Abstract:
:Synthetic peptides containing portions of a highly conserved region of retroviral transmembrane proteins of human and animal retroviruses were tested for their ability to inhibit lymphoproliferation to determine the minimum amino acid sequence required. The previously reported immunosuppression mediated by the peptide CKS-17 was confirmed and further localized to a sequence of eight residues essentially identical to the sequence present in the transmembrane protein gp21 of human T-lymphotropic virus types I and II (HTLV-I and -II). To substantiate the physiological relevance of the inhibition of lymphoproliferation observed with the synthetic peptides and to relate this activity to the intact protein, we purified the Rauscher murine leukemia virus transmembrane protein p15E by immunoaffinity chromatography and report that this purified component presented in the form of protein micelles inhibited the interleukin-2-dependent proliferation of the murine T-cell line CTLL-2 in a dose-dependent manner, with a half-maximal inhibitory dose (ID50) of approximately 16 nM. In comparison, the ID50 concentration of a recombinant form of p15E required to inhibit lymphoproliferation was approximately 2.2 microM. The results reported here support the hypothesis that the transmembrane protein gp21 of HTLV-I and -II participates in the mechanism of immunosuppression previously reported for the transmembrane proteins of feline leukemia virus and other animal retroviruses. Thus, the transmembrane protein of HTLV-I, the etiological agent of adult T-cell leukemia-lymphoma, may be partially responsible for the immunocompromised clinical course of this disease that results in fatal opportunistic infections in a majority of cases.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ruegg CL,Monell CR,Strand Mdoi
10.1128/JVI.63.8.3250-3256.1989subject
Has Abstractpub_date
1989-08-01 00:00:00pages
3250-6issue
8eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
杂志文章abstract::Reassortant viruses which possessed the hemagglutinin and neuraminidase genes of wild-type human influenza A viruses and the remaining six RNA segments (internal genes) of the avian A/Pintail/Alberta/119/79 (H4N6) virus were previously found to be attenuated in humans. To study the genetic basis of this attenuation, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.9.2857-2863.1987
更新日期:1987-09-01 00:00:00
abstract::Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, WI61, and HAL1166), simian (rhesus rotavi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.41-57.2002
更新日期:2002-01-01 00:00:00
abstract::H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat to both animal and human health. Current influenza vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains of Newcastle disease vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01514-06
更新日期:2007-01-01 00:00:00
abstract::Poliovirus-specific RNA-dependent RNA polymerase (3Dpol) was purified to apparent homogeneity. A single polypeptide of an apparent molecular weight of 63,000 catalyzes the synthesis of dimeric and monomeric RNA products in response to the poliovirion RNA template. Analysis of purified 3Dpol by two-dimensional electrop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4563-4568.1989
更新日期:1989-11-01 00:00:00
abstract::Hepatitis C virus core protein forms the viral nucleocapsid and plays a critical role in the formation of infectious particles. In this study, we demonstrate that the highly conserved residue G33, located within domain 1 of the core protein, is important for the production of cell culture-infectious virus (HCVcc). Ala...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05452-11
更新日期:2012-01-01 00:00:00
abstract::A receptor on YAC-1 cells, a mouse T-lymphoma cell line, bound all six serotypes of the group B coxsackieviruses (CVB). In addition, the cells produced infectious virus. Each of the CVB competed for the same receptor on YAC-1 cells. CVB3 bound relatively slowly to YAC-1 cells (k = 4 x 10(-11) min-1 cell-1), and there ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3105-3108.1989
更新日期:1989-07-01 00:00:00
abstract::Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-06
更新日期:2007-01-01 00:00:00
abstract::Murine leukemia viruses isolated from radiation-induced BALB/c leukemias were characterized with respect to viral proteins and RNA. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the viral structural proteins revealed that for p12, p15, p30, and gp70, three of four electrophoretic variants of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.661-670.1980
更新日期:1980-02-01 00:00:00
abstract::We describe the use of herpesvirus promoters to regulate the expression of a Sindbis virus replicon (SINrep/LacZ). We isolated cell lines that contain the cDNA of SINrep/LacZ under the control of a promoter from a herpesvirus early gene which requires regulatory proteins encoded by immediate-early genes for expression...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.3.1998-2005.1999
更新日期:1999-03-01 00:00:00
abstract::Herpes simplex virus (HSV) glycoprotein B (gB) is an integral part of the multicomponent fusion system required for virus entry and cell-cell fusion. Here we investigated the mechanism of viral neutralization by the monoclonal antibody (MAb) 2c, which specifically recognizes the gB of HSV type 1 (HSV-1) and HSV-2. Bin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01924-10
更新日期:2011-02-01 00:00:00
abstract::Toroviruses (family Coronaviridae, order Nidovirales) are enveloped, positive-stranded RNA viruses that have been implicated in enteric disease in cattle and possibly in humans. Despite their potential veterinary and clinical relevance, little is known about torovirus epidemiology and molecular genetics. Here, we pres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9567-9577.2003
更新日期:2003-09-01 00:00:00
abstract::A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.951-957.1979
更新日期:1979-12-01 00:00:00
abstract::Adenovirus type 35 (Ad35) is a group B adenovirus that has been isolated primarily from patients with acquired immunodeficiency syndrome and other immunodeficiency disorders. We have studied the interaction of this unique adenovirus with the immune system by analyzing Ad35 early viral proteins in infected HeLa cells. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3665-3671.1987
更新日期:1987-12-01 00:00:00
abstract::Using gel shift assays, we found that the human immunodeficiency virus type 1 (HIV-1) Tat protein (Tat-1) bound both HIV-1 and HIV-2 TAR RNAs with similar high affinities. In contrast, the HIV-2 Tat protein (Tat-2) bound only TAR-2 RNA with high affinity. We conclude that the weak in vivo activity of Tat-2 on the HIV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.1110-1121.1993
更新日期:1993-02-01 00:00:00
abstract::Mouse mammary tumor virus (MMTV) infects a number of different cell types, including mammary gland and lymphoid cells, in vivo. To identify the cellular receptor for this virus, a mouse cDNA expression library was transfected into Cos-7 monkey kidney cells, and those transfected cells able to bind virus were selected ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3066-3071.1998
更新日期:1998-04-01 00:00:00
abstract::The integrase protein of human immunodeficiency virus type 1 removes two nucleotides from the 3' ends of reverse-transcribed human immunodeficiency virus type 1 DNA (3' processing) and covalently inserts the processed ends into a target DNA (DNA strand transfer). Mutant integrase proteins that lack the amino-and/or ca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5911-5917.1994
更新日期:1994-09-01 00:00:00
abstract::Blattella germanica densovirus (BgDNV) is an autonomous parvovirus that infects the German cockroach. BgDNV possesses three mRNAs for NS proteins, two of which are splice variants of the unspliced transcript. The unspliced variant encodes open reading frame 5 (ORF5) (NS3), while NSspl1 encodes ORF3 (NS1) and ORF4 (NS2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05523-11
更新日期:2011-11-01 00:00:00
abstract::Polypeptides synthesized in Newcastle disease virus (NDV)-infected CHO cells in the absence of glycosylation were characterized. Incorporation of either [3H]mannose of [3H]glucosamine into NDV polypeptides was inhibited to greater than 99% by the antibiotic tunicamycin. Under these conditions, infected cells synthesiz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.36.1.171-180.1980
更新日期:1980-10-01 00:00:00
abstract::The genome of the human immunodeficiency virus type 1 (HIV-1) is highly heterogeneous. Some of this genomic variability is reflected in the biologic and serologic differences observed among various strains of HIV-1. To map the viral determinants that correlate with pathogenicity of the virus, recombinant viruses were ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4390-4398.1990
更新日期:1990-09-01 00:00:00
abstract::Antiviral innate immune responses can be triggered by accumulation of intracellular nucleic acids resulting from virus infections. Double-stranded RNA (dsRNA) can be detected by the cytoplasmic RNA helicase proteins RIG-I and MDA5, two proteins that share sequence similarities within a caspase recruitment domain (CARD...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01325-06
更新日期:2006-12-01 00:00:00
abstract::We previously reported that exosomal transfer of hepatitis C virus (HCV) positive-strand RNA from human Huh-7 hepatoma cells to human plasmacytoid dendritic cells (pDCs) triggers pDC alpha/beta interferon (IFN-α/β) production in a Toll-like receptor 7 (TLR7)-dependent, virus-independent manner. Here we show that human...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01714-13
更新日期:2014-01-01 00:00:00
abstract::Adenovirus class B (Ad3 and Ad7) and class C (Ad1, Ad5, and Ad6) late r-strand mRNA's were found to have segmented 5' leaders. These leaders were very similar among serotype within a class but differed in sequence from the leaders on late mRNA's of a different class. However, the leader components of class B viruses m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.30.3.899-912.1979
更新日期:1979-06-01 00:00:00
abstract::Circulating interferon production, induced by Newcastle disease virus, is about seven times higher in C(57) Black mice than i Balb/c/Gif mice. A Mendelian analysis was carried out and circulating interferon production was measured in reciprocal F(1) hybrids, in the F(2) generation, in progeny of backcrosses of F(1) hy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.5.506-512.1969
更新日期:1969-05-01 00:00:00
abstract:UNLABELLED:Recent experiments suggest that some glycoprotein (GP)-specific monoclonal antibodies (MAbs) can protect experimental animals against the filovirus Ebola virus (EBOV). There is a need for isolation of MAbs capable of neutralizing multiple filoviruses. Antibody neutralization assays for filoviruses frequently...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00101-16
更新日期:2016-03-28 00:00:00
abstract::We constructed vaccine vectors based on live recombinant vesicular stomatitis virus (VSV) and a Semliki Forest virus (SFV) replicon (SFVG) that propagates through expression of the VSV glycoprotein (G). These vectors expressing simian immunodeficiency virus (SIV) Gag and Env proteins were used to vaccinate rhesus maca...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00342-11
更新日期:2011-06-01 00:00:00
abstract:UNLABELLED:Human noroviruses (NoVs) cause acute epidemic gastroenteritis. Susceptibility to the majority of NoV infections is determined by genetically controlled secretor-dependent expression of histo-blood group antigens (HBGAs), which are also critical for NoV attachment to host cells. Human NoVs are classified into...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00201-14
更新日期:2014-06-01 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne alphavirus responsible for chikungunya fever. Nonstructural protein 2 (nsP2), a multifunctional protein essential for viral replication, has an N-terminal helicase region (nsP2h), which as both nucleotide triphosphatase and RNA triphosphatase activities, as well as a C-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01470-20
更新日期:2020-12-16 00:00:00
abstract::Replication of poliovirus (PV) is restricted to a few sites, including the brain and spinal cord. However, this neurotropism is not conserved in cultured cells. Monkey kidney cells become susceptible to PV infection after cultivation in vitro, and cell lines of monolayer cultures from almost any tissue of primates are...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.9.4313-4325.2006
更新日期:2006-05-01 00:00:00
abstract::The swine-specific coronavirus transmissible gastroenteritis virus (TGEV) uses pig aminopeptidase-N (pAPN) as a cellular receptor. We showed that the human aminopeptidase-N (hAPN) cannot substitute for pAPN in this respect, although the two enzymes have 80% amino acid sequence identity. In order to map the TGEV bindin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5216-5224.1994
更新日期:1994-08-01 00:00:00
abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) expresses IE86, IE72, IE55, and IE18 mRNA by differential splicing. Reverse transcription-PCR with IE72-specific primers generated an 0.65-kb cDNA from HCMV-infected fibroblast RNA, which does not correspond to any known MIE cDNA. Nucleotide sequenci...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.7.3158-3167.2002
更新日期:2002-04-01 00:00:00