract::Among prion infections, two scenarios of prion spread are generally observed: (i) early lymphoid tissue replication or (ii) direct neuroinvasion without substantial antecedent lymphoid amplification. In nature, cervids are infected with chronic wasting disease (CWD) prions by oral and nasal mucosal exposure, and studi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00077-17
更新日期:2017-04-28 00:00:00
abstract::The N terminus of the capsid protein (CA) undergoes a considerable conformational change when the human immunodeficiency virus (HIV) protease cleaves it free from the Pr55(Gag) polyprotein. This rearrangement is thought to facilitate the establishment of specific CA-CA interactions that are required for the formation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.14.8009-8018.2003
更新日期:2003-07-01 00:00:00
abstract::NSP5 (NS26), the product of rotavirus gene 11, is a phosphoprotein whose role in the virus replication cycle is unknown. To gain further insight into its function, we obtained monoclonal antibodies against the baculovirus-expressed protein. By immunoprecipitation and immunoblotting experiments, we showed that (i) NSP5...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.34-41.1997
更新日期:1997-01-01 00:00:00
abstract::The replication of the single-stranded DNA (ssDNA) of parvovirus LuIII was studied in synchronized HeLa cells. After infection of the cells in early S phase, synthesis of a replicative form (RF) DNA became detectable as early as 9 h postinfection, i.e., after display of the cellular helper function(s) indispensable fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.841-853.1976
更新日期:1976-03-01 00:00:00
abstract::An R638A mutation of the polymerase acidic protein (PA) subunit of the RNA polymerase of influenza A/WSN/33 virus results in severe attenuation of viral growth in cell culture by promoting the synthesis of defective interfering RNAs. We propose that R638A is an "elongation" mutant that destabilizes PA-RNA template int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.8.5017-5020.2003
更新日期:2003-04-01 00:00:00
abstract::The Tf1 retrotransposon of Schizosaccharomyces pombe represents a group of eukaryotic long terminal repeat (LTR) retroelements that, based on their sequences, were predicted to use an RNA self-primer for initiating reverse transcription while synthesizing the negative-sense DNA strand. This feature is substantially di...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01370-08
更新日期:2008-11-01 00:00:00
abstract::The nodavirus flock house virus (FHV) has a bipartite, positive-sense, RNA genome that encodes the catalytic subunit of the RNA replicase and the viral capsid protein precursor on separate genomic segments (RNA1 and RNA2, respectively). RNA1 can replicate autonomously when transfected into permissive cells, allowing s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.7933-7942.1999
更新日期:1999-10-01 00:00:00
abstract::Infection and transformation of chondrocytes by Rous sarcoma viruses (RSVs) (Schmidt-Ruppin, Prague) stimulated the rate of cell growth. In contrast, several transformation-defective (td) mutants (tdPRA, tdNY105, tdNY106, tdNY107, and tdNY108) retaining various sizes of the src gene did not stimulate cell growth, indi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.2.531-541.1980
更新日期:1980-08-01 00:00:00
abstract::The region of the human cytomegalovirus (CMV) genome between the UL127 open reading frame and the major immediate-early (MIE) enhancer is referred to as the unique region. DNase I protection analysis with human cell nuclear extracts demonstrated multiple protein binding sites in this region of the viral genome (P. Gha...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9039-9052.1999
更新日期:1999-11-01 00:00:00
abstract::Mutagenesis of the transmembrane domain and cytoplasmic tail of human immunodeficiency virus type 1 envelope glycoprotein gp160 revealed that its intracellular transport and processing in transfected cell lines were modulated by a functional domain included in the carboxy-terminal sequence consisting of residues 751 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.3100-3103.1990
更新日期:1990-06-01 00:00:00
abstract::Multispecific CD8(+) T-cell responses are thought to be important for the control of acute hepatitis C virus (HCV) infection, but to date little information is actually available on the breadth of responses at early time points. Additionally, the influence of early therapy on these responses and their relationships to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.20.12979-12988.2005
更新日期:2005-10-01 00:00:00
abstract::The Epstein-Barr virus (EBV) nuclear antigen 3B (EBNA-3B) is considered nonessential for EBV-mediated B-cell growth transformation in vitro based on three virus isolates with EBNA-3B mutations. Two of these isolates could potentially express truncated EBNA-3B products, and, similarly, we now show that the third isolat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4506-4509.2005
更新日期:2005-04-01 00:00:00
abstract::Transfection has been found and characterized in pneumococcus. For replicating omega3 phage DNA extracted from infected cells, transfection was relatively efficient and rose linearly with DNA concentration and quadratically with time, according to T(T - 3.5) min(2). For mature DNA extracted from phage particles, trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.60-72.1978
更新日期:1978-01-01 00:00:00
abstract::The major internal structural protein of human T-cell lymphotropic virus type III (HTLV-III), a virus etiologically implicated in acquired immunodeficiency syndrome (AIDS), was purified to homogeneity. This 24,000-molecular-weight protein (p24) was shown to lack immunologic cross-reacting antigenic determinants shared...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.417-423.1985
更新日期:1985-08-01 00:00:00
abstract::The yeast two-hybrid system was used to catalog all detectable interactions among the P2 nonstructural cleavage products of poliovirus type 1 (Mahoney). Evidence has been obtained for specific associations among 2A(pro), 2BC, 2C, and 2B. Specifically, 2A(pro) can interact with itself and 2BC and its cleavage products ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1297-1307.1998
更新日期:1998-02-01 00:00:00
abstract::The p21 proteins of ras oncogenes are synthesized as precursors in the cytosol. After processing, which involves acylation, the products are associated with the plasma membrane in eucaryotic cells. The p21 overproduced in Escherichia coli, however, is not processed by acylation. A synthetic tetrapeptide of the p21 C t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.2.607-612.1985
更新日期:1985-11-01 00:00:00
abstract::Herpes simplex virus type 2 (HSV-2) glycoprotein B (gB-2) gene segments were expressed as recombinant proteins in Escherichia coli. gB-2 recombinant proteins were reacted with human serum immunoglobulin G (IgG) antibodies in Western immunoblot assays. Initially, samples were tested for the presence of HSV-1-specific a...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.70.5.2950-2956.1996
更新日期:1996-05-01 00:00:00
abstract::Interferons inhibit the replication of vesicular stomatitis virus (VSV), but not of encephalomyocarditis virus (EMCV), in mouse JLSV-11 cells. We report the isolation of clonal derivatives from this cell line in which the replication of both viruses is impaired by interferons. These clones were selected from the paren...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3175-3181.1988
更新日期:1988-09-01 00:00:00
abstract::Efforts to induce broadly reactive immunity against human immunodeficiency virus type 1 (HIV-1) have been impaired by the extent of sequence variation exhibited by this lentivirus. Cytotoxic T lymphocytes (CTL) specific for other viruses such as influenza virus have been shown to mediate immunity against divergent vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3145-3153.1994
更新日期:1994-05-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of PRRS, which has important impacts on the pig industry. PRRSV infection results in disruption of the swine leukocyte antigen class I (SLA-I) antigen presentation pathway. In this study, highly pathogenic PRRSV (HP-PRRSV) infection inh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01755-16
更新日期:2017-02-14 00:00:00
abstract::We developed a mouse model of Borna disease to facilitate immunopathogenesis research by adaptation of Borna disease virus to mice through serial passage in mouse brain tissue. Borna disease virus replication, antibody production, inflammation, and Borna disease expression in several different strains of mice were exa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.548-552.1993
更新日期:1993-01-01 00:00:00
abstract:UNLABELLED:The latent infection of Epstein-Barr virus (EBV) is associated with 1% of human cancer incidence. Poly(ADP-ribosyl)ation (PARylation) is a posttranslational modification catalyzed by poly(ADP-ribose) polymerases (PARPs) that mediate EBV replication during latency. In this study, we detail the mechanisms that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01180-16
更新日期:2016-09-12 00:00:00
abstract::Nidoviruses (arteriviruses, coronaviruses, and roniviruses) are a phylogenetically compact but diverse group of positive-strand RNA viruses that includes important human and animal pathogens. Nidovirus RNA synthesis is mediated by a cytoplasmic membrane-associated replication/transcription complex that includes up to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00261-09
更新日期:2009-06-01 00:00:00
abstract:UNLABELLED:The brown planthopper (BPH), Nilaparvata lugens (Hemiptera:Delphacidae), is one of the most destructive insect pests of rice crops in Asia. Nudivirus-like sequences were identified during the whole-genome sequencing of BPH. PCR examination showed that the virus sequences were present in all of the 22 BPH pop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03166-13
更新日期:2014-05-01 00:00:00
abstract::Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1 (HIV-1) primary viruses. Here, we show that virion capture assays, under conditions commonly reported in the literature, give ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.353-365.2003
更新日期:2003-01-01 00:00:00
abstract::Some retroviruses with an extended repeat (R) region encode the polyadenylation signal within the R region such that this signal is present at both the 5' and 3' ends of the viral transcript. This necessitates differential regulation to either repress recognition of the 5' polyadenylation signal or enhance usage of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.81-91.1999
更新日期:1999-01-01 00:00:00
abstract::The degree of genetic heterogeneity of DNA virus populations in nature and its consequences for disease control are virtually unknown. The parvovirus minute virus of mice (MVMi) was used here to investigate (i) the frequency of antibody-escape mutants in populations of a DNA virus and (ii) the ability of a DNA virus t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.4.2701-2708.2003
更新日期:2003-02-01 00:00:00